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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 773-779.doi: 10.3724/SP.J.1006.2012.00773

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of SN1 Transgenic Wheat Plants with Enhanced Resistance to Rhizoctonia cerealis and Bipolaris sorokiniana

WANG Jin-Feng1,2,DU Li-Pu1,LI Zhao1,HUANG Su-Ping1,2,YE Xing-Guo1,FENG Dou2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Guangxi University, Nanning 530004, China
  • Received:2011-11-08 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn

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Abstract: SN1 is an anti-microbial peptide in potato, and can inhibit the growth of important phytopathogens. In this study, the transformation vector of SN1 gene expressing highly in monocot plants, pA25-SN1, was correctly constructed, in which SN1 gene was driven by maize ubiquitinpromoter. Four thousand embryo calli of Yangmai 18 were bombarded by the particle containing pA25-SN1. Among the regenerated 203 plants, 55 positive transgenic individuals were identified by PCR assay in the T0 generation with a transformation frequency of 1.38%. The transgenic wheat plants from T0 to T2 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR assays. The disease responses of these positive transgenic plants were also evaluated by inoculating with Rhizoctonia cerealisand Bipolaris sorokiniana. The results showed that SN1 gene was integrated into these transgenic lines, inherited from T0 to T2, and expressed in the wheat background. The transgenic wheat plants expressing SN1 displayed anenhanced resistance to R. cerealis and B. sorokiniana compared with the untransformed Yangmai 18, and the enhanced resistance was inheritable.

Key words: SN1 gene, Transgenic wheat, Rhizoctonia cerealis, Bipolaris sorokiniana, Resistance

[l]Chen Y-X(陈延熙), Tang W-H(唐文华), Zhang D-H(张敦华), Jian X-Y(简小鹰). A preliminary study on etiology of sharp eye-spot of wheat in China. Acta Phytophylacica Sin (植物保护学报), 1986, 13(1): 39–44 (in Chinese with English abstract)

[2]Lu Y(路妍), Zhang Z-Y(张增艳), Ren L-J(任丽娟), Liu B-Y(刘宝业), Liao Y(廖勇), Xu H-J(徐惠君), Du L-P(杜丽璞), Ma H-X(马鸿翔), Ren Z-L(任正隆), Jing J-X(井金学), Xin Z-Y(辛志勇). Molecular analyses on Rs-AFP2 transgenic wheat plants and their resistance to Rhizoctonia cerealis. Acta Agron Sin (作物学报), 2009, 35(4): 640–646 (in Chinese with English abstract)

[3]Kumar J, Schafer P, Hückelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan, S, Kogel K H. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Mol Plant Pathol, 2002, 3: 185–195

[4]Theis T, Stahl U. Antifungal proteins: targets, mechanisms and prospective applications. Cell Mol Life Sci, 2004, 61: 437–455

[5]Thevissen K, Terras F R G, Broekaert W F. Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl Environ Microbiol, 1999, 65: 5451–5458

[6]Turrini A,  Sbrana C, Pitto L, Castiglione M R, Giorgetti L, Briganti R, Bracci T, Evangelista M, Nuti M P, Giovannetti M. The antifungal Dm-AMP1 protein from Dahlia merckii expressed in Solanum melongena is released in root exudates and differentially affects pathogenic fungi and mycorrhizal symbiosis. New Phytol, 2004, 163: 393–403

[7]Segura A, Moreno M, Madueño F, Molina A, García-Olmedo F. Snakin-1, a peptide from potato that is active against plant pathogens. Mol Plant-Microbe Interact, 1999, 12: 16–23

[8]Jiang W(蒋雯), Liu X(刘欣), Zhang Z-Y(张增艳). Cloning and overexpression of defensin SN1 in Escherichia coli and its antifungal assay. J Plant Genet Resour (植物遗传资源学报), 2011, 12(2): 286–290 (in Chinese with English abstract)

[9]Alnasia N, Bazzini A, Hopp H E, Vazquez-Rovere C. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Plant Pathol, 2008, 9: 329–338

[10]Xu H-J(徐惠君), Pang J-L(庞俊兰), Ye X-G(叶兴国), Du L-P(杜丽璞), Li L-C(李连城), Xin Z-Y(辛志勇), Ma Y-Z(马有志), Chen J-P(陈剑平), Chen J(陈炯), Cheng S-H(程顺和), Wu H-Y(吴宏亚). Study on the gene transferring of Nib8 into wheat for its resistance to the Yellow mosaic virus by bombardment. Acta Agron Sin (作物学报), 2001, 27(6): 684–689 (in Chinese with English abstract)

[11]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325 (in Chinese with English abstract)

[12]Sharp P J, Kries M, Shewry P R, Gale M D. Location of amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75: 286–290

[13]Cai S-B(蔡士宾), Ren L-J(任丽娟), Yan W(颜伟), Wu J-Z(吴纪中), Chen H-G(陈怀谷), Wu X-Y(吴小有), Zhang X-Y(张仙义). Germplasm development and mapping of resistance to sharp eyespot (Rhizoctonia cerealis) in wheat. Sci Agric Sin (中国农业科学), 2006, 39(5): 928–934 (in Chinese with English abstract)

[14]Dong N, Liu X, Lu Y, Du L, Xu H, Liu H, Xin Z, Zhang Z. Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Func Integr Genomics, 2010, 10: 215–226

[15]Li Z, Zhou M, Zhang Z, Ren L, Du L, Zhang B, Xu H, Xin Z. Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Func Integr Genomics, 2011, 11: 63–70

[16]Jha S, Tank H G, Prasad B D, Chattoo B B. Expression of Dm-AMP1 in rice confers resistance to Magnaporthe oryzae and Rhizoctonia solani. Transgenic Res, 2009, 18: 59–69
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