玉米雄穗分枝数主效QTL定位及qTBN5近等基因系构建

doi:10.3724/SP.J.1006.2018.01127

Abstract

Abstract:

Tassel branch number (TBN) is a principal component of maize tassel-related traits important for modern maize breeding and production. To understand the genetic basis of tassel branch number, we adopted 288 markers exhibiting polymorphisms between Zheng 58 and Chang 7-2 to construct the genetic linkage map, and conduct quantitative trait loci (QTLs) analysis using sets of 188 recombinant inbred line (RIL) families derived from the elite maize inbred lines vz. Zheng 58 × Chang 7-2. Five major QTLs controlling tassel branch number within five different chromosomes respectively were validated by using the inclusive composite interval mapping method (ICIM) based on phenotypic data collected in two years. Because qTBN5 located on chromosome bin 5.05 was found to be an important QTL, qTBN5 near-isogenic lines (qTBN5-NILs) were developed by backcrossing and marker-assisted selection, and qTBN5 was further mapped in 13.2 Mb intervals. These findings will advance our understanding of the inheritance basis of TBN, and also facilitate the fine mapping and molecular breeding programs in maize.

Key words: maize, tassel branch number, quantitative trait loci mapping, near-isogenic lines

Zi-Ju DAI,Xin-Tao WANG,Qing YANG,Yan WANG,Ying-Ying ZHANG,Zhang-Ying XI,Bao-Quan LI. Major Quantitative Trait Loci Mapping for Tassel Branch Number and Construction of qTBN5 Near-isogenic Lines in Maize (Zea mays L.)[J].Acta Agronomica Sinica, 2018, 44(8): 1127-1135.

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References 27

[1]	Geraldi I O, Miranda Filho J B, Vencovsky R . Estimates of genetic parameters for tassel characters in maize (Zea mays L.) and breeding perspectives. Maydica, 1985,30:1-14
[2]	Upadyayula N, Silva H S, Bohn M O, Rocheford T . Genetic and QTL analysis of maize tassel and inforescence architecture. Theor Appl Genet, 2006,112:592-606 doi: 10.1007/s00122-005-0133-x pmid: 16395569
[3]	Gue R, Wasson C . Genetic analysis of tassel size and leaf senescence and their relationship with yield in two tropical low land maize populations. Afr Crop Sci J, 1996,4:275-281
[4]	Berke T, Rocheford T . Quantitative trait loci for tassel traits in maize. Crop Sci, 1999,39:1439-1443 doi: 10.2135/cropsci1999.3951439x
[5]	Mickelson S M, Stuber C S, Senior L, Kaeppler S M . Quantitative trait loci controlling leaf and tassel traits in a B73lMo17 population of maize. Crop Sci, 2002,42:1902-1909 doi: 10.2135/cropsci2002.1902
[6]	汤华, 严建兵, 黄益勤, 郑用琏, 李建生 . 玉米5个农艺性状的QTL定位. 遗传学报, 2005,32:203-209
	Tang H, Yan J B, Huang Y Q, Zheng Y L, Li J S . QTL mapping of five agronomic traits in maize. Acta Genet Sin, 2005,32:203-209 (in Chinese with English abstract)
[7]	Upadyayula N, Silva H S, Bohn M O, Rocheford T . Genetic and QTL analysis of maize tassel and inforescence architecture. Theor Appl Genet, 2006,112:592-606 doi: 10.1007/s00122-005-0133-x pmid: 16395569
[8]	高世斌, 赵茂俊, 兰海, 张志明 . 玉米雄穗分枝数与主轴长的QTL鉴定. 遗传, 2007,29:1013-1017 doi: 10.3321/j.issn:0253-9772.2007.08.019
	Gao S B, Zhao M J, Lan H, Zhang Z M . Identification of QTL associated with tassel branch number and total tassel length in maize. Hereditas (Beijing), 2007,29:1013-1017 (in Chinese with English abstract) doi: 10.3321/j.issn:0253-9772.2007.08.019
[9]	王迪, 李永祥, 王阳, 刘成, 刘志斋, 彭勃, 谭巍巍, 张岩, 孙宝成, 石云素, 宋燕春, 王天宇, 黎裕 . 控制玉米雄穗分枝数目和雄穗重的主效QTL的定位. 植物学报, 2001,46:11-20 doi: 10.3724/SP.J.1259.2011.00011
	Wang D, Li Y X, Wang Y, Liu C, Liu Z Z, Peng B, Tan W W, Zhang Y, Sun B C, Shi Y S, Song Y C, Wang T Y, Li Y . Major quantitative trait loci analysis of tassel primary branch number and tassel weight in maize(Zea mays L.). Chin Bull Bot, 46:11-20 (in Chinese with English abstract) doi: 10.3724/SP.J.1259.2011.00011
[10]	Brown P J, Upadyayula N, Mahone G S, Tian F, Bradbury P J, Myles S, Holland J B, Flint-Garcia S, McMullen M M, Buckler E S, Rocheford T R . Distinct genetic architectures for male and female inflorescence traits of maize. PLoS Genet, 2011,7(11):e1002383 doi: 10.1371/journal.pgen.1002383 pmid: 3219606
[11]	杨钊钊, 李永祥, 刘成, 刘志斋, 李春辉, 李清超, 彭勃, 张岩, 王迪, 谭巍巍, 孙宝成, 石云素, 宋燕春, 王天宇, 黎裕 . 基于多个相关群体的玉米雄穗相关性状QTL分析. 作物学报, 2012,38:1435-1442
	Yang Z Z, Li Y X, Liu C, Liu Z Z, Li C H, Li Q C, Peng B, Zhang Y, Wang D, Tan W W, Sun B C, Shi Y S, Song Y C, Wang T Y, Li Y . QTL analysis of tassel-related traits in maize (Zea mays L.) using multiple connected populations. Acta Agron Sin, 2012,38:1435-1442 (in Chinese with English abstract)
[12]	Chen Z L, Wang B B, Dong X M, Liu H, Ren L H, Chen J, Hauck A, Song W B, Lai J S . An ultra-high density bin-map for rapid QTL mapping for tassel and ear architecture in a large F₂ maize population. BMC Genomics, 2014,15:433 doi: 10.1186/1471-2164-15-433
[13]	Wu X, Li Y X, Shi Y S, Song Y C, Zhang D F, Li C H, Buckler E S, Li Y, Zhang Z W, Wang T Y . Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize. Plant Biotechnol J, 2016,14:1551-1562 doi: 10.1111/pbi.2016.14.issue-7
[14]	Xu G H, Wang X F, Huang C, Xu D Y, Li D, Tian J G, Chen Q Y, Wang C L, Liang Y M, Wu Y Y, Yang X H, Tian F . Complex genetic architecture underlies maize tassel domestication. New Phytol, 2017,214:852-864 doi: 10.1111/nph.14400 pmid: 28067953
[15]	Vollbrecht E, Springer P S, Goh L, Buckler E S, Martienssen R . Architecture of floral branch systems in maize and related grasses. Nature, 2005,436:1119-1126 doi: 10.1038/nature03892 pmid: 16041362
[16]	Satoh-Nagasawa N, Nagasawa N, Malcomber S, Sakai H, Jackson D . A trehalose metabolic enzyme controls inflorescence architecture in maize. Nature, 2006,441:227-230 doi: 10.1038/nature04725 pmid: 16688177
[17]	Bortiri E, Chuck G, Vollbrecht E, Rocheford T, Martienssen R, Hake S . ramosa2 encodes a LATERAL ORGAN BOUNDARY domain protein that determines the fate of stem cells in branch meristems of maize. Plant Cell, 2006,18:574-585
[18]	Gallavotti A, Zhao Q, Kyozuka J, Meeley R B, Ritter M K, Doebley J F, Pè M E, Schmidt R J . The role of barren stalk1 in the architecture of maize. Nature, 2004,432:630-635
[19]	Walsh J, Freeling M . The liguleless2 gene of maize functions during the transition from the vegetative to the reproductive shoot apex. Plant J, 1999,19:489-495
[20]	Skirpan A, Culler A H, Gallavotti A, Jackson D, Cohen J D, McSteen P . BARREN INFLORESCENCE2 interaction with ZmPIN1a suggests a role in auxin transport during maize inflorescence development. Plant Cell Physiol, 2009,50:652-657 doi: 10.1093/pcp/pcp006
[21]	Chuck G, Brown P, Meeley R, Hake S . Maize SBP-box transcription factors unbranched2 and unbranched3 affect yield traits by regulating the rate of lateral primordia initiation. Proc Natl Acad Sci USA, 2014,111:18775-18780
[22]	Lander E S, Green P, Abranhanson J, Barlow A, Daley M, Lincoln S, Newburg L . MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987,1:174-181 doi: 10.1016/0888-7543(87)90010-3
[23]	Meng L, Li H H, Zhang L Y, Wang J K . QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in bi-parental populations. Crop J, 2015,3:169-173 doi: 10.1016/j.cj.2015.05.001
[24]	Brewbaker J L . Diversity and genetics of tassel branch numbers in maize. Crop Sci, 2015,55:65-78 doi: 10.2135/cropsci2014.03.0248
[25]	Briggs W H, McMullen M D, Gaut B S, Doebley J . Linkage mapping of domestication loci in a large maize-teosinte backcross resource. Genetics, 2007,177:1915-1928 doi: 10.1534/genetics.107.076497 pmid: 17947434
[26]	Kubo T, Aida Y, Nakamura K, Tsunematsu H, Doi K, Yoshimura A . Reciprocal chromosome segment substitution series derived from japonica and indica cross of rice (Oryza sativa L.). Breed Sci, 2002, 52: 319-325
[27]	Chen Z J, Yang C, Tang D G, Zhang L, Zhang L, Qu J T, Liu J . Dissection of the genetic architecture for tassel branch number by QTL analysis in two related populations in maize. J Integr Agric, 2017,16:1432-1442 doi: 10.1016/S2095-3119(16)61538-1

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年份 Year	郑58 Zheng 58	昌7-2 Chang 7-2	均值 Average	范围 Range	变异系数 Variation coefficient (%)
2015	4.6	17.8	10.7	2.4-22.6	43.60
2016	4.4	17.2	11.2	2.8-21.4	38.83

QTL	染色体 Chromosome	物理区间 Position (Mb)	标记区间 Marker interval	LOD	加性效应 Additive effect	贡献率 R² (%)
qTBN3	3	146.7-175.5	umc1954-bnlg1798	5.06	1.40	13.67
qTBN4	4	169.2-186.7	bnlg2291-umc2041	3.15	-1.01	5.81
qTBN5	5	170.1-200.2	umc2111-bnlg278	9.58	1.88	19.92
qTBN7	7	29.4-83.2	bnlg2233-bnlg1380	4.65	-0.82	16.53
qTBN10	10	127.3-145.5	umc2003-phi323152	6.11	-1.73	9.06

Major Quantitative Trait Loci Mapping for Tassel Branch Number and Construction of qTBN5 Near-isogenic Lines in Maize (Zea mays L.)

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