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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 811-820.doi: 10.3724/SP.J.1006.2017.00811

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Changes of Genes Related to Brassinosteroid Biosynthesis and Signal Transduction during Potato Storage and Its Effect on Tuber Sprouting

ZOU Xue1,2,DENG Meng-Sheng2,LI Li-Qin2,YU Jin-Long1,DING Fan1,HUANG Xue-Li2,PENG Jie2,SHUAI Yu2,CAI Cheng-Cheng2,WANG Xi-Yao2,*   

  1. 1 Mianyang Academy of Agricultural Sciences, Mianyang 621023, China; 2 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2016-10-06 Revised:2017-01-21 Online:2017-06-12 Published:2017-02-17
  • Contact: 王西瑶, E-mail: wxyrtl@163.com
  • About author:王西瑶, E-mail: wxyrtl@163.com
  • Supported by:

    This study was supported by the Sichuan Potato Innovation Team Program of Chinese Modern Agricultural Industrial Technology System (Agricultural Department of Sichuan Province Document [2014] No. 91), the Breeding Program for Public Welfare of Science & Technology Department of Sichuan Province (2016NYZ0032), and the Innovation Fund of Mianyang Academy of Agricultural Sciences (cxjj462016-2019).

Abstract:

To explore the role of brassinosteroid (BR) in tuber sprouting and establish effective sprouting regulation system for seed potato, three varieties and sprouting-inhibitor treatments were used to analyze expression characteristics of nine genes related to BR synthesis, signal transduction and regulation during storage. Effects of BR analogue 24-epibrassinolide (24-eBL) and gibberellin (GA3) on tuber sprouting were also studied. The transcript levels of four genes involved in BR synthesis increased with prolonging storage time, and the time points at which the expression levels began to increase in variety with short dormancy period were earlier than those in varieties with middle or long dormancy period. The expression patterns of BRI1 and CYCD3 were similar to those of synthetic genes while BSK and TCH4 remained constant in varieties with middle or long dormancy period. Sprouting-inhibitors stimulated transcripts of those genes to elevate in the earlier stage of storage, then rapidly decline and stay low levels. There was no significant expression change of transcription factor BZR1 in varieties and sprouting-inhibitor treatments during storage. BR analogue 24-eBL was favorable to tuber dormancy release but shoot growth. The tuber weight per plant treated with 24-eBL and GA3 mixture increased by 37.92% to 98.41% compared with CK. The conclusion, BR synthesis and its signal transduction are essential physiological processes from dormancy to sprouting in potato tuber. Mixture of 24-eBL and GA3 can facilitate uniformity of seed potato sprouting, stronger growth and better tuberization.

Key words: Potato, Tuber sprouting, Brassinosteroid, Gene expression

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