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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1168-1175.doi: 10.3724/SP.J.1006.2016.01168


Association Analysis for MixographProperties in Ningmai 9 and Its Derivatives

JIANG Peng,ZHANG Ping-Ping,ZHANG Xu,CHEN Xiao-Lin,MA Hong-Xiang*   

  1. Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Key Laboratory for Agrobiology / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, China
  • Received:2015-11-17 Revised:2016-03-14 Online:2016-08-12 Published:2016-05-09
  • Contact: 马鸿翔, E-mail: hxma@jaas.ac.cn E-mail:hmjp2005@163.com
  • Supported by:

    Thisstudy was supported by China Agriculture Research System (CARS-3), Jiangsu Provincial Fundfor Independent Innovation in Agricultural Sciences(CX14-2002),andJiangsu Science & Technology Pillar Program(BE2013439, BE2015352).


The mixograph property is one of the rheological properties affecting the quality of end-use products in wheat. Ningmai 9 isa soft wheat cultivar planted largely in the middle and lower reaches of Yangtze River and served as a main parent of 17released wheat cultivars. For the purpose of utilizing Ningmai 9 in molecular marker-assisted selection, 185 polymorphic SSRmarkers associated with mixograph properties were tested in Ningmai 9 and its 117 derivatives. The mixed-linear model (MLM) was used in combination of phenotypic data collected in the 2009–2010 and 2010–2011 growing seasons. A total of 13 SSRs were identified to be significantly associated with mixograph properties (P<0.01), explaining 5.71–12.33% of phenotypic variations.Among the 13 markers, three, three, two, and seven were associated with mix time (MT),peak height (PH), peak width (PW), eight-minute width (8MW),respectively. MarkersXwmc11, Xbarc320, Xbarc110,and Xgwm577 for 8MW,Xwmc594 for MT,and Xgwm299 for PWwere identified over two years. Besides, Xwmc594 and Xgwm577 were associated with both (MT) and 8MW. The six markers identified over two years all negativelyaffectedmixograph parametersat Ningmai 9 background and might be used in softwheat breeding.

Key words: Wheat, Mixograph parameters, Association analysis, Molecular markers

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