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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 780-790.doi: 10.3724/SP.J.1006.2012.00780

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Differential Expressed Proteins Responding to Phosphorus Starvation Based on Proteomic Analysis in Roots of Wheat (Triticum aestivum L.)

FENG Wan-Jun1,2,LI Zhen-Xing1,2,GUO Bao-Jian1,2,PENG Hui-Ru1,2,YAO Ying-Yin1,2,NI Zhong-Fu1,2,*,SUN Qi-Xin1,2,*   

  1. 1 Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; 2 National Plant Gene Research Centre (Beijing), Beijing 100193, China
  • Received:2011-09-05 Revised:2011-12-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 倪中福, E-mail: wheat3392@cau.edu.cn; 孙其信, E-mail: qxsun@cau.edu.cn

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Abstract: Growth inhibition caused by phosphorus (P) deficiency is a serious problem for crop production. Plants can respond defensively to this stress by modifying their metabolic pathways and root morphologies through changes of quantity of low-Pi responding genes. To better understand the adaptation mechanisms of wheat to Pi deficiency conditions, a comparative proteome analysis was conducted in this study using wheat root samples treated 7 days without (–P) and with phosphorus (+P). Among 1 144 protein spots reproducibly detected, eighty seven of which (7.6%) were differentially expressed, including those present in treatments of only in –P or +P, and up- or down-regulated in –P. Moreover, 39 of the differentially expressed proteins were revealed to be involved in various biological processes such as metabolism, cell growth and division, transcription and translation, disease and defense, signal transduction, acting as transposable elements and unclassified proteins. Taken together, our results showed that wheat responds to the Pi starvation stress through an array of changes in metabolic states of cells and genes expression, which results in the maintenance of a relative P homeostasis in plants.

Key words: Phosphorus nutrition, Proteomic analysis, Root, Triticum aestivum L.

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