Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1900-1907.doi: 10.3724/SP.J.1006.2012.01900

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Cytokinins on Formation of Seed Coat Colour in Oilseed Rape (Brassica napus)

WAN Hua-Fang,LU Dong,LIANG Ying*,SUN Fu-Jun,LI Jia-Na   

  1. College of Agronomy and Biotechnology, Southwest University / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
  • Received:2012-01-10 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 梁颖, E-mail: yliang@swu.edu.cn, Tel: 023-68250701

Abstract:

The seed color of yellow-seeded rape is closely related to seed maturity and plant hormone plays an important role in the regulation of seed maturity. This paper mainly focuses on the relationship between the cytokinin and the seed coat color of oilseed rape. Three pairs of rape seed with the same genetics background but different seed coat colors (yellow-seeded and black-seeded oil seed) were used to determine the dynamics of endogenous cytokinin (ZR), pigments and the activities of enzymes, as well as the aforementioned indices under the treatment with 6-BA. The results showed that the content of ZR in yellow-seeded rape was 4–5 times higher than that in black-seeded oneat 27 days after flowering,which was coupled with the less color of the rape seed; the content of ZR was negatively correlated with that of flavonoids, anthocyanin and melanin,and significantly positively correlated with polyphenols. Furthermore, it was significantly negatively correlated with tyrosinase activity, and not with the activity of phenylalnine ammonialyase or polyphenoloxidase; exogenous cytokinin (6-BA, 50 mg L–1) improved the color degree of the yellow seeds, and induced the decrease of the contents of melanin, anthocyanidin in seeds, it had no obvious effect on the black-seeded ones, but it increased the polyphenols content in the yellow seeds; the application of 6-BA decreased the activities of tyrosinase (TYR) and phenylalanine ammonia lyase (PAL). However, the activity of polyphenol oxidase (PPO) was not changed. Therefore, we concluded that cytokinin may significantly influence the seed coat color, and delay the pigment synthesis in the seed coat of yellow seeds through regulating the activities of enzymes involved in the pigment synthesis.

Key words: Rape, Seed coat color, Cytokinin, Pigment, 6-BA

[1]Xiao D-R(肖达人), Liu H-L(刘后利). The correlation analysis of seed coat color and seed oil content in Brassica napus. Acta Agron Sin (作物学报), 1982, 8(4): 245–254 (in Chinese with English abstract)



[2]Ye X-L(叶小利), Li J-N(李加纳), Tang Z-L(唐章林), Liang Y(梁颖), Chen L(谌利). Study on seed coat color and related characters of Brassica napus. Acta Agron Sin (作物学报), 2001, 27(5): 550–556 (in Chinese with English abstract)



[3]Ye X-L(叶小利), Li-J-N(李加纳), Tang-Z-L(唐章林), Chen L(谌利). The different color between black seed and yellow seed coat during seed development in Brassica napus: II. The changes and relating of Melanin, tyrosinase and tyrosinase. Chin J Oil Crop Sci (中国油料作物学报), 2001, 23(3): 38–41 (in Chinese with English abstract)



[4]Ye X-L(叶小利), Li J-N(李加纳), Tang Z-L(唐章林), Chen L(谌利). The different color between black seed and yellow seed coat during seed development in Brassica napus: I. the changes and relating of anthocyanin, phenylalanine and phenylalanine ammonialyase. Chin J Oil Crop Sci (中国油料作物学报), 2001, 23(2): 14–18 (in Chinese with English abstract)



[5]Guan C-Y(官春云). High-yield Rapeseed Cultivation Techniques (油菜优质高产栽培技术). Changsha: Hunan Science and Technology Press, 1997 (in Chinese)



[6]Pirie A, Mullins M G. Changes in anthocyanin and phenolics content of grapevine leaf and fruit tissue treated with sucrose, nitrate and abscisic acid. Plant Physiol, 1976, 58: 468–472



[7]Zhou X-Z(周旭章), Wei K-H(魏开华), Chen C-H(陈朝辉), Xie K(谢凯), Zhu J-J(朱建军). Research of extracting melanin from black sesame. J Chem Ind For Prod (林产化工通讯), 1997, (4): 17–19 (in Chinese)



[8]Zucker M. Light and its relation to chlorogenic acid synthesis in patato tuber tissue. Plant Physiol, 1965, 40: 779–784



[9]Tang S-G(唐尚格), Xia Y-X(夏玉先), Pei Y(裴炎). Indirect enzyme-linked immunosorbent assay endogenous plant hormones. J Southwest Agric Univ (西南农业大学学报), 1991, 13(2): 183–186 (in Chinese with English abstract)



[10]Li Y-W(李雨薇), Xiao L-T(肖浪涛). Detection technology of status and development for plant hormones. Life Sci Instr (生命科学仪器), 2007, 5(12): 10–13 (in Chinese with English abstract)



[11]Martin C A, Sharp W P. Alterations in leaf morphology of two landscape shrubs in response to disparate climate and paclobutrazol. Hort Sci, 1994, 29: 1321–1325



[12]Jones D H. Phenylalanine ammonia-lyase: regulation of its induction, and its role in plant development. Phytochemistry, 1984, 23: 1349–1359



[13]Yan G-H(闫国华), Gan L-J(甘立军), Sun R-H(孙瑞红). Gibberellin and cytokinin regulation of apple fruit growth mechanism of early. Acta Hort Sin (园艺学报), 2000, 27(1): 11–16 (in Chinese with English abstract)



[14]Pomerantz S H. Separation, purification, and properties of two tyrosinase from hamster melanoma. J Biol Chem, 1963, 238: 2351–2356



[15]Zhang Q(张琪), Cong P(丛鹏), Peng L(彭丽). Path Analysis in Excel and SPSS. Agric Network Inf (农业网络信息), 2007, (3): 109–111



[16]Gao X Q, Kong F J, Wang F, Matsuura H, Yoshihara H. Inhibitory role of gibberellins in theobroxide-induced flowering of Pharbitis nil. J Plant Physiol, 2006, 163: 398–404



[17]Liang Y-L(梁艳丽), Liang Y(梁颖), Li J-N(李加纳), Chen L(谌利). Comparison of the coat characteristics between yellow seed and black seed in Brassica napus. Chin J Oil Crop Sci (中国油料作物学报), 2001, 23(3): 38–41 (in Chinese with English abstract)



[18]Suzukieta1 R M. Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobiu. J Plant Physiol, 2004, 161: 929–935



[19]Ye X-L(叶小利), Li X-G(李学刚), Li J-N(李加纳). Formation mechanism of melanin in Brassica napus seed coat. Acta Agron Sin (作物学报), 2002, 28(5): 638–643 (in Chinese with English abstract)



[20]Bureau S, Renard C M G C, Reich M, Ginies C, Audergon J M. Change in anthocyanin concentrations in red apricot fruits during ripening. LWT-Food Sci Technol, 2009, 42: 372–377

[1] Ren Yi-Han, Zhao Man-Li, Dai Jing, Li Yin-Shui, Gu Chi-Ming, Yang Lu, Du Xue-Zhu, Hu Wen-Shi, Qin Lu. Effects of dynamic changes in leaf functional nitrogen on photosynthetic rate and photosynthetic nitrogen use efficiency in Brassica napus [J]. Acta Agronomica Sinica, 2026, 52(5): 1459-1471.
[2] Kuai Jie, Lou Hong-Xiang, Tan Xiao-Qiang, Gao Geng-Dong, Shao Dong-Li, Xiao Sheng-Nan, Zhao Jie, Xu Zheng-Hua, Wang Jing, Wang Bo, Zhou Guang-Sheng. Physiological basis and practical strategies for enhancing rapeseed yield under direct seeding [J]. Acta Agronomica Sinica, 2026, 52(4): 982-992.
[3] Zhang Quan-Jun, Wu Dong-Li, Liu Cong, Zhu Yong-Chao, Yang Da-Sheng, Kong Xiang-Sheng. Spatiotemporal evolution of rapeseed phenological periods in the Middle and Lower Reaches of the Yangtze River from 1981 to 2024 [J]. Acta Agronomica Sinica, 2026, 52(4): 1140-1152.
[4] Yang Rui, Chen Jing-Dong, Huang Ying, Zhang Xue-Kun, Zhou Deng-Wen, Liu Qing-Yun, Xu Jin-Song, Xie Ling-Li, Xu Ben-Bo. Study on breeding and cultivation strategies for winter rapeseed to cope with climate change in the lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2026, 52(4): 1153-1165.
[5] Tan Wen-Qing, Hui Rong-Kui, Zhang Fan-Li, Qin Lei, Mao Shu-Xiang, Deng Li-Chao, Guo Yi-Ming, Qu Liang, Yan Ming-Li. Rapid identification of waterlogging tolerance and selection of high waterlogging tolerance germplasm resources of rapeseed (Brassica napus L.) [J]. Acta Agronomica Sinica, 2026, 52(4): 1035-1045.
[6] Li Rui, Yu Yi-Wen, Wang Dun-Liang, Tian Ting, Sun Ling-Xiang, Tao Yue-Yue, Sun Hua. Comparative study on the characteristics of rapeseed yield under the rapeseed- oil dual-purpose mode in the middle and lower reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2026, 52(2): 620-630.
[7] Yang Rui, Chen Jing-Dong, Huang Ying, Zhang Xue-Kun, Zhou Deng-Wen, Liu Qing-Yun, Xu Jing-Song, Xie Ling-Li, Xu Ben-Bo. Region-specific yield optimization strategies for rapeseed (Brassica napus L.) in the middle Yangtze Basin across the 30°N latitude [J]. Acta Agronomica Sinica, 2026, 52(1): 99-117.
[8] Zhu Jia-Bao, Wang Xian-Ling, Fan You-Zhong, Wang Zong-Kai, Kuai Jie, Wang Bo, Wang Jing, Xu Zheng-Hua, Zhao Jie, Zhou Guang-Sheng. Effects of straw incorporation combined with nitrogen management on stem quality and lodging resistance of rapeseed following rice [J]. Acta Agronomica Sinica, 2026, 52(1): 233-248.
[9] FAN You-Zhong, WANG Xian-Ling, WANG Zong-Kai, WANG Chun-Yun, WANG Tian-Yao, XIE Jie, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, ZHAO Jie, ZHOU Guang-Sheng. Effects of straw incorporation combined with nitrogen management on photosynthetic efficiency and yield of rapeseed following rice [J]. Acta Agronomica Sinica, 2025, 51(8): 2139-2151.
[10] LI Bing-Lin, YE Xiao-Lei, XIAO Hong, XIAO Guo-Bin, LYU Wei-Sheng, LIU Jun-Quan, REN Tao, LU Zhi-Feng, LU Jian-Wei. Effects of magnesium fertilization rates on rapeseed yield, magnesium uptake, and yield loss caused by frost damage [J]. Acta Agronomica Sinica, 2025, 51(7): 1850-1860.
[11] LI Shi-Peng, CHEN Cai-Wu, ZHANG Jing, LYU Tian, FU Ting-Dong, YI Bin. Identification of fertility levels and quantification of the temperature-fertility relationship in rapeseed pol TCMS lines using an improved U-Net++ model [J]. Acta Agronomica Sinica, 2025, 51(6): 1423-1434.
[12] CUI Xin, GU He-He, SONG Yi, ZHANG Zhe, LIU Shi-Shi, LU Zhi-Feng, REN Tao, LU Jian-Wei. Effects of potassium fertilizer application rates on rapeseed yield and potassium absorption and yield reduction caused by frost damage [J]. Acta Agronomica Sinica, 2025, 51(6): 1629-1642.
[13] WANG Qing-Hua, ZHU Ge-Ge, FANG Wen, LIU Shi-Shi, LU Jian-Wei. Diagnosis of nitrogen and phosphorus nutrient content in rapeseed leaves based on hyperspectral remote sensing [J]. Acta Agronomica Sinica, 2025, 51(5): 1326-1337.
[14] WANG Meng-Ning, XIE Ke-Ran, GAO Ti, WANG Fei, REN Xiao-Jian, XIONG Dong-Liang, HUANG Jian-Liang, PENG Shao-Bing, CUI Ke-Hui. Effect of high temperature during the panicle initiation and heading stages on grain shape and filling and its relationship with grain weight in rice [J]. Acta Agronomica Sinica, 2025, 51(5): 1347-1362.
[15] WANG Jia-Jie, WANG Zheng-Nan, BATOOL Maria, WANG Wang-Nian, WEN Jing, REN Chang-Zhong, HE Feng, WU You-You, XU Zheng-Hua, WANG Jing, KUAI Jie, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong. Comparison of physiological characteristics of salt and alkali tolerance between rapeseed and wheat [J]. Acta Agronomica Sinica, 2025, 51(5): 1215-1229.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!