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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (5): 792-797.doi: 10.3724/SP.J.1006.2019.84104

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Repetitive intense flashes inhibit photosystem II activity and thermal dissipation in cotton leaves

Han-Yu WU1,3,Fei XIAO1,Ya-Li ZHANG2,Chuang-Dao JIANG3,*(),Wang-Feng ZHANG2,*()   

  1. 1 College of Life Science, Shihezi University, Shihezi 832003, Xinjiang, China
    2 College of Agriculture, Shihezi University / Key Laboratory of Oasis Ecology Agriculture of Xinjiang Production and Construction Corps, Shihezi 832003, Xinjiang, China
    3 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2018-07-28 Accepted:2018-12-24 Online:2019-05-12 Published:2019-02-15
  • Contact: Chuang-Dao JIANG,Wang-Feng ZHANG E-mail:jcdao@ibcas.ac.cn;wfzhang65@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(U1803234)

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Abstract:

Not only continuous high light results in the decrease of photosynthetic efficiency, but also intense flashes may affect the photosynthetic function. In this study, cotton (Gossypium hirsutum L.) cultivar Xinluzao 45 was used to investigate the effects of repetitive intense flash treatment (leaves exposed to 20,000 μmol m -2 s -1 for 300 ms, with interval time of 10 s, and the whole treatment duration was 30 min) on two photosystems and photosynthetic function of cotton leaves. Chlorophyll fluorescence, P700 and gas exchange were measured before and after repetitive intense flash treatment, respectively. The content of active PSI (photosystem I) reaction center and the electron transfer activity of PSII (photosystem II) all decreased after repetitive intense flash treatment which reflected by the significant increase in J and K phases of the fluorescence induction kinetics curves after repetitive intense flash treatment. ΦND (the quantum yield of non-photochemical energy dissipation due to donor side limitation) of PSI decreased while ΦNA (the quantum yield of non-photochemical energy dissipation due to acceptor side limitation) increased, indicating that acceptor side of PSI was primarily inhibited by repetitive intense flashes. Repetitive intense flash treatment induced a distinct decrease in the quantum yield of PSII in cotton leaves under actinic light. Moreover, ΦNPQ (the quantum yield of regulated energy dissipation) of PSII decreased significantly after repetitive intense flash treatment. However, ΦNO (the quantum yield of non-regulated energy dissipation) increased considerably, demonstrating that the repetitive intense flashes caused PSII photoinhibition. The photosynthetic rate and the stomatal conductance decreased while the intercellular CO2 concentration increased after repetitive intense flash treatment, indicating that the reduction of carbon assimilation induced by repetitive intense flash treatment is not limited by stomata. Therefore, we believe that the repetitive intense flash treatment not only induces inactivation of PSI, but also leads to PSII photoinhibition and the decrease of thermal dissipation. The suppression of photosynthetic electron transport activity may play important role in the decrease of photosynthetic rate after repetitive intense flash treatment.

Key words: cotton, repetitive intense flashs, photoinhibition, photosynthesis, electron transport

Fig. 1

Effects of repetitive intense flashes on P700 redox state (A) and P700 maximum oxidation state (Pm) (B) in cotton leaves"

Fig. 2

Effects of repetitive intense flashes on chlorophyll a fluorescence transient of PSII (A), the maximum PSII photochemical efficiency Fv/Fm (B), VJ (C), and WK (D) in cotton leaves"

Fig. 3

Effects of repetitive intense flashes on the redox state of P700 in cotton leaves A: effective photochemical quantum yield of PSI (ΦPSI); B: fraction of overall P700 that is oxidized in a given state (ΦND), i.e. the quantum yield of non-photochemical energy dissipation due to donor side limitation; C: fraction of overall P700 that cannot be oxidized in a given state due to reduced PSI electron acceptors (ΦNA), i.e. the quantum yield of non-photochemical energy dissipation due to acceptor side limitation."

Fig. 4

Effects of repetitive intense flashes on chlorophyll a fluorescence quenching in cotton leaves A: the actual photochemical efficiency of PSII (ΦPSII); B: non-photochemical quenching (NPQ); C: the quantum yield of regulated energy dissipation (ΦNPQ); D: the quantum yield of non-regulated energy dissipation (ΦNO)."

Fig. 5

Effects of repetitive intense flash treatment on light response curves of photosynthesis in cotton leaves A: photosynthetic rate (Pn); B: stomatal conductance(Gs); C: intercellular CO2 concentration (Ci)."

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