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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1557-1564.doi: 10.3724/SP.J.1006.2014.01557

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Resistance of Slow Mildewing Genes to Stripe Rust and Leaf Rust in Common Wheat

LIU Jin-Dong1,CHEN Xin-Min1,HE Zhong-Hu1,2,WU Ling3,BAI Bin4,LI Zai-Feng5,XIA Xian-Chun1,*   

  1. 1 Institute of Crop Science/ National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China; 2CIMMYT-China Office, c/o CAAS, Beijing 100081, China; 3Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 4Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 5Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
  • Received:2014-02-05 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-09
  • Contact: 夏先春, E-mail: xiaxianchun@caas.cn, Tel: 010-82108610

Abstract:

Pyramiding quantitative trait loci (QTLs) is an effective method to improve resistance to powdery mildew, stripe rust, and leaf rust in common wheat. We have developed 21 lines (F6) carrying 2-5 slow mildewing QTLs by crossing slow powdery mildew cultivars Bainong 64 and Lumai 21 possessing four and three slow mildewing QTLs, respectively. These F6 lines were evaluated in the field in Pianxian, Sichuan and Tianshui, Gansu for stripe rust resistance and in Baoding, Hebei and Zhoukou, Henan for leaf rust resistance during the 2012-2013 cropping season. According to the maximum disease severities (MDS) and the area under the disease progress curve (AUDPC), QTLs QPm.caas-4DL, QPm.caas-6BS and QPm.caas-2BL were highly resistant to stripe rust (P < 0.01), which explained 16.9%, 14.1%, and 17.3% of phenotypic variance, respectively. Locus QPm.caas-4DL also showed high resistance to leaf rust (P < 0.01) with phenotypic contribution of 35.3%. Lines that pyramided five (QPm.caas-1A/QPm.caas-4DL/ QPm.caas-2DL/QPm.caas-2BS/QPm.caas-2BL) and four (QPm.caas-1A/QPm.caas-4DL/QPm.caas-2BS/QPm.caas-2BL) QTLs exhibited higher resistance to both stripe and leaf rust compared with their parents. This result indicates that the combination of QPm.caas-4DL (from Bainong 64), QPm.caas-2BS and QPm.caas-2BL (Lumai 21) has a marked effect on improving adult resistance to powdery mildew, stripe rust and leaf rust, and the more QTLs are pyramided, the stronger slow disease resistance can be achieved. In breeding practice, the combination of 4-5 slow mildewing or rusting QTLs can result in durable resistance to multiple diseases.

Key words: Triticum aestivum L., Slow mildewing and slow rusting resistance, Durable resistance, Gene pyramiding, QTL

[1]Wellings C R, Mcintosh R A, Hussain M. A new source of resistance to Puccinia striiformis f. sp. tritici in spring wheats (Triticum aestivum). Plant Breed, 1988, 100: 288–296



[2]Komer J A. Genetics of resistance to wheat leaf rust. Annu Rev Phytopathol, 1996, 34: 435–455



[3]Conner R L, Kuzyk A D, Su H. Impact of powdery mildew on the yield of soft white spring wheat cultivars. Can J Plant Sci, 2003, 83: 725–728



[4]Singh R P, Huerta-Espino J, William H M. Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turk J Agric For, 2005, 29: 121–127



[5]Roberts J, Caldwell R M. General resistance (slow mildewing) to Erysiphe graminis f. sp. tritici in Knox wheat. Mol Gen Genet, 1970, 60: 1310



[6]Gustafson G D, Shaner G. Influence of plant age on the expression of slow-mildewing resistance in wheat (Triticum aestivum). Phytopathology, 1982, 72: 746–749



[7]Singh R P, Huerta-Espino J, Bhavani S, Herrera-Foessel S A, Singh D, Singh P K, Velu G, Mason R E, Jin Y, Njau P, Crossa J. Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica, 2011, 179: 175–186



[8]Lu Y M, Lan C X, Liang S S, Zhou X C, Liu D, Zhou G, Lu Q L, Jing J X, Wang M N, Xia X C, He Z H. QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theor Appl Genet, 2009, 119: 1349–1359



[9]Lan C X, Liang S S, Zhou X C, Zhou G, Lu Q L, Xia X C, He Z H. Identification of genomic regions controlling adult-plant stripe rust resistance in Chinese landrace Pingyuan 50 through bulked segregant analysis. Phytopathology, 2010, 100: 313–318.



[10]Krattinger S G, Lagudah E S, Spielmeyer W, Singh R P, Huerta-Espino J, Mcfadden H, Bossolini E, Selter L L, Keller B. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 2009, 323: 1360–1363



[11]Bhavani S, Singh R P, Argillier O, Huerta-Espino J, Singh S, Njau P, Brun S, Lacam S, Desmouceaux N. Mapping Durable Adult Plant Stripe Rust Resistance to the Race Ug99 Group in Six CIMMYT Wheats. BGRI 2011 Technical Workshop, St. Paul, Minnesota, 2011. pp 44–53



[12]Singh R P, Huerta-Espino J, Rajaram S. Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung, 2000, 35: 133–139



[13]Castro A J, Chen X M, Hayes P M, Johnston M. Pyramiding quantitative trait locus (QTL) alleles determining resistance to barley stripe rust: effects on resistance at the seedling stage. Crop Sci, 2003, 43: 651–659



[14]Marasas C N, Smale M, Singh R P. The impact of agricultural maintenance research: the case of leaf rust resistance breeding in CIMMYT-related spring bread wheat. In: CD-ROM Proceeding Internal Congress on Impacts of Agricultural Research and Development. San Jose, Costa Rica, 2002



[15]Singh R P, William H M, Huerta-Espino J, Rosewarne G. Wheat rust in Asia: meeting the challenges with old and new technologies. In: New Directions for a Diverse Planet. Proceedings of the 4th International Crop Science Congress. Brisbane, Australia, 2004, p 26



[16]Dekkers J C M, Hospital F. The use of molecular genetics in the improvement of agricultural populations. Nat Rev Genet, 2003, 3: 22–32



[17]Miedaner T, Wilde F, Steiner B, Buerstmayr H, Korzun V, Ebmeyer E. Stacking quantitative trait loci (QTL) for Fusarium head blight resistance from non-adapted sources in an European elite spring wheat background and assessing their effects on deoxynivalenol (DON) content and disease severity. Theor Appl Genet, 2006, 112: 562–569



[18]Lu Q X, Szabo-Hever A, Åsmund B, Lillemo M, Semagn K, Mesterhazy A, JiF, Shi J R, Skinnes H. Two major resistance quantitative trait loci are required to counteract the increased susceptibility to Fusarium head blight of the Rht-D1b dwarfing gene in wheat. Crop Sci, 2011, 51: 2430–2438



[19]Wang Z L, Li L H, He Z H, Duan X Y, Zhou Y L, Chen X M, Lillemo M, Singh R P, Wang H, Xia X C. Seeding and adult-plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis, 2005, 89: 457–463



[20]任妍. 普通小麦抗条锈病基因分子定位. 中国农业科学院博士学位论文, 北京, 2012. pp 56–63



Ren Y. Molecular Mapping of Stripe Rust Resistance Genes in Common Wheat. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2012. pp 56-63 (in Chinese with English abstract)



[21]Lan C X, Liang S S, Wang Z L, Yan J, Zhang Y, Xia X C, He Z H. Quantitative trait loci mapping for adult-plant resistance to powdery mildew in Chinese wheat cultivar Bainong 64. Phytopathology, 2009, 99: 1121–1126



[22]Lan C X, Ni X W, Yan J, Zhang Y, Xia X C, Chen X M, He Z H. Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Mol Breed, 2010, 25: 615–622



[23]Bai B, He Z H, Asad M A, Lan C X, Zhang Y, Xia X C, Yan J, Chen X M, Wang C S. Pyramiding adult-plant powdery mildew resistance QTLs in bread wheat. Crop Pasture Sci, 2011, 63: 606–611



[24]Lin F, Chen X M. Quantitative trait loci for non-race-speci?c, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express. Theor Appl Genet, 2009, 118: 631–642



[25]Ren Y, Li Z F, He Z H, Wu L, Bai B, Lan C X, Wang C F, Zhou G, Zhu H Z, Xia X C. QTL mapping of adult-plant resistance to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64. Theor Appl Genet, 2012, 125: 1253–1262



[26]Lillemo M, Asalf B, Singh R P, Huerta-Espino J, Chen X M, He Z H, Bjørnstad Å. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet, 2008, 116: 1155–1166



[26]Singh R P. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology, 1992, 82: 835–838



[28]Dyck P L, Kerber E R, Aung T. An interchromosomal reciprocal translocation in wheat involving leaf rust resistance gene Lr34. Genome, 1994, 37: 556–559



[29]Herrera-Foessel S A, Lagudah E S, Huerta-Espino J, Hayden M, Bariana H S, Singh R P. New slow-rusting leaf rust and stripe rust resistance gene Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet, 2011, 122: 239–249



[30]何中虎, 兰彩霞, 陈新民, 邹裕春, 庄巧生, 夏先春. 小麦条锈病和白粉病成株抗性研究进展和展望. 中国农业科学, 2011, 44: 2193–2215



He Z H, Lan C X, Chen X M, Zou Y C, Zhuang Q S, Xia X C. Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Sci Agric Sin, 2011, 44: 2193–2215 (in Chinese with English abstract)



[31]Marasas C N, Smale M, Singh R P. The economic impact of productivity maintenance research: breeding for leaf rust resistance in modern wheat. Agric Eco, 2003, 29: 253–263



[32]Chen X M, Line R F. Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis. Phytopathology, 1995, 85: 567–572



[33]Bariana H S, Kailasapillai S, Brown G N, Sharp P J. Marker assisted identification of Sr2 in the National Cereal Rust Control Program in Australia, In: Slinkard A E ed. Proc 9th Intl Wheat Genet Symp. Vol. 5. University of Saskatchewan, Saskatoon, SK, Canada: Univ. Extension Press, 1998. pp 83−91



[34]Keller M, Keller B, Schachermayr G, Winzeler M, Schmid J E, Stamp P, Messmer M M. Quantitative trait loci for resistance against powdery mildew in a segregating wheat × spelt population. Theor Appl Genet, 1999, 98: 903–912

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