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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1900-1907.doi: 10.3724/SP.J.1006.2012.01900


Effect of Cytokinins on Formation of Seed Coat Colour in Oilseed Rape (Brassica napus)

WAN Hua-Fang,LU Dong,LIANG Ying*,SUN Fu-Jun,LI Jia-Na   

  1. College of Agronomy and Biotechnology, Southwest University / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
  • Received:2012-01-10 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 梁颖, E-mail: yliang@swu.edu.cn, Tel: 023-68250701


The seed color of yellow-seeded rape is closely related to seed maturity and plant hormone plays an important role in the regulation of seed maturity. This paper mainly focuses on the relationship between the cytokinin and the seed coat color of oilseed rape. Three pairs of rape seed with the same genetics background but different seed coat colors (yellow-seeded and black-seeded oil seed) were used to determine the dynamics of endogenous cytokinin (ZR), pigments and the activities of enzymes, as well as the aforementioned indices under the treatment with 6-BA. The results showed that the content of ZR in yellow-seeded rape was 4–5 times higher than that in black-seeded oneat 27 days after flowering,which was coupled with the less color of the rape seed; the content of ZR was negatively correlated with that of flavonoids, anthocyanin and melanin,and significantly positively correlated with polyphenols. Furthermore, it was significantly negatively correlated with tyrosinase activity, and not with the activity of phenylalnine ammonialyase or polyphenoloxidase; exogenous cytokinin (6-BA, 50 mg L–1) improved the color degree of the yellow seeds, and induced the decrease of the contents of melanin, anthocyanidin in seeds, it had no obvious effect on the black-seeded ones, but it increased the polyphenols content in the yellow seeds; the application of 6-BA decreased the activities of tyrosinase (TYR) and phenylalanine ammonia lyase (PAL). However, the activity of polyphenol oxidase (PPO) was not changed. Therefore, we concluded that cytokinin may significantly influence the seed coat color, and delay the pigment synthesis in the seed coat of yellow seeds through regulating the activities of enzymes involved in the pigment synthesis.

Key words: Rape, Seed coat color, Cytokinin, Pigment, 6-BA

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