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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 86-92.doi: 10.3724/SP.J.1006.2014.00086

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Production of Very Long Chain Polyunsaturated Fatty Acids in Cotton

LIU Jiang1,**,MA Yan-Bin2,**,SUN Quan-Xi1,3,WU Xia2,LI Xue-Ying1,SUN Mei-Hong1,LI Yan-E2,LI Xin-Zheng1,*,QI Bao-Xiu1,*   

  1. 1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018; 2 Coton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China; 3 Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2013-05-18 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-23
  • Contact: 亓宝秀, E-mail: qbx126@sdau.edu.cn, Tel: 0538-8246205; 李新征, E-mail: lxz@sdau.edu.cn, Tel: 0538-8246205 E-mail:liujiang2582006@126.com

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Abstract:

We have isolated four genes encoding a Δ9 elongase, a Δ8 desaturase, a Δ5 desaturase, and a Δ15 desaturase from Isochrysis galbana, Euglena gracilis, Mortierella alpina and Arabidopsis thaliana respectively. Using a multigene transfer technology that we developed, these genes were stacked together in the plant expression vector pCambia2300. Each gene contained its own CaMV35S promoter and Tnos terminator.This plant expression vector was then transferred into cotton by Agrobacterium-mediated transformation method. Transgenic cotton seedlings were first identified by screening them based on kanamycin-containing media and followed by PCR with gene-specific primers of the four transgenes. Finally, these transgenic plants were subjected to gas liquid chromatography analysis for their fatty acid composition and the results showed that the contents of arachidonic acid (ARA, 20:4Δ5,8,11,14) and eicosapentaenoic acid (EPA, 20:5Δ5,8,11,14,17) were 1.0% and 5.0% respectively in the leaves of the transgenic plants, indicating that the four genes were expressed incotton. Therefore, our data clearly demonstrated the feasibility for the heterologous production of EPA in cotton and this will lay a foundation for the production of VLCPUFAs, including EPA and DHA in cotton seed through transgenic technology in the future.

Key words: Desaturase, Elongase, Transgenes, Eicosapentaenoic acid, Cotton

[1]Abbadi A, Domergue F, Bauer J, Napier J A, Welti R, Zahringer U, Cirpus P, Heinz E. Biosynthesis of very long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell, 2004, 16: 2734–2748



[2]Funk C D. Prostaglandins and leukotrienes: advances in eicosanoid biology. Science, 2001, 294: 1871–1875



[3]Simopoulos A P. Omega-3 fatty acids in inflammation and autoimmune diseases. J Am Coll Nutr, 2002, 21: 495–505



[4]Uauy R, Hoffman D R, Peirano P, Birch D G, Birch E E. Essential fatty acids in visual and brain development. Lipids, 2001, 36: 885–895



[5]Qi B X, Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier J A, Lazarus C M. Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat Biotechnol, 2004, 22: 739–745



[6]Lu C F, Napier J A, Clemente T E, Cahoon E B. New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications. Curr Opin Plant Biol, 2011, 22: 252–259 



[7]Venegas-Calerón M, Sayanova O, Napier J A. An alternative to fish oils: metabolic engineering of oil-seed crops to produce omega-3 long chain polyunsaturated fatty acids. Prog Lipid Res, 2010, 49: 108–119



[8]Wu G, Truksa M, Datla N, Vrinten P, Bauer J, Zank T, Cirpus P, Heinz E, Qiu X. Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat biotechnol, 2005, 23: 1013–1017



[9]Hoffmann M, Wagner M, Abbadi A, Fulda M, Feussner I. Metabolic engineering of ω3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J Biol Chem, 2008, 283: 22352–22362



[10]杨艳, 王贤磊, 李冠. 六种新疆陆地棉棉籽脂肪酸成分分析. 生物技术, 2009, 19(4): 54–56



Yang Y, Wang X L, Li G. Analysison fatty acid composition of six species of upland cotton. Biotechnology, 2009, 19(4): 54–56 (in Chinese with English abstract)



[11]Qi B X, Beaudoin F, Fraser T, Stobart A K, Napier J A, Lazarus C M. Identification of a cDNA encoding a novel C18-Delta(9) polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS Lett, 2002, 510: 159–165



[12]Wallis J G, Browse J. The Delta8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. Arch Biochem Biophys, 1999, 365: 307–316



[13]Michaelson L V, Lazarus C M, Griffiths G, Napier J A, Stobart A K. Isolation of a Delta5-fatty acid desaturase gene from Mortierella alpina. J Biol Chem, 1998, 273: 19055–19059



[14]Yadav N S, Wierzbicki A, Aegerter M, Caster C S, Perez-Grau L, Kinney A J, Hitz W D, Booth J R, Schweiger B, Stecca K L. Cloning of higher plant omega-3 fatty acid desaturases. Plant Physiol, 1993, 103: 467–476



[15]Sun Q X, Liu J, Li Y X, Zhang Q, Shan S H, Li X Z, Qi B X. Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant. Plant Mol Biol, 2013, DOI:10.1007/s11103-013-0096-2



[16]秦永华, 乔志新, 刘进元. 转基因技术在棉花育种上的应用. 棉花学报, 2007, 19: 482–488



Qin Y H, Qiao Z X, Liu J Y. Application of genetic transformation in cotton breeding. Cotton sci, 2007, 19: 482–488 (in Chinese with English abstract)



[17]Halpin C. Gene stacking in transgenic plants--the challenge for 21st century plant biotechnology. Plant Biotechnol, 2005, 3: 141–155



[18]Sun Q, Liu J, Zhang Q, Qing X, Dobson G, Li X Z, Qi B X. Characterization of three novel desaturases involved in the delta-6 desaturation pathways for polyunsaturated fatty acid biosynthesis from Phytophthora infestans. Appl Microbiol Biotechnol, 2012, DOI: 10.1007/s00253-012-4613-z



[19]Cheng B F, Wu G H, Vrinten P, Falk K, Bauer J, Qiu X. Towards the production of high levels of eicosapentaenoic acid in transgenic in plants: the effects of different host species, genes and promoters. Transgenic Res, 2010, 19: 221–229



[20]Lu C F, Napier J A, Clemente T E, Cahoon E B. New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications. Curr Opin Plant Biol, 2011 22: 252–259 



[21]Domergue F, Abbadi A, Heinz E. Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trends Plant Sci, 2005, 10: 112–116
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