Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (01): 20-30.doi: 10.3724/SP.J.1006.2020.92007

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Overexpression of OsMPK17 protein enhances drought tolerance of rice

MA Jin-Jiao1,LAN Jin-Ping1,2,ZHANG Tong1,CHEN Yue1,GUO Ya-Lu1,3,LIU Yu-Qing1,YAN Gao-Wei1,WEI Jian1,DOU Shi-Juan1,YANG Ming1,LI Li-Yun1,LIU Guo-Zhen1,*()   

  1. 1 College of Life Sciences, Hebei Agricultural University, Baoding 071001, Hebei, China
    2 Research Center for Life Sciences, Hebei North University, Zhangjiakou 075000, Hebei, China
    3 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, Guangdong, China
  • Received:2019-02-26 Accepted:2019-08-09 Online:2020-01-12 Published:2019-09-04
  • Contact: Guo-Zhen LIU E-mail:gzhliu@hebau.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31171528)

Abstract:

Mitogen-activated protein kinase (MAPK) highly conserved in eukaryotes plays important roles in stress responses in plant. In this study, full-length OsMPK17 gene was cloned and fusion protein was expressed. The purified protein was used as immunogen to generate monoclonal antibody. Western blot (WB) analyses were carried out for protein samples isolated from tissues under different abiotic stresses. The expression of OsMPK17 was induced by drought stress, suggesting that the OsMPK17 protein may play a role in drought stress response. Proteins isolated from leaves treated with abscisic acid (ABA) or methylene jasminate acid (MeJA) demonstrated a decrease of OsMPK17 protein abundance, suggesting that hormones may be involved in the function of the protein. The overexpression vector of OsMPK17 protein was established and transformed into TP309 via Agrobacteria-mediated protocol. Homozygous transgenic lines for overexpression of OsMPK17 protein were obtained. In the field planting experiment, the plant height and the spike length of transgenic lines shortened and the seed setting rate decreased. At seed germination stage, under the condition of PEG-6000 treatment, the seeds of overexpressed OsMPK17 protein lines grew better and the length of root and shoot was significantly longer than those of the wild type. At seedling stage, transgenic plants showed lower water loss rate when exposed in the air. The transgenic rice with overexpressed OsMPK17 protein grew better than the wild type in the experiment with soil drought stress and re-watering then. In conclusion, the overexpressed OsMPK17 protein enhances drought tolerance of rice. This study enhances the understanding for the function of OsMPK17 protein.

Key words: rice, MAPK protein, Western blot, stress, overexpression, antibody-based proteomics

Fig. 1

Cloning of rice OsMPK17 gene and fusion protein expression A: PCR amplification of rice OsMPK17 gene. A plasmid containing full-length OsMPK17 gene was used as template for PCR amplification of OsMPK17 gene using primers 5′-GCGGTACCATGGG CGGCCGCGCCCGCTC-3′ and 5′-GCGAGCTCGGTTTTCAGTT GAGCAAC-3′. B: Verification of recombinant pET30a-MPK17 plasmid by double digestion using Kpn I and Sac I. The PCR products and pET30a plasmid DNA were digested by Kpn I and Sac I, the ligation product was used to transform DH5α. Recombinant plasmid was verified by double digestion. C: Induction of fusion protein OsMPK17 and Coomassie blue staining. Correct pET30a- MPK17 plasmid verified by double digestion was double checked by sequencing. Sequencing verified plasmid was transformed to Codon plus bacterial strain to express fusion protein. The bacteria was cultured in LB medium containing 50 μg mL-1 kanamycin and IPTG which was added when the OD600 reached 0.6-0.8. The bacteria was collected after over night culture at 25°C and disrupted by sonication. The supernatant (S) and pellet (P) were obtained after centrifugation and total protein was separated by 10% SDS-PAGE and stained with Coomassie blue. 0: Total protein isolated at 0 time point. M: Molecular weight marker; PCR: Amplification products; K+S: Double digestion product using Kpn I and Sac I."

Fig. 2

Expression profiling of OsMPK17 protein in rice by western blot analysis A: Drought stress treatment: rice seedlings grown for 5 days were treated by 20% PEG-6000. Leaf samples were collected at 0, 1 h, 2 h, 4 h, 8 h, 12 h, 1 d, 2 d, and 3 d respectively; WB analysis were carried out for isolated total proteins. HSP: Loading control for WB analysis using HSP82 antibody. B: Treatment with hormones: leaves of rice were cultured in petri dish, 100 μmol L-1 ABA or 100 μmol L-1 MeJA was supplemented as hormone treatments. Samples were collected at 0, 6 h, 12 h, 1 d, 2 d, 3 d, 4 d, 5 d, and 6 d time points, respectively. Total proteins were isolated and analyzed by WB. HSP: Loading control for WB analysis using HSP82 antibody."

Fig. 3

Construction and identification of rice OsMPK17 overexpression vector A: PCR amplification of rice OsMPK17 gene; B: Hind III+ Xba I restriction enzyme digestion of recombinant pEASY-MPK17-3HA plasmid; C: Kpn I+ Spel I restriction enzyme digestion of recombinant pUBI-C4300-MPK17 plasmid. PCR amplification of OsMPK17 gene using plasmid containing full-length OsMPK17 cDNA as template, the primers used were 5′-GCGGTACCATGGGC GGCCGCGCCCGCTC-3′ (Kpn I restriction site was underlined) and 5′-GCGAGCTCGGTTTTCAGTTGAGCAAC-3′ (Sac I restriction site was underlined). The amplified fragment was inserted into pEASY-3HA vector and verified by double digestion. Sequence verified pEASY-MPK17-3HA was digested by Kpn I+ Spe I, the fragment was inserted into pUBI-C4300 and verified by double digestion. M: Molecular weight marker; PCR: PCR amplification product; H+X: Hind III+ Xba I restriction enzyme digestion; K+S: Kpn I+ Spel I restriction enzyme digestion."

Fig. 4

Identification of transgenic rice plants with overexpression OsMPK17 protein Upper panel: PCR product; Middle panel: WB detection of OsMPK17 protein in transgenic rice plants; Lower panel: HSP signal was used as loading control; WT: wildtype rice plants; A202, A204, A210, and A212 are independent transgenic lines; 1, 2, 3, 4, 5, 6, 7, 8, and 9 are independent plants among the same transgenic lines; PCR: PCR products; MPK17-OX: Over expressed OsMPK17 protein; MPK17-Native: the original form of OsMPK17 protein in rice."

Fig. 5

Effects of over-expressed OsMPK17 protein on the phenotype and agronomic traits of rice Photographs on the upper panel: rice whole plants and ears at mature stage of four transgenic lines and control. Bar graphs on the lower panel: plant height, spike length, seed setting rate, and tillers number of the four transgenic lines and control."

Fig. 6

Characterization of the germination trait of rice seeds with over-expressed OsMPK17 protein under drought stress A: Photographs for seeds at germination. Upper panel: control (H2O); Lower panel: drought stress (20% PEG-6000) treatment. B: Bar graphs of root and shoot lengths for seeds at germination. WT: wild type; A202, A204, A210, and A212 are transgenic lines. Experiments were carried out with three replicates; average and standard derivations were calculated. * Significant at P < 0.05. ** Significant at P < 0.01."

Fig. 7

Characterization of water loss rate of OsMPK17 protein overexpressed transgenic lines WT: wild type; A202, A204, A210, and A212 are transgenic lines. At four leaves stage, leaf blades were cut into pieces at about 3 cm, which were weighed every 30 min at room temperature (30°C). The experiment were repeated three times; the average and standard derivation were calculated."

Fig. 8

Verification of drought tolerance at seedling stage of transgenic plants over-expressed OsMPK17 protein WT: wild type; A202 and A212 were transgenic lines overexpressed OsMPK17 protein. The drought and restore experiments were carried out at seedling stage; the photographs were taken at 0 time point, eight days after drought treatment, and re-watering for 3 days."

Supplementary table 1

Transcriptional abundance comparison of OsMPK17 gene among different rice tissues"

组织 Libraries FPKM
四叶期幼苗 Seedling four-leaf stage 0.640
幼苗地上部 Shoots 0.904
20 d的叶片 20-day leaves 0.637
抽穗前花序 Pre-emergence inflorescence 3.036
抽穗后花序 Post-emergence inflorescence 6.281
花药 Anther 169.643
雌蕊 Pistil 2.371
开花后5 d种子 5 DAP seed 5.971
开花后10 d种子 10 DAP seed 10.372
开花后25 d幼胚 25 DAP embryo 7.719
开花后25 d胚乳 25 DAP endosperm 7.230
[1] Johnson G L, Lapadat R . Mitogen-activated protein kinase pathways mediated by ERK, JNK and p38 protein kinases. Science, 2002,298:1911-1912.
doi: 10.1126/science.1072682 pmid: 12471242
[2] Widmann C, Gibson S, Jarpe M B, Johnson G L . Mitogen-activated protein kinase: conservation of a three kinase module from yeast to human. Physiol Rev, 1999,79:143-180.
doi: 10.1152/physrev.1999.79.1.143 pmid: 9922370
[3] Bogre L, Meskiene I , Heberle-bors E, Hirt H. Stressing the role of MAP kinases in mitogenic stimulation. Plant Mol Biol, 2000,43:705-718.
doi: 10.1023/A:1006301614690
[4] Roberts C J, Nelson B, Marton M J, Stoughton R, Meyer M R, Bennett H A, He Y, Dai H, Walker W L, Hughes T R, Tyers M, Boone C, Friend S H . Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles. Science, 2000,287:873-880.
doi: 10.1126/science.287.5454.873 pmid: 10657304
[5] Cristina M, Petersen M, Mundy J . Mitogen-activated protein kinase signaling in plants. Annu Rev Plant Biol, 2010,61:621-649.
doi: 10.1146/annurev-arplant-042809-112252 pmid: 20441529
[6] He C, Fong S H, Yang D, Wang G L . BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice. Mol Plant-Microbe Interact, 1999,12:1064-1073.
doi: 10.1094/MPMI.1999.12.12.1064 pmid: 10624015
[7] Agrawal G K, Agrawal S K, Shibato J, Iwahashi H, Rakwal R . Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation. Biochem Biophys Res Commun, 2003,300:775-783.
doi: 10.1016/s0006-291x(02)02868-1 pmid: 12507518
[8] Shi B, Ni L, Liu Y . OsDMI3-mediated activation of OsMPK1 regulates the activities of antioxidant enzymes in abscisic acid signaling in rice. Plant Cell Environ, 2014,37:341-352.
doi: 10.1111/pce.12154
[9] Xie G, Kato H, Imai R . Biochemical identification of the OsMKK6-OsMPK3 signaling pathway for chilling stress tolerance in rice. Biochem J, 2012,443:95-102.
doi: 10.1042/BJ20111792 pmid: 22248149
[10] Xiong L, Yang Y . Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell, 2003,15:745-759.
doi: 10.1105/tpc.008714 pmid: 12615946
[11] Zhang Z, Li J, Li F, Liu H, Yang W, Chong K, Xu Y . OsMAPK3 phosphorylates OsbHLH002/OsICE1 and inhibits its ubiquitination to activate, OsTPP1, and enhances rice chilling tolerance. Dev Cell, 2017,43:731-743.
doi: 10.1016/j.devcel.2017.11.016 pmid: 29257952
[12] Wang F, Jing W, Zhang W . The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice. Plant Sci, 2014,227:181-189.
doi: 10.1016/j.plantsci.2014.08.007
[13] Hu J, Zhou J, Peng X, Xu H, Liu C, Du B, Yuan H, Zhu L, He G . The Bphi008a gene interacts with the ethylene pathway and transcriptionally regulates MAPK genes in the response of rice to brown planthopper feeding. Plant Physiol, 2011,156:856-872.
doi: 10.1104/pp.111.174334
[14] Xu R, Duan P, Yu H, Zhou Z, Zhang B, Wang R, Li J, Zhang G, Zhuang S, Lyu J, Li N, Chai T, Tian Z, Yao S, Li Y . Control of grain size and weight by the OsMKKK10-OsMKK4-OsMAPK6 signaling pathway in rice. Mol Plant, 2018,11:860-873.
doi: 10.1016/j.molp.2018.04.004 pmid: 29702261
[15] Wen J Q, Oono K, Imai R . Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice. Plant Physiol, 2002,129:1880-1891.
doi: 10.1104/pp.006072 pmid: 12177502
[16] 石佳, 杨丹丹, 葛慧雯 . 水稻OsMPK15的cDNA克隆和转录水平分析. 生物技术通报, 2018, (6):66-72.
Shi J, Yang D D, Ge H W . cDNA cloning and transcriptional level analysis of OsMPK15 in rice (Oryza sativa L.). Biotechnol Bull, 2018, (6):66-72 (in Chinese with English abstract).
[17] Lee S K, Kim B G, Kwon T R, Jeong M J, Park S R, Lee J W, Byun M O, Kwon H B, Matthews B F, Hong C B, Park S C . Overexpression of the mitogen-activated protein kinase gene OsMAPK33 enhances sensitivity to salt stress in rice (Oryza sativa L.). J Biosci, 2011,36:139-151.
doi: 10.1007/s12038-011-9002-8 pmid: 21451255
[18] Liu G Z, Pi L Y, Walker J C, Ronald P C, Song W Y . Biochemical characterization of the kinase domain of the rice disease resistance receptor-like kinase XA21. J Biol Chem, 2002,277:20264-20269.
doi: 10.1074/jbc.M110999200 pmid: 11927577
[19] Cao Y, Sun J, Zhu J, Li L, Liu G . Primer C E: designing primers for cloning and gene expression. Mol Biotechnol, 2010,46:113-117.
doi: 10.1007/s12033-010-9276-3
[20] 郭亚璐, 马晓飞, 史佳楠, 张柳, 张剑硕, 黄腾, 武鹏程, 康昊翔, 耿广荟, 陈浩, 魏健, 窦世娟, 李莉云, 尹长城, 刘国振 . 转基因水稻中CAS9蛋白质的免疫印迹检测. 中国农业科学, 2017,50:3631-3639.
doi: 10.3864/j.issn.0578-1752.2017.19.001
Guo Y L, Ma X F, Shi J N, Zhang L, Zhang J S, Huang T, Wu P C, Kang H X, Geng G H, Chen H, Wei J, Dou S J, Li L Y, Yin C C, Liu G Z . Western blot detection of CAS9 protein in transgenic rice . Sci Agric Sin, 2017,50:3631-3639 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2017.19.001
[21] Li X, Bai H, Wang X, Li L, Cao Y, Wei J, Liu Y, Liu L, Gong X, Wu L, Liu S, Liu G . Identification and validation of rice reference proteins for Western blotting. J Exp Bot, 2011,62:4763-4772.
doi: 10.1093/jxb/err084
[22] 牛东东, 郝育杰, 荣瑞娟, 韦汉福, 兰金苹, 史佳楠, 魏健, 李雪姣, 杨烁, 奚文辉 . 转基因水稻中GUS蛋白质的检测及其表达特征. 中国农业科学, 2014,47:2715-2722.
doi: 10.3864/j.issn.0578-1752.2014.14.002
Niu D D, Hao Y J, Rong R J, Wei H F, Lan J P, Shi J N, Wei J, Li X J, Yang S, Xi W H . Detection and expression of GUS protein in transgenic rice. Sci Agric Sin, 2014,47:2715-2722 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2014.14.002
[23] 张剑硕, 马金姣, 张彤, 陈悦, 魏健, 张柳, 史佳楠, 徐珊, 燕高伟, 杜铁民, 窦世娟, 李莉云, 刘丽娟, 刘国振 . 水稻蛋白质样品资源库RiceS-A300的建立与应用. 中国农业科学, 2018,51:3625-3638.
doi: 10.3864/j.issn.0578-1752.2018.19.001
Zhang J S, Ma J J, Zhang T, Chen Y, Wei J, Zhang L, Shi J N, Xu S, Yan G W, Du T M, Dou S J, Li L Y, Liu L J, Liu G Z . Establishment and application of RiceS-A300 for rice protein sample library. Sci Agric Sin, 2018,51:3625-3638 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2018.19.001
[24] Agrawal G K, Jwa N S, Rakwal R . A novel rice (Oryza sativa L.) acidic PR1 gene highly responsive to cut, phytohormones, and protein phosphatase inhibitors. Biochem Biophys Res Commun, 2000,274:157-165.
doi: 10.1006/bbrc.2000.3114 pmid: 10903912
[25] 兰金苹 . MAPK基因在Xa21介导的水稻白叶枯病抗性反应中的功能研究. 河北农业大学博士学位论文, 河北保定, 2015.
Lan J P . Function of MAPK Gene in Xa21 Mediated Resistance to Bacterial Blight in Rice. PhD Dissertation of Hebei Agricultural University, Baoding, Hebei, China, 2015 (in Chinese with English abstract).
[26] Nishimura A, Aichi I, Matsuoka M . A protocol for agrobacterium-mediated transformation in rice. Nat Prot, 2006,1:2796-2802.
doi: 10.1002/cpmb.89 pmid: 31237422
[27] Duan Y B, Zhai C Y, Li H, Li J, Mei W Q, Gui H P, Ni D H, Song F S, Li L, Zhang W G, Yang J B . An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in MeJA ponica rice (Oryza sativa L.). Plant Cell Rep, 2012,31:1611-1624.
doi: 10.1007/s00299-012-1275-3
[28] 刘巧泉, 张景六, 王宗阳, 洪孟民, 顾铭洪 . 根癌农杆菌介导的水稻高效转化系统的建立. 植物生理学报, 1998,24:259-271.
Liu Q Q, Zhang J L, Wang Z Y, Hong M M, Gu M H . Establishment of efficient transformation system of rice mediated by Agrobacterium tumefaciens. Acta Phytophysiol Sin, 1998,24:259-271 (in Chinese).
[29] Dansana P K, Kothari K S, Vij S, Tyagi A K . OsiSAP1 overexpression improves water-deficit stress tolerance in transgenic rice by affecting expression of endogenous stress-related genes. Plant Cell Rep, 2014,33:1425-1440.
doi: 10.1007/s00299-014-1626-3
[30] Lou D, Wang H, Liang G, Yu D . OsSAPK2 confers abscisic acid sensitivity and tolerance to drought stress in rice. Front Plant Sci, 2017,8:993.
doi: 10.3389/fpls.2017.00993 pmid: 28659944
[31] Chang Y, Nguyen B H, Xie Y, Xiao Y, Tang N, Zhu W, Mou T, Xiong L . Co-overexpression of the constitutively active form of OsbZIP46 and ABA-activated protein kinase SAPK6 improves drought and temperature stress resistance in rice. Front Plant Sci, 2017,8:1102.
doi: 10.3389/fpls.2017.01102 pmid: 28694815
[32] 刘国振, 刘斯奇, 吴琳, 徐宁志 . 基于抗体的水稻蛋白质组学——开端与展望. 中国科学: 生命科学, 2011,41(3):173-177.
Liu G Z, Liu S Q, Wu L, Xu N Z . Antibody-based rice proteomics-beginning and prospect. Chin Sci: Life Sci, 2011,41(3):173-177 (in Chinese).
[33] Bailey T A, Zhou X J, Chen J P, Yang Y N. Role of ethylene, abscisic acid and MAP kinase pathways in rice blast resistance. In: Wang G L, Valent B, eds. Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer, Dordrecht, 2009. pp 185-190.
[34] De V D, Yang Y, Cruz C V, Hofte M . Abscisic acid-induced resistance against the brown spot pathogenCochliobolus miyabeanus in rice involves MAP kinase-mediated repression of ethylene signaling. Plant Physiol, 2010,152:2036-2052.
doi: 10.1104/pp.109.152702 pmid: 20130100
[35] Fu S F, Chou W C, Huang D D, Huang H H . Transcriptional regulation of a rice mitogen-activated protein kinase gene, OsMAPK4, in response to environmental stresses. Plant Cell Physiol, 2002,43:958-963.
doi: 10.1093/pcp/pcf111 pmid: 12198199
[36] Kurusu T, Yagala T, Miyao A, Miyao A, Hirochika H, Kuchitsu K . Identification of a putative voltage-gated Ca 2+ channel as a key regulator of elicitor-induced hypersensitive cell death and mitogen-activated protein kinase activation in rice . Plant J, 2005,42:798-809.
doi: 10.1111/j.1365-313X.2005.02415.x pmid: 15941394
[37] Finkelstein R, Reeves W, Ariizumi T, Steber C . Molecular aspects of seed dormancy. Annu Rev Plant Biol, 2008,59:387-415.
doi: 10.1146/annurev.arplant.59.032607.092740 pmid: 18257711
[38] Kim J A, Agrawal G K, Rakwal R, Han K S, Kim K N, Yun C H, Heu S, Park S Y, Lee Y H, Jwaa N S . Molecular cloning and mRNA expression analysis of a novel rice (Oryza sativa L.) MAPK kinase kinase, OsEDR1, an ortholog of Arabidopsis At EDR1, reveal its role in defense/stress signalling pathways and development. Biochem Biophys Res Commun, 2003,300:868-876.
doi: 10.1016/s0006-291x(02)02944-3 pmid: 12559953
[39] Hoth S, Morgante M, Sanchez J P, Hanafey M K, Tingey S V, Chua N H . Genome-wide gene expression profiling inArabidopsis thaliana reveals new targets of abscisic acid and largely impaired gene regulation in the abi1-1 mutant. J Cell Sci, 2002,115:4891-4900.
doi: 10.1242/jcs.00175 pmid: 12432076
[40] Nemhauser J L, Hong F, Chory J . Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 2006,126:467-475.
doi: 10.1016/j.cell.2006.05.050 pmid: 16901781
[41] Seki M, Ishida J, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Shinozaki K Y, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K . Monitoring the expression pattern of around 7,000Arabidopsis genes under ABA treatments using a full-length cDNA microarray. Funct Integr Genomics, 2002,2:282-291.
doi: 10.1007/s10142-002-0070-6 pmid: 12444421
[42] Finkelstein R R, Gampala S S, Rock C D . Abscisic acid signaling in seeds and seedlings. Plant Cell Online, 2002,14(S1):S15-S45.
doi: 10.1021/acschembio.9b00453 pmid: 31497942
[43] Hetherington A M . Guard cell signaling. Cell, 2001,107:711-714.
doi: 10.1016/s0092-8674(01)00606-7 pmid: 11747807
[44] Zhang A, Zhang J, Ye N, Cao J, Tan M, Zhang J H, Jiang M G . ZmMPK5 is required for the NADPH oxidase-mediated self-propagation of apoplastic H2O2 in brassinosteroid-induced antioxidant defence in leaves of maize. J Exp Bot, 2010,61:4399-4411.
doi: 10.1093/jxb/erq243 pmid: 20693409
[45] Xing Y, Jia W S, Zhang J H . At MKK1 mediates ABA-induced CAT1 expression and H2O2 production via At MPK6-coupled signaling inArabidopsis. Plant J, 2008,54:440-451.
doi: 10.1111/j.1365-313X.2008.03433.x pmid: 18248592
[46] Jammes F, Song C, Shin D, Munemasab S, Takedaa K, Gua D, Choa D, Leea S, Giordoa R, Sritubtimd S, Leonhardte N, Ellisd B E, Muratab Y, Kwaka J M . MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling. Proc Natl Acad Sci USA, 2009,106:20520-20525.
doi: 10.1073/pnas.0907205106 pmid: 19910530
[47] Zong X, Li D, Gu L . Abscisic acid and hydrogen peroxide induce a novel maize group C MAP kinase gene, ZmMPK7, which is responsible for the removal of reactive oxygen species. Planta, 2009,229:485-495.
doi: 10.1007/s00425-008-0848-4
[48] Zhang S, Klessig D F . Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell, 1997,9:809-824.
doi: 10.1105/tpc.9.5.809 pmid: 9165755
[49] Seo S, Katou S, Seto H, Gomi K, Ohashi Y . The mitogen-activated protein kinases WIPK and SIPK regulate the levels of MeJAsmonic and salicylic acids in wounded tobacco plants. Plant J, 2007,49:899-909.
doi: 10.1111/j.1365-313X.2006.03003.x pmid: 17253983
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Xia, YIN Xiao-Yu, Yu Xiao-Ming, LIU Xiao-Dan. Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny [J]. Acta Agronomica Sinica, 2022, 48(5): 1191-1198.
[12] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[13] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[14] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[15] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!