Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 256-263.doi: 10.3724/SP.J.1006.2012.00256

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping and Meta-Analysis of QTLs for Leaf Traits in Soybean

SHI Xiang-Lin1,SUN Ya-Nan1,WANG Jia-Lin1,LIU Chun-Yan1,2,CHEN Qing-Shan1,*,HU Guo-Hua2,3,*   

  1. 1 Graduate College of Northeast Agricultural University, Harbin 150030, China; 2 The Crop Research and Breeding Center of Land-Reclamation, Harbin 150090, China; 3 The National Research Center of Soybean Engineering and Technology, Harbin 150050, China
  • Received:2011-04-25 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-01
  • Contact: 胡国华, E-mail: Hugh757@vip.163.com, 0451-55199475; 陈庆山, E-mail: qshchen@126.com, 0451-55191945

PDF

1545

Cited

4

Abstract: Leaf length, width and leafstalk length affect the photosynthetic capability of plant and so increasing photosynthetic rate per unit leaf area may improve seed yield in soybean. In this study, we analyzed QTLs data of soybean leaf length, width and leafstalk length from 2006 to 2010with a F2:14–F2:18 of recombination inbred lines (RIL) population derived from a cross between Charleston and Dongnong 594 by mixed linear model approach. Eight QTLs for leaf length(LL) were mapped on the chromosomes Gm01, Gm02, Gm05, Gm11, Gm18 by software WinQTLCartographer Ver. 2.5, nine QTLs were identi?ed for leaf width (LW) on the chromosomes Gm01, Gm03, Gm05, Gm06, Gm11, Gm12, Gm16; eight QTLs were identi?ed for leafstalk length (LSL) on Gm01, Gm03, Gm05, Gm06, Gm11, Gm17, Gm18. qLL5a, qLL5b, qLL1a, and qLL18 for LL, qLW5a, qLW11a, qLW11b, and qLW12 for LW, and qLSL11b for LSL were identi?ed in more than two years. Furthermore, not only 72 QTLs of leaf traits that have been mapped in many different populations and environments were collected but also QTL mapped by WinQTLCartographer Ver. 2.5 were projected and integrated in the reference map with the software BioMercator2.1. In total, the consensus QTLs of five for leaf length and four for leaf width were obtained in soybean, respectively. The minimum confidence interval of leaf length was shrunk to 5.66 cM. These results would provide a basis for fine mapping QTL and cloning genes in soybean.

Key words: Soybean [Glycine max (L.) Merr.], Leaf traits, QTL mapping, Meta-analysis

[1]Kokubun M. Soybean cultivar difference in leaf photo-synthetic rate and its relation to seed yield. Jpn J Crop Sci, 1988, 57(4): 743–748

[2]Hu M-X(胡明祥), Li K-M(李开明), Tian P-Z(田佩占), Yu D-Y(于德洋). Breeding for high yield and plant type of soybean. J Jilin Agric Sci (吉林农业科学), 1980, (3): 1–14 (in Chinese)

[3]Fu Y-H(傅艳华), Sun S-X(孙淑贤), Peng B(彭宝). Effect of soybean yield and nitrogen uptake by cutting leaves. Crops (作物杂志), 1997, (2): 27–28 (in Chinese)

[4]Thompson J A, R L Nelson, Schweitzer L E. Relationships among speci?c leaf weight, photosynthetic rate, and seed yield in soybean. Crop Sci, 1995, 35: 1575–1581

[5]Ellis R H, Asumada H, Qi A, Summer?eld R J. Effects of photoperiod and maturity genes on plant growth, partitioning radiation use efficiency, and yield in soyabean [Glycine max (L.) Merrill] ‘Clark’. Ann Bot, 2000, 85: 335–343

[6]Mansur L M, K G Lark, H Kross, Oliveira A. Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Theor Appl Genet, 1993, 86: 907–913

[7]Mansur L M, Orf J H, Chase K, Jarvik T, Cregan P B, Lark K G. Genetic mapping of agronomic traits using recombinant inbred lines of soybean. Crop Sci, 1996, 36: 1327–1336

[8]Keim P, Diers B W, Olson T C, Shoemaker R C. RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics, 1990, 126: 735–742

[9]Kim H K, Kang S T, Suh D Y. Analysis of quantitative trait loci associated with leaflet types in two recombinant inbred lines of soybean. Plant Breed, 2005, 124: 582–589

[10]Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122−128

[11]Glass G V. Primary, secondary, and meta-analysis of research. Educatl Res, 1976, 5: 3−8

[12]Goffinet B, Gerber S. Quantitative trait loci: a meta-analysis. Genetics, 2000, 155: 463−473

[13]Etzel C J, Guerra R. Meta-analysis of genetic-linkage analysis of quantitative-trait loci. Am J Human Genet, 2002, 71: 56−65

[14]Chardon F, Virlon B, Moreau L, Falque M, Joets J, Decousset L, Murigneux A, Charcosset A. Genetic architecture of flowering time in maize as inferred from quantitative trait loci meta-analysis and synteny conservation with the rice genome. Genetics, 2004, 168: 2169−2185

[15]Truntzler M, Barriere Y, Sawkins M C, Lespinasse D, Betran J, Charcosset A, Moreau L. Meta-analysis of QTL involved in silage quality of maize and comparison with the position of candidate genes. Theor Appl Genet, 2010, 121: 1465−1482

[16]Guo B, Sleper D A, Lu P, Shannon J G, Nguyen H T, Arelli P R. QTLs associated with resistance to soybean cyst nematode in soybean: meta-analysis of QTL location. Crop Sci, 2006, 46: 595−602

[17]Sun Y-N(孙亚男), Qi Z-M(齐照明), Shan D-P(单大鹏), Liu C-Y(刘春燕), Hu G-H(胡国华), Chen Q-S(陈庆山). Mapping and meta-analysis of height QTLs in soybean. Mol Plant Breed (分子植物育种), 2010, 4(8): 687−693 (in Chinese with English abstract)

[18]Wang X-Z(王贤智), Zhang X-J(张晓娟), Zhou R(周蓉), Sha A-H(沙爱华), Wu X-J(吴学军), Cai S-P(蔡淑平), Qiu D-Z(邱德珍), Zhou X-A(周新安). QTL analysis of seed and pod traits in soybean RIL population. Acta Agron Sin (作物学报), 2007, 33(3): 441−448 (in Chinese with English abstract)

[19]Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕), Wang W-Q(王伟权), Li W-B(李文滨). Construction and analysis of soybean genetic map using recombinant inbred line of Charleston×Dongnong 594. Sci Agric Sin (中国农业科学), 2005, 38(7): 1312−1316 (in Chinese with English abstract)

[20]Darvasi A, Weinreb A, Minke V, Weller J I, Soller M. Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics, 1993, 134: 943−951

[21]Darvasi A, Soller M. A simple method to calculate resolving power and confidence interval of QTL map location. Behavior Genet, 1997, 27: 125−132

[22]Orf J H, Chase K, Jarvik T, Mansur L M, Cregan P B, Adler F R, Lark K G. Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations. Crop Sci, 1999, 39: 1642−1651

[23]Wang Z(王珍). Construction of Soybean SSR Based Map and QTL Analysis Important Agronomic Traits. MS Dissertation of Guangxi University, 2004. pp 66−72 (in Chinese with English abstract)

[24]Zeng Z B. Precision mapping of quantitative trait loci. Genetics, 1994, 136: 1457−1468

[25]Kao C H, Zeng Z B, Robert D. T. Multiple interval mapping for quantitative trait loci. Genetics, 1999, 152: 1203−1216

[26]Jansen R C, Van Ooijien J M, Stam P, Lister C, Dean C. Genotype-by-environment interaction in genetic mapping of multiple quantitative trait loci. Theor Appl Genet, 1995, 91: 33−37
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[3] ZHANG Bo, PEI Rui-Qing, YANG Wei-Feng, ZHU Hai-Tao, LIU Gui-Fu, ZHANG Gui-Quan, WANG Shao-Kui. Mapping and identification QTLs controlling grain size in rice (Oryza sativa L.) by using single segment substitution lines derived from IAPAR9 [J]. Acta Agronomica Sinica, 2021, 47(8): 1472-1480.
[4] ZHOU Xin-Tong, GUO Qing-Qing, CHEN Xue, LI Jia-Na, WANG Rui. Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(4): 587-598.
[5] LI Shu-Yu, HUANG Yang, XIONG Jie, DING Ge, CHEN Lun-Lin, SONG Lai-Qiang. QTL mapping and candidate genes screening of earliness traits in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(4): 626-637.
[6] LUO Kai, XIE Chen, WANG Jin, WANG Tian, HE Shun, YONG Tai-Wen, YANG Wen-Yu. Effect of exogenous plant growth regulators on carbon-nitrogen metabolism and flower-pod abscission of relay strip intercropping soybean [J]. Acta Agronomica Sinica, 2021, 47(4): 752-760.
[7] SHEN Wen-Qiang, ZHAO Bing-Bing, YU Guo-Ling, LI Feng-Fei, ZHU Xiao-Yan, MA Fu-Ying, LI Yun-Feng, HE Guang-Hua, ZHAO Fang-Ming. Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits [J]. Acta Agronomica Sinica, 2021, 47(3): 451-461.
[8] LIAN Yun, WANG Jin-She, WEI He, LI Jin-Ying, GONG Gui-Ming, WANG Shu-Feng, ZHANG Jing-Peng, LI Mao-Lin, GUO Jian-Qiu, LU Wei-Quo. Distribution survey of soybean cyst nematode of new race X12 in Gujiao city, Shanxi province [J]. Acta Agronomica Sinica, 2021, 47(2): 237-244.
[9] QIN Xiao-Min, PAN Hao-Nan, XIAO Jing-Xiu, TANG Li, ZHENG Yi. Effects of maize and soybean intercropping on nodule growth, nitrogen fixation of soybean under low phosphorus condition [J]. Acta Agronomica Sinica, 2021, 47(11): 2268-2277.
[10] Dai-Ling LIU,Jun-Feng XIE,Qian-Rui HE,Si-Wei CHEN,Yue HU,Jia ZHOU,Yue-Hui SHE,Wei-Guo LIU,Wen-Yu YANG,Xiao-Ling WU. QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping [J]. Acta Agronomica Sinica, 2020, 46(3): 341-353.
[11] WU Hai-Tao, ZHANG Yong, SU Bo-Hong, Lamlom F Sobhi, QIU Li-Juan. Development of molecular markers and fine mapping of qBN-18 locus related to branch number in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2020, 46(11): 1667-1677.
[12] WANG Cun-Hu,LIU Dong,XU Rui-Neng,YANG Yong-Qing,LIAO Hong. Mapping of QTLs for leafstalk angle in soybean [J]. Acta Agronomica Sinica, 2020, 46(01): 9-19.
[13] YANG Xiao-Meng, LI Xia, PU Xiao-Ying, DU Juan, Muhammad Kazim Ali, YANG Jia-Zhen, ZENG Ya-Wen, YANG Tao. QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population [J]. Acta Agronomica Sinica, 2020, 46(01): 52-61.
[14] WANG Da-Chuan,WANG Hui,MA Fu-Ying,DU Jie,ZHANG Jia-Yu,XU Guang-Yi,HE Guang-Hua,LI Yun-Feng,LING Ying-Hua,ZHAO Fang-Ming. Identification of rice chromosome segment substitution Line Z747 with increased grain number and QTL mapping for related traits [J]. Acta Agronomica Sinica, 2020, 46(01): 140-146.
[15] Li-Juan WEI,Rui-Ying LIU,Li ZHANG,Zhi-You CHEN,Hong YANG,Qiang HUO,Jia-Na LI. Detection of stem height QTL and integration of the loci for plant height- related traits in B. napus [J]. Acta Agronomica Sinica, 2019, 45(6): 818-828.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd