大豆引进种质抗胞囊线虫病、抗花叶病毒病和耐盐基因型鉴定及优异等位基因聚合种质筛选

doi:10.3724/SP.J.1006.2018.01263

Abstract

Abstract:

China has introduced 3218 soybean accessions from 26 countries such as the United States, Russia and Japan, and some of them have been carried out soybean cyst nematode (SCN), soybean mosaic virus (SMV) and salinity tolerance resistance evaluation. However, the systematic genotyping of these accessions has not been reported yet. In this study, five robust functional markers have been developed for KASP assays, three SCN loci (rhg1, Rhg4, SCN3-11) and salinity tolerance gene (GmSALT3) included. A total of 1489 introduced soybean accessions were genotyped by these markers with high-throughput assay as well as a SCAR marker (SCN11) which is related to soybean mosaic virus resistance. The results showed that there were 1084 accessions detected with favorable alleles; where accessions detected with resistant alleles at three loci were as much as 19, including three pyramiding SCN genes (rhg1, Rhg4, SCN3-11) which were Peking type and seven pyramiding SCN and SMV , two pyramiding SCN and salinity favorable alleles, as well as seven pyramiding SCN, SMV and salinity favorable alleles; and accessions detected with four favorable alleles were as much as nine accessions, including six pyramiding SCN and SMV resistance alleles, one accession detected with SCN and salinity tolerance and two detected with SCN, SMV and salinity favorable alleles, eight detected with all the favorable alleles in this study. Among the elite accessions mentioned above, it has been proved that 44 accessions resistant to SCN, SMV-3 or tolerant to salinity. Among the 36 accessions with three or more favorable alleles, 52.78% had been reported of one or two characteristics. Among the accessions without resistance or tolerance alleles, it has been reported that 93 accessions were tolerant to salinity or resistant to SMV-3, where new resistance or tolerance genes could be found. Screening out the accessions with high-throughput SNP detection assays for resistance and tolerance alleles in soybean provides information for their further phenotyping, screening and breeding.

Key words: soybean germplasm, molecular markers, KASP, genotyping

Jun-Hua YE,Qi-Tai YANG,Zhang-Xiong LIU,Yong GUO,Ying-Hui LI,Rong-Xia GUAN,Li-Juan QIU. Genotyping of SCN, SMV Resistance, Salinity Tolerance and Screening of Pyramiding Favorable Alleles in Introduced Soybean Accessions[J].Acta Agronomica Sinica, 2018, 44(9): 1263-1273.

Figures/Tables 6

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

携带抗性位点数 Number of resistant loci		统一编号 Accession No.	名称 Name		优异等位基因 Favorable alleles		抗性表型 Resistance
3个位点 Three loci		WDD00828	Centennial		rhg1, Rhg4, SCN3-11		SCN^*
		WDD01994	M87-1569		rhg1, Rhg4, SCN3-11		SCN^*
		WDD00691	AGS272⁺		rhg1, Rhg4, SCN3-11		SCN^*
		WDD03200	LINE272H Xuan^#		Rhg4, SCN3-11, SALT3(H1)		SCN, Salt
		WDD00289	T221		rhg1, Rhg4, SALT3(H1)		SCN^*, Salt
		WDD00858	N80-2317^#		rhg1, SCN3-11, SCN11		SCN, SMV
		WDD00859	N80-50232^#		rhg1, SCN3-11, SCN11		SCN, SMV
		WDD00666	AGS65⁺		rhg1, SCN3-11, SCN11		SCN, SMV
携带抗性位点数 Number of resistant loci	统一编号 Accession No.			名称 Name		优异等位基因 Favorable alleles		抗性表型 Resistance
	WDD00683			AGS175⁺		rhg1, SCN3-11, SCN11		SCN, SMV
	WDD02019			9234		Rhg4, SCN3-11, SCN11		SCN^*, SMV
	WDD01607			Delsoy4900		rhg1, Rhg4, SCN11		SCN^*, SMV
	WDD03086			G04-Ben229lR-M		rhg1, Rhg4, SCN11		SCN, SMV
	WDD02211			H6255		rhg1, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD03205			M017-1^#		rhg1, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD00916			SRE-D-14A^#		Rhg4, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD01640			Lamar		Rhg4, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD00334			Clark-G		SCN3-11, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD02252			P951341		SCN3-11, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD00335			Clark-S		SCN3-11, SALT3(H1), SCN11		SCN, Salt, SMV
4个位点 Four loci	WDD02102			PI90763		rhg1, Rhg4, SCN3-11, Salt3(H1)		SCN^*, Salt
	WDD00926			TGX814-26D^#		rhg1, Rhg4, SCN3-11, SCN11		SCN, SMV
	WDD01583			Newton		rhg1, Rhg4, SCN3-11, SCN11		SCN^*, SMV
	WDD00595			Custer		rhg1, Rhg4, SCN3-11, SCN11		SCN^*, SMV
	WDD00602			Franklin		rhg1, Rhg4, SCN3-11, SCN11		SCN^*, SMV
	WDD02015			TBD		rhg1, Rhg4, SCN3-11, SCN11		SCN, SMV
	WDD01538					rhg1, Rhg4, SCN3-11, SCN11		SCN, SMV
	WDD03083			S01-9364		rhg1, SCN3-11, SALT3(H1), SCN11		SCN^, Salt^, SMV
	WDD00774			S-10-1		Rhg4, SCN3-11, SALT3(H1), SCN11		SCN, Salt, SMV
5个位点 Five loci	WDD03084			S01-9391		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD01632			Bryan		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD01614			Rhodes		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD00721			Forrest		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD01971			D83-3349		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV
	WDD00661			A-66 Jia⁺		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN, Salt, SMV
	WDD02989			Pin-din-guan		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^, Salt^, SMV
	WDD03094			S99-2281		rhg1, Rhg4, SCN3-11, SALT3(H1), SCN11		SCN^*, Salt, SMV

References 65

[1]	Carter T E, Gizilice Z, Burton J W . Coefficient-of-parentage and genetic similarity estimates for 258 North American soybean cultivars released by public agencies during 1945-1988. Technical Bull, 1993: 1814-1982
[2]	Ude G N, Kenworthy W J, Costa J M, Cregan P B, Alvernaz J . Genetic diversity of soybean cultivars from China, Japan, North America, and North American ancestral lines determined by amplified fragment length polymorphism. Crop Sci, 2003,43:1858-1867
[3]	邱丽娟, 常汝镇, 孙建英, 李向华, 许占友, 刘立宏 . 中国大豆品种资源的评价与利用前景. 中国农业科技导报, 2000,2(5):58-61
	Qiu L J, Chang R Z, Sun J Y, Li X H, Xu Z Y, Liu L H . Prospects of evaluation and utilization of soybean germplasm in China. J Agric Sci Tech China, 2000,2(5):58-61 (in Chinese)
[4]	关荣霞, 郭娟娟, 常汝镇, 邱丽娟 . 国外种质对中国大豆育成品种遗传贡献的分子证据. 作物学报, 2007,33:1393-1398
	Guan R X, Guo J J, Chang R Z, Qiu L J . Marker-based evidence of broadening the genetic base of Chinese soybeans by using introduced soybeans. Acta Agron Sin, 2007,33:1393-1398 (in Chinese with English abstract)
[5]	刘章雄, 常汝镇, 邱丽娟 . 国家种质库保存国外大豆种质的分析研究. 植物遗传资源学报, 2009,10:68-72
	Liu Z X, Chang R Z, Qiu L J . Analysis of foreign soybean germplasm storied in the National Genebank of China. J Plant Genet Resour, 2009,10:68-72 (in Chinese with English abstract)
[6]	邱丽娟, 常汝镇, 袁翠平, 关荣霞, 刘章雄, 李英慧 . 国外大豆种质资源的基因挖掘利用现状与展望. 植物遗传资源学报, 1998,20:17-23
	Qiu L J, Chang R Z, Yuan C P, Guan R X, Liu Z X, Li Y H . Prospect and present status of gene discovery and utilization for introduced soybean germplasm . J Plant Genet Resour, 1998,20:17-23 (in Chinese with English abstract)
[7]	Lu H, Bernardo R . Molecular marker diversity among current and historical maize inbreds. Theor Appl Genet, 2001,103:613-617 doi: 10.1007/PL00002917
[8]	孔祥超, 李红梅, 耿甜, 黄文坤, 彭德良 . 大豆种质资源对大豆孢囊线虫3号和4号生理小种的抗性鉴定. 植物保护, 2012,38(1):146-150
	Kong X C, Li H M, Geng T, Huang W K, Peng D L . Resistance evaluation of soybean varieties and germplasms to the races No.3 and No.4 of soybean cyst nematode Heterodera glycines.Plant Prot, 2012,38(1):146-150 (in Chinese with English abstract)
[9]	Lakhssassi N, Liu S, Bekal S, Zhou Z, Colantonio V, Lambert K, Barakat A, Meksem K . Characterization of the Soluble NSF Attachment Protein gene family identifies two members involved in additive resistance to a plant pathogen. Sci Rep, 2017,7:45226
[10]	Kadam S, Vuong T D, Qiu D, Meinhardt C G, Song L, Deshmukh R, Patil G, Wan J R, Valliyodan B, Scaboo A M, Shannon J G, Nguyen H T . Genomic-assisted phylogenetic analysis and marker development for next generation soybean cyst nematode resistance breeding. Plant Sci, 2016,242:342-350
[11]	史学晖, 李英慧, 于佰双, 郭勇, 王家军, 邱丽娟 . 大豆胞囊线虫主效抗病基因Rhg4 (GmSHMT)的CAPS/dCAPS标记开发和利用. 作物学报, 2015,41:1463-1471
	Shi X H, Li Y H, Yu B S, Guo Y, Wang J J, Qiu L J . Development and utilization of CAPS/dCAPS markers based on the SNPs lying in soybean cyst nematode resistant genes Rhg4. Acta Agron Sin, 2015,41:1463-1471 (in Chinese with English abstract)
[12]	Zheng C, Chang R, Qiu L, Chen P, Wu X, Chen S . Identification and characterization of a RAPD/SCAR marker linked to a resistance gene for soybean mosaic virus in soybean. Euphytica, 2003,132:199-210
[13]	关荣霞, 陈玉波, 方宏亮, 刘硕, 腾卫丽, 李文滨, 王丕武, 常汝镇, 邱丽娟 . 中品95-5117抗大豆花叶病毒基因源分析. 作物学报, 2010,36:549-554
	Guan R X, Chen Y B, Fang H L, Liu S, Teng W L, Li W B, Wang P W, Chang R Z, Qiu L J . Origin analysis of resistance gene to soybean mosaic virus in soybean line ICGR95-5117. Acta Agron Sin, 2010,36:549-554 (in Chinese with English abstract)
[14]	Guan R X, Qu Y, Guo Y, Yu L L, Liu Y, Jiang J H, Chen J G, Ren Y L, Liu G Y, Tian L, Jin L G, Liu Z X, Hong H L, Chang R Z, Gilliham M, Qiu L J . Salinity tolerance in soybean is modulated by natural variation in GmSALT3. Plant J, 2014,80:937-950 doi: 10.1111/tpj.12695 pmid: 25292417
[15]	李金璐, 王硕, 于婧, 王玲, 周世良 . 一种改良的植物DNA提取方法. 植物学报, 2013,48:72-78
	Li J L, Wang S, Yu J, Wang L, Zhou S L . A modified CTAB protocol for plant DNA extraction. Bull Bot, 2013,48:72-78 (in Chinese)
[16]	Neelam K, Guedira G B, Huang L . Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr21. Mol Breed, 2013,31:233-237
[17]	Liu S, Kandoth P K, Warren S D, Yeckel G, Heinz R, Alden J, Yang C, Jamai A, Mellouki T E, Juvale P S, Hill J, Baum T J, Cianzio S, Whitham S A, Korkin D, Mitchum M G, Meksem K . A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens. Nature, 2012,492:256-260
[18]	Cook D E, Bayless A M, Wang K, Guo X, Song Q, Jiang J, Bent A F . Distinct copy number, coding sequence, and locus methylation patterns underlie Rhg1-mediated soybean resistance to soybean cyst nematode. Plant Physiol, 2014,165:630-647
[19]	中国农业科学院作物科学研究所. 中国大豆品种资源目录(续编三). 北京: 中国农业大学出版社, 2013. pp 1-255
	Institute of Crop Sciences , Chinese Academy of Agricultural Sciences. Catalogues of Chinese Soybean Germplasm Resources: continuation III. Beijing: China Agricultural University Press, 2013. pp 1-255(in Chinese)
[20]	张文慧 . 大豆灰斑病1号生理小种抗性基因分子标记及资源分析. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2004
	Zhang W H . Analysis of Resistant Gene against Cercospora sojina Race1 in Soybean with Molecular Markers and Germplasm Identification. MS Thesis of Northeast Agricultural University, Harbin,China, 2004 ( in Chinese with English abstract)
[21]	宋健 . 大豆种皮色相关基因定位与利用研究. 哈尔滨师范大学硕士学位论文, 黑龙江哈尔滨, 2012
	Song J . Mapping and Utilization of Genes Related to Seed Coat Color in Soybean (Glycine max (L.) Merr.).MS Thesis of Harbin Normal University, Harbin,China, 2012 ( in Chinese with English abstract)
[22]	黄志平, 王维虎, 张磊, 胡晨, 于国宜, 李杰坤, 胡国玉, 吴倩, 王大刚 . 分子标记辅助黄淮大豆生育期组归属研究. 中国油料作物学报, 2016,38(6):713-721
	Wang Z P, Wang W H, Zhang L, Hu C, Yu G Y, Li J K, Hu G Y, Wu Q, Wang D G . Maturity group classification of soybean varieties with molecular marker in Huang-Huai region. Chin J Oil Crop Sci, 2016,38(6):713-721 (in Chinese with English abstract)
[23]	Guo Y, Qiu L J . Allele-specific marker development and selection efficiencies for both flavonoid 3’-hydroxylase and flavonoid 3’,5’-hydroxylase genes in soybean subgenus soja. Theor Appl Genet, 2013,126:1445-1455
[24]	Demore P D S, Uneda-Trevisoli S H, Mauro A O D, Morceli T G S, Muniz F R S, Costa M M, Sarti D G P, Mancini M C . Validation of microsatellite markers for assisted selection of soybean genotypes resistant to powdery mildew. Crop Breed Appl Biotechnol, 2009,9:45-51
[25]	Shi Z, Bachleda N, Pham A T, Bilyeu K, Shannon G, Nguyen H, Li Z . High-throughput and functional SNP detection assays for oleic and linolenic acids in soybean. Mol Breed, 2015,35:176-186 doi: 10.1007/s11032-015-0368-4
[26]	LGC Genomics . KASP genotyping chemistry user guide and manual.LGC , 2013 [ 2018-04-12].
[27]	Semagn K, Babu R, Hearne S, Olsen M . Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement. Mol Breed, 2014,33:1-14
[28]	Jatayev S, Kurishbayev A, Zotova L, Khasanova G, Serikbay D, Zhubatkanov A, Botayeva M, Zhumalin A, Turbekova A, Soole K, Langridge P, Shavrukov Y . Advantages of Amplifluor-like SNP markers over KASP in plant genotyping. BMC Plant Biol, 2017,17:254-264 doi: 10.1186/s12870-017-1197-x
[29]	Rosas J E, Bonnecarrère V, Vida F P . One-step, codominant detection of imidazolinone resistance mutations in weedy rice ( Oryza sativa L.). Electron J Biotechnol, 2014,17:95-101
[30]	Zaidi P H, Rashid Z, Vinayan M T, Almeida G D, Phagna R K, Babu R . QTL mapping of agronomic waterlogging tolerance using recombinant inbred lines derived from tropical maize ( Zea mays L.) germplasm. PLoS One, 2015,10:e0124350
[31]	Abdulmalik R O, Menkir A, Meseka S K, Unachukwu N, Ado S G, Olarewaju J D, Aba D A, Hearne S, Crossa J, Gredil M . Genetic gains in grain yield of a maize population improved through marker assisted recurrent selection under stress and non-stress conditions in West Africa. Front Plant Sci, 2017,8:841-851
[32]	Wu J H, Liu S, Wang Q, Zeng Q, Mu J, Huang S, Yu S, Han D, Kang Z . Rapid identification of an adult plant stripe rust resistance gene in hexaploid wheat by high-throughput SNP array genotyping of pooled extremes. Theor Appl Genet, 2018,131:43-48
[33]	Tan C T, Yu H, Yang Y, Xu X, Chen M, Rudd J C, Xue Q , Ibrahim A M H, Garza L, Wang S, Mark E S, Liu S . Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat. Theor Appl Genet, 2017,130:1867-1884
[34]	Yang Z J, Chen Z Y, Peng Z S, Yu Y, Liao M L, Wei S H . Development of a high-density linkage map and mapping of the three-pistil gene ( Pis1) in wheat using GBS markers. BMC Genomics, 2017,18:567-574
[35]	Chandra S, Singh D, Pathak J, Kumari S, Kumar M, Poddar R, Balyan H S, Prabhu K V, Gupta P K, Mukhopadhyay K . SNP discovery from next-generation transcriptome sequencing data and their validation using KASP assay in wheat ( Triticum aestivum L.). Mol Breed, 2017,37:92-105
[36]	Gao L, Cook J K, Bajgain P, Zhang X, Chao S, Rouse M N, Anderson J A . Development of genotyping by sequencing (GBS)- and array-derived SNP markers for stem rust resistance gene Sr42. Mol Breed, 2015,35:207-218
[37]	Khazaei H, Purves R W, Song M, Stonehouse R, Bett K E, Stoddard F L, Vandenberg A . Development and validation of a robust, breeder-friendly molecular marker for the vc - locus in faba bean. Mol Breed , 2017,37:140-145
[38]	Patil G, Chaudhary J, Vuong T D, Jenkins B, Qiu D, Kadam S, Shannon G J, Nguyen H T . Development of SNP genotyping assays for seed composition traits in soybean. Int J Plant Genomics, 2017,2017:6572969 doi: 10.1155/2017/6572969 pmid: 28630621
[39]	Patil G, Do T, Vuong T D, Valliyodan B, Lee J D, Chaudhary J, Shannon J G, Nguyen H T . Genomic-assisted haplotype analysis and the development of high-throughput SNP markers for salinity tolerance in soybean. Sci Rep, 2016,6:19199-19211
[40]	Pham A T, Harris D K, Buck J, Hoskins A, Serrano J, Haleem H A, Cregan P, Song Q, Boerma H R, Li Z . Fine mapping and characterization of candidate genes that control resistance to Cercospora sojina K. Hara in two soybean germplasm accession. PLoS One, 2015,10:e0126753
[41]	Maroof M A S, Jeong S C, Gunduz I, Tucker D M, Buss G R, Tolin S A . Pyramiding of soybean mosaic virus resistance genes by marker-assisted selection. Crop Sci, 2007,48:517-526 doi: 10.2135/cropsci2007.08.0479
[42]	Wang D G, Zhao L, Li K, Ma Y, Wang L Q, Yang Y Q, Yang Y H, Zhi H J . Marker-assisted pyramiding of soybean resistance genes R_SC4, R_SC8, and R_SC14Q to soybean mosaic virus. J Integr Agric, 2017,16:2413-2420
[43]	Kumar V A, Balachiranjeevi C H, Naik S B, Rekha G, Rambabu R, Harika G, Pranathi K, Hajira S K, Anila M, Kousik M, Kale R, Kumar T D, Prasad M S , Prasad A S H, Padmakumari A P, Laha G S, Balachandran S M, Madhav M S, Senguttuvel P, Kemparajau K B, Fiyaz A R, Bentur J S, Viraktamath B C, Babu V R, Sundaram R M . Marker-assisted pyramiding of bacterial blight and gall midge resistance genes into RPHR-1005, the restorer line of the popular rice hybrid DRRH-3. Mol Breed, 2017,37:86-101
[44]	Hur Y J, Cho J H, Park H S, Noh T H, Park D S, Lee J Y, Sohn Y B, Shin D, Song Y C, Kwon Y U, Lee J H . Pyramiding of two rice bacterial blight resistance genes, Xa3 and Xa4, and a closely linked cold-tolerance QTL on chromosome 11. Theor Appl Genet, 2016,129:1861-1871
[45]	姚姝, 陈涛, 张亚东, 朱镇, 赵庆勇, 周丽慧, 赵凌, 赵春芳, 王才林 . 利用分子标记辅助选择聚合水稻Pi-ta、Pi-b和Wx-mq基因. 作物学报, 2017,43:1622-1631
	Yao S, Chen T, Zhang Y D, Zhu Z, Zhao Q Y, Zhou L H, Zhao L, Zhao C F, Wang C L . Pyramiding Pi-ta, Pi-b, and Wx-mq genes by marker-assisted selection in rice(Oryza sativa L.). Acta Agron Sin, 2017,43:1622-1631 (in Chinese with English abstract)
[46]	Orf J H , MacDnald D H, Wallace M K . Registration of M87-1569 soybean germplasm resistant to soybean cyst nematode. Crop Sci, 1995,35:1516 doi: 10.2135/cropsci1995.0011183X003500050064x
[47]	Hartwig E E . Breeding productive soybean cultivars resistant to the soybean cyst nematode for the Southern United States. Plant Dis, 1981,65:303-307
[48]	Dropkin V H . Soybean cyst nematode control. Plant Dis, 1984,68:829-833
[49]	Zhang J, Arelli P R, Sleper D A, Qiu B X, Ellersieck M R . Genetic diversity of soybean germplasm resistant to Heterodera glycines. Euphytica, 1999,107:205-216
[50]	Tabor G M, Tylka G L, Behm J E, Bronson C . Heterodera glycines infection increases incidence and severity of brown stem rot in both resistant and susceptible soybean . Plant Dis, 2003,87:655-661
[51]	姜奇彦, 胡正, 张辉, 王萌萌, 唐俊源, 倪志勇, 姜锋 . 大豆种质资源耐盐性鉴定与研究. 植物遗传资源学报, 2012,13:726-732
	Jiang Q Y, Hu Z, Zhang H, Wang M M, Tang J Y, Ni Z Y, Jiang F . Evaluation for salt tolerance in soybean cultivars ( Glycine max L. Merrill). J Plant Genet Resour, 2012,13:726-732 (in Chinese with English abstract)
[52]	郑翠明, 常汝镇, 邱丽娟, 吴宗璞, 高凤兰 . 大豆种质资源对SMV3号株系的抗性鉴定. 大豆科学, 2000,19:299-306
	Zheng C M, Chang R Z, Qiu L J, Wu Z P, Gao F L . Identification the resistance of soybean germplasm to SMV3. Soybean Sci, 2000,19:299-306 (in Chinese)
[53]	Klepadlo M . Genetic Analysis of Soybean Mosaic Virus (SMV) Resistance Genes in Soybean (Glycine max L. Merr). PhD Dissertation of University of Arkansas, Fayetteville, USA, 2016
[54]	Chen P Y, Ma G, Buss G R, Gunduz I, Roane C W, Tolin S A . Inheritance and allelism tests of raiden soybean for resistance to soybean mosaic virus. J Hered, 2001,92:51-55 doi: 10.1093/jhered/92.1.51 pmid: 11336229
[55]	张淋淋 . 大豆花叶病毒东北3号株系全基因组序列分析及感染性克隆的构建. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2016
	Zhang L L . The Complete Genome Sequence Analysis and Construction of Infectious Clone of the SMV Strain 3 from the Northeastern Regions of China. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang,China, 2016 ( in Chinese with English abstract)
[56]	杨春燕 . 不同来源大豆资源农艺性状分析与比较. 河北农业大学硕士学位论文, 河北保定, 2012
	Yang C Y . Analysis and Comparison of Agronomic Traits on Soybean Germplasm from Different Sources. MS Thesis of Hebei Agricultural University Baoding, Hebei,China, 2012 ( in Chinese with English abstract)
[57]	Gardner M, Heinz R, Wang J, Mitchum M G . Genetics and adaption of soybean cyst nematode to broad spectrum soybean resistance. G3: Genes Genom Genet, 2017,7:3835-3841 doi: 10.1534/g3.116.035964
[58]	Melito S, Heuberger A L, Cook D, Diers B W , Guidwin A E M, Bent A F . A nematode demographics assay in transgenic roots reveals no significant impacts of the Rhg1 locus LRR-Kinase on soybean cyst nematode resistance. BMC Plant Biol, 2010,10:104-117
[59]	Liu S, Kandoth P K, Lakhssassi N, Kang J, Colantonio V, Heinz R, Yeckel G, Zhou Z, Beckal S, Dapprich J, Rotter B, Cianzio S, Mitchum M G, Meksem K . The soybean GmSNAP18 gene underlies two types of resistance to soybean cyst nematode. Nat Commun, 2017,8:14822-14832
[60]	Noel G R, Sikora E J . Evaluation of soybeans in maturity groups I-IV for resistance to Heterodera glycines. J Nematol, 1990,22:795-799
[61]	Glover K D, Wang D, Arelli P R, Carlson S R, Cianzio S R, Diers B W . Near isogenic lines confirm a soybean cyst nematode resistance gene from PI88788 on linkage group[J]. Crop Sci, 2004,12:1252-1254
[62]	Nickell C D, Noel G R, Tharp J E, Cary T R, Thomas D J . Registration of ‘Yale’ soybean. Crop Sci, 1995,35:1221
[63]	Shi Z, Liu S, Noe J, Arelli P, Meksem K, Li Z . SNP identification and marker assay development for high-throughput selection of soybean cyst nematode resistance. BMC Genomics, 2015,16:314-325
[64]	Lübberstedt T, Melchinger A E, Fähr S, Klein D, Dally A, Westhoff P . QTL mapping in testcrosses of flint lines of maize: III. Comparison across populations for forage traits. Crop Sci, 1997,38:1278-1289
[65]	贺道华, 雷忠萍, 邢宏宜 . 功能标记的开发、特点和应用研究进展. 西北农林科技大学学报(自然科学版), 2009,37(1):110-116
	He D H, Lei Z P, Xing H Y . Development progress, characteristics and application of functional marker. J Northwest A&F Univ( Nat Sci Edn), 2009,37(1):110-116 (in Chinese with English abstract)

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性状 Trait	标记 Marker	标记类型 Type	基因 Gene	引物序列信息 Primer sequence (5°-3°)	等位基因 Allele	参考文献 Reference
大豆胞囊线虫抗性 SCN resistance
	Rhg4-389	KASP	Glyma08g11490	Rhg4-COM: CTACACCGCCGTCCTCAAC		[17]
	Rhg4-389	KASP	Glyma08g11490	Rhg4-FAM: GAGGTGGCCGCCGGAGC	G/G
				Rhg4-HEX: GAGGTGGCCGCCGGAGG	C/C
	rhg1	KASP	Glyma18g02590	rhg1-COM: CTGGCATCTGCCAACTCTGTAAAGA		[18]
			Glyma18g02590	rhg1-FAM: TTCTAATGCATTGGTTATAGCAACAACG	C/C	[18]
				rhg1-HEX: TTCTAATGCATTGGTTATAGCAACAACC	G/G
	SCN3-11	KASP	Glyma11g35820	SCN3-11-COM: CAGAAGTCGATTGAGATTTACGAAGAGATA		[9]
				SCN3-11-FAM: ATTATTGTTGAGGGATTGGCGAGC	C/C
				SCN3-11-HEX: AAATTATTGTTGAGGGATTGGCGAGT	T/T
大豆耐盐性 Soybean salinity tolerance
	SALT3	KASP	Glyma03g32900	GmSALT3-COM: GATCATTGGATGTAATTGGGTGGAGAA		[14]
			Glyma03g32900	GmSALT3-FAM: GATACCAGCAAATATTAAATGTGTGTTTTT	T/T
				GmSALT3-HEX: GATACCAGCAAATATTAAATGTGTGTTTTA	-/-
	Ncl-5	KASP	Glyma03g32900	Ncl-5-COM: AGGTACTTACCCTTATGAAGAAAACA		[14]
			Glyma03g32900	Ncl-5-FAM: AGAACTCGTATTTTATTTTGGTTGAC	G/G
				Ncl-5-HEX: AGAACTCGTATTTTATTTTGGTTGAT	A/A
大豆花叶病毒抗性 SMV resistance
	SCN11	SCAR	—	SCN11-F: TTCACGTGGCCCTCCTATC	—	[12]
				SCN11-R: CGCCGCAAACTCACAGGAC	—

性状 Trait	标记名称 Marker	种质有效鉴定数目 Accessions effectively identified No.	等位基因 Allele	对应表型¹⁾ Corresponding phenotype ¹⁾	种质Accessions
性状 Trait	标记名称 Marker	种质有效鉴定数目 Accessions effectively identified No.	等位基因 Allele	对应表型¹⁾ Corresponding phenotype ¹⁾	数量 Size (No.)	比例 Ratio (%)
大豆胞囊线虫抗性 SCN resistance	rhg1	1383	G/G	R	31	2.24
			C/C	S	1352	97.76
	Rhg4-389	1480	G/G	R	37	2.50
			C/C	S	1441	97.37
	SCN3-11	1356	T/T	R	66	4.87
			C/C	S	1290	95.13
大豆耐盐性 Soybean salinity tolerance	SALT3	1380	-/-	—	236	17.10
			T/T	S	1127	81.67
	Ncl-5	1479	G/G	—	1384	93.58
			A/A	S	85	5.75
	SALT3, Ncl-5	1380	SALT3(H1)	T	235	17.03
大豆花叶病毒抗性 SMV resistance	SCN11	1489	—	R	960	64.47
大豆花叶病毒抗性 SMV resistance			—	S	529	35.53

	抗性特性 Resistance	优异基因型 Favorable allele	携带优异等位基因的种质 Germplasm with favorable alleles
	抗性特性 Resistance	优异基因型 Favorable allele	数目 Size (No.)	比例 Ratio (%)	代表性种质 Representative germplasm
单位点 Single locus			901	60.51
	SCN	SCN3-11	15	1.01	中特1号 Zhongte 1
	SMV	SCN11	796	53.46	Provar
	Salt	SALT3(H1)	90	6.04	Harosoy 71
双位点 Two loci			147	9.87
	SCN	rhg1/Rhg4	1	0.07	M044
	SCN	Rhg4/SCN3-11	2	0.13	PR 149-3
	SCN, SMV	rhg1/SCN11	2	0.13	PI576145
	SCN, SMV	Rhg4/SCN11	5	0.34	Mack
	SCN, SMV	SCN3-11/SCN11	12	0.81	CA50
	SCN, Salt	SCN3-11/SALT3(H1)	8	0.54	PI590579
	SCN, Salt	Rhg4/SALT3(H1)	2	0.13	D85-10412
	SMV, Salt	SALT3(H1)/SCN11	115	7.72	Lee 68
3个位点 Three loci			19	1.28
	SCN	rhg1/Rhg4/SCN3-11	3	0.20	Centennial
	SCN, SMV	rhg1/Rhg4/SCN11	2	0.13	Delsoy4900
	SCN, SMV	rhg1/SCN3-11/SCN11	4	0.27	AGS175
	SCN, SMV	Rhg4/SCN3-11/SCN11	1	0.07	9234
	SCN, Salt	rhg1/Rhg4/SALT3(H1)	1	0.07	T221
	SCN, Salt	Rhg4/SCN3-11/SALT3(H1)	1	0.07	LINE272H选 LINE272H Xuan
	SCN, SMV, Salt	rhg1/SALT3(H1)/SCN11	2	0.13	H6255RR
	SCN, SMV, Salt	Rhg4/SALT3(H1)/SCN11	2	0.13	Lamar
	SCN, SMV, Salt	SCN3-11/SALT3(H1)/SCN11	3	0.20	P951341RR
4个位点 Four loci			9	0.60
	SCN, SMV	rhg1/Rhg4/SCN3-11/SCN11	6	0.40	Newton
	SCN, Salt	rhg1/Rhg4/SCN3-11/SALT3(H1)	1	0.07	PI90763
	SCN, SMV, Salt	rhg1/SCN3-11/SALT3(H1)/SCN11	1	0.07	S01-9364
	SCN, SMV, Salt	Rhg4/SCN3-11/SALT3(H1)/SCN11	1	0.07	S-10-1
5个位点 Five loci			8	0.54
	SCN, SMV, Salt	rhg1/Rhg4/SCN3-11/SALT3(H1)/SCN11	8	0.54	Pin-din-guan

Genotyping of SCN, SMV Resistance, Salinity Tolerance and Screening of Pyramiding Favorable Alleles in Introduced Soybean Accessions

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