甘蓝型油菜种子萌发期耐铝毒特性综合评价及其种质筛选

doi:10.3724/SP.J.1006.2019.84169

Abstract

Abstract:

Seed germination is an important stage of plant morphogenesis in rapeseed. Aluminum toxicity is one of the main factors affecting seed germination in acidic soil. Therefore, it is important for rapeseed production and research to screen Al-tolerant germplasms during seed germination. In this study, five Brassica napus cultivars (lines) were used to screen the optimum treatment concentration of Al toxicity at germination stage, which was determined at 90 μg mL ^-1. The germination vigor, germination rate, fresh weight, dry weight, root length and bud length of 148 cultivars (lines) of Brassica napus were measured at germination stage. The comprehensive evaluation value (A value), average subordinate function value (ASF value), weighted tolerance coefficient (WAC value), correlation coefficient, frequency, principal component, grey relational degree, clustering and stepwise regression were used to identify the Al tolerance at germination stage. A comprehensive evaluation model of Al tolerance at germination stage was established, suitable evaluation and identification indexes were selected. There were significant differences in the germination parameters of 148 Brassica napus germplasm, and aluminum-tolerant Brassica napus varieties (lines) during germination stage were screened, including 01188, WH-20, A109, and Jiayu 31 peng. The results of grey correlation and regression analysis indicated that the root length, bud length, fresh weight, germination rate and germination potential measured at the germination stage of rapeseed, and the A value of the indices estimated by regression equation, could be preliminarily judged the aluminum tolerance of rape germplasm.

Key words: Brassica napus L, germination stage, aluminum toxicity stress, screening for germplasm resources, comprehensive evaluation

GAO Huan-Huan,YE Sang,WANG Qian,WANG Liu-Yan,WANG Rui-Li,CHEN Liu-Yi,TANG Zhang-Lin,LI Jia-Na,ZHOU Qing-Yuan,CUI Cui. Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus[J].Acta Agronomica Sinica, 2019, 45(9): 1416-1430.

Figures/Tables 10

Table 1

Table 2

Fig. 1

Table 3

Table 4

Table 5

Table 6

ASF value, A value, and WAC value sequence of rapeseed variety resources under aluminum toxicity stress"

品种(系) Cultivar (line)	材料来源 Source	ASF值 ASF value	排序 Order	A值 A value	排序 Order	WAC值 WAC value	排序 Order
SWU7	重庆 Chongqing	0.35	59	0.12	58	0.90	88
SWU36	重庆 Chongqing	0.27	125	-0.61	129	0.79	136
SWU49	重庆 Chongqing	0.34	70	-0.07	80	0.90	91
SWU64	重庆 Chongqing	0.45	11	0.93	12	1.11	11
SWU69	重庆 Chongqing	0.40	25	0.44	26	1.00	33
SWU71	重庆 Chongqing	0.38	35	0.44	25	0.99	40
SWU77	重庆 Chongqing	0.40	26	0.20	45	0.97	49
SWU80	重庆 Chongqing	0.39	28	0.43	28	1.01	29
SWU81	重庆 Chongqing	0.38	39	0.11	60	1.02	27
SWU84	重庆 Chongqing	0.28	114	-0.40	114	0.86	117
SWU88	重庆 Chongqing	0.38	34	-0.07	79	0.97	45
SWU95	重庆 Chongqing	0.26	127	-0.75	136	0.85	119
SWU96	重庆 Chongqing	0.40	22	0.57	19	1.07	15
SWU99	重庆 Chongqing	0.39	31	0.17	49	0.97	43
SWU100	重庆 Chongqing	0.28	117	-0.34	109	0.86	112
SWU110	重庆 Chongqing	0.48	8	1.28	5	1.23	6
SWU111	重庆 Chongqing	0.31	98	-0.30	103	0.87	108
SWU112	重庆 Chongqing	0.37	44	0.10	62	0.98	41
1L187	湖北 Hubei	0.50	6	1.23	6	1.19	8
中双6号Zhongshuang 6	湖北 Hubei	0.41	19	0.83	14	1.07	16
1L191	湖北 Hubei	0.45	10	1.03	10	1.15	9
辐油4号 Fuyou 4	湖北 Hubei	0.32	88	-0.14	89	0.93	71
1L410	湖北 Hubei	0.29	110	-0.12	85	0.88	106
扬J6711 Yang J6711	湖北 Hubei	0.35	62	0.05	67	0.99	36
Monty	湖北 Hubei	0.35	54	0.14	54	0.97	46
史力丰 Shilifeng	湖北 Hubei	0.32	90	-0.15	90	0.91	82
青662A Qing 662A	湖北 Hubei	0.31	97	-0.30	104	0.90	89
WH-19	湖北 Hubei	0.50	7	1.15	8	1.20	7
11-9-704	湖北 Hubei	0.63	2	1.88	2	1.43	1
WH-95	湖北 Hubei	0.34	65	-0.34	108	0.91	83
NY7	湖北 Hubei	0.30	108	-0.15	91	0.88	100
华油6号 Huayou 6	湖北 Hubei	0.29	112	-0.39	113	0.84	122
华油10 Huayou 10	湖北 Hubei	0.39	29	0.32	34	0.96	51
Suigenshu	湖北 Hubei	0.43	14	0.68	18	1.03	24
华油4号 Huayou 4	湖北 Hubei	0.36	53	0.19	47	0.93	68
Y2	江苏 Jiangsu	0.30	106	-0.29	101	0.88	103
勺叶青 Shaoyeqing	江苏 Jiangsu	0.28	121	-0.46	118	0.83	125
沪激早 Lujizao	江苏 Jiangsu	0.32	87	0.14	55	0.94	64
大花球 Dahuaqiu	江苏 Jiangsu	0.33	82	-0.07	81	0.89	95
荣选 Rongxuan	江苏 Jiangsu	0.32	94	-0.21	96	0.90	87
宁油6号 Ningyou 6	江苏 Jiangsu	0.30	99	-0.45	117	0.85	118
广德8104 Guangde 8104	江苏 Jiangsu	0.38	36	0.39	30	1.01	30
宿84-6 Su 84-6	江苏 Jiangsu	0.35	60	0.06	65	0.96	50
芥65-1 Jie 65-1	江苏 Jiangsu	0.34	71	0.14	56	0.93	70
品种(系) Cultivar (line)	材料来源 Source	ASF值 ASF value	排序 Order	A值 A value	排序 Order	WAC值 WAC value	排序 Order
SWU20	重庆 Chongqing	0.29	111	-0.36	111	0.88	102
SWU28	重庆 Chongqing	0.38	37	0.29	37	0.97	44
SWU40	重庆 Chongqing	0.41	20	0.44	24	1.06	17
SWU47	重庆 Chongqing	0.36	51	0.35	31	0.87	111
SWU48	重庆 Chongqing	0.23	137	-0.74	135	0.78	137
SWU56	重庆 Chongqing	0.23	138	-0.77	137	0.80	131
SWU59	重庆 Chongqing	0.20	143	-0.80	139	0.76	141
SWU65	重庆 Chongqing	0.24	135	-0.71	133	0.80	133
SWU92	重庆 Chongqing	0.28	119	-0.40	115	0.88	105
SWU101	重庆 Chongqing	0.27	126	-0.58	127	0.85	120
川油20 Chuanyou 20	四川 Sichuan	0.38	38	0.26	41	1.04	23
川油18 Chuanyou 18	四川 Sichuan	0.28	116	-0.34	107	0.90	86
CY12Q95406	四川 Sichuan	0.36	47	0.21	43	0.93	69
CY12Q8-7	四川 Sichuan	0.30	104	-0.53	121	0.86	113
CY12QSZ06	四川 Sichuan	0.19	146	-0.99	142	0.76	142
CY12Q95108	四川 Sichuan	0.25	133	-0.58	126	0.82	127
CY12Q21535-N3	四川 Sichuan	0.35	63	0.18	48	0.96	52
wx1025	湖南 Hunan	0.23	139	-0.96	141	0.77	140
wx10315	湖南 Hunan	0.34	69	-0.27	99	0.87	110
10-1358	湖南 Hunan	0.32	85	-0.78	138	0.77	138
湘油13号 Xiangyou 13	湖南 Hunan	0.11	148	-1.54	148	0.57	148
782	湖南 Hunan	0.27	124	-0.57	125	0.80	132
WX10329	湖南 Hunan	0.23	141	-1.06	144	0.76	143
509	湖南 Hunan	0.26	130	-0.66	131	0.80	130
1321	湖南 Hunan	0.30	100	-0.12	86	0.99	39
07022	湖北 Hubei	0.44	12	-0.20	95	1.01	31
07094	湖北 Hubei	0.32	89	-0.05	76	0.89	96
97097	湖北 Hubei	0.39	30	-0.25	98	0.95	57
RQ011	湖北 Hubei	0.40	23	-0.19	94	1.01	28
96021	湖北 Hubei	0.18	147	-1.03	143	0.73	145
01111	湖北 Hubei	0.38	33	-0.05	77	0.99	37
01570	湖北 Hubei	0.44	13	0.74	16	1.04	21
01188	湖北 Hubei	0.64	1	2.02	1	1.39	2
02354	湖北 Hubei	0.34	73	0.00	74	0.92	78
02359	湖北 Hubei	0.33	79	0.30	36	0.88	99
93205	湖北 Hubei	0.27	123	-0.56	124	0.77	139
93210	湖北 Hubei	0.34	68	0.15	53	0.97	48
Nca	湖北 Hubei	0.43	16	0.87	13	1.10	12
中双4号 Zhongshuang 4	湖北 Hubei	0.34	67	0.21	44	0.95	54
中双11号 Zhongshuang 11	湖北 Hubei	0.28	120	-0.51	120	0.83	126
2011-6308	湖北 Hubei	0.37	43	0.32	33	0.95	55
2012-3448	湖北 Hubei	0.23	140	-0.95	140	0.80	129
2012-5086	湖北 Hubei	0.32	86	-0.23	97	0.94	62
2012-8380	湖北 Hubei	0.30	102	-0.18	93	0.89	94
2012-8998	湖北 Hubei	0.40	21	0.51	23	1.00	34
品种(系) Cultivar (line)	材料来源 Source	ASF值 ASF value	排序 Order	A值 A value	排序 Order	WAC值 WAC value	排序 Order
2012-9478	湖北 Hubei	0.36	48	0.25	42	1.04	20
希望106 Xiwang 106	湖北 Hubei	0.36	49	0.31	35	0.94	65
阳光198 Yangguang 198	湖北 Hubei	0.20	144	-1.19	147	0.71	147
中双10号 Zhongshuang 10	湖北 Hubei	0.35	56	0.16	52	0.92	72
中双12号 Zhongshuang 12	湖北 Hubei	0.36	50	0.44	27	0.99	35
中油589 Zhongyou 589	湖北 Hubei	0.40	24	0.53	21	1.03	25
华油13号 Huayou 13	湖北 Hubei	0.34	74	0.10	61	0.95	56
Rucabo	湖北 Hubei	0.35	55	0.05	68	0.94	66
WH-20	湖北 Hubei	0.37	42	0.26	40	1.04	22
WH-23	湖北 Hubei	0.37	41	0.27	39	1.00	32
WH-24	湖北 Hubei	0.34	77	0.12	59	0.92	76
WH-25	湖北 Hubei	0.33	78	0.00	72	0.94	60
WH-33	湖北 Hubei	0.43	15	0.95	11	1.09	13
WH-37	湖北 Hubei	0.34	66	0.06	66	0.88	101
WH-38	湖北 Hubei	0.30	105	-0.38	112	0.91	79
WH-41	湖北 Hubei	0.25	134	-0.56	123	0.79	135
WH-45	湖北 Hubei	0.31	96	-0.18	92	0.86	116
WH-49	湖北 Hubei	0.28	113	-0.46	119	0.89	93
WH-55	湖北 Hubei	0.51	5	1.13	9	1.23	5
WH-60	湖北 Hubei	0.40	27	0.41	29	1.02	26
甲预17棚 Jiayu 17 peng	湖北 Hubei	0.20	142	-1.15	146	0.72	146
甲预25棚 Jiayu 25 peng	湖北 Hubei	0.30	101	-0.12	87	0.89	92
甲预31棚 Jiayu 31 peng	湖北 Hubei	0.51	3	1.62	4	1.35	3
甲915 Jia 915	湖北 Hubei	0.42	18	0.72	17	1.08	14
甲922 Jia 922	湖北 Hubei	0.20	145	-1.08	145	0.75	144
甲917 Jia 917	湖北 Hubei	0.28	122	-0.36	110	0.88	107
甲931 Jia 931	湖北 Hubei	0.26	131	-0.61	128	0.84	123
甲963棚 Jia 963 peng	湖北 Hubei	0.34	64	0.28	38	0.97	47
沪油17号 Huyou 17	江苏 Jiangsu	0.29	109	-0.10	83	0.95	58
沪油15号Huyou 15	江苏 Jiangsu	0.46	9	1.15	7	1.13	10
宁油14号 Ningyou 14	江苏 Jiangsu	0.33	80	0.00	71	0.92	73
扬油6号 Yangyou 6	江苏 Jiangsu	0.34	72	0.09	63	0.92	77
苏油1号 Suyou 1	江苏 Jiangsu	0.30	107	-0.31	105	0.87	109
浙双8号 Zheshuang 8	江苏 Jiangsu	0.32	91	-0.03	75	0.88	104
浙油758 Zheyou 758	江苏 Jiangsu	0.28	118	-0.42	116	0.84	121
沪油19 Huyou 19	江苏 Jiangsu	0.24	136	-0.55	122	0.79	134
皖油 29 Wanyou 29	江苏 Jiangsu	0.37	40	0.52	22	0.99	38
至尊 Zhizun	青海 Qinghai	0.38	32	0.54	20	0.94	63
A117	陕西 Shaanxi	0.25	132	-0.72	134	0.89	97
B250	陕西 Shaanxi	0.34	76	-0.13	88	0.92	75
B265	陕西 Shaanxi	0.37	45	0.33	32	0.98	42
A109	陕西 Shaanxi	0.51	4	1.66	3	1.32	4
GY284	陕西 Shaanxi	0.30	103	-0.34	106	0.91	80
A97	陕西 Shaanxi	0.33	83	0.00	73	0.94	67
品种(系) Cultivar (line)	材料来源 Source	ASF值 ASF value	排序 Order	A值 A value	排序 Order	WAC值 WAC value	排序 Order
A148	陕西 Shaanxi	0.36	46	0.17	50	1.04	19
09-P32	湖北 Hubei	0.28	115	-0.29	102	0.86	114
10-P10	湖北 Hubei	0.36	52	0.13	57	0.92	74
11-P30	湖北 Hubei	0.33	81	-0.05	78	0.86	115
03IIB	甘肃 Gansu	0.34	75	0.04	69	0.91	81
DDI	甘肃 Gansu	0.33	84	0.08	64	0.91	84
06T9F	甘肃 Gansu	0.35	61	-0.07	82	0.94	61
03II4B	甘肃 Gansu	0.26	129	-0.62	130	0.84	124
03LF1	甘肃 Gansu	0.26	128	-0.70	132	0.81	128
9852	甘肃 Gansu	0.31	95	-0.28	100	0.91	85
06H7	甘肃 Gansu	0.35	57	0.20	46	0.95	59
CY18PXW-62	四川 Sichuan	0.43	17	0.77	15	1.05	18
96063	湖北 Hubei	0.35	58	0.16	51	0.90	90
浙油21 Zheyou 21	湖北 Hubei	0.32	92	0.02	70	0.96	53
97177	江苏 Jiangsu	0.32	93	-0.11	84	0.88	98

Table 6

Table 7

Fig. 2

Table 8

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浓度Concentration (μg mL^-1)	品种(系) Cultivar (line)					均值Mean	较对照变化Comparison with the control (%)
浓度Concentration (μg mL^-1)	ZS11	D011	D363	D016	D064	均值Mean	较对照变化Comparison with the control (%)
0	9.39±0.131 a	7.97±0.345 a	9.17±0.390 a	5.39±0.401 a	9.24±0.259 a	8.23	0
30	8.35±0.372 ab	8.17±0.237 a	9.04±0.068 a	5.17±0.947 a	7.66±0.119 a	7.68	-6.72
60	6.78±1.084 bc	7.34±0.377 a	5.90±0.700 b	5.34±0.541 a	7.48±0.422 a	6.57	-20.24
90	6.97±0.240 bc	6.96±0.489 a	6.10±0.493 b	4.35±0.127 a	7.95±0.631 a	6.47	-21.45
120	5.71±0.699 c	4.65±0.560 b	3.81±0.468 c	2.79±0.244 b	4.00±0.952 b	4.19	-49.07
150	3.47±0.623 d	1.61±0.263 c	3.82±0.223 c	1.68±0.287 b	4.10±0.122 b	2.50	-69.65
240	2.10±0.123 d	2.39±0.347 c	3.83±0.745 c	1.79±0.546 b	2.32±0.149 b	2.27	-72.41

	参数 Parameter	根长 RL (cm)	芽长 SL (cm)	发芽势 GV (%)	发芽率 GR (%)	鲜重 FW (g)	干重 DW (g)
对照	最大值Max.	12.75	3.09	100.00	100.00	0.55	0.06
Control	最小值Min.	1.79	0.80	12.50	40.00	0.14	0.02
	均值Average	6.72^**	1.65^**	64.88^**	69.05^**	0.33^**	0.04^**
	标准差SE	1.89	0.41	19.80	17.12	0.08	0.01
	变异系数CV (%)	28.08	24.98	30.52	24.79	24.90	21.60
处理	最大值Max.	8.68	3.29	100.00	100.00	0.47	0.09
Treatment	最小值Min.	0.49	0.86	7.50	15.00	0.12	0.02
	均值Average	4.55^**	1.73^**	68.65^**	72.18^**	0.28^**	0.03^**
	标准差SE	1.50	0.42	20.17	18.66	0.08	0.01
	变异系数CV (%)	32.96	24.49	29.38	25.85	27.71	26.41
较对照变化	均值Average (%)	-32.36	4.86	5.81	4.53	-15.60	-4.26
Comparison with the control	变异系数CV (%)	17.38	-1.96	-3.73	4.26	11.28	22.24

参数 Parameter	发芽势 GV (%)	发芽率 GR (%)	鲜重 FW (g)	干重 DW (g)	根长 RL (cm)	芽长 SL (cm)
最大值Max.	3.125	2.250	1.721	1.613	1.874	1.486
最小值Min.	0.278	0.316	0.394	0.710	0.271	0.698
均值Average	1.093 ^**	1.064 ^**	0.857^**	0.959 ^**	0.705 ^**	1.059 ^**
标准差SE	0.325	0.258	0.191	0.121	0.257	0.137
变异系数CV (%)	29.717	24.263	22.249	12.650	36.528	12.888

测定指标 Index	发芽势 GV (%)	发芽率 GR (%)	鲜重 FW (g)	干重 DW (g)	根长 RL (cm)	芽长 SL (cm)
发芽势 GV	1
发芽率 GR	0.888^**	1
鲜重 FW	0.026	0.008	1
干重 DW	-0.167	-0.159	0.407^**	1
根长 RL	0.157^*	0.227^**	0.420^**	0.116	1
芽长 SL	-0.032	-0.036	0.246^**	0.128	0.154^*	1

主成分Principal component	特征值Eigen value	贡献率 Variance contribution ratio	累计贡献率Accumulated variance contribution ratio	特征向量值 Eigen vector of measured indicators
主成分Principal component	特征值Eigen value	贡献率 Variance contribution ratio	累计贡献率Accumulated variance contribution ratio	发芽势 GV	发芽率 GR	鲜重 FW	干重 DW	根长 RL	芽长 SL
1	2.005	33.418	33.418	0.944	0.956	0.090	-0.230	0.372	-0.015
2	1.771	29.522	62.940	-0.087	-0.068	0.837	0.652	0.616	0.505
3	0.885	14.747	77.688	-0.021	-0.031	-0.154	-0.407	-0.048	0.832
4	0.763	12.723	90.411	0.216	0.157	-0.039	0.511	-0.618	0.219

Screening and comprehensive evaluation of aluminum-toxicity tolerance during seed germination in Brassca napus

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指标 Index		类群 Class
指标 Index		I	II	III	IV
AC值 AC value	发芽势GV	1.533	1.105	1.057	0.850
	发芽率GR	1.416	1.088	1.035	0.848
	鲜重FW	1.064	0.898	0.811	0.754
	干重DW	0.982	0.942	0.985	0.923
	根长RL	1.036	0.757	0.657	0.517
	芽长SL	1.161	1.140	1.026	0.933
ASF值 ASF value		0.480	0.365	0.320	0.239
WAC值 WAC value		1.178	0.969	0.907	0.786
A值 A value		1.146	0.269	-0.187	-0.788

指标 Index	关联度 Correlation degree	权重 Weight	排序 Rank
根长RL	0.313	0.213	1
鲜重FW	0.265	0.180	2
干重DW	0.246	0.168	3
芽长RL	0.218	0.149	4
发芽率GR	0.217	0.148	5
发芽势GV	0.208	0.142	6

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