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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1112-1121.doi: 10.3724/SP.J.1006.2016.01112

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Identification of the ATHB12 Gene of HD-Zip IFamily in Potato(Solanum tuberosum L.)

WU Liang-Liang,YAO Lei,MA Rui,ZHU Xi,YANG Jiang-Wei,ZHANG Ning*,SI Huai-Jun   

  1. Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science /College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2016-01-19 Revised:2016-05-09 Online:2016-08-12 Published:2016-06-02
  • Contact: 张宁, E-mail: ningzh@gsau.edu.cn, Tel: 0931-7631875 E-mail:1335973527@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31460370), Specialized Research Fund for the Doctoral Program of Higher Education of China (20126202110007), International Science & Technology Cooperation Program of China (0102014DFG31570) and Gansu Key Laboratory of Aridland Crop Science of Gansu Agricultural University (GSCS-2012-02).

Abstract:

HD-Zip I is a class of plant-specific transcription factors, which has an important role in response to adversity stress in plant. A ATHB12 gene of HD-Zip I transcription factors was cloned from potato cultivar Gannongshu2, which contains a 759 bp open reading frame (ORF) encoding a protein of 252 amino acid residues. ATHB12 gene is located on potato chromosome 1, and its promoter region sequence contains cis-acting elements including ABRE, LTRECOREATCOR15, WBOXATNPR1 responsive to abiotic stresses (ABA, temperature, dehydration and salt stress). ATHB12 gene expressed in root, stem and leaf of potato, with the highest expression in the root. qRT-PCR analysis confirmed that the gene was induced by PEG, NaCl and ABA, but repressed by cold treatment. The overexpressed-vector of ATHB12 gene driven by the constitutive promoter CaMV 35S was constructed, and the transgenic plants were obtained using Agrobacterium-mediated transformation system. The malondialdehyde (MDA) content in the transgenic plant leaves was significantly lower (P<0.05), whereas the proline content was significantly higher (P<0.05) than those of non-transgenic control under drought stress. The fresh and dry weight of the transgenic plant root was higher than that of non-transgenic plants. These results showed that ATHB12 gene may be involved in response to stress.

Key words: Potato, HD-Zip, THB12 gene, Root, Malondialdehyde, Proline

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