小麦粒重相关基因TaCYP78A5功能标记开发及验证

doi:10.3724/SP.J.1006.2019.91016

Abstract

Abstract:

In order to develop the functional marker of wheat grain weight related gene TaCYP78A5 (Triticum aestivum Cytochrome P450 78A5) and excavate superior alleles for 1000-grain weight, functional marker CAPS-5Ap was developed according to the different SNP loci of TaCYP78A5-2A by analyzing the TaCYP78A5 promoter sequence of 30 wheat varieties and alignment evaluation. There were five SNP loci in TaCYP78A5-2A promoter regions of 30 wheat varieties, which could be divided into two kinds of genotype, TaCYP78A5-2Ap-HapIand TaCYP78A5-2Ap-HapII. The frequency of TaCYP78A5-2Ap-HapI and TaCYP78A5-2Ap-HapII in 323 modern varieties of wheat was 17.96% and 82.04% respectively, which indicating that the CAPS-5Ap can be used to identify two genotypes of TaCYP78A5-2A promoter sequence in wheat. What's more, the correlation between the genotype data and 1000-grain weight phenotype data of 323 modern varieties was analyzed, showing that CAPS-5Ap marker associated with grain weight, and TaCYP78A5-2Ap-HapII was the superior genotype for 1000-grain weight. These results may provide useful information for molecular marker assisted selection and character improvement in breeding for high yield wheat.

Key words: wheat, grain weight, TaCYP78A5-2A, SNP, functional marker

Wen-Jie SI,Lin-Nan WU,Li-Jian GUO,Meng-Die ZHOU,Xiang-Li LIU,Meng MA,Hui-Xian ZHAO. Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat (Triticum aestivum L.)[J].Acta Agronomica Sinica, 2019, 45(12): 1905-1911.

Figures/Tables 10

Table 1

Fig. 1

Supplementary Fig. 1

Table 2

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Supplementary Table 1

Haploidtype statistics of CAPS-5Ap functional marker in 323 modern varieties grown in Huang-Huai wheat region"

序号 Number	名称 Name	基因型 Haploidtype	千粒重 1000-grain weight (g)
1	大荔52	TaCYP78A5-2Ap-HapI	21.70
2	丰产1号	TaCYP78A5-2Ap-HapI	37.23
3	复壮30	TaCYP78A5-2Ap-HapI	24.63
4	衡95观26	TaCYP78A5-2Ap-HapI	36.53
5	衡观35	TaCYP78A5-2Ap-HapI	37.67
6	衡水6404	TaCYP78A5-2Ap-HapI	35.03
7	洛阳8628	TaCYP78A5-2Ap-HapI	39.80
8	石4185	TaCYP78A5-2Ap-HapI	37.27
9	石家庄8号	TaCYP78A5-2Ap-HapI	39.67
10	石麦12	TaCYP78A5-2Ap-HapI	42.60
11	石麦15	TaCYP78A5-2Ap-HapI	36.33
12	石麦18	TaCYP78A5-2Ap-HapI	38.50
13	徐州6号	TaCYP78A5-2Ap-HapI	37.80
14	豫麦48	TaCYP78A5-2Ap-HapI	42.87
15	豫麦8号	TaCYP78A5-2Ap-HapI	35.30
16	豫农949	TaCYP78A5-2Ap-HapI	40.47
17	周麦16	TaCYP78A5-2Ap-HapI	45.20
18	周麦18	TaCYP78A5-2Ap-HapI	40.73
19	石特14	TaCYP78A5-2Ap-HapI	28.53
20	西安8号	TaCYP78A5-2Ap-HapI	31.03
21	西农1018	TaCYP78A5-2Ap-HapI	40.13
22	济麦20	TaCYP78A5-2Ap-HapI	36.17
23	洛麦22	TaCYP78A5-2Ap-HapI	40.73
24	安86中17	TaCYP78A5-2Ap-HapI	25.50
25	白秃头	TaCYP78A5-2Ap-HapI	28.00
26	宝临9号	TaCYP78A5-2Ap-HapI	26.57
27	碧蚂1号	TaCYP78A5-2Ap-HapI	32.93
28	旱选10号	TaCYP78A5-2Ap-HapI	35.57
29	旱选11	TaCYP78A5-2Ap-HapI	36.20
30	旱选12	TaCYP78A5-2Ap-HapI	36.13
31	冀麦22	TaCYP78A5-2Ap-HapI	34.40
32	冀麦26	TaCYP78A5-2Ap-HapI	33.00
33	冀麦38	TaCYP78A5-2Ap-HapI	37.87
34	冀审5099	TaCYP78A5-2Ap-HapI	39.20
35	晋麦17	TaCYP78A5-2Ap-HapI	38.40
36	晋麦39	TaCYP78A5-2Ap-HapI	37.73
37	晋麦68	TaCYP78A5-2Ap-HapI	40.93
38	晋麦72	TaCYP78A5-2Ap-HapI	43.60
39	晋太182	TaCYP78A5-2Ap-HapI	37.27
40	京农80鉴107	TaCYP78A5-2Ap-HapI	39.47
41	京品11	TaCYP78A5-2Ap-HapI	39.33
42	临旱917	TaCYP78A5-2Ap-HapI	40.80
43	陇鉴294	TaCYP78A5-2Ap-HapI	39.53
44	鲁德1号	TaCYP78A5-2Ap-HapI	38.47
45	蚂蚱麦	TaCYP78A5-2Ap-HapI	53.27
46	铭贤169	TaCYP78A5-2Ap-HapI	27.20
47	农大311	TaCYP78A5-2Ap-HapI	36.07
48	农大36	TaCYP78A5-2Ap-HapI	32.30
49	庆丰1号	TaCYP78A5-2Ap-HapI	33.53
50	陕农1号	TaCYP78A5-2Ap-HapI	35.60
51	四棱红葫芦头	TaCYP78A5-2Ap-HapI	28.63
52	太原566	TaCYP78A5-2Ap-HapI	41.80
53	西峰16	TaCYP78A5-2Ap-HapI	31.27
54	小白麦	TaCYP78A5-2Ap-HapI	29.40
55	新冬22	TaCYP78A5-2Ap-HapI	37.58
56	燕大1817	TaCYP78A5-2Ap-HapI	26.67
57	中苏68	TaCYP78A5-2Ap-HapI	29.97
58	紫秆白芒先	TaCYP78A5-2Ap-HapI	30.27
59	Drysdale	TaCYP78A5-2Ap-HapII	41.87
60	SALGEMMA	TaCYP78A5-2Ap-HapII	26.30
61	百农160	TaCYP78A5-2Ap-HapII	38.60
62	博爱7023	TaCYP78A5-2Ap-HapII	40.90
63	大荔1号	TaCYP78A5-2Ap-HapII	28.87
64	泛麦8号	TaCYP78A5-2Ap-HapII	34.40
65	丰产3号	TaCYP78A5-2Ap-HapII	41.70
66	丰优5号	TaCYP78A5-2Ap-HapII	38.00
67	邯05-5092	TaCYP78A5-2Ap-HapII	34.00
68	邯6172	TaCYP78A5-2Ap-HapII	40.33
69	邯郸6050	TaCYP78A5-2Ap-HapII	41.93
70	衡216	TaCYP78A5-2Ap-HapII	34.63
71	衡4399	TaCYP78A5-2Ap-HapII	40.47
72	衡5229	TaCYP78A5-2Ap-HapII	32.47
73	衡7228	TaCYP78A5-2Ap-HapII	37.47
74	衡麦2号	TaCYP78A5-2Ap-HapII	36.17
75	衡优18	TaCYP78A5-2Ap-HapII	38.00
76	淮麦18	TaCYP78A5-2Ap-HapII	37.30
77	淮麦25	TaCYP78A5-2Ap-HapII	37.10
78	淮沭10号	TaCYP78A5-2Ap-HapII	37.67
79	兰天15	TaCYP78A5-2Ap-HapII	38.10
80	良星99	TaCYP78A5-2Ap-HapII	41.87
81	洛夫林10	TaCYP78A5-2Ap-HapII	40.53
82	洛旱11	TaCYP78A5-2Ap-HapII	46.53
83	洛旱13	TaCYP78A5-2Ap-HapII	45.47
84	洛旱2号	TaCYP78A5-2Ap-HapII	35.07
85	洛旱3号	TaCYP78A5-2Ap-HapII	39.60
86	洛旱6号	TaCYP78A5-2Ap-HapII	49.33
87	洛旱7号	TaCYP78A5-2Ap-HapII	49.73
88	洛旱8号	TaCYP78A5-2Ap-HapII	35.97
89	洛旱9号	TaCYP78A5-2Ap-HapII	51.67
90	洛麦21	TaCYP78A5-2Ap-HapII	41.33
91	洛麦23	TaCYP78A5-2Ap-HapII	36.33
92	洛农10号	TaCYP78A5-2Ap-HapII	34.53
93	漯麦8号	TaCYP78A5-2Ap-HapII	41.67
94	漯麦9号	TaCYP78A5-2Ap-HapII	40.53
95	漯优7号	TaCYP78A5-2Ap-HapII	41.73
96	青春1号	TaCYP78A5-2Ap-HapII	29.07
97	青春2号	TaCYP78A5-2Ap-HapII	26.33
98	清山843	TaCYP78A5-2Ap-HapII	35.73
99	石家庄407	TaCYP78A5-2Ap-HapII	36.20
100	石麦13	TaCYP78A5-2Ap-HapII	42.87
101	石麦19	TaCYP78A5-2Ap-HapII	35.00
102	徐州21	TaCYP78A5-2Ap-HapII	38.53
103	偃展1号	TaCYP78A5-2Ap-HapII	41.00
104	豫保1号	TaCYP78A5-2Ap-HapII	38.47
105	豫麦13	TaCYP78A5-2Ap-HapII	41.87
106	豫麦18	TaCYP78A5-2Ap-HapII	37.73
107	豫麦29	TaCYP78A5-2Ap-HapII	31.93
108	豫麦2号	TaCYP78A5-2Ap-HapII	34.57
109	豫麦38	TaCYP78A5-2Ap-HapII	41.93
110	豫麦47	TaCYP78A5-2Ap-HapII	39.07
111	豫农416	TaCYP78A5-2Ap-HapII	43.93
112	豫展4号	TaCYP78A5-2Ap-HapII	42.47
113	周麦22	TaCYP78A5-2Ap-HapII	43.40
114	周麦23	TaCYP78A5-2Ap-HapII	40.60
115	石优17	TaCYP78A5-2Ap-HapII	34.60
116	石优20	TaCYP78A5-2Ap-HapII	32.17
117	皖麦19	TaCYP78A5-2Ap-HapII	33.87
118	温麦6号	TaCYP78A5-2Ap-HapII	34.53
119	西农189	TaCYP78A5-2Ap-HapII	44.27
120	西农219	TaCYP78A5-2Ap-HapII	30.90
121	西农318	TaCYP78A5-2Ap-HapII	41.13
122	西农6028	TaCYP78A5-2Ap-HapII	29.83
123	西农688	TaCYP78A5-2Ap-HapII	46.80
124	西农797	TaCYP78A5-2Ap-HapII	45.20
125	西农9106	TaCYP78A5-2Ap-HapII	47.13
126	鑫麦296	TaCYP78A5-2Ap-HapII	37.33
127	济麦19	TaCYP78A5-2Ap-HapII	39.10
128	济麦21	TaCYP78A5-2Ap-HapII	40.10
129	济麦22	TaCYP78A5-2Ap-HapII	39.93
130	济麦4号	TaCYP78A5-2Ap-HapII	38.63
131	济南10号	TaCYP78A5-2Ap-HapII	41.73
132	济南13	TaCYP78A5-2Ap-HapII	37.27
133	济南2号	TaCYP78A5-2Ap-HapII	37.27
134	济宁3号	TaCYP78A5-2Ap-HapII	36.00
135	邯4589	TaCYP78A5-2Ap-HapII	37.80
136	衡136	TaCYP78A5-2Ap-HapII	34.90
137	济麦6号	TaCYP78A5-2Ap-HapII	35.20
138	青麦7号	TaCYP78A5-2Ap-HapII	29.33
139	西农1043	TaCYP78A5-2Ap-HapII	45.20
140	红良4号	TaCYP78A5-2Ap-HapII	37.20
141	晋麦16	TaCYP78A5-2Ap-HapII	40.07
142	晋麦25	TaCYP78A5-2Ap-HapII	35.80
143	运旱22-33	TaCYP78A5-2Ap-HapII	43.27
144	霸王鞭	TaCYP78A5-2Ap-HapII	37.67
145	白糙麦	TaCYP78A5-2Ap-HapII	26.57
146	白齐麦	TaCYP78A5-2Ap-HapII	28.53
147	宝麦5号	TaCYP78A5-2Ap-HapII	36.30
148	北京837	TaCYP78A5-2Ap-HapII	39.93
149	北京8686	TaCYP78A5-2Ap-HapII	36.43
150	北京8694	TaCYP78A5-2Ap-HapII	41.40
151	北农2号	TaCYP78A5-2Ap-HapII	35.60
152	沧麦6001	TaCYP78A5-2Ap-HapII	40.53
153	沧麦6005	TaCYP78A5-2Ap-HapII	41.27
154	沧州小麦	TaCYP78A5-2Ap-HapII	41.87
155	昌乐5号	TaCYP78A5-2Ap-HapII	40.27
156	长4640	TaCYP78A5-2Ap-HapII	44.67
157	长4738	TaCYP78A5-2Ap-HapII	41.60
158	长4853	TaCYP78A5-2Ap-HapII	41.93
159	长5259	TaCYP78A5-2Ap-HapII	34.70
160	长6154	TaCYP78A5-2Ap-HapII	42.87
161	长6359	TaCYP78A5-2Ap-HapII	33.80
162	长6452	TaCYP78A5-2Ap-HapII	44.33
163	长6794	TaCYP78A5-2Ap-HapII	35.83
164	长6878	TaCYP78A5-2Ap-HapII	36.13
165	长8744	TaCYP78A5-2Ap-HapII	36.67
166	长麦6135	TaCYP78A5-2Ap-HapII	39.40
167	长武131	TaCYP78A5-2Ap-HapII	39.47
168	长武134	TaCYP78A5-2Ap-HapII	43.53
169	长武89(1)3-4	TaCYP78A5-2Ap-HapII	42.13
170	长治516	TaCYP78A5-2Ap-HapII	33.97
171	长治620	TaCYP78A5-2Ap-HapII	58.13
172	单R8043	TaCYP78A5-2Ap-HapII	36.13
173	单R8093	TaCYP78A5-2Ap-HapII	37.80
174	单R8108	TaCYP78A5-2Ap-HapII	42.20
175	单R8194	TaCYP78A5-2Ap-HapII	38.07
176	冬协2号	TaCYP78A5-2Ap-HapII	35.27
177	丰抗13	TaCYP78A5-2Ap-HapII	34.10
178	旱选1号	TaCYP78A5-2Ap-HapII	39.87
179	旱选2号	TaCYP78A5-2Ap-HapII	34.60
180	旱选3号	TaCYP78A5-2Ap-HapII	30.07
181	黑芒麦	TaCYP78A5-2Ap-HapII	35.33
182	红和尚	TaCYP78A5-2Ap-HapII	35.67
183	葫芦头	TaCYP78A5-2Ap-HapII	38.33
184	花培6号	TaCYP78A5-2Ap-HapII	35.20
185	华北187	TaCYP78A5-2Ap-HapII	39.80
186	冀92-5203	TaCYP78A5-2Ap-HapII	32.53
187	冀麦10号	TaCYP78A5-2Ap-HapII	36.80
188	冀麦29	TaCYP78A5-2Ap-HapII	33.73
189	冀麦2号	TaCYP78A5-2Ap-HapII	38.60
190	冀麦30	TaCYP78A5-2Ap-HapII	30.00
191	冀麦32	TaCYP78A5-2Ap-HapII	39.93
192	冀麦41	TaCYP78A5-2Ap-HapII	30.80
193	冀麦6号	TaCYP78A5-2Ap-HapII	38.73
194	冀麦9号	TaCYP78A5-2Ap-HapII	40.07
195	冀麦一号	TaCYP78A5-2Ap-HapII	32.80
196	鉴26	TaCYP78A5-2Ap-HapII	31.73
197	金光	TaCYP78A5-2Ap-HapII	43.40
198	晋2148-7	TaCYP78A5-2Ap-HapII	41.20
199	晋麦13	TaCYP78A5-2Ap-HapII	35.73
200	晋麦33	TaCYP78A5-2Ap-HapII	39.47
201	晋麦44	TaCYP78A5-2Ap-HapII	38.60
202	晋麦47	TaCYP78A5-2Ap-HapII	39.67
203	晋麦50	TaCYP78A5-2Ap-HapII	38.13
204	晋麦51	TaCYP78A5-2Ap-HapII	38.40
205	晋麦53	TaCYP78A5-2Ap-HapII	40.93
206	晋麦54	TaCYP78A5-2Ap-HapII	42.80
207	晋麦57	TaCYP78A5-2Ap-HapII	42.67
208	晋麦63	TaCYP78A5-2Ap-HapII	44.27
209	晋麦79	TaCYP78A5-2Ap-HapII	36.87
210	晋麦91	TaCYP78A5-2Ap-HapII	40.80
211	晋农207	TaCYP78A5-2Ap-HapII	34.47
212	晋太102	TaCYP78A5-2Ap-HapII	37.80
213	晋太114	TaCYP78A5-2Ap-HapII	40.13
214	晋太1310	TaCYP78A5-2Ap-HapII	39.60
215	经411	TaCYP78A5-2Ap-HapII	39.60
216	京东82东307	TaCYP78A5-2Ap-HapII	32.53
217	京东83东65	TaCYP78A5-2Ap-HapII	35.07
218	京冬8号	TaCYP78A5-2Ap-HapII	37.20
219	京核8922	TaCYP78A5-2Ap-HapII	33.30
220	京花1号	TaCYP78A5-2Ap-HapII	34.40
221	京农79-15	TaCYP78A5-2Ap-HapII	43.80
222	京农84-6786	TaCYP78A5-2Ap-HapII	38.67
223	京品30	TaCYP78A5-2Ap-HapII	38.80
224	京品3号	TaCYP78A5-2Ap-HapII	40.87
225	京双16	TaCYP78A5-2Ap-HapII	37.50
226	京双2号	TaCYP78A5-2Ap-HapII	34.67
227	京选20	TaCYP78A5-2Ap-HapII	36.33
228	京选25	TaCYP78A5-2Ap-HapII	45.80
229	京延85鉴28	TaCYP78A5-2Ap-HapII	38.33
230	科农199	TaCYP78A5-2Ap-HapII	36.73
231	科遗26	TaCYP78A5-2Ap-HapII	35.60
232	科遗29	TaCYP78A5-2Ap-HapII	34.07
233	临138	TaCYP78A5-2Ap-HapII	44.47
234	临汾8050	TaCYP78A5-2Ap-HapII	37.27
235	临丰3号	TaCYP78A5-2Ap-HapII	41.80
236	临丰518	TaCYP78A5-2Ap-HapII	35.80
237	临旱5089	TaCYP78A5-2Ap-HapII	36.33
238	临旱5367	TaCYP78A5-2Ap-HapII	37.47
239	临旱6105	TaCYP78A5-2Ap-HapII	37.47
240	临旱6号	TaCYP78A5-2Ap-HapII	41.60
241	临旱935	TaCYP78A5-2Ap-HapII	42.20
242	临抗5108	TaCYP78A5-2Ap-HapII	38.03
243	陇鉴196	TaCYP78A5-2Ap-HapII	39.73
244	鲁麦14	TaCYP78A5-2Ap-HapII	41.87
245	鲁麦15	TaCYP78A5-2Ap-HapII	35.93
246	鲁麦17	TaCYP78A5-2Ap-HapII	44.60
247	鲁麦19	TaCYP78A5-2Ap-HapII	42.07
248	鲁麦23	TaCYP78A5-2Ap-HapII	44.87
249	鲁麦3号	TaCYP78A5-2Ap-HapII	38.27
250	鲁麦5号	TaCYP78A5-2Ap-HapII	35.73
251	鲁麦8号	TaCYP78A5-2Ap-HapII	38.07
252	轮抗7号	TaCYP78A5-2Ap-HapII	39.00
253	轮选987	TaCYP78A5-2Ap-HapII	35.53
254	宁冬11	TaCYP78A5-2Ap-HapII	41.40
255	农大135	TaCYP78A5-2Ap-HapII	43.20
256	农大146	TaCYP78A5-2Ap-HapII	40.27
257	农大155	TaCYP78A5-2Ap-HapII	28.97
258	农大183	TaCYP78A5-2Ap-HapII	30.13
259	农大20074	TaCYP78A5-2Ap-HapII	45.87
260	农大3159	TaCYP78A5-2Ap-HapII	46.33
261	农大33	TaCYP78A5-2Ap-HapII	37.87
262	农大81146	TaCYP78A5-2Ap-HapII	41.27
263	平凉35	TaCYP78A5-2Ap-HapII	34.20
264	平阳348	TaCYP78A5-2Ap-HapII	43.07
265	秦麦3号	TaCYP78A5-2Ap-HapII	36.07
266	秦麦7号	TaCYP78A5-2Ap-HapII	34.80
267	山农辐63	TaCYP78A5-2Ap-HapII	40.67
268	山农优麦2号	TaCYP78A5-2Ap-HapII	46.20
269	山优2号	TaCYP78A5-2Ap-HapII	43.73
270	陕225-9	TaCYP78A5-2Ap-HapII	37.33
271	陕229	TaCYP78A5-2Ap-HapII	42.80
272	陕旱8675	TaCYP78A5-2Ap-HapII	42.80
273	陕合6号	TaCYP78A5-2Ap-HapII	36.33
274	陕农2号	TaCYP78A5-2Ap-HapII	32.67
275	胜利麦	TaCYP78A5-2Ap-HapII	35.87
276	双丰收	TaCYP78A5-2Ap-HapII	38.67
277	舜麦1718	TaCYP78A5-2Ap-HapII	38.07
278	太13606	TaCYP78A5-2Ap-HapII	40.60
279	太712	TaCYP78A5-2Ap-HapII	42.53
280	太原633	TaCYP78A5-2Ap-HapII	38.33
281	泰山23	TaCYP78A5-2Ap-HapII	36.27
282	泰山24	TaCYP78A5-2Ap-HapII	39.13
283	渭麦4号	TaCYP78A5-2Ap-HapII	32.80
284	西峰20	TaCYP78A5-2Ap-HapII	31.13
285	西峰9号	TaCYP78A5-2Ap-HapII	31.70
286	小山8号	TaCYP78A5-2Ap-HapII	38.73
287	新冬20	TaCYP78A5-2Ap-HapII	40.27
288	烟农19	TaCYP78A5-2Ap-HapII	40.87
289	烟农21	TaCYP78A5-2Ap-HapII	37.33
290	延安15	TaCYP78A5-2Ap-HapII	37.87
291	原冬3号	TaCYP78A5-2Ap-HapII	40.00
292	原冬834	TaCYP78A5-2Ap-HapII	35.92
293	原冬847	TaCYP78A5-2Ap-HapII	33.33
294	原冬856	TaCYP78A5-2Ap-HapII	35.67
295	运旱102	TaCYP78A5-2Ap-HapII	38.87
296	运旱115	TaCYP78A5-2Ap-HapII	38.67
297	运旱2028	TaCYP78A5-2Ap-HapII	38.80
298	运旱20410	TaCYP78A5-2Ap-HapII	40.83
299	运旱21-30	TaCYP78A5-2Ap-HapII	40.13
300	运旱23-35	TaCYP78A5-2Ap-HapII	43.67
301	运旱618	TaCYP78A5-2Ap-HapII	39.60
302	运旱719	TaCYP78A5-2Ap-HapII	35.93
303	运旱805	TaCYP78A5-2Ap-HapII	38.87
304	早穗21	TaCYP78A5-2Ap-HapII	32.53
305	早穗65	TaCYP78A5-2Ap-HapII	32.40
306	早穗66	TaCYP78A5-2Ap-HapII	33.93
307	早洋麦	TaCYP78A5-2Ap-HapII	35.67
308	张冬29	TaCYP78A5-2Ap-HapII	33.00
309	郑丰9962	TaCYP78A5-2Ap-HapII	34.53
310	郑州24	TaCYP78A5-2Ap-HapII	36.42
311	中7902	TaCYP78A5-2Ap-HapII	44.60
312	中86I-50455	TaCYP78A5-2Ap-HapII	38.13
313	中大86-鉴2	TaCYP78A5-2Ap-HapII	34.87
314	中大91-品9	TaCYP78A5-2Ap-HapII	43.67
315	中大92-鉴49	TaCYP78A5-2Ap-HapII	39.53
316	中大92-品8	TaCYP78A5-2Ap-HapII	40.80
317	中旱110	TaCYP78A5-2Ap-HapII	43.47
318	中麦175	TaCYP78A5-2Ap-HapII	42.53
319	中麦9号	TaCYP78A5-2Ap-HapII	45.27
320	中引6号	TaCYP78A5-2Ap-HapII	37.07
321	中优9507	TaCYP78A5-2Ap-HapII	46.27
322	中作60064	TaCYP78A5-2Ap-HapII	36.20
323	中作60115	TaCYP78A5-2Ap-HapII	42.73

Supplementary Table 1

Fig. 6

References 22

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引物名称 Primer name	正向引物序列 Forward primer sequence (5′-3′)	反向引物序列 Reverse primer sequence (5′-3′)
A5-Ap	AGCCCCTTCATCTGTCGGGTAACCC	TTAGCCGGGAGGAGGAGCAGGAG
A5-Bp	GAAAAGGCGAACCACGGATCATG	TAGCCGGGGGAGGAGCAGGAG
A5-Dp	CTTGGCGAAGCCCTGCCGAGATC	CGCCCGTGAGAGCACAGGAGT
GADPH	CCTTCCGTGTTCCCACTGTTG	ATGCCCTTGAGGTTTCCCTC
A5-A-RT	CCATTCCTCAAGTGGCTCGAT	TGGGTGCACCACCATCCTC
A5-B-RT	TCATTCCTCAAGTGGCTCGAC	GGGTGCAGCACCATCCTG
A5-D-RT	TCCTGCTCGCCGTGCTC	TTGCGCGGTGGAAGAGG
CAPS-A5-Ap	TACAAAGCCGGAGCGCCTTCGCGTTCTTC	GAAGCTGGCTCCTCCCGGCCTGCG

顺式作用元件 cis-acting regulatory element	生物学功能 Biological function	出现次数Frequency
顺式作用元件 cis-acting regulatory element	生物学功能 Biological function	TaCYP78A5-2Ap	TaCYP78A5-2Bp	TaCYP78A5-2Dp
ABRE	脱落酸应答元件 cis-acting element involved in the abscisic acid responsiveness	3	5	5
ARE	厌氧感应调控元件 cis-acting regulatory element essential for the anaerobic induction	1	1	3
AT-rich sequence	诱导终止激活因子 Element for maximal elicitor-mediated activation (2copies)	1	0	0
CAAT-box	启动子和增强子常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions	10	5	5
CAT-box	分生组织表达的常见顺式作用元件 cis-acting regulatory element related to meristem expression	1	0	0
CCAAT-box	MYBHv1转录因子结合位点 MYBHv1 binding site	1	3	1
CGTCA-motif	茉莉酸甲酯响应的调控元件 cis-acting regulatory element involved in the MeJA-responsiveness	2	4	0
GC-motif	特定激素诱导增强元件 Enhancer-like element involved in anoxic specific inducibility	1	2	0
GCN4_motif	胚乳表达相关顺式作用元件 cis-regulatory element involved in endosperm expression	0	0	1
P-box	赤霉素响应元件 Gibberellin-responsive element	0	2	1
TATA-box	-30区编码核心启动子元件 Core promoter element around -30 of transcription start	11	14	6
TCA-element	水杨酸响应顺式作用元件 cis-acting element involved in salicylic acid responsiveness	0	0	1
TGA-element	激素响应元件 Auxin-responsive element	1	0	1
TGACG-motif	茉莉酸甲酯响应的调控元件 cis-acting regulatory element involved in the MeJA-responsiveness	1	4	0

Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat (Triticum aestivum L.)

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