Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1779-1784.doi: 10.3724/SP.J.1006.2011.01771

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

ZHOU Ming-Jing1,2,WEN Yong2,LI Shuang-Cheng1,2,LI Cheng-Bo2,ZHANG Man-Hua2,GAO Feng-Yan2,WANG Ling-Xia1,2,LI Ping1,2,*   

  1. 1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2 Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education / Sichuan Agricultural University, Ya’an 625014, China
  • Received:2011-01-25 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 李平, E-mail: liping6575@163.com

PDF

2134

Abstract: Floral organ development directly affects rice yield and quality. Here we report a rice floral mutant with increased stigmas, which was isolated from the progenies of the combination of C2/2480. Compared with the wild type rice, the mutant showed dwarf plant, small panicle and grain, curled flag leaf, narrow and opening spikelet, as well as later flowering. Most of florets were made up of normal lodicule, increased stigmas, more pistils, and fewer stamens. Segregation of wild type plants to mutant plants in three F2 population were accorded with the ratio of 3:1, which indicated that the mutant is controlled by a single recessive gene. With SSR (Simple Sequence Repeat) markers screening in a total of 92 F2 mutant individuals derived from the cross of ism/T116, the mutant gene was located between the molecular markers RM3183 and RM3827, at the distances of 2.2 cM and 12.0 cM, respectively, on chromosome 6, and co-segregated with RM11951, RM19953 and RM19961. ISM(t) is a new floral-organ identity gene located on chromosome 6 in rice.This result provided a foundation of map-based cloning and function analysis of ISM(t) gene.

Key words: Rice, Floral organ, Mutant, Genetic analysis, Genetic mapping

[1]Liu J(刘坚), Guo L-B(郭龙彪), Qian Q(钱前). Research progress on flower development genes in rice. China Rice (中国稻米),2007, (3): 8–9 (in Chinese with English abstract)
[2]Parkinson C L, Adams K L, Palmer J D. Multigene analyses identify the three earliest lineages of extant flowering plants.Curr Biol, 1999, 9: 1485–1488
[3]Gao J-H(高晶涵), Li Q-Y(李清昀). Molecular mechanism controlling floral organ development in plant. Bull Biol (生物学通报), 2008, 43(2): 19–21 (in Chinese with English abstract)
[4]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353:31–37
[5]Xu Z-H(许智宏). Plant development and reproduction: advances and prospectives. Acta Bot Sin (植物学报), 1991, 41(9): 909–930(in Chinese with English Abstract)
[6]Theissen G, Saedler H. Floral quartets. Nature, 2001, 409:469–471
[7]Ferrario S, Immink R G H, Shchennikova A, Busscher-Lange J,Angenent G C. The MADS-box gene FBP2 is required for SEPALLATA function in petunia. Plant Cell, 2003, 15: 914–925
[8]Angenent G C, Franken J, Busscher M, van Dijken A, van Went 1784 作 物 学 报 第 37 卷 L, Dons H, van Tunen A J. A novel class of MADS-box genes is involved in ovule development in petunia. Plant Cell, 1994, 7:1569–1582
[9]Colombo L, Franken J, Koetje E, van Dijken A, van Went L,Dons H, van Tunen A J. The petunia MADS-box gene FBP11 determine ovule identity. Plant Cell, 1995, 7: 1859–1868
[10]Zhang X-Q(张向前), Zou J-S(邹金松), Zhu H-T(朱海涛), Li X-Y(李晓燕), Zeng R-Z(曾瑞珍). Genetic analysis and gene mapping of an early flowering and multi-ovary mutant in rice (Oryza sativa L.). Hereditas (Beijing) (遗传), 2008, 30(10):1349–1355 (in Chinese with English abstract)
[11]Hua Z-M(华志明). Progress of molecular development research in rice. Chin J Rice Sci (中国水稻科学), 2000, 14(4): 256–260(in Chinese with English abstract)
[12]Luo Q(罗琼), Zhu L-H(朱立煌). Progress of molecular floral development research in rice. Hereditas(Beijing) (遗传), 2002,24(1): 87–93 (in Chinese with English abstract)
[13]Li A-H(李爱宏), Wu R(武茹), Zhang Y-F(张亚芳), Tang W(汤雯), Wu C-Y(吴昌银), Pan X-B(潘学彪). Morphogenesis, anatomical observation and primary genetic analysis of a multi-glume floral organ mutant in rice. Chin J Rice Sci (中国水稻科学),2006, 20(4): 348–354 (in Chinese with English abstract)
[14]Li Y-F(李云峰), Luo H-F(罗洪发), Yang Z-L(杨正林), Zhong B-Q(钟秉强), He G-H(何光华). Genetic analysis on pistilliod-stamen mutant in rice. Chin J Rice Sci (中国水稻科学),2004, 18(6): 499–502 (in Chinese with English abstract)
[15]Wang B(王彬), Wu X-J(吴先军), Xie Z-H(谢兆辉). Study on the ABC model for flower development. Agric Biotechnol Sci (农业生物技术学报), 2003, 10(5): 78–82 (in Chinese with English abstract)
[16]Feng M, Fu D Z, Liang H X, Lu A. Floral morphogenesis of Aquilegia L. (Ranunculaceae). Acta Bot Sin, 1995, 37: 791–794
[17]Mizukami Y, Ma H. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell, 1992, 71: 119–131
[18]Zhu Q H, Ramm K, Shivakkumar R, Dennis E S, Upadhyaya N M. The ANTHER INDEHISCENCE1 gene encoding a single MYB domain protein is involved in anther development in rice.Plant Physiol, 2004, 135: 1514–1525
[19]Park J J, Jin P, Yoon J, Yang J I, Jeong H, Ranathunge K, Schreiber L, Franke R, Lee I J, An G. A mutation in WILTED DWARF and LETHAl 1 (WDL1) causes abnormal cuticle formation and rapid water loss in rice. Plant Mol Biol, 2010, 74: 91–103
[20]Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano H Y. The gene FLORAL ORGAN NUMBER1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development, 2004, 131:5649–5657
[21]Kalika P, Sriram P, Usha V H. OsMADS1, a rice MADS-box factor,controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs. Plant J, 2005, 43: 915–928
[22]Lee S Y, Kim J, Han J J, Han M J, An G. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/ AGL20) ortholog in rice. Plant J, 2004, 38: 754–764
[23]Nonomura K I, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H, Kurata N. The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice. Plant Cell, 2003, 15:1728–1739
[24]Luo H, Lee J Y, Hu Q, Kimberly N V, Timothy K, Eitas, Colin L, Albert P, Kausch, Joel M, Chandlee, Thomas K, Hodges R T S. A rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Mol Biol, 2003, 62: 397–408
[25]Lee D Y, Lee J, Moon S, Park Soung Y, An G. The rice heterochronic gene SUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meristem. Plant J, 2006, 49:64–78
[26]Li N, Zhang D D, Liu H S, Yin C H, Li X X, Liang W Q, Yuan Z,Xu B, Chu H W, Wang J, Wen T Q, Huang H, Luo D, Ma H,Zhang D B. The rice TAPETUM DEGENERATION RETARDATION gene is required for tapetum degradation and anther development.Plant Cell, 2006, 18: 2999–3014
[27]Jung K H, Han M J, Lee Y S, Kim Y W, Hwang I, Kim M J, Kim Y K, Nahm B H, An G. Rice UNDEVELOPED TAPETUML is a major regulator of early tapetum development. Plant Cell, 2005,17: 2705–2722
[28]Jang S, Hur J, Kim S J, Han M J, Kim S R, An G. Ectopic expression of OsYAB1 causes extra stamens and carpels in rice. Plant Mol Biol, 2004, 56: 133–143
[1] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[2] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[3] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[4] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[5] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[6] Shi Shao-Jie, Liu Kai, Chen Zi-Yi, Wang Hui-Ying, Li San-He, Zhou Lei, You Ai-Qing. Cloning and functional analysis of the dwarf and multi-tiller gene DMT1 in rice [J]. Acta Agronomica Sinica, 2026, 52(4): 1022-1034.
[7] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[8] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[9] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[10] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[11] Jing Xiu-Qing, Cai Yong-Duo, Deng Ning, Zhao Xiao-Dong, Zhai Fei-Hong, Zeng Qun. Identification and expression pattern analysis of RopGEF family genes in Chenopodium quinoa [J]. Acta Agronomica Sinica, 2026, 52(1): 28-43.
[12] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[13] Liu Di, Li Rui-Yuan, Shi Mao-Zhu, Li Hong-You, Chen Qing-Fu, Shi Tao-Xiong. Phenotypic characterization and transcriptomic analysis of the semi-dwarf mutant sd3 in Tartary buckwheat [J]. Acta Agronomica Sinica, 2026, 52(1): 316-328.
[14] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[15] GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng. Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive [J]. Acta Agronomica Sinica, 2025, 51(9): 2433-2453.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd