Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 773-779.doi: 10.3724/SP.J.1006.2012.00773

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of SN1 Transgenic Wheat Plants with Enhanced Resistance to Rhizoctonia cerealis and Bipolaris sorokiniana

WANG Jin-Feng1,2,DU Li-Pu1,LI Zhao1,HUANG Su-Ping1,2,YE Xing-Guo1,FENG Dou2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Guangxi University, Nanning 530004, China
  • Received:2011-11-08 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn

PDF

2052

Cited

2

Abstract: SN1 is an anti-microbial peptide in potato, and can inhibit the growth of important phytopathogens. In this study, the transformation vector of SN1 gene expressing highly in monocot plants, pA25-SN1, was correctly constructed, in which SN1 gene was driven by maize ubiquitinpromoter. Four thousand embryo calli of Yangmai 18 were bombarded by the particle containing pA25-SN1. Among the regenerated 203 plants, 55 positive transgenic individuals were identified by PCR assay in the T0 generation with a transformation frequency of 1.38%. The transgenic wheat plants from T0 to T2 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR assays. The disease responses of these positive transgenic plants were also evaluated by inoculating with Rhizoctonia cerealisand Bipolaris sorokiniana. The results showed that SN1 gene was integrated into these transgenic lines, inherited from T0 to T2, and expressed in the wheat background. The transgenic wheat plants expressing SN1 displayed anenhanced resistance to R. cerealis and B. sorokiniana compared with the untransformed Yangmai 18, and the enhanced resistance was inheritable.

Key words: SN1 gene, Transgenic wheat, Rhizoctonia cerealis, Bipolaris sorokiniana, Resistance

[l]Chen Y-X(陈延熙), Tang W-H(唐文华), Zhang D-H(张敦华), Jian X-Y(简小鹰). A preliminary study on etiology of sharp eye-spot of wheat in China. Acta Phytophylacica Sin (植物保护学报), 1986, 13(1): 39–44 (in Chinese with English abstract)

[2]Lu Y(路妍), Zhang Z-Y(张增艳), Ren L-J(任丽娟), Liu B-Y(刘宝业), Liao Y(廖勇), Xu H-J(徐惠君), Du L-P(杜丽璞), Ma H-X(马鸿翔), Ren Z-L(任正隆), Jing J-X(井金学), Xin Z-Y(辛志勇). Molecular analyses on Rs-AFP2 transgenic wheat plants and their resistance to Rhizoctonia cerealis. Acta Agron Sin (作物学报), 2009, 35(4): 640–646 (in Chinese with English abstract)

[3]Kumar J, Schafer P, Hückelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan, S, Kogel K H. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Mol Plant Pathol, 2002, 3: 185–195

[4]Theis T, Stahl U. Antifungal proteins: targets, mechanisms and prospective applications. Cell Mol Life Sci, 2004, 61: 437–455

[5]Thevissen K, Terras F R G, Broekaert W F. Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl Environ Microbiol, 1999, 65: 5451–5458

[6]Turrini A,  Sbrana C, Pitto L, Castiglione M R, Giorgetti L, Briganti R, Bracci T, Evangelista M, Nuti M P, Giovannetti M. The antifungal Dm-AMP1 protein from Dahlia merckii expressed in Solanum melongena is released in root exudates and differentially affects pathogenic fungi and mycorrhizal symbiosis. New Phytol, 2004, 163: 393–403

[7]Segura A, Moreno M, Madueño F, Molina A, García-Olmedo F. Snakin-1, a peptide from potato that is active against plant pathogens. Mol Plant-Microbe Interact, 1999, 12: 16–23

[8]Jiang W(蒋雯), Liu X(刘欣), Zhang Z-Y(张增艳). Cloning and overexpression of defensin SN1 in Escherichia coli and its antifungal assay. J Plant Genet Resour (植物遗传资源学报), 2011, 12(2): 286–290 (in Chinese with English abstract)

[9]Alnasia N, Bazzini A, Hopp H E, Vazquez-Rovere C. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Plant Pathol, 2008, 9: 329–338

[10]Xu H-J(徐惠君), Pang J-L(庞俊兰), Ye X-G(叶兴国), Du L-P(杜丽璞), Li L-C(李连城), Xin Z-Y(辛志勇), Ma Y-Z(马有志), Chen J-P(陈剑平), Chen J(陈炯), Cheng S-H(程顺和), Wu H-Y(吴宏亚). Study on the gene transferring of Nib8 into wheat for its resistance to the Yellow mosaic virus by bombardment. Acta Agron Sin (作物学报), 2001, 27(6): 684–689 (in Chinese with English abstract)

[11]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325 (in Chinese with English abstract)

[12]Sharp P J, Kries M, Shewry P R, Gale M D. Location of amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75: 286–290

[13]Cai S-B(蔡士宾), Ren L-J(任丽娟), Yan W(颜伟), Wu J-Z(吴纪中), Chen H-G(陈怀谷), Wu X-Y(吴小有), Zhang X-Y(张仙义). Germplasm development and mapping of resistance to sharp eyespot (Rhizoctonia cerealis) in wheat. Sci Agric Sin (中国农业科学), 2006, 39(5): 928–934 (in Chinese with English abstract)

[14]Dong N, Liu X, Lu Y, Du L, Xu H, Liu H, Xin Z, Zhang Z. Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Func Integr Genomics, 2010, 10: 215–226

[15]Li Z, Zhou M, Zhang Z, Ren L, Du L, Zhang B, Xu H, Xin Z. Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Func Integr Genomics, 2011, 11: 63–70

[16]Jha S, Tank H G, Prasad B D, Chattoo B B. Expression of Dm-AMP1 in rice confers resistance to Magnaporthe oryzae and Rhizoctonia solani. Transgenic Res, 2009, 18: 59–69
[1] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2] Niu Li, Wang Yong-Sheng, Wang Chang-Jie, Zhang Hong, Meng Ya-Xiong, Li Bao-Chun, Yang Ke, Ma Xiao-Le, Yao Li-Rong, Si Er-Jing, Wang Hua-Jun, Wang Jun-Cheng. Identification and analysis of the NAC gene family in barley (Hordeum vulgare L.) and functional validation of HvNAC38 in salt tolerance [J]. Acta Agronomica Sinica, 2026, 52(3): 688-707.
[3] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[4] Lu Ya-Ni, Ding Chao-Jie, Zhang Yu, Du Xi-Jun, Qi Xue-Li, Hu Lin, Xu Wei-Gang. Identification and genome-wide association analysis of seedling-stage Fusarium crown rot resistance in 200 wheat cultivars from Henan province, China [J]. Acta Agronomica Sinica, 2026, 52(2): 363-375.
[5] Wang Yue-Sheng, Ge Dong-Dong, Cheng Lan-Fei, Chen Chun-Huan, Wang Chang-You, Liu Xin-Lun, Li Ting-Dong, Deng Ping-Chuan, Ji Wan-Quan, Zhao Ji-Xin. Molecular cytogenetic and disease resistance characterization of the wheat- Psathyrostachys huashanica disomic substitution line 16DH25-7 [J]. Acta Agronomica Sinica, 2026, 52(2): 433-445.
[6] Qi Qing-Song, Niu Xiang-Yu, Liu Bing-Ke, Kang Lu, Wang Chen, Feng De-Shun. Salt tolerance identification, screening and salt tolerance index evaluation of wheat-Thinopyrum intermedium radiation mutagenesis germplasm at germination and seedling stage [J]. Acta Agronomica Sinica, 2026, 52(2): 389-404.
[7] Su Ai-Guo, Xiao Sen-Lin, Yi Hong-Mei, Duan Sai-Ru, Wang Shuai-Shuai, Zhang Ru-Yang, Xing Jin-Feng, Li Chun-Hui, Sun Xuan, Xu Rui-Bin, Xu Tian-Jun, Li Zhi-Yong, Zhang Yong, Wang Rong-Huan, Song Wei, Zhao Jiu-Ran. Research progress and breeding application of resistance genetics to ear rot in maize [J]. Acta Agronomica Sinica, 2026, 52(1): 1-13.
[8] Dong Li-Hua, Dong Cheng-Yan, Li Zheng-Nan, Yu Jing, Ye Liang, Liu Fang, Tan Jing. Screening and identification of candidate resistance genes to gibberella ear rot caused by Fusarium graminearum in maize [J]. Acta Agronomica Sinica, 2026, 52(1): 131-147.
[9] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[10] ZHU Jin-Cheng, YANG Qiu-Hua, CHENG Li-Xiang, LI Wen-Li, SHI Ming-Ming, LI Hui-Xia, ZHANG Feng. Screening of potato germplasm for resistance to Meloidogyne incognita and analysis of related physiological responses [J]. Acta Agronomica Sinica, 2025, 51(9): 2307-2317.
[11] LI Yun-Xiang, GUO Qian-Qian, HOU Wan-Wei, ZHANG Xiao-Juan. Genome-wide association analysis of drought resistance traits in wheat seedlings introduced from ICARDA [J]. Acta Agronomica Sinica, 2025, 51(9): 2387-2398.
[12] GAO Meng-Juan, ZHAO He-Ying, CHEN Jia-Hui, CHEN Xiao-Qian, NIU Meng-Kang, QIAN Qi-Run, CUI Lu-Fei, XING Jiang-Min, YIN Qing-Miao, GUO Wen, ZHANG Ning, SUN Cong-Wei, YANG Xia, PEI Dan, JIA Ao-Lin, CHEN Feng, YU Xiao-Dong, REN Yan. Mapping and identification of a novel sharp eyespot resistance locus Qse.hnau-5AS and its candidate genes in wheat [J]. Acta Agronomica Sinica, 2025, 51(8): 2240-2250.
[13] YIN Yu-Meng, WANG Yan-Nan, KANG Zhi-He, QIAO Shou-Chen, BIAN Qian-Qian, LI Ya-Wei, CAO Guo-Zheng, ZHAO Guo-Rui, XU Dan-Dan, YANG Yu-Feng. Cloning and functional analysis of glutathione S-transferase gene IbGSTU7 in sweetpotato [J]. Acta Agronomica Sinica, 2025, 51(7): 1736-1746.
[14] YANG Shuang, BAI Lei, GUO Hua-Chun, MIAO Ya-Sheng, LI Jun. Morphological characteristics, types, and developmental process of potato leaf trichomes [J]. Acta Agronomica Sinica, 2025, 51(6): 1582-1598.
[15] SHEN Wen-Jie, CHEN Qing-Qing, HU Yi-Qun, ZHANG Ai-Fang, ZHANG Man-Yu. Study on the sensitivity of various physiological races of Magnaporthe oryzae to isoprothiolane in Anhui province, China [J]. Acta Agronomica Sinica, 2025, 51(5): 1338-1346.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd