Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 2055-2064.doi: 10.3724/SP.J.1006.2013.02055

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Comparison of Microecological Characterization in Rhizosphere Soil between Healthy and Un-healthy Plants in Continuous Cropping Potato Fields

CHEN Jie1,GUO Tian-Wen2,TAN Xue-Lian2,ZHU Wei-Bing3,WEI Xiao-Li1,WANG Dong-Sheng1,XUE Quan-Hong1,*   

  1. 1 College of Resources and the Environment, Northwest A&F University, Yangling 712100, China; 2 Dryland Agriculture Institute Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 Yangling Jinshu Sweet Potato Seed Technology Limited, Yangling 712100, China
  • Received:2013-01-14 Revised:2013-06-09 Online:2013-11-12 Published:2013-08-12
  • Contact: 薛泉宏, E-mail: xuequanhong@163.com, Tel: 13474173220

RichHTML

PDF

1348

Cited

3

Abstract:

To explore the microecological mechanism of healthy plant growth in continuous potato fields in Gansu Province, China, we measured contents of available soil nitrogen (NH4+-N), phosphorus (P), and potassium (K) using conventional soil nutrient analytic methods, abundance of soil actinomycetes using serial dilution and plating techniques, and screened antagonistic actinomycetes from obtained actinomycete isolates using the agar block method. The selected dominant actinomycetes were identified by 16S rRNA sequence analysis, and the inhibitory effect of one dominant actinomycete, Streptomyces gancidicus, was tested using culture filtrate. Results showed that in the four-year continuous potato field, available soil P and K contents in the rhizosphere of diseased plants were respectively 29.9% and 12.5% lower than those of healthy plants, whereas soilNH4+-N content in the rhizosphere of diseased plant was 24.1% higher than that of healthy plant. Compared with those fromthe rhizosphere of healthy plants, the number of soil actinomycetes, Micromonospora, and unidentified actinomycetes from the rhizosphere of diseased plants cultured with the Gause1 medium decreased by 51.1%, 83.0%, and 53.9%, respectively, whereas the number of soil actinomycetes and Streptomyces from the rhizosphere of diseased plants cultured with the Humic Acid medium decreased by 46.0% and 46.7%, respectively. The soil actinomycetes antagonistic potentiality (SAAP) of actinomycetes with antagonistic effects on thefour pathogenic fungi of potato were lower in the rhizosphere of diseased plants than in the rhizosphere of healthy plants. S. gancidicus and S. galilaeus were found dominant in the rhizosphere of healthy and diseased plants, respectively. In conclusion, the microecology of rhizosphere soil that supports healthy plant growth in the continuous potato field is mainly characterized by: available nutrient combination of abundant P and K with relatively poor N contents; and a larger number of soil actinomycetes than that of diseased plants, with the numerically dominant actinomycetes showing beneficial antagonistic effect on several common soil-borne pathogenic fungi.

Key words: Potato, Continuous cropping, Soil nutrient, Actinomycetes, Microecological mechanism

[1]Zhang J-D(张久东), Bao X-G(包兴国), Yang W-Y(杨文玉), Hu Z-Q(胡志桥), Wang T(王婷), Li Q-F(李全福). High-efficiency cultivation technique of potato by intercropping green manure plants. Gansu Agric Sci Technol (甘肃农业科技), 2011, 12: 52–53 (in Chinese)



[2]Yu B(余斌), Shen B-Y(沈宝云), Wang W(王文), Qiu H-Z(邱慧珍), Liu X(刘鑫), Li Z-Z(李朝周), Zhang J-L(张俊莲), Wang D(王蒂). Effects of different potato varieties on preventing continuous cropping obstacle in the arid regions. J Gansu Agric Univ (甘肃农业大学学报), 2012, 47(4): 49–53 (in Chinese with English abstract) 



[3]Robert P L, Timothy S G. Control of soilborne potato diseases using Brassica green manures. Crop Protection, 2007, 26: 1067–1077



[4]Bai S(白霜), Xue Q-H(薛泉宏), Zhao Y-C(赵邑尘), Cao S-M(曹书苗), Tong Y-A(同延安), Wang X-H(王晓辉), Xu W-L(徐万里). Study on actinomycetic ecology between healthy and diseased plants & rhizosphere with different salt contents in Xinjiang. J Northwest A&F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2009, 37(7): 183–190 (in Chinese with English abstract)



[5]Duan C-M(段春梅), Xue Q-H(薛泉宏), Hu S-B(呼世斌), Zhao J(赵娟), Wei Y(魏样), Wang L-N(王玲娜), Shen G-H(申光辉), Chen Q(陈秦). Microbial ecology of fusarium wilt infected and healthy cucumber plant in root zone of continuous cropping soil. J Northwest A& F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2010, 38(4): 143–150 (in Chinese with English abstract)



[6]Shen G-H(申光辉), Xue Q-H(薛泉宏), Zhang J(张晶), Duan J-L(段佳丽), Wang D-S(王东胜), Yang X-H(杨兴华). Screening, identification and biocontrol potential of antagonistic fungi against strawberry root rot and plant growth promotion. Sci Agric Sin (中国农业科学), 2012, 45(22): 4612–4626 (in Chinese with English abstract)



[7]Cheng L-J(程丽娟), Xue Q-H(薛泉宏). Experimental Techniques of Microbiology (微生物实验技术). Xi’an: World Publishing Corporation, 2000. pp 80–84, 383–384 (in Chinese)



[8]Xu L-H(徐丽华), Jiang C-L(姜成林). Studies of Microbial Resources (微生物资源学). Beijing: Science Press, 1997 (in Chinese)



[9]Sun J-Z(孙敬祖), Xue Q-H(薛泉宏), Tang M(唐明), Cao S-M(曹书苗), Xing S-L(邢胜利). Study on the effect of actinomycetes on microflora of replanted strawberry’s root domain and the bio-control effectiveness. J Northwest A& F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2009, 37(12): 153–158(in Chinese with English abstract)



[10]Bao S-D(鲍士旦). Soil and agricultural chemistry analysis(土壤农化分析). Beijing, China Agriculture Press, 2000. pp 30–34, 53–56, 81–83, 106–108, 165 (in Chinese)



[11]Zhu W-J(朱文杰), Xue Q-H(薛泉宏), Cao Y-R(曹艳茹), Xue L(薛磊), Shen G-H(申光辉), Lai H-X(来航线). Distribution and characteristics of soil antagonistic actinomycetes on northern slope of Taibai Mountain, Qinling. Chin J Appl Ecol (应用生态学报), 2011, 22(11): 3003–3010 (in Chinese with English abstract)



[12]Xu L-H(徐丽华), Li W-J(李文均), Liu Z-H(刘志恒). Actinomycetes Systematics: Principle, Method and Practice (放线菌系统学:原理方法及实践). Beijing, Science Press, 2007. pp 40–45 (in Chinese)



[13]Zhao J(赵娟), Xue Q-H(薛泉宏), Wang L-N(王玲娜), Duan C-M(段春梅), Xue L(薛磊), Mao N(毛宁). Antagonistic effect of multifunctional actinomycete strain Act12 on soil-borne pathogenic fungi and its identification. Chin J Eco-Agric (中国生态农业学报), 2011, 19(2): 394–398 (in Chinese with English abstract)



[14]Hu Y(胡宇), Guo T-W(郭天文), Zhang X-C(张绪成). Effect of potato continuous cropping on soil nutrients in dry land. J Anhui Agric Sci (安徽农业科学), 2009, 37(12): 5436–5439, 5610 (in Chinese with English abstract)



[15]Sun Q(孙权), Chen R(陈茹), Song N-P(宋乃平), Wang Q-F(王青凤), Wang R(王锐). Change trends of soil nutrients, enzyme activities, and microbial composition in continuous potato cropping system in semi-arid and cool hilly area of Ningxia. J Soil Water Conserv (水土保持学报), 2010, 24(6): 208–212 (in Chinese with English abstract)



[16]Zhang H-Y(张鸿雁), Xue Q-H(薛泉宏), Tang M(唐明), Wang L-N(王玲娜), Duan C-M(段春梅). Study on actinomycetic ecology in soil with the different cultivating years of ginseng. J Northwest A&F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2010, 38(8): 151–159 (in Chinese with English abstract)



[17]Zhang M(张萌), Zhao W-Q(赵伟全), Yu X-M(于秀梅), Yang W-X(杨文香), Zhang T(张汀), Liu D-Q(刘大群). Genetic diversity of potato scab pathogens in China based on 16S rDNA sequences. Sci Agric Sin (中国农业科学), 2009, 42(2): 499–504 (in Chinese with English abstract)
[1] WANG Hai-Bo, YING Jing-Wen, HE Li, YE Wen-Xuan, TU Wei, CAI Xing-Kui, SONG Bo-Tao, LIU Jun. Identification of chromosome loss and rearrangement in potato and eggplant somatic hybrids by rDNA and telomere repeats [J]. Acta Agronomica Sinica, 2022, 48(5): 1273-1278.
[2] SHI Yan-Yan, MA Zhi-Hua, WU Chun-Hua, ZHOU Yong-Jin, LI Rong. Effects of ridge tillage with film mulching in furrow on photosynthetic characteristics of potato and yield formation in dryland farming [J]. Acta Agronomica Sinica, 2022, 48(5): 1288-1297.
[3] FENG Ya, ZHU Xi, LUO Hong-Yu, LI Shi-Gui, ZHANG Ning, SI Huai-Jun. Functional analysis of StMAPK4 in response to low temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(4): 896-907.
[4] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[5] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[6] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[7] TAN Xue-Lian, GUO Tian-Wen, HU Xin-Yuan, ZHANG Ping-Liang, ZENG Jun, LIU Xiao-Wei. Characteristics of microbial community in the rhizosphere soil of continuous potato cropping in arid regions of the Loess Plateau [J]. Acta Agronomica Sinica, 2022, 48(3): 682-694.
[8] ZHANG Hai-Yan, XIE Bei-Tao, JIANG Chang-Song, FENG Xiang-Yang, ZHANG Qiao, DONG Shun-Xu, WANG Bao-Qing, ZHANG Li-Ming, QIN Zhen, DUAN Wen-Xue. Screening of leaf physiological characteristics and drought-tolerant indexes of sweetpotato cultivars with drought resistance [J]. Acta Agronomica Sinica, 2022, 48(2): 518-528.
[9] JIAN Hong-Ju, SHANG Li-Na, JIN Zhong-Hui, DING Yi, LI Yan, WANG Ji-Chun, HU Bai-Geng, Vadim Khassanov, LYU Dian-Qiu. Genome-wide identification and characterization of PIF genes and their response to high temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 86-98.
[10] XU De-Rong, SUN Chao, BI Zhen-Zhen, QIN Tian-Yuan, WANG Yi-Hao, LI Cheng-Ju, FAN You-Fang, LIU Yin-Du, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of StDRO1 gene polymorphism and association analysis with root traits in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 76-85.
[11] ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[12] SONG Tian-Xiao, LIU Yi, RAO Li-Ping, Soviguidi Deka Reine Judesse, ZHU Guo-Peng, YANG Xin-Sun. Identification and expression analysis of cell wall invertase IbCWIN gene family members in sweet potato [J]. Acta Agronomica Sinica, 2021, 47(7): 1297-1308.
[13] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[14] LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping. Key genes mining of DNA methylation involved in regulating drought stress response in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 599-612.
[15] QIN Tian-Yuan, LIU Yu-Hui, SUN Chao, BI Zhen-Zhen, LI An-Yi, XU De-Rong, WANG Yi-Hao, ZHANG Jun-Lian, BAI Jiang-Ping. Identification of StIgt gene family and expression profile analysis of response to drought stress in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 780-786.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd