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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1205-1215.doi: 10.3724/SP.J.1006.2017.01205

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Screening FagopyrumtararicumGenotypes Tolerant to Low Nitrogen Stress at Seedling Stageand Its Evaluating Indices

ZHANG Chu1,ZHANG Yong-Qing1,2,*,LU Zhi-Juan1,LIU Li-Qin1   

  1. 1College of Life Science, Shanxi Normal University, Linfen 041004, China; 2College of Geographical Science, Shanxi Normal University, Linfen 041004, China
  • Received:2017-01-18 Revised:2017-05-10 Online:2017-08-12 Published:2017-05-19
  • Contact: Zhang yongqing, E-mail: yqzhang208@126.com E-mail:zhangchu0331@126.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31571604).

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Abstract:

Nitrogen (N) deficiency is a major problem for agricultural production in the cold area of Loess Plateau. In this, multiple indices of seedlings, including agronomic traits, physiological characteristics, and N utilization parameters, were measured in a hydroponics experiment with nine F. genotypes. The low-N tolerance was assessedby: calculating subordinatefunction values, and making the comprehensive evaluation after principal component and clustering analyses. Under low-N stress, the restraintdegree was greater forshoots than for roots.Compared with normal-N supply, low-N stress resulted in decreases of plant height, stem diameter, leaf area, shoot dry weight, root average diameter, root volume and root surface area and increases of main root length and root-to-shoot ratio. In addition, the root activity, nitrate reductase (NR) activity and soluble protein content decreased under low-N stress, whereas the activities of superoxide dismutase (SOD)and peroxidase (POD) as well as the contents of malonaldehyde (MDA), soluble sugar and free prolineincreased. The photosynthesis-related indices also changed under low-N stress, showing the decreases of leaf chlorophyll content, Fm and Fv/Fm and the increase ofFo. Theplant N contentand plant N accumulation decreased under low-N condition, however, the plant N utilization efficiency showed obvious increase, owing to the greater decline of N accumulation than that of plant biomass.The 25 single indices were converted intofour independent comprehensive indices (accumulative contribution of 87.44%), with which the nine F.genotypes were classified into high-, medium- and low-tolerance groups.An optimal equation was also set up to evaluate and predict low-N tolerance of F. genotypesthrough stepwise regression with D value as the dependent variable and low-N tolerance index as the independent variable. Finally, eight indices, plant height, stem diameter, leaf area, root-shoot ratio, chlorophyll content, Fm, root activity and plant N utilization efficiency,were selected due to their significant impacts on low-N tolerance. These indices are recommended in quick by screening low-N tolerant F.genotypes.tararicumtararicumtararicumtararicum

Key words: Fagopyrumtararicum, Low nitrogen tolerance, Comprehensiveevaluation, Regression analysis

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