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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 256-263.doi: 10.3724/SP.J.1006.2012.00256

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping and Meta-Analysis of QTLs for Leaf Traits in Soybean

SHI Xiang-Lin1,SUN Ya-Nan1,WANG Jia-Lin1,LIU Chun-Yan1,2,CHEN Qing-Shan1,*,HU Guo-Hua2,3,*   

  1. 1 Graduate College of Northeast Agricultural University, Harbin 150030, China; 2 The Crop Research and Breeding Center of Land-Reclamation, Harbin 150090, China; 3 The National Research Center of Soybean Engineering and Technology, Harbin 150050, China
  • Received:2011-04-25 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-01
  • Contact: 胡国华, E-mail: Hugh757@vip.163.com, 0451-55199475; 陈庆山, E-mail: qshchen@126.com, 0451-55191945

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Abstract: Leaf length, width and leafstalk length affect the photosynthetic capability of plant and so increasing photosynthetic rate per unit leaf area may improve seed yield in soybean. In this study, we analyzed QTLs data of soybean leaf length, width and leafstalk length from 2006 to 2010with a F2:14–F2:18 of recombination inbred lines (RIL) population derived from a cross between Charleston and Dongnong 594 by mixed linear model approach. Eight QTLs for leaf length(LL) were mapped on the chromosomes Gm01, Gm02, Gm05, Gm11, Gm18 by software WinQTLCartographer Ver. 2.5, nine QTLs were identi?ed for leaf width (LW) on the chromosomes Gm01, Gm03, Gm05, Gm06, Gm11, Gm12, Gm16; eight QTLs were identi?ed for leafstalk length (LSL) on Gm01, Gm03, Gm05, Gm06, Gm11, Gm17, Gm18. qLL5a, qLL5b, qLL1a, and qLL18 for LL, qLW5a, qLW11a, qLW11b, and qLW12 for LW, and qLSL11b for LSL were identi?ed in more than two years. Furthermore, not only 72 QTLs of leaf traits that have been mapped in many different populations and environments were collected but also QTL mapped by WinQTLCartographer Ver. 2.5 were projected and integrated in the reference map with the software BioMercator2.1. In total, the consensus QTLs of five for leaf length and four for leaf width were obtained in soybean, respectively. The minimum confidence interval of leaf length was shrunk to 5.66 cM. These results would provide a basis for fine mapping QTL and cloning genes in soybean.

Key words: Soybean [Glycine max (L.) Merr.], Leaf traits, QTL mapping, Meta-analysis

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