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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1771-1778.doi: 10.3724/SP.J.1006.2011.01771

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Function Analysis of the Gene OsASIE1 Responding to Abiotic Stresses in Rice

WU Hui-Min,HUANG Li-Yu,PAN Ya-Jiao,JIN Peng,FU Bin-Ying*   

  1. Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-03-14 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 傅彬英, E-mail: fuby@caas.net.cn, Tel: 010-82106698 E-mail:wuhuimin86@126.com

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Abstract: AP2/EREBP transcription factors play an important role in plant development, hormone response, biotic and abiotic stress responses. We identified that OsASIE1, a member of EREBP subfamily of AP2/EREBP transcription factor family in rice, was involved in abiotic stress response. Expression of OsASIE1 wasinduced by drought and salt stresses, and over-expression of OsASIE1 in the transgenic rice plant could improve its tolerance to salt stress.Further electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of OsASIE1 protein could bind both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) in vitro. All these results implicated that OsASIE1 might be involved in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding.

Key words: Rice, AP2/EREBP transcription factor, EMSA, Salt tolerance, Over-expression

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