Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica

   

Identification and comprehensive evaluation of salt-alkali tolerance throughout the growth period of 155 faba bean germplasms 

FAN Hui-Ling1,2,BAI Sheng-Wen2,LU Yan2,PENG Xiao-Xing1,ZHOU Xian-Li1,ZHANG Hong-Yan1,TENG Chang-Cai1,WU Xue-Xia1,*,LIU Yu-Jiao1,*   

  1. 1 Qinghai University, Xining 810016, Qinghai, China; 2 Hexi University, Zhangye 734000, Gansu, China
  • Received:2024-04-20 Revised:2024-08-15 Accepted:2024-08-15 Published:2024-08-29
  • Supported by:
    This study was supported by the China Agriculture Research System of MOF and MARA (Edible bean, CARS-08) and the National Natural Science Foundation of China (42267008).

PDF

9

Abstract:

Soil salinization has become one of the critical abiotic stresses affecting the growth of faba bean. Identifying salt-alkali tolerant germplasm in faba bean lays the foundation for exploring salt-alkali tolerant genes and selecting and breeding salt-alkali tolerant varieties, which is of great significance for the utilization of salinized land. In this study, 155 faba bean germplasm resources from domestic and international collections were subjected to stress treatment with a matrix + mixed salt-alkali (9 g L-1, NaCl+Na2CO3+Na2SO4, pH 10.5) throughout their entire growth period. Seven indicators were measured, including the seedling rate, salt damage index (SDI), plant height, fresh weight, dry weight, chlorophyll content, and nitrogen content. Correlation analysis, principal component analysis, membership function analysis, and systematic cluster analysis were used to comprehensively evaluate and classify the salt-alkali tolerance of various germplasms. A predictive regression equation for salt-alkali tolerance was established using stepwise regression analysis. The results showed that: (1) Three faba bean germplasms with salt-alkali tolerance (20 ≤ salt damage index < 40) were identified, constituting 1.94% of the total, while eight germplasms displayed moderate salt-alkali tolerance (40 ≤ salt damage index < 60), comprising 5.16% of the tested germplasms. No highly salt-alkali tolerant (salt damage index < 20) faba bean germplasms were detected. (2) The salt damage index was negatively correlated with plant height, fresh weight, dry weight, chlorophyll content, and nitrogen content (P < 0.01). (3) Five indicators—fresh weight, plant height, salt damage index, chlorophyll content, and seedling rate—can be used as evaluation criteria for assessing salt-alkali tolerance throughout the entire growth period of faba beans. (4) The 155 faba bean germplasms were divided into two major groups, with the salt-alkali tolerant germplasm group exhibiting higher seedling rates, biomass, nitrogen content, and chlorophyll content. These research findings provide reliable materials for studying the mechanisms of salt-alkali tolerance in faba beans, exploring salt-alkali tolerant genes, and selecting and breeding salt-alkali tolerant varieties.

Key words: faba bean (Vicia faba L.), whole growth period, salt-alkali stress, salt-alkali tolerant germplasm identification, comprehensive evaluation

[1] 孟繁昊, 王聪, 徐寿军. 盐胁迫对植物的影响及植物耐盐机理研究进展. 内蒙古民族大学学报(自然科学版), 2014, 29(3): 315–318.

Meng F H, Wang C, Xu S J. Advances in research on effects of salt stress on plant and the mechanism of plant salt tolerance. J Inn Mong Minzu Univ Nat Sci, 2014, 29(3): 315–318 (in Chinese with English abstract).

[2] 赵娜, 缪亚梅, 陈满峰, 王学军. 蚕豆耐盐性的研究进展. 安徽农业科学, 2015, 43(18): 20–21.

Zhao N, Miao Y M, Chen M F, Wang X J. Research progress on salt tolerance of faba bean. J Anhui Agric Sci, 2015, 43(18): 20–21 (in Chinese with English abstract).

[3] 乔枫, 罗桂花, 耿贵工. 蚕豆幼苗对NaCl和NaHCO3胁迫的生理响应. 安徽农业大学学报, 2011, 38: 783–787.

Qiao F, Luo G H, Geng G G. Physiological responses of Faba bean L. seedlings under NaCl and NaHCO3 stress. J Anhui Agric Univ, 2011, 38: 783–787 (in Chinese with English abstract). 

[4] 刘霞. Na2CO3胁迫对蚕豆幼苗生长及叶绿素含量的影响. 长江蔬菜, 2010, (12): 30–32.

Liu X. Effects of Na2CO3 stress on growth and chlorophyll content of Vicia faba L. J Changjiang Veg, 2010, (12): 30–32(in Chinese with English abstract).

[5] 杨访问. 盐胁迫下蚕豆萌发期转录组及LEA基因家族分析. 长江大学硕士学位论文, 湖北荆州, 2020.

Yang F W. Transcriptome Profile of Faba Bean during Seed Germination under Salt Stress and Analysis of LEA Gene Family. MS Thesis of Yangtze University, Jingzhou, Hubei, China, 2020 (in Chinese with English abstract).

[6] 朱志华, 王明珍, 宋景芝, 张晓芳. 蚕豆和豇豆品种耐盐性鉴定初报. 作物品种资源, 1990, (4): 29–30.

Zhu Z H, Wang M Z, Song J Z, Zhang X F. Preliminary report on salt tolerance identification of faba bean and cowpea varieties. Crop Var Resour, 1990, (4): 29–30 (in Chinese with English abstract).

[7] 郝振萍, 金潇潇, 王长义, 王倩, 赵辉. 鲜食蚕豆苗期耐盐品种的筛选. 江苏农业科学, 2016, 44(12): 198–200.

Hao Z P, Jin X X, Wang C Y, Wang Q, Zhao H. Screening of salt-tolerant varieties of fresh faba bean at seedling stage. Jiangsu Agric Sci, 2016, 44(12): 198–200 (in Chinese with English abstract).

[8] 樊有存. 蚕豆耐盐种质资源筛选与抗盐基因的克隆及表达分析. 青海大学硕士学位论文, 青海西宁, 2021.

Fan Y C. Screening of Salt-tolerant Germplasm Resources and Cloning and Expression Analysis of Salt-tolerant Genes in Faba bean L. MS Thesis of Qinghai University, Xining, Qinghai, China, 2021 (in Chinese with English abstract).

[9] Bimurzayev N, Sari H, Kurunc A, Doganay K H, Asmamaw M. Effects of different salt sources and salinity levels on emergence and seedling growth of faba bean genotypes. Sci Rep, 2021,11: 18198.

[10] 宗绪晓, 包世英, 关建平. 蚕豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006.

Zong X X, Bao S Y, Guan J P. Descriptors and Data Standard for Faba Bean. Beijing: China Agriculture Press, 2006 (in Chinese).

[11] 阎旭东. 植物耐盐性鉴定及评价技术规程. 北京: 中国农业科学技术出版社, 2012.

Yan X D. Technical Specifications for the Identification and Evaluation of Plant Salt Tolerance. Beijing: China Agricultural Science and Technology Press, 2012 (in Chinese).

[12] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选. 作物学报, 2022, 48: 367–379.

Hu L L, Wang S H, Wang L X, Cheng X Z, Chen H L. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage. Acta Agron Sin, 2022, 48: 367–379 (in Chinese with English abstract).

[13] 薛天源, 鲁金春子, 何思晓, 余忆, 陈敬东, 文静, 沈金雄, 傅廷栋, 曾长立, 万何平. 286份甘蓝型油菜种质苗期耐盐碱性综合评价. 植物遗传资源学报, 2024, 25: 356–372.

Xue T Y, Lu J C Z Z, He S X, Yu Y, Chen J D, Wen J, Shen J X, Fu T D, Zeng C L, Wan H P. Comprehensive evaluation on saline-alkali tolerance of 286 Brassica napus germplasm at seedling stage. J Plant Genet Resour, 2024, 25: 356–372 (in Chinese with English abstract).

[14] 陈二影, 王润丰, 秦岭, 杨延兵, 黎飞飞, 张华文, 王海莲, 刘宾, 孔清华, 管延安. 谷子芽期耐盐碱综合鉴定及评价. 作物学报, 2020, 46: 1591–1604.

Chen E Y, Wang R F, Qin L, Yang Y B, Li F F, Zhang H W, Wang H L, Liu B, Kong Q H, Guan Y A. Comprehensive identification and evaluation of foxtail millet for saline-alkaline tolerance during germination. Acta Agron Sin, 2020, 46: 1591–1604 (in Chinese with English abstract).

[15] 张得芳, 樊光辉, 马玉林. 柴达木盆地盐碱土壤类型及其盐离子相关性研究. 青海农林科技, 2016, (3): 1–6.

Zhang D F, Fan G H, Ma Y L. Study on the type of saline-alkaline land and saltion correlation of Qaidam Basin. Sci Technol Qinghai Agric For, 2016, (3): 1–6 (in Chinese with English abstract).

[16] 邵桂花. 大豆种质资源耐盐性田间鉴定方法. 作物杂志, 1986, (3): 36–37.

Shao G H. Method for field identification of salt tolerance of soybean germplasm resources. Crops, 1986, (3): 36–37 (in Chinese).

[17] 姜奇彦, 胡正, 张辉, 王萌萌, 唐俊源, 倪志勇, 姜锋. 大豆种质资源耐盐性鉴定与研究. 植物遗传资源学报, 2012, 13: 726–732.

Jiang Q Y, Hu Z, Zhang H, Wang M M, Tang J Y, Ni Z Y, Jiang F. Evaluation for salt tolerance in soybean cultivars(Glycine max L.Merrill). J Plant Genet Resour, 2012, 13: 726–732 (in Chinese with English abstract).

[18] 邵桂花, 宋景芝, 刘惠令. 大豆种质资源耐盐性鉴定初报. 中国农业科学, 1986, 19(6): 30–35.

Shao G H, Song J Z, Liu H L. Preliminary studies on the evalution of salt tolerance in soybean varieties. Sci Agric Sin, 1986, 19(6): 30–35 (in Chinese with English abstract).

[19] 时丽冉. 盐胁迫下蚕豆幼苗抗氧化酶动态变化研究. 农业科技通讯, 2009, (3): 35–37.

Shi L R. The research of antioxidant enzymes activity dynamic change of Vicia faba L. seedling under salt-stress. Bull Agric Sci Technol, 2009, (3): 35–37 (in Chinese with English abstract).

[20] 时丽冉, 曹永胜, 郭盼. 盐胁迫对蚕豆植株光合性能和渗透调节能力的影响. 杂粮作物, 2008, 28(5): 312–315.

Shi L R, Cao Y S, Guo P. Effect of salt stress on the photosynthetic properties and osmotic adjustment ability in Vicia faba L. Rain Fed Crops, 2008, 28(5): 312–315 (in Chinese with English abstract).

[21] 王耘, 金潇潇, 赵辉, 郝振萍. 盐胁迫对鲜食蚕豆幼苗光合特性及抗氧化酶活性的影响. 北方园艺, 2017, (20): 25–30.

Wang Y, Jin X X, Zhao H, Hao Z P. Effect of salt stress on photosynthesis and antioxidant enzyme activities of Vicia faba L. Seedling. North Hortic, 2017, (20): 25–30 (in Chinese with English abstract).

[22] 孙晓东, 王兰, 李爱玲. 盐胁迫对蚕豆幼苗生长发育的影响. 陕西农业科学, 2017, 63(7): 19–23.

Sun X D, Wang L, Li A L. Effects of salt stress on growth and development of faba bean seedlings. Shaanxi J Agric Sci, 2017, 63(7): 19–23(in Chinese).

[23] 衡通, 艾鹏睿, 马英杰, 刘长江, 吴淼, 刘振月. 不同土壤盐分胁迫对棉花氮素利用效率及生长的影响. 新疆农业大学学报, 2023, 46: 333–340.

Heng T, Ai P R, Ma Y J, Liu C J, Wu M, Liu Z Y. Effects of different soil salinity stress on nitrogen use efficiency and cotton growth. J Xinjiang Agric Univ, 2023, 46: 333–340 (in Chinese with English abstract).

[24] 谭兵兵, 种培芳, 刘行行, 刘泽华. 外源NaHS对盐胁迫下红砂根系形态及碳氮磷含量的影响. 草地学报, 2024, 32(1): 139–147.

Tan B B, Chong P F, Liu H H, Liu Z H. Effects of exogenous NaHS on root morphology and carbon, nitrogen and phosphorus stoichiometry of

Reaumuria Soongorica under salt stress. Acta Agrest Sin, 2024, 32(1): 139–14 (in Chinese with English abstract).

[25] Ashraf M, Bashir A. Salt stress induced changes in some organic metabolites and ionic relations in nodules and other plant parts of two crop legumes differing in salt tolerance. Flora, 2003, 198: 486–498．

[26] Semida W M, Taha r S, Abdelhamid M T. Foliar-applied α tocopherol enhances salt-tolerance in faba bean L. Plants grown under saline  

conditions. South Afr J Bot, 2014, 95: 24–31.

[27] 肖鑫辉, 李向华, 刘洋, 张应, 王克晶. 高盐碱胁迫下野生大豆体内离子积累的差异. 作物学报, 2011, 37: 1289–1300.

Xiao X H, Li X H, Liu Y, Zhang Y, Wang K J. Difference of ion accumulation in wild soybean (Glycine soja) under high saline-alkali stress. Acta Agron Sin, 2011, 37: 1289–1300 (in Chinese with English abstract).

[28] 沈一, 刘永惠, 陈志德, 颜伟, 栾玉柱. 花生幼苗期耐盐品种的筛选与评价. 花生学报, 2012, 41(1): 10–15．

Shen Y, Liu Y H, Chen Z D, Yan W, Luan Y Z. Aion and evaluation of peanut varieties based on seedlings salt tolerance. J Peanut Sci, 2012, 41(1): 10–15 (in Chinese with English abstract).

[29] 乔亚科, 李桂兰, 高书国, 毕艳娟, 尤丽娜, 石向付, 张怡. 昌黎沿海野生大豆分布及其耐盐性. 河北职业技术师范学院学报, 2001, 15(2): 9–13.

Qiao Y K, Li G L, Gao S G, Bi Y J, You L N, Shi X F, Zhang Y. Geographical distribution and salt-tolerance of wild soybean in inshore regions in Changli Hebei province. J Hebei Vocat Te Chnical Teach Coll, 2001, 15(2): 9–13 (in Chinese with English abstract).

[30] 叶旭君, 汪成宏, 王仁第, 钱一飞, 王兆骞. 浙东沿海台灾受咸地冬季作物的适应性. 浙江农业学报, 1998, 10(6): 298–301.

Ye X J, Wang C H, Wang R D, Qian Y F, Wang Z Q. Winter crop suitability in saline-alkali caused by typhoon-driven sea floods in eastern

coastal Area of Zhejiang province. Acta Agric Zhejiangensis, 1998, 10(6): 298–301(in Chinese with English abstract).

[1] NIE Bo-Tao, LIU De-Quan, CHEN Jian, CUI Zheng-Guo, HOU Yun-Long, CHEN Liang, QIU Hong-Mei, WANG Yue-Qiang. Analysis and comprehensive evaluation of agronomic and quality traits of spring soybean varieties in northern China [J]. Acta Agronomica Sinica, 2024, 50(9): 2248-2266.
[2] YAN Feng, DONG Yang, LI Qing-Quan, ZHAO Fu-Yang, HOU Xiao-Min, LIU Yang, LI Qing-Chao, ZHAO Lei, FAN Guo-Quan, LIU Kai. Comprehensively evaluation on cold tolerance of foxtail millet varieties at germination stage [J]. Acta Agronomica Sinica, 2024, 50(9): 2207-2218.
[3] LI Xiao-Fei, GAO Hua-Wei, GUANG Hui, SHI Yu-Xin, GU Yong-Zhe, QI Zhao-Ming, QIU Li-Juan. Identification and evaluation of atrazine tolerance of soybean germplasm resources at germination stage and screening of excellent germplasm [J]. Acta Agronomica Sinica, 2024, 50(7): 1699-1709.
[4] LI Hang, LIU Li, HUANG Qian, LIU Wen-Hao, SI Ai-Jun, KONG Xian-Hui, WANG Xu-Wen, ZHAO Fu-Xiang, MEI Yong-Jun, YU Yu. Identification and screening of salt tolerance of cotton germplasm resources at germination stage [J]. Acta Agronomica Sinica, 2024, 50(5): 1147-1157.
[5] ZHANG Jing, GAO Wen-Bo, YAN Lin, ZHANG Zong-Wen, ZHOU Hai-Tao, WU Bin. Identification and evaluation of salt-alkali tolerance and screening of salt-alkali tolerant germplasm of oat (Avena sativa L.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1551-1561.
[6] SUN Xian-Jun, JIANG Qi-Yan, HU Zheng, LI Hong-Bo, PANG Bin-Shuang, ZHANG Feng-Ting, ZHANG Sheng-Quan, ZHANG Hui. Identification and evaluation of wheat germplasm resources at seedling stage [J]. Acta Agronomica Sinica, 2023, 49(4): 1132-1139.
[7] MENG Yu, TIAN Wen-Zhong, WEN Peng-Fei, DING Zhi-Qiang, ZHANG Xue-Pin, HE Li, DUAN Jian-Zhao, LIU Wan-Dai, GUO Tian-Cai, FENG Wei. Comprehensive evaluation of drought resistance of wheat varieties based on synergy of different developmental stages [J]. Acta Agronomica Sinica, 2023, 49(2): 570-582.
[8] LI Ji-Jun, CHEN Ya-Hui, WANG Yi-Jin, ZHOU Zhi-Hua, GUO Zi-Yue, ZHANG Jian, TU Jin-Xing, YAO Xuan, GUO Liang. Evaluation of field waterlogging tolerance and selection of waterlogging-resistant germplasm resources of Brassica napus L. [J]. Acta Agronomica Sinica, 2023, 49(12): 3162-3175.
[9] ZHU Ling-Xiao, SONG Shi-Jia, LI Hao-Ran, SUN Hong-Chun, ZHANG Yong-Jiang, BAI Zhi-Ying, ZHANG Ke, LI An-Chang, LIU Lian-Tao, LI Cun-Dong. Screening of low nitrogen tolerant cultivars based on low nitrogen tolerance comprehensive index at seeding stage in cotton [J]. Acta Agronomica Sinica, 2022, 48(7): 1800-1812.
[10] HU Liang-Liang, WANG Su-Hua, WANG Li-Xia, CHENG Xu-Zhen, CHEN Hong-Lin. Identification of salt tolerance and screening of salt tolerant germplasm of mungbean (Vigna radiate L.) at seedling stage [J]. Acta Agronomica Sinica, 2022, 48(2): 367-379.
[11] ZHU Ya-Di, WANG Hui-Qin, WANG Hong-Zhang, REN Hao, LYU Jian-Hua, ZHAO Bin, ZHANG Ji-Wang, REN Bai-Zhao, YIN Fu-Wei, LIU Peng. Evaluation and identification index of heat tolerance in different summer maize varieties at V12 stage [J]. Acta Agronomica Sinica, 2022, 48(12): 3130-3143.
[12] SONG Li-Jun, NIE Xiao-Yu, HE Lei-Lei, KUAI Jie, YANG Hua, GUO An-Guo, HUANG Jun-Sheng, FU Ting-Dong, WANG Bo, ZHOU Guang-Sheng. Screening and comprehensive evaluation of shade tolerance of forage soybean varieties [J]. Acta Agronomica Sinica, 2021, 47(9): 1741-1752.
[13] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[14] YAN Cai-Xia, WANG Juan, ZHAO Xiao-Bo, SONG Xiu-Xia, JIANG Chang-Song, SUN Quan-Xi, YUAN Cui-Ling, ZHANG Hao, SHAN Shi-Hua. Identification and screening of saline-alkali tolerant peanut cultivars during whole growth stage [J]. Acta Agronomica Sinica, 2021, 47(3): 556-565.
[15] GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui. Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus [J]. Acta Agronomica Sinica, 2019, 45(9): 1416-1430.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd