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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1779-1784.doi: 10.3724/SP.J.1006.2011.01771

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

ZHOU Ming-Jing1,2,WEN Yong2,LI Shuang-Cheng1,2,LI Cheng-Bo2,ZHANG Man-Hua2,GAO Feng-Yan2,WANG Ling-Xia1,2,LI Ping1,2,*   

  1. 1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2 Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education / Sichuan Agricultural University, Ya’an 625014, China
  • Received:2011-01-25 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 李平, E-mail: liping6575@163.com

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Abstract: Floral organ development directly affects rice yield and quality. Here we report a rice floral mutant with increased stigmas, which was isolated from the progenies of the combination of C2/2480. Compared with the wild type rice, the mutant showed dwarf plant, small panicle and grain, curled flag leaf, narrow and opening spikelet, as well as later flowering. Most of florets were made up of normal lodicule, increased stigmas, more pistils, and fewer stamens. Segregation of wild type plants to mutant plants in three F2 population were accorded with the ratio of 3:1, which indicated that the mutant is controlled by a single recessive gene. With SSR (Simple Sequence Repeat) markers screening in a total of 92 F2 mutant individuals derived from the cross of ism/T116, the mutant gene was located between the molecular markers RM3183 and RM3827, at the distances of 2.2 cM and 12.0 cM, respectively, on chromosome 6, and co-segregated with RM11951, RM19953 and RM19961. ISM(t) is a new floral-organ identity gene located on chromosome 6 in rice.This result provided a foundation of map-based cloning and function analysis of ISM(t) gene.

Key words: Rice, Floral organ, Mutant, Genetic analysis, Genetic mapping

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