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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 42-50.doi: 10.3724/SP.J.1006.2017.00042

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of CDK Gene in Sugarcane

SU Ya-Chun**,HUANG Long**,LING Hui,WANG Zhu-Qing,LIU Feng,SU Wei-Hua,HUANG Ning,WU Qi-Bin, GAO Shi-Wu,QUE You-Xiong*   

  1. Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture / Fujian Agriculture and Forestry University / Sugarcane Research & Development Center, China Agricultural Technology System, Fuzhou 350002, China
  • Received:2015-12-31 Revised:2016-07-11 Online:2017-01-12 Published:2016-07-28
  • Contact: 阙友雄, E-mail: queyouxiong@126.com
  • Supported by:

    This work was supported by Natural Science Foundation of Fujian Province, China (2015J06006), the Program for New Century Excellent Talents in Fujian Province University (JA14095), the Program of Introducing International Super Agricultural Science and Technology (948 Program) (2014-S18), and the China Agriculture Research System (CARS-20).

Abstract:

Plant cyclin-dependent kinases (CDKs) are a specific class of serine/threonine protein kinases, having synergy with cyclins, as an important acting factor in the process of cell regulation. In this study, a unigene from previous transcriptome data of sugarcane in response to sorghum mosaic virus (SrMV) infection, which exhibited highly homologous to Sorghum biocolor CDK sequence (GenBank Acc No. XP_002466536.1), was validated by RT-PCR and named as ScCDK (GenBank Acc No. KR258796) with a full-length cDNA of 1799 bp. ScCDK contained a complete open reading frame (65–1603 bp) encoding 512 amino acids, and a typical conservative CDK domain, such as ATP binding site, polypeptide substrate binding site and activation loop. Furthermore, based on the bioinformatics prediction, this soluble protein, which was most probably involved in intermediary metabolism, was located in cell nucleus and had no signal peptide but had more random coil upon secondary structure. Real-time quantitative PCR (RT-qPCR) analysis revealed that the expression of this target gene was tissue-specific, with the highest transcript level in bud, followed by the expression in stem pith, root, leaf sheath and epidermis. Besides, its expression could be up-regulated by the treatments of PEG, NaCl and ABA, with nearly 1.9-fold up-regulation of ScCDK transcript under the stress of ABA, compared with the control. These results suggest that the ScCDK may relate to the response of sugarcane to drought and osmotic stresses, meanwhile, be induced by hormones and involved in cell cycle division.

Key words: Sugarcane, CDK, Homology cloning, Bioinformatics analysis, Real-time PCR

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