Table 1

Polymorphic primers between parents"

Fig. 1

Overview of the kernel development dynamics and comparison between wild-type and mutant kernels in different days after pollination A: Ear performance of heterozygous plants; B: Comparison of dek101 and wild-type kernels at 30 days after pollination; C: Developmental dynamics of wild-type and mutant kernels in different time series; D-E: Dynamics changes in dry weight, fresh weight of 50 wild-type and dek101 kernels at different stages. F: Dynamics changes in volume of 20 wild-type and dek101 kernels at different stages. WT: wild type; dek101: mutant. Bar in A-C: 1 cm. **, indicates extremely significant difference of expression in specific tissues between dek101 and WT (P < 0.01). DAP: days after pollination."

Fig. 2

SEM observation of the starch granule between WT and dek101 kernels A-C: SEM observation of wild-type kernel of 15, 21, and 27 DAP; D-F: SEM observation of mutant type kernel of 15, 21, and 27 DAP. Bar = 5 μm."

Table 2

Segregation ratio of mutant kernels in four F2 ears"

Fig. 3

Fine mapping of dek101 n: individuals of F2 and recombinant-derived F3 population; the black vertical lines represent primary physical distance; the red numbers under InDel markers represent recombination events; the rectangular boxes represent the genotypes and phenotypes of five recombinants; the black and gray rectangles represent B73 and heterozygous genotypes, respectively."

Table 3

Candidate genes annotation in the location interval"

Fig. 4

Relative expression levels of five predicted genes in different tissues of maize inbred line B73SAM: shoot apical meristem."