Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1686-1694.doi: 10.3724/SP.J.1006.2014.01686

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Nitrogen Fertilization Timing on Lignin Synthesis of Stem and Physiological Mechanism of Lodging Resistance in Wheat 

LU Kun-Li,YIN Yan-Ping,WANG Zhen-Lin*,LI Yong,PENG Dian-Liang,YANG Wei-Bing,CUI Zheng-Yong,YANG Dong-Qing,JIANG Wen-Wen   

  1. National Key Laboratory of crop Biology/ Agronomy College, Shandong Agricultural University, Tai’an 271018, China?
  • Received:2013-11-25 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-10
  • Contact: 王振林, E-mail: zlwang@sdau.edu.cn, Tel: 0538-8241359

Abstract:

For understanding the effects of nitrogen management on lignin synthesis in wheat stem and plant lodging resistance, a field experiment was conducted, using the lodging resistant cultivar Jimai 22 and the lodging sensitive cultivar Shannong 16, in 2011–2012 and 2012–2013 growing seasons. The lignin accumulation in stem, activities of enzymes related to lignin synthesis, lodging resistance index, and grain yield were evaluated under four nitrogen treatments. All treatments received 240 kg ha–1 nitrogen during the whole growth period, in which 80 kg ha−1 was applied before sowing. The remaining nitrogen was applied at pseudo stem erection and booting stages (1:1) for N1, at jointing stage for N2, at jointing and anthesis stages (1:1) for N3, and at booting stage for N4 treatment. Compared with N2 and N3, N1 and N4 were superior in lignin content, stem mechanical strength, and lodging resistance. Nitrogen fertilization timing had significant effects on activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and peroxidase (POD). Under N1 condition, the three key enzymes were in high activity. PAL, TAL and POD activities were higher in N2 treatment than in other treatments at 0–7 d after emergence of the second internode (ESI), and dropped rapidly at 21 d after ESI. Under N3 condition, the PAL, TAL and POD activities were low at 0–21 d after ESI, and the activity decline was alleviated by the nitrogen supplement at anthesis stage (21 d after ESI). Under N4 condition, the activities of these enzymes were increased after 14 d of ESI. At milk and dough stages, both cultivars had higher snapping resistance of the basal stem in N1 and N4 than in N2 and N3 treatments. Besides, the lodging resistance index and grain yield were also significantly higher in N1 and N4 than in N2 and N3. These results indicated that nitrogen applied at booting stage (N1 and N4 treatments) is propitious to increase PAL, TAL, POD activities and lignin content in stem, leading to the improved stem mechanical strength and decreased lodging area and lodging degree of wheat.

Key words: Wheat, Nitrogen management, Enzyme activity, Lignin, Lodging resistance, Yield

[1]罗茂春, 田翠婷, 李晓娟, 林金星. 水稻茎秆形态结构特征和化学成分与抗倒伏关系综述. 西北植物学报, 2007, 27: 2346–2353



Luo M C, Tian C T, Li X J, Lin J X. Relationship between morpho-anatomical traits together with chemical components and lodging resistance of stem in rice. Acta Bot. Bor-Occid.Sin, 2007, 27: 2346–2353 (in Chinese with English abstract)



[2]杨世民, 谢力, 郑顺林, 李静, 袁继超. 氮肥水平和栽插密度对杂交稻茎秆理化特性与抗倒伏性的影响. 作物学报, 2009, 35: 93–103



Yang S M, Xie L, Zheng S L, Li J, Yuan J C. Effects of nitrogen rate and transplanting density on physical and chemical characteristics and lodging resistance of culms in hybrid rice. Acta Agron Sin, 2009, 35: 93–103 (in Chinese with English abstract)



[3]井长勤, 周忠, 张永. 氮肥运筹对小麦倒伏影响的研究. 徐州师范大学学报, 2003, 21(4): 46–49



Jing C Q, Zhou Z, Zhang Y. Effects of Nitrogenous fertilizer application lodging on winter wheat. J Xuzhou Normal Univ, 2003, 21(4): 46–49 (in Chinese)



[4]张睿, 黄历. 小麦生产中倒伏原因分析及解决对策. 陕西农业科学, 2001, (5): 39



Zhang R, Huang L. Analysis of lodging cause and counter measures in wheat production. Shaanxi J Agric Sci, 2001, (5): 39 (in Chinese)



[5]Zahour A, Rasmusson D C, Gallagher L W. Effects of semi-dwarf stature, head number, and kernel number on grain yield in barley in morocco. Crop Sci, 1987, 27: 161–165



[6]Wiersma D W, Oplinger E S, Guy S O. Environment and cultivar effects on winter wheat response to ethephon plant growth regulator. Agron J, 1986, 78: 761–764



[7]肖世和, 张秀英, 闫长生, 张文祥, 海林, 郭慧君. 小麦茎秆强度的鉴定方法研究. 中国农业科学, 2002, 35: 7–11



Xiao S H, Zhang X Y, Yan C S, Zhang W X, Hai L, Guo H J. Determination of resistance to lodging by stem strength in wheat. Sci Agri Sin, 2002, 35: 7–11 (in Chinese with English abstract )



[8]李金才, 尹钧, 魏凤珍. 播种密度对冬小麦茎秆形态特征和抗倒指数的影响. 作物学报, 2005, 31: 662–666



Li J C, Yin J, Wei F Z. Effects of planting density on characters of culm and culm lodging resistant index in winter wheat. Acta Agron Sin, 2005, 31: 662–666 (in Chinese with English abstract)



[9]魏凤珍, 李金才, 王成雨, 屈会娟, 沈学善. 氮肥运筹模式对小麦茎秆抗倒性能的影响. 作物学报, 2008, 34: 1080–1085



Wei F Z, Li J C, Wang C Y, Qu H J, Shen X S. Effects of nitrogenous fertilizer application model on culm lodging resistance in winter wheat. Acta Agron Sin, 2008, 34: 1080–1085 (in Chinese with English abstract)



[10]Turner S R, Somerville C R. Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell, 1997, 9: 689–701



[11]Lewis N G, Yamamoto E. Lignin: occurrence, biogenesis and biodegradation. Annu Rev Plant Physiol Plant Mol Biol, 1990, 41: 455–496



[12]Welton F A. Lodging in oats and wheat. Bot Gazatte, 1928, 85: 121



[13]Tripathi S C, Sayre K D, Kaul J N. Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: effects of genotypes, N levels and ethephon. Field Crops Res, 2003, 84: 271–290



[14]陈晓光, 史春余, 尹燕枰, 王振林, 石玉华, 彭佃亮, 倪英丽, 蔡铁. 小麦茎秆木质素代谢及其与抗倒性的关系. 作物学报, 2011, 37: 1616–1622



Chen X G, Shi C Y, Yin Y P, Wang Z L, Shi Y H, Peng D L, Ni Y L, Cai T. Relationship between lignin metabolism and lodging resistance in Wheat. Acta Agron Sin, 2011, 37: 1616–1622 (in Chinese with English abstract)



[15]董琦, 王爱萍, 梁素明. 小麦基部茎节形态结构特征与抗倒性的研究. 山西农业大学学报, 2003, 23: 188–191



Dong Q, Wang A P, Liang S M. Study on the architectural characteristics of wheat stalks. J Shanxi Agric Univ, 2003, 23: 188–191 (in Chinese with English abstract)



[16]马跃芳, 金晓平, 龚辉. 大麦倒伏原因的初步研究. 作物研究, 1990, 4(4): 22–25



Ma Y F, Jin X P, Gong H. Preliminary study of barley lodging. Crop Res, 1990, 4(4): 22–25 (in Chinese)



[17]徐本生. 施用氮肥与小麦倒伏的关系. 土壤, 1961, (8): 53–54



Xu B S. The relationship between nitrogen application and wheat lodging. Soils, 1961, (8): 53–54 (in Chinese)



[18]李友军, 傅国占, 王绍中, 郭天财. 高产地小麦前氮后移施肥技术. 作物杂志, 1997, (3): 15–16



Li Y J, Fu G Z, Wang S Z, Guo T C. Nitrogenous fertilizer retroposition technology in high yielding fields. Crops, 1997, (3): 15–16 (in Chinese)



[19]陈晓光, 石玉华, 王成雨, 尹燕枰, 宁堂原, 史春余, 李勇, 王振林. 氮肥和多效唑对小麦茎秆木质素合成的影响及其与抗倒性的关系. 中国农业科学, 2011, 44: 3529–3536



Chen X G, Shi Y H, Wang C Y, Yin Y P, Ning T Y, Shi C Y, Li Y, Wang Z L. Effects of nitrogen and PP333 application on the lignin synthesis of stem in relation to lodging resistance of Wheat. Sci Agric Sin, 2011, 44: 3529–3536 (in Chinese with English abstract)



[20]Zadoks J C, Chang T T, Konzak C F. A decimal code for the growth stages of cereals. Weed Res, 1974, 14: 415–421



[21]Bhaskara Reddy M V, Arul J, Angers P, Couture L. Chitosan treatment of wheat seeds induces resistance to Fusarium graminearum and improves seed quality. J Agric Food Chem, 1999, 47: 1208-1216



[22]张志良, 瞿伟. 植物生理学实验指导. 北京: 高等教育出版社, 2003. pp 277–278



Zhang Z L, Qu W. Experimental Guide for Plant Physiology. Beijing: Higher Education Press, 2003. pp 277–278 (in Chinese)



[23]Kofalvi S A, Nassuth A. Influence of wheat streak mosaic virus infection on phenylpropanoid metabolism and the accumulation of phenolics and lignin in wheat. Physiol Mol Plant P, 1995, 47: 365–377



[24]Klapheck S, Zimmer I, Cosse H. Scavenging of hydrogen peroxide in the endosperm of ricinus communis by ascorbate peroxidase. Plant Cell Physiol, 1990, 31: 1005–1013



[25]石海燕, 张玉星. 木质素生物合成途径中关键酶基因的分子特征. 中国农学通报, 2011, 27(5): 288–291



Shi H Y, Zhang Y X. Molecular characterization of key enzyme genes related to the pathway of lignin biosynthesis. Chin Agric Sci Bull, 2011, 27(5): 288–291 (in Chinese with English abstract)



[26]Ookawa T, Ishihara K V. Difference of the cell wall components affecting the ding stress of the culm in relating to the lodging resistance in paddy rice. Jpn J Crop Sci, 1993, 62: 378–384



[27]Strivastava L M. Histochemical studies on lignin. Methods Enzymol, 1996, 49: 173–183



[28]Kokubo A, Sakurai N, Kuraishi S, Takeda K. Culm brittleness of barley (Hordeum vulgar L.) mutants is caused by smaller number of cellulose molecules in cell wall. Plant Physiol, 1991, 97: 509–514



[29]Updegraff D M. Semimicro determination of cellulose in biological materials. Anal Biochem, 1969, 32: 420–424



[30]解新明, 赵燕慧, 霍松, 陈慧. 象草不同品种木质素合成关键酶活性的动态变化. 草地学报, 2010, 18: 523–527



Xie X M, Zhao Y H, Huo S, Chen H. Dynamic changes of Enzyme activities related to lignin biosynthesis for elephant grass cultivars. Acta Agrestia Sin, 2010, 18: 523–527 (in Chinese with English abstract)



[31]Sewalt V J H, Ni W, Blount J W, Jung H G, Masoud S A, Howles P A, Lamb C, Dixon R A. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonialyase or cinnamate 4-hydroxylase. Plant Physiol, 1997, 115: 41–50



[32]刘晓燕, 金继运, 何萍, 高伟, 李文娟. 氯化钾对玉米木质素代谢的影响及其与茎腐病抗性的关系. 中国农业科学, 2007, 40: 2780–2787



Liu X Y, Jin J Y, He P, Gao W, Li W J. Effect of potassium chloride on lignin metabolism and its relation to resistance of corn to stalk rot. Sci Agric Sin, 2007, 40: 2780–2787 (in Chinese with English abstract)



[33]王群瑛, 胡昌浩. 玉米茎秆抗倒特性的解剖研究. 作物学报, 1991, 17: 70–75



Wang Q Y, Hu C H. Studies on the anatomical structures of the stalks of maize with different resistance to lodging. Acta Agron Sin, 1991, 17: 70–75 (in Chinese)



[34]薛应钰, 师桂英, 徐秉良, 陈荣贤. 美洲南瓜(Cucurbita pepo)种皮发育形态观察及其相关酶活性测定. 草业学报, 2011, 20(2): 23–30



Xue Y Y, Shi G Y, Xu B L, Chen R X. Morphological observations on episperm development and related enzyme activity determination in Cucurbita pepo. Acta Pratac Sin, 2011, 20(2): 23–30 (in Chinese)



[35]王晨阳, 朱云集, 夏国军, 宋家永, 李九星, 王永华, 罗毅. 氮肥后移对超高产小麦产量及生理特性的影响. 作物学报, 1998, 24: 978–983



Wang C Y, Zhu Y J, Xia G J, Song J Y, Li J X, Wang Y H, Luo Y. Influence of Nitrogen retroposition on wheat yield and physiological characteristics. Acta Agron Sin, 1998, 24: 978–983 (in Chinese)

[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] WANG Dan, ZHOU Bao-Yuan, MA Wei, GE Jun-Zhu, DING Zai-Song, LI Cong-Feng, ZHAO Ming. Characteristics of the annual distribution and utilization of climate resource for double maize cropping system in the middle reaches of Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(6): 1437-1450.
[3] WANG Wang-Nian, GE Jun-Zhu, YANG Hai-Chang, YIN Fa-Ting, HUANG Tai-Li, KUAI Jie, WANG Jing, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong. Adaptation of feed crops to saline-alkali soil stress and effect of improving saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(6): 1451-1462.
[4] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[5] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[6] CHEN Jing, REN Bai-Zhao, ZHAO Bin, LIU Peng, ZHANG Ji-Wang. Regulation of leaf-spraying glycine betaine on yield formation and antioxidation of summer maize sowed in different dates [J]. Acta Agronomica Sinica, 2022, 48(6): 1502-1515.
[7] LI Yi-Jun, LYU Hou-Quan. Effect of agricultural meteorological disasters on the production corn in the Northeast China [J]. Acta Agronomica Sinica, 2022, 48(6): 1537-1545.
[8] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[9] SHI Yan-Yan, MA Zhi-Hua, WU Chun-Hua, ZHOU Yong-Jin, LI Rong. Effects of ridge tillage with film mulching in furrow on photosynthetic characteristics of potato and yield formation in dryland farming [J]. Acta Agronomica Sinica, 2022, 48(5): 1288-1297.
[10] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[11] YAN Xiao-Yu, GUO Wen-Jun, QIN Du-Lin, WANG Shuang-Lei, NIE Jun-Jun, ZHAO Na, QI Jie, SONG Xian-Liang, MAO Li-Li, SUN Xue-Zhen. Effects of cotton stubble return and subsoiling on dry matter accumulation, nutrient uptake, and yield of cotton in coastal saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(5): 1235-1247.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] LI Rui-Dong, YIN Yang-Yang, SONG Wen-Wen, WU Ting-Ting, SUN Shi, HAN Tian-Fu, XU Cai-Long, WU Cun-Xiang, HU Shui-Xiu. Effects of close planting densities on assimilate accumulation and yield of soybean with different plant branching types [J]. Acta Agronomica Sinica, 2022, 48(4): 942-951.
[14] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[15] DU Hao, CHENG Yu-Han, LI Tai, HOU Zhi-Hong, LI Yong-Li, NAN Hai-Yang, DONG Li-Dong, LIU Bao-Hui, CHENG Qun. Improving seed number per pod of soybean by molecular breeding based on Ln locus [J]. Acta Agronomica Sinica, 2022, 48(3): 565-571.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!