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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1983-1991.doi: 10.3724/SP.J.1006.2013.01983

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genotypic Diversity at the Lr10 Locus for Leaf Rust Resistance in Various Hexaploid Wheat Varieties (lines)

HAN Shu-Xiao1,LIU Quan-Lan2,DONG Jie1,CHEN Jian-Sheng1,TIAN Ji-Chun1,*   

  1. 1 State Key Laboratory of Crop Biology / Shandong Provincial Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China;
    2 Department of Bioengineering and Biotechnology, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
  • Received:2013-01-24 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 田纪春, E-mail: jctian@sdau.edu.cn, Te1: 0538-8242040 E-mail:hanshuxiao51444@163.com

Abstract:

Genotypes H1 and H2 are two ancient haplotypes on Lr10 locus, which is a resistance gene to wheat leaf rust. They are identified based on the presence of both Lr10 and RGA2 in full length (H1), and the absence of Lr10 and chromatin rearrangement of RGA2 (H2) due to chromosome 1AS reorganization during species evolution. Both haplotypes contain several subhaplotypes. To understand genetic diversity on Lr10 locus, we tested the frequencies of H1 and H2 haplotypes and their subhaplotypes in 189 wheat cultivars and 58 breeding lines originating from various provinces of China. The H2 haplotype was dominant in the developed cultivars and breeding lines with the frequencies of 95.2% (180/189) and 96.6% (56/58), respectively. Five novel subhaplotypes were detected in the developed cultivars, namely H1-2, H2-4, H2-5, H2-6, and H2-7. In cultivars, subhaplotype H2-1 was the most frequent (69.3%), whereas subhaplotypes H2-3 and H2-6 had the lowest frequencies (0.5%). Among the five subhaplotypes detected in breeding lines, subhaplotype H2-1 had the highest frequencies (27.6%), whereas subhaplotypes H1-2 was the least frequent (3.5%). Interestingly, the subhaplotypes except for H2-1 and H2-2 were highly breeding location-dependent (P < 0.05). These results support the hypothesis that both haplotypes have been maintained through a balanced polymorphism mechanism during wheat domestication. Furthermore, the five novel subhaplotypes suggest that genetic diversities resulting from recombination and other types of chromatin reorganizations (e.g., origin of the H2 haplotype and polymorphism for the Lr10 locus) have been going on since the formation of the hexaploid wheat. Significantly, identification of the H1-2 subhaplotype is a clear evidence of occurrence of recombination between the two ancient haplotypes, which disagrees with the previous notion. Importantly, because the frequencies of H1 is lower than 5% in developed cultivars and breeding lines, actions for protecting germplasm with the H1 haplotype are highly recommended.

Key words: Wheat, Lr10, Ancient haplotype, Subhaplotype, Diversity

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