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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1771-1778.doi: 10.3724/SP.J.1006.2011.01771

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Function Analysis of the Gene OsASIE1 Responding to Abiotic Stresses in Rice

WU Hui-Min,HUANG Li-Yu,PAN Ya-Jiao,JIN Peng,FU Bin-Ying*   

  1. Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-03-14 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 傅彬英, E-mail: fuby@caas.net.cn, Tel: 010-82106698 E-mail:wuhuimin86@126.com

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Abstract: AP2/EREBP transcription factors play an important role in plant development, hormone response, biotic and abiotic stress responses. We identified that OsASIE1, a member of EREBP subfamily of AP2/EREBP transcription factor family in rice, was involved in abiotic stress response. Expression of OsASIE1 wasinduced by drought and salt stresses, and over-expression of OsASIE1 in the transgenic rice plant could improve its tolerance to salt stress.Further electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of OsASIE1 protein could bind both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) in vitro. All these results implicated that OsASIE1 might be involved in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding.

Key words: Rice, AP2/EREBP transcription factor, EMSA, Salt tolerance, Over-expression

[1]Nakashima K, Ito Y, Yamaguchi-Shinozaki K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol, 2009, 149: 88–95
[2]Hussain S S, Kayani M A, Amjad M. Transcription factors as tools to engineer enhanced drought stress tolerance in plants. Biotechnol Prog, 2011, 27: 297–306
[3]Sakuma Y, Liu Q, Joseph G. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREB, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophy Res Commun, 2002, 290: 998–1009
[4]Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol, 2006, 140: 411–432
[5]Gutterson N, Reuber T L. Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol, 2004, 7: 465–471
[6]Ohme-Takagi M, Shinshi H. Ethylene-inducible DNA binding proteins, that interact with an ethylene-responsive element. Plant Cell, 1995, 7: 173–182
[7]Gu Y Q, Wildermuth M C, Chakravarthy S. Tomato transcription factors Pti4, Pti5 and Pti6 activate defense responses when expressed in Arabidopsis. Plant Cell, 2002, 14: 817–831
[8]Zhou J M, Tang X Y, Martin G B. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J, 1997, 16: 3207–3218
[9]Hu Y B, Zhao L F, Chong K, Wang T. Overexpression of OsERF1, a novel rice ERF gene, up-regulates ethylene-responsive genes expression besides affects growth and development in Arabidopsis. Plant Physiol, 2008, 165: 1717–1725
[10]Xu K N, Xu X, Fukao T, Fukao P, Maghirang-Rodriguez R, Heuer S, Ismail A M, Bailey-Serres J, Ronald1 P C, Mackill D J. Sub1A is an ethylene response factor gene that confers submergence tolerance to rice. Nature, 2006, 442: 705–708
[11]Hattori Y, Nagai K, Nagai S. The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature, 2009, 460: 1026–1031
[12]Oh S J, Kim Y S, Kwon C W, Park H K, Jeong J S, Kim J K. Overexpression of the transcription factor AP37 in rice improves grain yield under drought conditions. Plant Physiol, 2009, 150: 1368–1379
[13]Yamaguchi-Shinozakiaib K, Shinozaki K A. Nove1 cis-acting element in an Arabidopsis genes involved in responsiveness to drought, low temperature, or high-salt stress. Plant Cell, 1996, 6: 251–264
[14]Baker S S, Wilhelm K S, Thomashow M F. The 5'-region of Arabidopsis thaliana corl5a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Mol Biol, 1994, 24: 701–713
[15]Stockinger E J, Gilmour S J, Thomashow M F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA, 1997, 94: 1035–1040
[16]Yamaguchi-Shinozaki K, Shinozaki K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol, 2006, 57: 781–803
[17]Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubouzet E G, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J, 2003, 33: 751–763
[18]Chen J Q, Meng X P, Zhang Y, Xia M, Wang X P. Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnol Lett, 2008, 30 (12): 2191–2198
[19]Zhang M(张梅), Liu W(刘炜), Bi Y-P(毕玉平). Dehydration-responsive element-binding (DREB) transcription factor in plants and its role during abiotic stresses. Hereditas (Beijing)(遗传), 2009, 31(3): 236–244 (in Chinese)
[20]Sun S, Yu J P, Chen F, Zhao T J, Fang X H, Li Y Q, Sui S F. TINY, a dehydration-responsive element (DRE)-binding protein-like transcription factor connecting the DRE- and ethylene-responsive element-mediated signaling pathways in Arabidopsis. Biol Chem, 2006, 283: 6261–6271
[21]Wei G, Pan Y, Lei J, Zhu Y X. Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana. Biochem Mol Biol, 2005, 38: 440–446
[22]Liua Y, Zhao T J, Liu J M, Liue W Q, Liua Q, Yan Y B, Zhou H M. The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box. FEBS Lett, 2006, 580: 1303–1308
[23]Yang S, Yang S C, Liu X, Liu Y, Liu L, Wang X, Hao D Y. Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference. FEBS J, 2009, 276: 7177–7186
[24]Jin P(靳鹏), Huang L-Y(黄立钰), Wang D(王迪), Wu H-M(吴慧敏), Zhu L-H(朱苓华), Fu B-Y(傅彬英). Expression profiling of rice AP2/EREBP Genes responsive to abiotic stresses. Sci Agric Sin (中国农业科学), 2009, 42(11): 3765–3773 (in Chinese with English abstract)
[25]Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol, 1987, 4: 406–425
[26]Chen H(陈惠), Zhao Y(赵原), Chong K(种康). Improved high-efficiency system for rice transformation using mature embryo-derived calli. Chin Bull Bot (植物学通报), 2008, 25(3): 322–331 (in Chinese with English abstract)
[27]Liu Qi, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low- temperature- responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998, 10: 1391–1406
[28]Sharoni A M, Nuruzzaman M, Satoh K, Shimizu T, Kondoh1 H, Sasaya T, Choi I R, Omura T, Kikuchi S. Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant Cell Physiol, 2011, 52: 344–360
[29]Qin F, Sakuma Y, Tran L S P, Maruyama K, Kidokoro S, Fujita Y, Fujita M, Umezawa T, Sawano Y, Miyazono K I, Tanokura M, Shinozaki K, Yamaguchi-Shinozakia K. Arabidopsis DREB2A interacting proteins function as RING E3 ligases and negatively regulate plant drought stress–responsive gene expression. Plant Cell, 2008, 20: 1693–1707
[30]Kidokoro S, Maruyama K, Nakashima K, Imura Y, Narusaka Y , Shinwari Z K, Osakabe Y, Fujita Y, Mizoi J, Shinozaki K, Yamaguchi-Shinozak K. The phytochrome-interacting factor PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis. Plant Physiol, 2009, 151: 2046–57
[31]Zhu H-C(朱厚础). Experiment Guide of Protein Purification and Identification (蛋白质纯化与鉴定实验指南). Beijing: Science Press, 1999. pp 158–159 (in Chinese)
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