甘蓝型油菜种子萌发期耐苯磺隆种质筛选与综合评价

doi:10.3724/SP.J.1006.2018.01169

Abstract

Abstract:

The purpose of this study is to explore the characteristics of tribenuron-methyl tolerance in different genotypes of Brassica napus, and establish the evaluation system for screening and breeding new cultivars with tribenuron-methyl tolerance. The germination vigor was measured on the third day after treatment and the germination rate, shoot length, root length, dry weight, as well as fresh weight were measured on the seventh day. The identification of tolerance at germination stage was performed by tribenuron-methyl tolerance comprehensive evaluation value (T), average subordinative function value (ASF) and weight tribenuron-methyl tolerance coefficient (WTC) in correlation analysis, frequency analysis, principal component analysis, grey analysis, cluster analysis and stepwise regression analysis. Based on the variance analysis of relative root length index in 11 tested materials, we determined the optimum treatment concentration (25 mg kg ^-1) for selecting and evaluating the tolerant germplasm resources to tribenuron-methyl in napeseed. Under the optimum concentration, the single factor variance analysis showed significant differences in root length, shoot length, germination rate, germination vigor and fresh weight among 241 accessions of Brassica napus. The clustering analysis according to T value exhibited that the tested cultivars were roughly divided into five tribenuron-methyl tolerance grades, including three in grade I, 30 in grade II, 198 in grade III, six in grade IV, and four in grade V. We conclude that the varieties (lines) with strong tribenuron-methyl tolerance at germination stage are Xiwang 106, SWU95, and WH-33, which could be used as the germplasm materials for the study on tolerance breeding and mechanism. Furthermore, the root length, fresh weight and germination rate could be used as index traits to evaluate the tolerance of rapeseed germplasm resources during germination stage.

Key words: Brassica napus L, Tribenuron-methyl tolerance, germplasm resources, comprehensive evaluation, germination stage

Qian WANG,Cui CUI,Sang YE,Ming-Sheng CUI,Yu-Feng ZHAO,Na LIN,Zhang-Lin TANG,Jia-Na LI,Qing-Yuan ZHOU. Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.)[J].Acta Agronomica Sinica, 2018, 44(8): 1169-1184.

Figures/Tables 10

Table 1

Table 2

Fig. 1

Table 3

Table 4

Table 5

Table 6

ASF value, WTC value, T value, and final rank of 241 accessions of rape germplasm resources under tribenuron-methyl stress"

编号 Code	ASF值 ASF value		WTC值 WTC value		T值 T value		综合排序 Final rank
编号 Code	数值Value	排序Order	数值Value	排序Order	数值Value	排序Order	综合排序 Final rank
1	0.55	35	0.55	30	0.28	73	40
2	0.46	167	0.53	67	-0.30	178	141
3	0.49	127	0.55	32	0.07	108	81
4	0.48	138	0.54	62	-0.16	154	124
5	0.44	192	0.50	164	-0.55	201	192
6	0.57	24	0.55	26	0.65	31	25
7	0.48	141	0.48	194	-0.29	177	175
8	0.42	213	0.46	208	-0.53	199	212
9	0.47	148	0.52	101	-0.29	176	148
10	0.54	49	0.55	33	0.55	35	35
11	0.47	147	0.52	114	-0.14	145	138
12	0.52	78	0.52	121	0.05	111	103
13	0.45	177	0.52	106	-0.62	207	165
14	0.51	84	0.53	84	0.23	80	74
15	0.42	206	0.52	100	-0.66	215	178
16	0.47	150	0.49	174	-0.01	121	153
17	0.47	154	0.50	145	-0.15	151	154
18	0.50	98	0.53	83	0.10	105	85
19	0.42	203	0.49	178	-0.72	218	209
20	0.50	110	0.52	99	0.13	98	100
21	0.45	184	0.50	144	-0.55	203	181
22	0.44	194	0.51	132	-0.52	198	179
23	0.44	196	0.48	183	-0.54	200	202
24	0.49	122	0.53	65	-0.14	146	116
25	0.46	164	0.50	163	-0.12	141	161
26	0.47	155	0.51	123	-0.30	179	158
27	0.40	218	0.46	212	-0.83	227	218
28	0.49	134	0.52	94	0.05	110	118
29	0.60	11	0.57	7	0.79	24	11
30	0.42	209	0.48	191	-0.60	206	210
31	0.52	68	0.54	57	0.02	118	70
32	0.53	58	0.52	98	0.22	81	67
33	0.59	14	0.56	13	0.96	15	12
34	0.48	143	0.52	104	-0.21	163	140
35	0.33	235	0.41	236	-1.28	234	235
36	0.16	241	0.36	241	-2.57	241	241
37	0.50	92	0.50	141	-0.01	119	123
38	0.25	238	0.38	238	-1.77	238	238
39	0.48	140	0.53	66	-0.10	138	122
40	0.47	157	0.54	49	-0.07	133	119
41	0.46	169	0.53	82	-0.43	191	152
42	0.50	107	0.53	63	0.03	116	86
43	0.41	215	0.49	166	-0.57	204	206
编号 Code	ASF值 ASF value		WTC值 WTC value		T值 T value		综合排序 Final rank
编号 Code	数值Value	排序Order	数值Value	排序Order	数值Value	排序Order	综合排序 Final rank
44	0.46	162	0.50	147	-0.13	143	156
45	0.44	197	0.49	175	-0.39	186	194
46	0.49	120	0.54	47	-0.02	122	87
47	0.39	223	0.45	223	-0.74	219	223
48	0.51	89	0.54	40	0.35	64	54
49	0.54	43	0.53	81	0.35	63	52
50	0.53	52	0.55	25	0.21	82	46
51	0.42	204	0.47	206	-0.36	185	208
52	0.64	5	0.57	9	1.13	6	5
53	0.50	106	0.50	156	-0.07	135	134
54	0.50	108	0.51	137	0.36	60	99
55	0.44	187	0.48	186	-0.31	181	191
56	0.46	163	0.49	167	-0.17	157	164
57	0.45	182	0.47	197	-0.24	167	189
58	0.50	103	0.54	58	-0.15	152	106
59	0.46	168	0.48	185	-0.31	180	183
60	0.54	50	0.55	27	0.46	46	37
61	0.47	160	0.53	75	-0.33	182	144
62	0.51	83	0.53	86	0.33	67	66
63	0.32	236	0.42	235	-1.41	236	236
64	0.53	60	0.54	55	0.20	86	58
65	0.54	46	0.54	52	0.53	39	39
66	0.55	34	0.56	14	0.53	38	26
67	0.49	124	0.53	89	-0.27	172	131
68	0.50	104	0.53	73	-0.11	140	107
69	0.45	175	0.52	105	-0.39	188	162
70	0.52	71	0.52	102	0.18	88	78
71	0.39	222	0.44	224	-0.81	225	225
72	0.57	21	0.54	42	1.00	12	20
73	0.29	237	0.41	237	-1.47	237	237
74	0.53	51	0.54	38	0.13	99	53
75	0.45	174	0.54	61	-0.26	169	136
76	0.52	69	0.50	142	0.04	114	112
77	0.51	85	0.51	133	0.12	100	109
78	0.45	173	0.49	181	-0.17	155	172
79	0.52	72	0.52	118	0.16	92	84
80	0.40	219	0.45	222	-0.77	223	221
81	0.37	228	0.42	234	-0.75	221	229
82	0.54	42	0.51	124	0.11	101	82
83	0.42	211	0.47	198	-0.64	212	213
84	0.47	152	0.50	152	0.02	117	147
85	0.49	133	0.50	158	-0.08	136	150
86	0.45	179	0.45	215	-0.10	137	182
87	0.21	239	0.38	239	-1.99	239	239
88	0.56	31	0.54	59	0.44	48	41
89	0.53	63	0.52	96	0.52	40	57
90	0.45	172	0.49	169	-0.15	149	166
91	0.43	202	0.46	209	-0.22	164	201
92	0.50	101	0.53	74	0.32	69	73
93	0.51	90	0.51	139	0.35	61	89
94	0.52	77	0.56	22	0.42	55	45
95	0.47	156	0.49	165	-0.13	144	160
96	0.55	33	0.56	16	0.43	49	31
97	0.52	75	0.53	70	0.04	115	77
98	0.53	54	0.53	76	0.54	37	47
99	0.50	102	0.54	45	0.15	96	71
100	0.38	226	0.45	219	-0.92	231	227
101	0.45	171	0.50	161	-0.43	192	180
102	0.57	25	0.53	72	0.90	17	34
103	0.45	176	0.51	131	-0.35	183	167
104	0.53	62	0.50	143	0.15	95	95
105	0.42	212	0.47	205	-0.68	216	216
106	0.55	36	0.52	110	0.76	26	48
107	0.59	13	0.56	15	0.97	14	13
108	0.50	93	0.53	79	-0.15	150	111
109	0.54	44	0.54	53	0.43	50	44
110	0.51	88	0.51	136	0.20	83	101
111	0.48	137	0.50	148	-0.04	127	142
112	0.49	119	0.54	60	-0.04	129	102
113	0.52	79	0.50	159	0.37	59	91
114	0.52	80	0.53	64	0.20	84	64
115	0.49	125	0.52	97	0.27	75	92
116	0.49	131	0.51	127	-0.19	162	146
117	0.52	74	0.53	85	0.40	57	61
118	0.47	151	0.52	92	-0.16	153	133
119	0.49	121	0.52	117	-0.01	120	127
120	0.45	180	0.49	180	-0.51	197	193
121	0.50	99	0.52	95	0.08	107	96
122	0.42	207	0.47	201	-0.65	213	214
122	0.42	207	0.47	201	-0.65	213	214
123	0.38	225	0.43	227	-0.62	208	219
124	0.47	159	0.50	155	-0.40	189	171
125	0.44	190	0.46	207	-0.19	161	195
126	0.53	65	0.51	140	0.42	54	75
127	0.44	189	0.48	190	-0.63	209	207
128	0.51	87	0.52	109	-0.05	131	114
129	0.39	221	0.44	225	-0.77	224	224
130	0.42	208	0.51	135	-0.44	193	185
131	0.42	210	0.49	176	-0.17	156	188
132	0.40	217	0.46	210	-0.76	222	217
133	0.61	7	0.56	21	0.97	13	10
134	0.54	47	0.54	51	0.51	42	42
135	0.43	200	0.48	195	-0.36	184	203
136	0.41	214	0.45	220	-0.90	230	222
137	0.42	205	0.48	192	-0.71	217	211
138	0.50	111	0.53	88	0.10	102	97
139	0.50	94	0.54	56	-0.11	139	88
140	0.51	82	0.55	34	0.17	91	59
141	0.49	126	0.52	103	0.13	97	113
142	0.58	19	0.55	24	0.72	27	17
143	0.49	123	0.51	128	-0.04	128	129
144	0.50	109	0.53	87	-0.04	125	110
145	0.47	146	0.52	112	-0.17	159	145
146	0.55	39	0.57	10	0.80	22	18
147	0.44	198	0.49	182	-0.55	202	204
148	0.55	37	0.56	17	0.52	41	30
149	0.39	220	0.44	226	-0.81	226	226
150	0.49	129	0.52	116	-0.25	168	143
151	0.50	96	0.50	153	0.08	106	125
152	0.49	132	0.50	162	-0.07	134	151
153	0.50	113	0.52	120	0.07	109	120
154	0.49	130	0.50	154	-0.24	166	155
155	0.43	201	0.45	217	-0.65	214	215
156	0.49	128	0.52	119	0.17	90	117
157	0.59	16	0.57	6	0.66	30	15
158	0.57	20	0.56	23	0.60	33	22
159	0.56	26	0.56	20	0.40	58	32
160	0.50	95	0.54	39	0.10	103	68
161	0.45	183	0.48	184	-0.45	194	198
162	0.63	6	0.56	11	1.25	5	6
163	0.44	195	0.50	149	-0.46	195	187
164	0.56	28	0.55	29	0.89	18	21
165	0.49	117	0.46	211	0.71	28	126
166	0.35	232	0.42	230	-1.29	235	233
167	0.46	170	0.51	130	-0.49	196	170
168	0.49	135	0.53	69	-0.04	126	115
169	0.59	12	0.57	8	0.83	20	9
170	0.53	56	0.54	50	0.30	70	49
171	0.50	114	0.50	150	0.23	79	121
172	0.47	158	0.53	77	-0.26	170	137
173	0.52	73	0.49	173	0.35	65	104
174	0.53	64	0.54	43	0.30	71	50
175	0.51	81	0.51	129	0.43	51	79
176	0.58	18	0.57	5	0.54	36	16
177	0.53	55	0.55	36	0.15	94	51
178	0.49	115	0.52	93	0.10	104	105
179	0.44	191	0.52	108	-0.64	211	173
180	0.68	1	0.58	4	2.16	2	2
181	0.52	66	0.52	107	0.41	56	65
182	0.57	23	0.55	31	0.79	23	23
183	0.52	70	0.51	125	0.32	68	80
184	0.44	193	0.47	200	-0.12	142	184
185	0.68	2	0.60	2	1.12	7	4
186	0.53	53	0.51	126	0.93	16	55
187	0.48	142	0.49	171	-0.26	171	163
188	0.54	45	0.49	179	0.86	19	72
189	0.48	139	0.48	187	-0.05	130	157
190	0.37	229	0.45	221	-1.19	233	230
191	0.17	240	0.37	240	-2.14	240	240
192	0.53	61	0.52	115	0.46	45	63
193	0.38	224	0.43	229	-0.63	210	220
194	0.41	216	0.45	218	-0.14	148	205
195	0.48	145	0.53	78	0.28	74	93
196	0.44	186	0.47	202	-0.28	173	199
197	0.51	86	0.48	188	0.51	43	108
198	0.49	118	0.50	157	0.04	112	132
199	0.67	3	0.62	1	2.21	1	1
200	0.46	165	0.46	213	-0.28	175	190
201	0.50	97	0.52	91	0.18	89	83
202	0.45	178	0.48	193	-0.39	187	196
203	0.60	9	0.56	18	1.03	10	8
204	0.60	8	0.53	68	1.34	4	24
205	0.46	161	0.49	177	-0.28	174	176
206	0.36	230	0.42	233	-0.89	229	232
207	0.59	15	0.56	12	1.04	9	7
208	0.56	30	0.55	37	0.82	21	27
209	0.45	185	0.45	216	-0.23	165	200
210	0.49	116	0.50	146	-0.14	147	139
211	0.59	17	0.56	19	1.01	11	14
212	0.47	149	0.47	203	-0.17	158	174
213	0.47	153	0.46	214	-0.02	123	168
214	0.56	29	0.55	28	0.56	34	28
215	0.67	4	0.60	3	1.85	3	3
216	0.43	199	0.47	199	-0.19	160	197
217	0.36	231	0.43	228	-0.75	220	228
218	0.52	67	0.52	90	0.20	85	69
219	0.50	105	0.51	122	0.29	72	94
220	0.45	181	0.49	168	0.26	76	149
221	0.54	48	0.54	44	0.43	52	43
222	0.50	112	0.49	172	0.24	78	128
223	0.57	22	0.54	46	0.79	25	29
224	0.56	27	0.54	48	0.67	29	33
225	0.50	91	0.51	138	0.35	66	90
226	0.48	136	0.50	151	0.04	113	135
227	0.55	40	0.52	111	0.35	62	60
228	0.48	144	0.48	189	-0.03	124	159
229	0.60	10	0.54	54	1.07	8	19
230	0.52	76	0.51	134	0.16	93	98
231	0.46	166	0.48	196	-0.06	132	169
232	0.44	188	0.50	160	-0.41	190	186
233	0.50	100	0.47	204	0.25	77	130
234	0.53	57	0.49	170	0.63	32	76
235	0.37	227	0.42	231	-0.87	228	231
236	0.55	38	0.55	35	0.48	44	36
237	0.55	32	0.52	113	0.42	53	56
238	0.54	41	0.54	41	0.44	47	38
239	0.53	59	0.53	71	0.19	87	62
240	0.33	234	0.53	80	-0.57	205	177
241	0.34	233	0.42	232	-1.18	232	234

Table 6

Table 7

Table 8

Table 9

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品种(系) Variety (line)	苯磺隆浓度 Concentration of tribenuron-methyl
品种(系) Variety (line)	0.25 mg kg^-1		2.50 mg kg^-1		25.00 mg kg^-1		250.00 mg kg^-1
炎81-2 Yan 81-2	70.3±0.007	c	17.8±0.015	de	10.4±0.003	f	10.3±0.003	de
SWU92	82.5±0.030	b	19.3±0.009	de	14.5±0.002	def	11.7±0.011	de
SWU101	64.5±0.077	cd	15.3±0.017	de	12.5±0.001	ef	9.3±0.012	e
782	48.5±0.003	e	18.5±0.004	de	13.5±0.006	ef	12.9±0.002	cde
509	50.0±0.008	e	19.8±0.021	de	15.4±0.004	cdef	14.5±0.009	bcde
P18	106.3±0.091	a	36.0±0.031	a	31.1±0.002	a	28.8±0.016	a
甲预31棚 Jiayu 31 peng	24.6±0.010	f	22.4±0.013	cd	19.6±0.029	bcd	15.8±0.006	bcde
苏油1号 Suyou 1	95.7±0.039	a	26.1±0.008	bc	23.8±0.012	b	20.0±0.004	bc
至尊 Zhizun	85.2±0.002	b	29.3±0.030	ab	22.3±0.001	bc	21.4±0.019	ab
B265	96.4±0.044	a	31.1±0.054	ab	18.5±0.011	bcde	18.0±0.006	bcd
中双12 Zhongshuang 12	60.1±0.010	d	13.3±0.017	e	13.2±0.038	ef	13.5±0.059	cde

项目 Item	参数 Parameter		根长 RL (cm)	芽长 SL (cm)	发芽势 GV (%)	发芽率 GR (%)	鲜重 FW (g)	干重 DW (g)
对照 Control	最大值 Max.		13.13	5.81	100.00	100.00	0.79	0.08
	最小值 Min.		0.55	0.76	10.00	15.00	0.08	0.01
	均值 Average		7.38^**	2.27^**	81.31^**	87.94^**	0.38^**	0.04^**
	标准差 SE		1.61	1.24	19.87	14.47	0.11	0.01
	变异系数 CV (%)		21.77	54.56	24.44	16.45	28.91	26.74
胁迫 Stress	最大值 Max.		2.97	6.24	100.00	100.00	0.79	0.06
	最小值 Min.		0.34	0.71	5.00	15.00	0.12	0.01
	均值 Average		0.94^**	2.37^**	75.77^**	83.22^**	0.36^**	0.04^**
	标准差 SE		0.38	1.30	21.47	16.66	0.10	0.01
	变异系数 CV (%)		40.00	54.83	28.34	20.01	28.00	25.91
较对照变化		均值 Average	-6.44	0.10	-5.54	-4.72	-0.02	0
Comparison with the control		变异系数 CV (%)	18.23	0.27	3.90	3.56	-0.91	-0.83

参数 Parameter	根长 RL	芽长	发芽势	发芽率	鲜重	干重
参数 Parameter	根长 RL	SL	GV	GR	FW	DW
最大值 Max.	0.588	2.005	1.000	1.000	2.357	1.946
最小值 Min.	0.050	0.494	0.143	0.333	0.411	0.333
均值 Average	0.136^**	1.052^**	0.926^**	0.942^**	0.980^**	1.055
标准差 SE	0.066	0.187	0.123	0.097	0.202	0.136
变异系数 CV (%)	48.950	17.800	13.269	10.276	20.593	12.856

指标 Index	根长 RL	芽长 SL	鲜重 FW	发芽率 GR	发芽势 GV	干重 DW
RL	1
SL	0.046	1
FW	0.042	0.215^**	1
GR	-0.150^*	-0.059	-0.018	1
GV	-0.171^**	0.031	0.022	0.687^**	1
DW	-0.128^*	0.031	0.062	0.133^*	0.097	1

主成分 Principal component	特征值 Eigen value	贡献率 Variance contribution (%)	累计贡献率 Accumulated variance contribution (%)	所测指标的特征向量值 Eigen vector of measured indicators
主成分 Principal component	特征值 Eigen value	贡献率 Variance contribution (%)	累计贡献率 Accumulated variance contribution (%)	发芽率 GR	发芽势GV	鲜重 FW	根长 RL	芽长 SL	干重 DW
1	1.810	30.167	30.167	0.978	0.945	0.279	0.011	0.261	0.617
2	1.310	21.825	51.992	-0.358	-0.263	0.841	0.396	0.769	0.258
3	1.096	18.267	70.259	0.115	0.311	-0.263	0.904	0.195	-0.637
4	0.790	13.159	83.418	-0.044	-0.057	0.316	0.508	-0.755	0.323

Screening and Comprehensive Evaluation of Germplasm Resources with Tribenuron-methyl Tolerance at Germination Stage in Rapeseed (Brassica napus L.)

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指标 Index	等权关联度γ_T		权重系数 ω_i	加权关联度γ_WTC
指标 Index	数值 Value	排序 Order	权重系数 ω_i	数值 Value	排序 Order
发芽率 GR	0.649	3	0.163	0.669	5
发芽势 GV	0.634	4	0.159	0.671	4
鲜重 FW	0.658	2	0.165	0.745	2
干重 DW	0.633	5	0.159	0.615	6
根长 RL	0.781	1	0.196	0.763	1
芽长 SL	0.630	6	0.158	0.673	3

指标 Index	隶属函数值 Subordinate function value
指标 Index	I	II	III	V	IV
发芽率 GR	0.94	0.94	0.93	0.84	0.48
发芽势 GV	0.98	0.96	0.92	0.83	0.43
鲜重 FW	0.71	0.28	0.21	0.16	0.15
干重 DW	0.50	0.43	0.41	0.35	0.22
根长 RL	0.44	0.29	0.15	0.11	0.09
芽长 SL	0.40	0.47	0.40	0.32	0.28
ASF值 ASF value	0.67	0.57	0.50	0.43	0.28
WTC值 WTC value	0.60	0.54	0.52	0.48	0.40
T值 T value	2.08	0.77	0.12	-0.47	-1.63

因变量 Dependent variable	多元逐步回归方程 Multiple stepwise regression equation	相关系数 r	F值 F-value	P值 P-value
ASF值 ASF value	y = -0.4505+0.6436x₁+0.1260x₃+0.4108x₅+0.1421x₆	0.9555	620	0.0001
WTC值 WTC value	y = -0.0506+0.2568x₁+0.1867x₂+0.3401x₃-0.0606x₄+0.0910x₅+0.0320x₆	0.9578	433	0.0001
T值 T value	y = -6.5511+4.5564x₁+1.5790x₃+5.2021x₅	0.9247	466	0.0001

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