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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (06): 821-828.doi: 10.3724/SP.J.1006.2017.00821

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Verification of SCAR Marker Linked to Maturity in Tetraploid Potato

LI Xing-Cui,LI Guang-Cun,XU Jian-Fei,DUAN Shao-Guang,BIAN Chun-Song,PANG Wan-Fu,LIU Jie,JIN Li-Ping*   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture, Beijing 100081, China
  • Received:2016-07-03 Revised:2017-01-21 Online:2017-06-12 Published:2017-03-13
  • Contact: 金黎平,E-mail: jinliping@caas.cn E-mail:lixingcui1215@163.com
  • Supported by:

    This study was supported by the National Key Technology Support Program of China (2012BAD02B05) and the China Agriculture Research System (CARS-10).

Abstract:

Maturity is one of the most important quantitative traits in potato. In this study, a maturity linked molecular marker SCAR5-8 was developed using Zhongshu 3, Zhongshu 19 and their F1 segregation population consisting of 221 individuals via reduced-representation sequencing and bulked segregant analysis. A further testing of marker SCAR5-8 was performed using 53 early maturity individuals, 63 late maturity individuals and 70 tetraploid varieties, showing the selection accuracy 87.1% and 81.4% between marker test and phenotyping in segregation population and tetraploid varieties, respectively. Moreover, Pearson’s correlation analysis also showed that the marker SCAR5-8 was significantly correlated to maturity trait in potato. The developed SCAR5-8 marker could be used in marker-assisted selection.

Key words: Potato, Maturity, Marker development, Marker-assisted selection

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