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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 2034-2041.doi: 10.3724/SP.J.1006.2012.02034

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Development of a Functional Marker of TaABP1-D Gene Associated with Plant Height in Bread Wheat

QIAO Lin-Yi1,**,ZHANG Lei1,**,ZHANG Wen-Ping1,ZHAO Guang-Yao2,WANG Xi1,*,JIA Ji-Zeng2,*   

  1. 1 College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Gene Resources & Germplasm Enhancement, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2012-03-22 Revised:2012-06-15 Online:2012-11-12 Published:2012-09-10
  • Contact: 王玺, E-mail: wxi2000@126.com; 贾继增, E-mail: jzjia@mail.caas.net.cn

Abstract:

AUXIN BINDING PROTEIN1 (ABP1) is one of the ?rst characterized proteins that binds auxin. It ful?lls the criteria for an auxin receptor and plays an important role in auxin responses on the plasma membrane. In this study, the genomic sequence of a novel gene, designated TaABP1, isolated from the hexaploid wheat (Triticum aestivum L.) Chinese Spring genome databases. The result of chromosomal location showed that TaABP1 was on homoelogous group 5 of chromosomes in wheat. Then TaABP1-D was cloned in Chinese Spring. Sequence analysis showed that the complete open reading frame (ORF) of TaABP1-D was 1887 bp in length, encoding a putative protein composed of 205 amino acids with theendoplasmic reticulum retention sequence Lys-Asp-Glu-Leu (KDEL) and box regions. The expression of TaABP1-D was detected in root, basal stem, upper stem, and leaf tip of wheat at jointing stage with the expression levels as leaf tip > upper stems > root > base stem. Sequence alignment proved that TaABP1-D shared high homology with rice OsABP1 (82% protein sequence identity) and other plant ABPs reported. Using a population of recombinant inbred lines from the cross between W7984 and Opata85, an SSR maker was developed based on (GT)6/5 divergence in the upstream sequence of TaABP1-D. This marker was proved to be highly correlated with plant height, and the phenotypic variation explained was 9.7%. The W7984 type allelic variation for tall plant was specific in wild varieties, and eliminated in cultivated varieties. This indicates that the favorable variation of TaABP1-D has been fixed in cultivated varieties of wheat, and a bottleneck effect might occur during the domestication of wheat.

Key words: Wheat, TaABP1-D, Homologous cloning, Expression analysis, Functional marker, Association analysis

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