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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1181-1189.doi: 10.3724/SP.J.1006.2017.01181

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis of Structure and Expression of MeHDZ14 Gene in Cassava

YU Xiao-Ling,RUAN Meng-Bin,WANG Bin,YANG Yi-Ling,WANG Shu-Chang*,PENG Ming*   

  1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, China
  • Received:2016-12-09 Revised:2017-04-20 Online:2017-08-12 Published:2017-05-11
  • Contact: 王树昌, E-mail: wangshuchang2001@163.com; 彭明, E-mail: pengming@itbb.org.cn E-mail:yuxiaoling@itbb.org.cn
  • Supported by:

    This study was supported by the International Science & Technology Cooperation Program of China (31561143012,2013DFA32020) and the National Natural Science Foundation of China (31501378).

Abstract:

HD-Zip family genes play an important role in plant growth and stress response. To reveal the role of MeHDZ14 gene in abiotic stresses (e.g. drought) in cassava, we cloned MeHDZ14 gene by using RT-PCR from cassava cultivar SC124, which was relatively more resistant to drought stress. Bioinformatics methods were used to analyze its structural characteristics, and semi-RT-PCR/qRT-PCR was used to explore its expression patterns in response to abiotic stresses in different plant tissues and varieties. MeHDZ14 has a 726 bp open reading frame, encoding 241 amino acids, and contains the typical HD and ZIP domain. Blastp analysis showed that MeHDZ14 has close genetic relationship with ATHB-7, which is a member of the family I HD-Zip gene. Yeast and subcellular localization test showed that the MeHDZ14 gene is a transcription factor and specifically expresses in the nucleus. Genetic structural variation analysis revealed a total of four mis-sense mutations in eight tested varieties. However, amino acid mutations were not found between wild and cultivated cassavas. This indicates the MeHDZ14 proteins are highly conserved. Semi-RT PCR analysis revealed that MeHDZ14 was specifically expressed in petioles, and induced by drought stress in root and leaf, suggesting that MeHDZ14 plays an important role in the early drought stage. Analysis by qRT-PCR showed that MeHDZ14 gene had different expression levels in different cassava varieties, but the same mode under drought stress and ABA treatment. These data indicate that MeHDZ14 is a member of the ABA pathway responding to drought. Our results showed that MeHDZ14 plays an important role in the molecular pathways of cassava drought resistance, underlining its potential in genetic improvement of cassava drought tolerance.

Key words: Cassava, MeHDZ14, Structure characteristics, Expression analysis

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