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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 66-71.doi: 10.3724/SP.J.1006.2015.00066


Cloning and Expression Analysis of Ubiquitin-Related Protein Gene BrRUB1 in Brassica rapa ssp. rapifera Tsuda

YAN Hai-Fang*,WANG Fei,LI Qiao, I Hong-Chang,YANG Hu-Cheng   

  1. College of Life Science, Northeast Forestry University, Harbin 150040, China
  • Received:2014-06-20 Revised:2014-09-30 Online:2015-01-12 Published:2014-11-11
  • Contact: 闫海芳, E-mail: icyhf@yahoo.com


RUB1 [related to ubiquitin 1) is a ubiquitin-like protein in plant and yeast, belong to Cullin family. To elucidate the expression feature of RUB1 in Tsuda, we isolated cDNA of RUB1 gene from this plant and named BrRUB1 [GenBank accession No. KF501173). cDNA of BrRUBI was 670 bp in full length and its open reading frame [ORF) was 471 bp in full length, encoding 157 amino acids. BrRUB1-GFP was localized to nucleus, indicating that BrRUB1 may play an important role in the nucleus. Quantitative-PCR analysis showed that BrRUB1 expressed highly in bud and less in petal, showing tissue specificity. Furthermore, the expression of BrRUB1 was induced by UV-A light in the swollen hypocotyls.

Key words: Brassica rapa Tsuda, RUB1, Gene cloning, Expression analysis

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