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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1549-1556.doi: 10.3724/SP.J.1006.2014.01549

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning, Expression, and Functional Analysis of an A Subfamily bZIP Transcription Factor Gene ZmbZIP81 in Maize

WANG Ce1,2,YANG Yan-Ge2,3,LÜ Wei-Tao2,*,ZHOU Chun-Ju1,*,SUN Dong-Mei3,DENG Xin2   

  1. 1 College of Life Science, Northwest Agriculture & Forestry University, Yangling 712100, China; 2 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2014-01-21 Revised:2014-06-16 Online:2014-09-12 Published:2014-06-27
  • Contact: 吕维涛, E-mail: wtlv@ibcas.ac.cn, Tel: 13811773675; 周春菊, E-mail: zhchju@yahoo.com.cn

Abstract:

Basic leucine zipper (bZIP) proteins constitute a large family of transcription factors among eukaryotes, which plays important roles in gene expression regulation under abiotic stress in plants. In order to gain a better understanding of bZIPs in maize, we cloned ZmbZIP81 from maize, which encodes an A subfamily bZIP transcription factor. ZmbZIP81 is located on chromosome 6. The coding region of ZmbZIP81 is 2492 bp in length with four exons and three introns, which encodes 254 animo acid residues. Further study showed that the expression of ZmbZIP81 was induced by exogenous ABA, salt and drought treatments. The transgenic Arabidopsis plants overexpressing ZmbZIP81 were less sensitive to ABA, but more tolerant to salt, compared with the wild type. These results indicated that ZmbZIP81 may encode a transcription factor, regulate ABA signaling negatively and participate in abiotic stress tolerance regulation in maize.

Key words: Maize, bZIP transcription factor, A subfamily, ABA, Abiotic stress

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