Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (07): 1047-1055.doi: 10.3724/SP.J.1006.2015.01047

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Changes of DNA Methylation Levels and Patterns in Tea Plant (Camellia sinensis) during Cold Acclimation

ZHOU Yan-Hua,CAO Hong-Li,YUE Chuan,WANG Lu,HAO Xin-Yuan,WANG Xin-Chao*,YANG Ya-Jun*   

  1. Tea Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture / National Center for Tea Improvement, Hangzhou 310008, China
  • Received:2015-01-15 Revised:2015-04-02 Online:2015-07-12 Published:2015-05-04
  • Contact: 王新超, E-mail: wangxinchao@caas.cn; 杨亚军, E-mail: yjyang@tricaas.com E-mail:zhouyh@tricaas.com

Abstract:

Low temperature is one of the most critical environmental factors that limit tea plant growth, survival and geographical distribution. Tea plant can enhance its cold tolerance after undergoing a period of cold acclimation. DNA methylation is one of the epigenetic phenomena, and can be altered by environmental stress in plant. In order to explore the relationship between DNA methylation and low temperature stress response in tea plant, methylation sensitive amplified polymorphism (MSAP) and high performance liquid chromatography (HPLC) were used to analyze the changes of DNA methylation level and pattern in this study. The MSAP results showed that 905, 968, and 970 methylated bands were amplified with 50 selected primers in non-acclimated sample (CK), fully acclimated and de-acclimated samples, with the methylation levels of 50.6%, 54.1%, and 54.2%, respectively. DNA methylation levels in fully acclimated and de-acclimated samples were increased compared with CK. HPLC results were similar with MSAP results. In addition, DNA demethylation and methylation were both occurred during cold acclimation, but the DNA methylation was increased more than the

Key words: Tea plant, Cold acclimation, DNA methylation, MSAP, HPLC

[1]虞富莲. 论茶树原产地和起源中心. 茶叶科学, 1986, 6: 1–8



Yu F L. Discussion on the originating place and the originating centre of tea plant. J Tea Sci, 1986, 6: 1–8 (in Chinese with English abstract)



[2]王新超, 杨亚军. 茶树抗性育种研究现状. 茶叶科学, 2003, 23: 94–98



Wang X C, Yang Y J. Research progress on resistance breeding of tea plant. J Tea Sci, 2003, 23: 94–98 (in Chinese with English abstract)



[3]杨亚军, 郑英雷, 王新超. 冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响. 茶叶科学, 2004, 24: 177–182



Yang Y J, Zheng L Y, Wang X C. Effect of cold acclimation and ABA on cold hardiness, contents of proline of tea plants [Camellia sinensis (L.) O. Kuntze]. J Tea Sci, 2004, 24: 177–182 (in Chinese with English abstract)



[4]杨亚军, 郑雷英, 王新超. 低温对茶树叶片膜脂脂肪酸和蛋白质的影响. 亚热带植物科学, 2005, 34(1): 5–9



Yang Y J, Zheng L Y, Wang X C. Changes of membrane fatty acid composition and protein in tea leaves at low temperature. Subtrop Plant Sci, 2005, 34(1): 5–9 (in Chinese with English abstract)



[5]Wang X C, Zhao Q Y, Ma C L, Zhang Z H, Cao H L, Kong Y M, Yue C, Hao X Y, Chen L, Ma J Q, Jin J Q, Li X, Yang Y J. Global transcriptome profiles of Camellia sinensis during cold acclimation. BMC Genom, 2013, 14: 415



[6]曹红利, 岳川, 郝心愿, 王新超, 杨亚军. 茶树胆碱单加氧酶CsCMO的克隆及甜菜碱合成关键基因的表达分析. 中国农业科学, 2013, 46: 3087–3096



Cao H L, Yue C, Hao X Y, Wang X C, Yang Y J. Cloning of choline monooxygenase (CMO) gene and expression analysis of the key glycine betaine biosynthesis-related genes in tea plant (Camellia sinensis). Sci Agric Sin, 2013, 46: 3087–3096 (in Chinese with English abstract)



[7]曹红利, 岳川, 周艳华, 王璐, 郝心愿, 杨亚军, 王新超. 茶树bZIP 转录因子基因CsbZIP1 的克隆与表达定位. 作物学报, 2014, 40: 1702–1709



Cao H L, Yue C, Zhou Y H, Wang L, Hao X Y, Yang Y J, Wang X C. Molecular cloning and expression of a bZIP transcription factor gene CsbZIP1 in tea plant (Camellia sinensis). Acta Agron Sin, 2014, 40: 1702–1709 (in Chinese with English abstract)



[8]岳川, 曹红利, 周艳华, 王璐, 郝心愿, 王新超, 杨亚军. 茶树谷胱甘肽还原酶基因CsGRs的克隆与表达分析. 中国农业科学, 2014, 47: 3277–3289



Yue C, Cao H L, Zhou Y H, Wang L, Hao X Y, Wang X C, Yang Y J. Cloning and expression analysis of glutathione reductase genes (CsGRs) in tea plant (Camellia sinensis). Sci Agric Sin, 2014, 47: 3277–3289 (in Chinese with English abstract)



[9]Bender J. DNA methylation and epigenetics. Annu Rev Plant Biol, 2004, 55: 41–68



[10]Vanyushin B F, Ashapkin V V. DNA methylation in higher plants: past, present and future. Biochim Biophys Acta, 2011, 1809: 360–368



[11]Chan S W, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat Rev Genet, 2005, 6: 351–360



[12]Wang W, Zhao X, Pan Y, Zhu L, Fu B, Li Z. DNA methylation changes detected by methylation-sensitive amplified polymorphism in two contrasting rice genotypes under salt stress. J Genet Genom, 2011, 38: 419–424



[13]Jullien P E, Kinoshita T, Ohad N, Berger F. Maintenance of DNA methylation during the Arabidopsis life cycle is essential for parental imprinting. Plant Cell, 2006, 18: 1360–1372



[14]Adams K L, Percifield R, Wendel J F. Organ-specific silencing of duplicated genes in a newly synthesized cotton allotetraploid. Genetics, 2004, 168: 2217–2226



[15]潘雅姣, 傅彬英, 王迪, 朱苓华, 黎志康. 水稻干旱胁迫诱导DNA甲基化时空变化特征分析. 中国农业科学, 2009, 42: 3009–3018



Pan Y J, Fu B Y, Wang D, Zhu L H, Li Z K. Spatial and temporal profiling of DNA methylation induced by drought stress in rice. Sci Agric Sin, 2009, 42: 3009–3018 (in Chinese with English abstract)



[16]Steward N, Ito M, Yamaguchi Y, Koizumi N, Sano H. Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. J Biol Chem, 2002, 277(40): 37741–37746



[17]高桂珍, 应菲, 陈碧云, 李浩, 吕晓丹, 闫贵欣, 许鲲, 伍晓明. 热胁迫过程中白菜型油菜种子DNA的甲基化. 作物学报, 2011, 37: 1597–1604



Gao G Z, Ying F, Chen B Y, Li H, Lv X D, Yan G X, Xu K, Wu X M. DNA methylation of seed in response to heat stress in Brassica rapa L. Acta Agron Sin, 2011, 37: 1597–1604 (in Chinese with English abstract)



[18]Dowen R H, Pelizzola M, Schmitz R J, Lister R, Dowen J M, Nery J R, Dixon J E, Ecker J R. Widespread dynamic DNA methylation in response to biotic stress. Proc Natl Acad Sci USA, 2012, 109: E2183–2191



[19]Portis E, Acquadro A, Comino C, Lanteri S. Analysis of DNA methylation during germination of pepper (Capsicum annuum L.) seeds using methylation-sensitive amplification polymorphism (MSAP). Plant Sci, 2004, 166: 169–178



[20]Banaei Moghaddam A M, Fuchs J, Czauderna T, Houben A, Mette M F. Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels. Theor Appl Genet, 2010, 120: 215–226



[21]Sha A H, Lin X H, Huang J B, Zhang D P. Analysis of DNA methylation related to rice adult plant resistance to bacterial blight based on methylation-sensitive AFLP (MSAP) analysis. Mol Genet Genomics, 2005, 273: 484–490



[22]Tan M P. Analysis of DNA methylation of maize in response to osmotic and salt stress based on methylation-sensitive amplified polymorphism. Plant Physiol Biochem, 2010, 48: 21–26



[23]Osabe K, Clement J D, Bedon F, Pettolino F A, Ziolkowski L, Llewellyn D J, Finnegan E J, Wilson I W. Genetic and DNA methylation changes in cotton (Gossypium) genotypes and tissues. PLoS One, 2014, 9(1): e86049



[24]Yaish M W, Peng M, Rothstein S J. Global DNA methylation analysis using methyl-sensitive amplification polymorphism (MSAP). Methods Mol Biol, 2014, 1062: 285–298



[25]Johnston J W, Harding K, Bremner D H, Souch G, Green J, Lynch P T, Grout B, Benson E E. HPLC analysis of plant DNA methylation: a study of critical methodological factors. Plant Physiol Biochem, 2005, 43: 844–853



[26]Zhao Y L, Yu S X, Ye W W, Wang H M, Wang J J, Fang B X. Study on DNA cytosine methylation of cotton (Gossypium hirsutum L.) genome and its implication for salt tolerance. Agric Sci China, 2010, 9: 783–791



[27]Chakrabarty D, Yu K W, Paek K Y. Detection of DNA methylation changes during somatic embryogenesis of Siberian ginseng (Eleuterococcus senticosus). Plant Sci, 2003, 165: 61–68



[28]Richards E J. DNA methylation and plant development. Trends Genet, 1997, 13: 319–323



[29]李雪林, 林忠旭, 聂以春, 郭小平, 张献龙. 盐胁迫下棉花基因组DNA表观遗传变化的MSAP分析. 作物学报, 2009, 35: 588–596



Li X L, Li Z X, Nie Y C, Guo X P, Zhang X L. MSAP analysis of epigenetic changes in cotton (Gossypium hirsutum L.) under salt stress. Acta Agron Sin, 2009, 35: 588–596 (in Chinese with English abstract)



[30]Cervera M T, Ruiz-Garcia L, Martinez-Zapater J M. Analysis of DNA methylation in Arabidopsis thaliana based on methylation-sensitive AFLP markers. Mol Genet Genom, 2002, 268: 543–552



[31]盖树鹏, 张风, 张玉喜, 郑国生. 低温解除牡丹休眠进程中基因组DNA甲基化敏感扩增多态性(MSAP)分析. 农业生物技术学报, 2012, 20: 261–267



Gai S P, Zhang F, Zhang Y X, Zheng G S. Analysis of genomic DNA methylation during chilling induced endo-dormancy release by methylation sensitive amplified polymorphism (MSAP) technology in tree peony (Paeonia suffruticosa). J Agric Biotech, 2012, 20: 261–267 (in Chinese with English abstract)



[32]张勇, 邓科君, 张韬, 彭金华, 周建平, 任正隆. 黑麦基因组DNA甲基化修饰位点的MSAP分析. 麦类作物学报, 2009, 29: 559–564



Zhang Y, Deng K J, Zhang T, Peng J H, Zhou J P, Ren Z L. Analysis on genomic DNA methylation modification of rye by methylation-sensitive ampl ification polymorphism. J Triticeae Crops, 2009, 29: 559–564 (in Chinese with English abstract)



[33]Kalberer S R, Wisniewski M, Arora R. Deacclimation and reacclimation of cold-hardy plants: current understanding and emerging concepts. Plant Sci, 2006, 171: 3–16



[34]Thomashow M F. Plant cold acclimation:Freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 571–599



[35]Christov N.K, Yoneyama S, Shimamoto Y, Imai R. Differential expression of wheat genes during cold acclimation. Cytol Genet, 2007, 41: 142–150



[36]Burn J E, Bagnall D J, Metzger J D, Dennis E S, Peacock W J. DNA methylation, vernalization, and the initiation of flowering. Proc Natl Acad Sci USA, 1993, 90: 287–291



[37]Zhang L, Wang Y, Zhang X H, Zhang M, Han D G, Qiu C P, Han Z H. Dynamics of phytohormone and DNA methylation patterns changes during dormancy induction in strawberry (Fragaria x ananassa Duch.). Plant Cell Rep, 2012, 31: 155–165



[38]Pan Y J Wang W S, Zhao X Q, Zhu L H, Fu B Y, Li Z K. DNA methylation alterations of rice in response to cold stress. Plant Omics J, 2011, 4: 364–369



[39]Mayer B F, Ali-Benali M A, Demone J, Bertrand A, Charron J. Cold acclimation induces distinctive changes in the chromatin state and transcript levels of COR genes in Cannabis sativa varieties with contrasting cold acclimation capacities. Physiol Plant, 2014: DOI: 10.1111/ppl.12318



[40]徐青, 余云舟, 赵萌, 孙东晓. DNA甲基化在动植物遗传育种中的研究进展. 生物技术通讯, 2011, 2: 113–117



Xu Q, Yu Y Z, Zhao M, Sun D X. Progress of DNA methylation in genetics and breeding of plant and animal. Lett Biotech, 2011, 2: 113–117 (in Chinese with English abstract)

[1] LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng. Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1031-1042.
[2] LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping. Key genes mining of DNA methylation involved in regulating drought stress response in potato [J]. Acta Agronomica Sinica, 2021, 47(4): 599-612.
[3] LI Wei-Tao, GUO Jian-Bin, YU Bo-Lun, XU Si-Liang, CHEN Hai-Wen, WU Bei, GONG Ting-Feng, HUANG Li, LUO Huai-Yong, CHEN Yu-Ning, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, JIANG Hui-Fang. Establishment of HPLC-RID method for the determination of soluble sugars in peanut seed [J]. Acta Agronomica Sinica, 2021, 47(2): 368-375.
[4] LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng. DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf [J]. Acta Agronomica Sinica, 2021, 47(12): 2324-2334.
[5] LI Na-Na, LIU Ying, ZHANG Hao-Jie, WANG Lu, HAO Xin-Yuan, ZHANG Wei-Fu, WANG Yu-Chun, XIONG Fei, YANG Ya-Jun, WANG Xin-Chao. Promoter cloning and expression analysis of the hexokinase gene CsHXK2 in tea plant (Camellia sinensis) [J]. Acta Agronomica Sinica, 2020, 46(10): 1628-1638.
[6] Yi YUAN,Shuang ZHU,Ting-Ting FANG,Jin-Jin JIANG,You-Ping WANG. Analysis of drought resistance and DNA methylation level of resynthesized Brassica napus [J]. Acta Agronomica Sinica, 2019, 45(5): 693-704.
[7] LI Peng-Cheng,BI Zhen-Zhen,LIANG Wen-Jun,SUN Chao,ZHANG Jun-Lian,BAI Jiang-Ping. DNA methylation involved in regulating drought stress response of potato [J]. Acta Agronomica Sinica, 2019, 45(10): 1595-1603.
[8] HAO Xin-Yuan,YUEChuan,TANG Hu,QIAN Wen-Jun,WANG Yu-Chun,WANG Lu, WANG Xin-Chao,YANG Ya-Jun. Cloning of β-amylase Gene (CsBAM3) and ItsExpression ModelResponseto Cold Stress in Tea Plant [J]. Acta Agron Sin, 2017, 43(10): 1417-1425.
[9] CAO Hong-Li,WANG Lu,QIAN Wen-Jun,HAO Xin-Yuan,YANG Ya-Jun,WANG Xin-Chao. Positive Regulation of CsbZIP4 Transcription Factor on Salt Stress Response in Transgenic Arabidopsis [J]. Acta Agron Sin, 2017, 43(07): 1012-1020.
[10] TANG Hu,HAO Xin-Yuan,WANG Lu,XIAO Bin,WANG Xin-Chao,YANG Ya-Jun. Molecular Regulation and Substance Exchange Dynamics at Dormancy and Budbreak Stages in Overwintering Buds of Tea Plant [J]. Acta Agron Sin, 2017, 43(05): 669-677.
[11] CHEN Lin-Bo,XIA Li-Fei,TIAN Yi-Ping,LI Mei,SONG Wei-Xi,LIANG Ming-Zhi,JIANG Chang-Jun. Exploring Sterility Gene from Tea Plant Flower Based on Digital Gene Expression Profiling [J]. Acta Agron Sin, 2017, 43(02): 210-217.
[12] ZHANG Yang,HU Zhong-Ying,ZHAO Yue-Ming,LI Na,XIE Li-Nan. DNA Methylation Dynamic Analysis of Self Compatible Line and Self-Incompatible Line of Brassica oleracea var. acephala at Seed Germination Stage [J]. Acta Agron Sin, 2016, 42(04): 532-539.
[13] XIE Tao,RONG Hao,JIANG Jin-Jin*,KONG Yue-Qin,RAN Li-Ping,WU Jian,WANG You-Ping. Analysis of DNA Methylation Patterns in Resynthesized Brassica napus and Diploid Parents [J]. Acta Agron Sin, 2016, 42(04): 513-524.
[14] ZHOU Tian-Shan,WANG Xin-Chao,YU You-Ben,XIAO Yao,QIAN Wen-Jun,XIAO Bin,YANG Ya-Jun. Correlation Analysis between Total Catechins (or Anthocyanins) and Expression Levels of Genes Involved in Flavonoids Biosynthesis in Tea Plant with Purple Leaf [J]. Acta Agron Sin, 2016, 42(04): 525-531 .
[15] QIAN Wen-Jun,YUE Chuan,CAO Hong-Li,HAO Xin-Yuan,WANG Lu,WANG Yu-Chun,HUANG Yu-Ting,WANG Bo,WANG Xin-Chao,XIAO Bin,YANG Ya-Jun. Cloning and Expression Analysis of a Neutral/alkaline Invertase Gene (CsINV10) in Tea Plant (Camellia sinensis L. O. Kuntze) [J]. Acta Agron Sin, 2016, 42(03): 376-388.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!