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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1170-1180.doi: 10.3724/SP.J.1006.2017.01170

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Polymorphism Evaluation of EST-SSR Markers in Kenaf

WAN Xue-Bei1,2,LI Dong-Xu1,2,XU Yi1,2,XUJian-Tang1,ZHANG Li-Lan1,2,ZHANGLie-Mei1,LINLi-Hui1,QI Jian-Min1,*,ZHANG Li-Wu1,2,*   

  1. 1 Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Ministry of Education / Fujian Key Laboratory for Crop Breeding by Design / College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-07-04 Revised:2017-04-20 Online:2017-08-12 Published:2017-05-08
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898; 张立武, E-mail: lwzhang@fafu.edu.cn / zhang_liwu@hotmail.com E-mail:524073779@qq.com
  • Supported by:

    This study was supported by the Distinguished Young Research Fund in Fujian Agriculture and Forestry University (xjq201401), the China Agriculture Research System(CARS-19-E06),the Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011), the Introduction Breeding and Varieties Demonstration of Featured Crops between China and Benin (2015I0001), and Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002).

Abstract:

 In this study, 94 SSR primers were developed on the basis of the sequences containing transcription factors from 90175 expressed sequence tags (ESTs) in kenaf. A total of 24 kenaf accessions were used to evaluate the polymorphism of these developed SSRs using 9% polyacrylamide gel electrophoresis. Eighty-five SSR primers (90.4%) were successfully amplified and (ATG)n were the dominant motif. Genetic diversity analysis showed that the genetic similarity coefficient varied from 0.62 to 0.92, indicating an abundant genetic basis among these accessions. These developed EST-SSRs not only enrich molecular markers, but also facilitate genetics and breeding research in kenaf.

Key words: Kenaf (Hibiscus cannabinus), Simple sequence repeat, Genetic diversity, Marker development

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