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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 773-779.doi: 10.3724/SP.J.1006.2012.00773

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of SN1 Transgenic Wheat Plants with Enhanced Resistance to Rhizoctonia cerealis and Bipolaris sorokiniana

WANG Jin-Feng1,2,DU Li-Pu1,LI Zhao1,HUANG Su-Ping1,2,YE Xing-Guo1,FENG Dou2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Guangxi University, Nanning 530004, China
  • Received:2011-11-08 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn

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Abstract: SN1 is an anti-microbial peptide in potato, and can inhibit the growth of important phytopathogens. In this study, the transformation vector of SN1 gene expressing highly in monocot plants, pA25-SN1, was correctly constructed, in which SN1 gene was driven by maize ubiquitinpromoter. Four thousand embryo calli of Yangmai 18 were bombarded by the particle containing pA25-SN1. Among the regenerated 203 plants, 55 positive transgenic individuals were identified by PCR assay in the T0 generation with a transformation frequency of 1.38%. The transgenic wheat plants from T0 to T2 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR assays. The disease responses of these positive transgenic plants were also evaluated by inoculating with Rhizoctonia cerealisand Bipolaris sorokiniana. The results showed that SN1 gene was integrated into these transgenic lines, inherited from T0 to T2, and expressed in the wheat background. The transgenic wheat plants expressing SN1 displayed anenhanced resistance to R. cerealis and B. sorokiniana compared with the untransformed Yangmai 18, and the enhanced resistance was inheritable.

Key words: SN1 gene, Transgenic wheat, Rhizoctonia cerealis, Bipolaris sorokiniana, Resistance

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