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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (9): 1368-1379.doi: 10.3724/SP.J.1006.2020.94188

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mechanism of GhPIF4 regulating anther abortion under high temperature stress in cotton

CHEN Miao(), XIE Sai, WANG Chao-Zhi, LI Yan-Long, ZHANG Xian-Long, MIN Ling*()   

  1. National Key Laboratory of Crop Genetic Improvement / College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2019-11-30 Accepted:2020-03-24 Online:2020-09-12 Published:2020-04-10
  • Contact: Ling MIN E-mail:2857520681@qq.com;lingmin@mail.hzau.edu.cn
  • Supported by:
    Funding from Fundamental Research Funds for the Central Universities(140419001);National Key Research and Development Program of China(2018YFD0100403)

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Abstract:

PIF4, which belongs to the phytochrome interacting factor family, is a key transcription factor in response to temperature changes, and is widely involved in plant heat signaling transduction and hormonal signaling pathways. In this study, the GhPIF4 gene was cloned from ‘YZ1’ (Gossypium hirsutum). Subcellular localization results indicated that GhPIF4 is a nuclear located protein. qRT-PCR analysis and GUS staining of proGhPIF4:GUS transgenic cotton plants showed that the GhPIF4 gene was highly expressed in the anther, and its expression was significantly induced by high temperature (HT) in the ‘H05’ (HT sensitive line). With the overexpression of GhPIF4, different cotton transgenic lines showed different male fertility performances. Three transgenic lines with high GhPIF4 expression, OE5, OE7, and OE19, showed indehiscent anthers in the flower on the day of flowering and lower pollen activity than wild type ‘YZ1’. However, the transgenic line OE10 with slighter overexpression showed normal anther dehiscence and pollen activity. These results indicated that GhPIF4 causes anther abortion in an expression level-dependent manner. Furthermore, the IAA content was measured in OE7 and OE19 anthers at the tapetum degradation stage (9-14 mm buds) and mitotic stage (14-19 mm buds). As a result, the IAA content in OE7 and OE19 decreased compared to that in ‘YZ1’. Then, we analyzed the expression of auxin biosynthesis genes, including GhTAA1, GhYUC2, and GhCYP71A13, showing that the expression of these genes was down-regulated in OE7 and OE19, which was consistent with the decrease in IAA content. We hypothesize that overexpression of GhPIF4 might partially simulate the response of late stage anthers to HT, while constitutive overexpression of GhPIF4 may alter the auxin content in vegetative organs or early stage anthers, or the decline of IAA may lead to the anther abortion. These results provide a reference for further analysis of the function of GhPIF4 gene and understanding the mechanism of cotton anther abortion under HT.

Key words: cotton, high temperature, PIF4, auxin, anther/pollen abortion

Table 1

Vectors and strains used in the experiment"

载体/菌株 Vector/strain 用途 Purpose
pGEM-T Easy载体 pGEM-T Easy vector TA克隆 TA clone
pDONOR221载体 pDONOR221 vector BP反应 BP reaction
pGBW409载体 pGBW409 vector 35S:GhPIF4:His载体的构建 Construction of 35S:GhPIF4:His vector
pMDC43载体 pMDC43 vector GhPIF4亚细胞定位 Subcelluar localization of GhPIF4
pGWB433载体 pGWB433 vector GhPIF4启动子表达模式分析 Analysis of GhPIF4 promoter expression pattern
大肠杆菌TOP10 E. coli TOP10 载体转化 Vector transformation
农杆菌GV3101 Agrobacterium GV3101 棉花下胚轴的转化及烟草的侵染 Transformation of cotton hypocotyl and tobacco infection

Table 2

Sequence and purpose of the primers used in this study"

引物名称Primer name 引物序列Primer sequence (5°-3°) 用途Purpose
GhPIF4-CDS-F ATGGATCACCAACATGAACAACA Gene amplification
GhPIF4-CDS-R TCAGTTAAATCCCGGATTGGCAG Gene amplification
GhPIF4-CDS-BP-F GGGGACAAGTTTGTACAAAAAAGCAGGCTGGATGGAT
CACCAACATGAACAACA		 BP reaction
GhPIF4-CDS-BP-R GGGGACCACTTTGTACAAGAAAGCTGGGTGTCAGTTA
AATCCCGGATTGGCAG		 BP reaction
proGhPIF4-F TTTTTGTCTCCCATTACAGTATC Gene promoter amplification
proGhPIF4-R GGGTCATAAACTGGAAATTCAGA Gene promoter amplification
GhUB7-qRT-F CTTGACCTTCTTCTTCTTGTGCTTG qRT-PCR
GhUB7-qRT-R GAAGGCATTCCACCTGACCAAC qRT-PCR
GhPIF4-CDS-qRT-F AACTACACCTCAAAGTCCCACGG qRT-PCR
GhPIF4-CDS-qRT-R CCCGGATTGGCAGTGGTC qRT-PCR
GhTAA1-qRT-F GGTCTTAAAAAGGTTGGGGCTTA qRT-PCR
GhTAA1-qRT-R TTAGCTTGGGTATGTGTTTGATTTG qRT-PCR
GhYUC2-qRT-F ACCGATGTGGGTTTTGGCGAAT qRT-PCR
GhYUC2-qRT-R CTCAGCATTTTCCCCGGTAGCA qRT-PCR
GhCYP71A13-qRT-F CGTAAACAGACCGAAACGCAGC qRT-PCR
GhCYP71A13-qRT-R GTGGTTGCGGAAAAGAGTTCGC qRT-PCR

Fig. 1

Phylogenetic tree analysis of GhPIFs and GhPIL1s in Gossypium hirsutum and structural characteristics of GhPIF4 gene A: the phylogenetic tree analysis of GhPIFs and GhPIL1s in Gossypium hirsutum; B: comparison of amino acid sequences between GhPIF4, AtPIF4 and AtPIF5. Red line indicates bHLH domain; C: gene structure of GhPIF4. Red box and black line indicate exon and intron, respectively."

Fig. 2

Subcelluar localization of GhPIF4 35S:GFP: subcellular localization of the GFP in Nicotiana benthamiana; 35S:GhPIF4:GFP: subcellular localization of the GhPIF4:GFP fusion protein in Nicotiana benthamiana. Bar = 20 μm."

Fig. 3

Expression of GhPIF4 in different tissue and induced by high temperature in Gossypium hirsutum A and B: the expression analysis of GhPIF4 in H05 (A) and YZ1 (B), respectively. C: the expression pattern of proGhPIF4:GUS transgenic cotton; a: whole anther from the blooming flower; b: single anther from the blooming flower; c: sepal; d: petal; e: young leaf. D: the heat-induced expression analysis of GhPIF4 in ‘H05’ anthers at different development stages. Figures a, c, d, e, bar = 1 cm; figure b, bar = 100 μm; HN and HH refer to ‘H05’ under normal temperature and high temperature stress conditions, respectively; < 7 mm represents a mixture of flower buds less than 7 mm in length; **P < 0.01."

Fig. 4

Expression of GhPIF4 and Southern blotting in 35S:GhPIF4:His transgenic cottons A: the expression level of GhPIF4 in both T0 fertility plants and T0 sterility plants; B: southern blotting of T0 transgenic plants."

Fig. 5

Phenotype of 35S:GhPIF4:His transgenic cotton lines A: the whole anthers, bar = 1 cm; B: the single anther stained by TTC and observed under stereo microscope, bar = 500 μm."

Fig. 6

Content of IAA and the expression level of auxin synthesis genes in sterile anthers of GhPIF4 overexpression transgenic cottons A: the content of IAA was decreased in sterile anthers of GhPIF4 overexpression transgenic cottons; B-D: the expression level of auxin synthesis genes in sterile GhPIF4 overexpression transgenic cottons; GhTAA1 (B), GhYUC2 (C), GhCYP71A13 (D); **P < 0.01."

Fig. 7

Comparison of amino acid sequences between GhirPIF4 and GhPIF4 Red box indicates APB motif."

[1] Zhang Q, Shen B Z, Dai X K, Mei M H, Saghai Maroof M A, Li Z B. Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci USA, 1994,91:8675-8679.
pmid: 7915844
[2] Chen L, Liu Y G. Male sterility and fertility restoration in crops. Annu Rev Plant Biol, 2014,65:579-606.
doi: 10.1146/annurev-arplant-050213-040119 pmid: 24313845
[3] 袁隆平. 选育水稻光、温敏核不育系的技术策略. 杂交水稻, 1992, (1):1-4.
Yuan L P. Technical strategy for breeding rice light and temperature sensitive genic male sterile lines. Hybrid Rice, 1992, (1):1-4 (in Chinese).
[4] Endo M, Tsuchiya T, Hamada K, Kawamura S, Yano K, Ohshima M, Higashitani A, Watanabe M, Kawagishi-Kobayashi M. High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development. Plant Cell Physiol, 2009,50:1911-1922.
doi: 10.1093/pcp/pcp135 pmid: 19808807
[5] Zinn K E, Tunc-Ozdemir M, Harper J F. Temperature stress and plant sexual reproduction: uncovering the weakest links. J Exp Bot, 2010,61:1959-1968.
pmid: 20351019
[6] Dong B, Zheng X, Liu H P, Able J A, Yang H, Zhao H, Zhang M M, Qiao Y Z, Wang Y K, Liu M Y. Effects of drought stress on pollen sterility, grain yield, abscisic acid and protective enzymes in two winter wheat cultivars. Front Plant Sci, 2017,8:1008.
pmid: 28676806
[7] De Storme N, Geelen D. The impact of environmental stress on male reproductive development in plants: biological processes and molecular mechanisms. Plant Cell Environ, 2014,37:1-18.
doi: 10.1111/pce.12142 pmid: 23731015
[8] Khanna R, Huq E, Kikis E A, Al-Sady B, Lanzatella C, Quail P H. A novel molecular recognition motif necessary for targeting photoactivated phytochrome signaling to specific basic helix- loop-helix transcription factors. Plant Cell, 2004,16:3033-3044.
doi: 10.1105/tpc.104.025643 pmid: 15486100
[9] Ni M, Tepperman J M, Quail P H. Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light. Nature, 1999,400:781-784.
doi: 10.1038/23500 pmid: 10466729
[10] Huq E, Al-Sady B, Hudson M, Kim C, Apel K, Quail P H. PHYTOCHROME-INTERACTING FACTOR 1 is a critical bHLH regulator of chlorophyll biosynthesis. Science, 2004,305:1937-1941.
pmid: 15448264
[11] Huq E, Quail P H. PIF4, a phytochrome-interacting bHLH factor, functions as a negative regulator of phytochrome B signaling in Arabidopsis. EMBO J, 2002,21:2441-2450.
doi: 10.1093/emboj/21.10.2441 pmid: 12006496
[12] Leivar P, Monte E, Al-Sady B, Carle C, Storer A, Alonso J M, Ecker J R, Quail P H. The Arabidopsis phytochrome-interacting factor PIF7, together with PIF3 and PIF4, regulates responses to prolonged red light by modulating phyB levels. Plant Cell, 2008,20:337-352.
pmid: 18252845
[13] Quint M, Delker C, Franklin K A, Wigge P A, Halliday K J, van Zanten M. Molecular and genetic control of plant thermomorphogenesis. Nat Plants, 2016,2:15190.
doi: 10.1038/nplants.2015.190 pmid: 27250752
[14] Casal J J, Balasubramanian S. Thermomorphogenesis. Annu Rev Plant Biol, 2019,70:321-346.
pmid: 30786235
[15] Legris M, Klose C, Burgie E S, Rojas C C R, Neme M, Hiltbrunner A, Wigge P A, Schäfer E, Vierstra R D, Casal J J. Phytochrome B integrates light and temperature signals in Arabidopsis. Science, 2016,354:897-900.
pmid: 27789798
[16] Jung J H, Domijan M, Klose C, Biswas S, Ezer D, Gao M, Khattak A K, Box M S, Charoensawan V, Cortijo S, Kumar M, Grant A, Locke J C W, Schäfer E, Jaeger K E, Wigge P A. Phytochromes function as thermosensors in Arabidopsis. Science, 2016,354:886-889.
pmid: 27789797
[17] Box M S, Huang B E, Domijan M, Jaeger K E, Khattak A K, Yoo S J, Sedivy E L, Jones D M, Hearn T J, Webb A A R, Grant A, Locke J C W, Wigge P A. ELF3 controls thermoresponsive growth in Arabidopsis. Curr Biol, 2015,25:194-199.
doi: 10.1016/j.cub.2014.10.076 pmid: 25557663
[18] Tasset C, Singh Yadav A, Sureshkumar S, Singh R, van der Woude L, Nekrasov M, Tremethick D, van Zanten M, Balasubramanian S. POWERDRESS-mediated histone deacetylation is essential for thermomorphogenesis in Arabidopsis thaliana. PLoS Genet, 2018,14:e1007280.
pmid: 29547672
[19] Lee C M, Thomashow M F. Photoperiodic regulation of the C-repeat binding factor (CBF) cold acclimation pathway and freezing tolerance in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2012,109:15054-15059.
pmid: 22927419
[20] Liu Z J, Zhang Y Q, Wang J F, Li P, Zhao C Z, Chen Y D, Bi Y R. Phytochrome-interacting factors PIF4 and PIF5 negatively regulate anthocyanin biosynthesis under red light in Arabidopsis seedlings. Plant Sci, 2015,238:64-72.
pmid: 26259175
[21] Gangappa S N, Berriri S, Kumar S V. PIF4 coordinates thermosensory growth and immunity in arabidopsis. Curr Biol, 2017,27:243-249.
doi: 10.1016/j.cub.2016.11.012 pmid: 28041792
[22] Sun J Q, Qi L L, Li Y N, Chu J F, Li C Y. PIF4-mediated activation of YUCCA8 expression integrates temperature into the auxin pathway in regulating Arabidopsis hypocotyl growth. PLoS Genet, 2012,8:e1002594.
pmid: 22479194
[23] Franklin K A, Lee S H, Patel D, Kumar S V, Spartz A K, Gu C, Ye S Q, Yu P, Breen G, Cohen J D, Wigge P A, Gray W M. Phytochrome-interacting factor 4 (PIF4) regulates auxin biosynthesis at high temperature. Proc Natl Acad Sci USA, 2011,108:20231-20235.
pmid: 22123947
[24] Hornitschek P, Kohnen M V, Lorrain S, Rougemont J, Ljung K, Lopez-Vidriero I, Franco-Zorrilla J M, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C. Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling. Plant J, 2012,71:699-711.
pmid: 22536829
[25] Oh E, Zhu J Y, Bai M Y, Arenhart R A, Sun Y, Wang Z Y. Cell elongation is regulated through a central circuit of interacting transcription factors in the Arabidopsis hypocotyl. eLife, 2014,3:e03031.
[26] Boure N, Kumar S V, Arnaud N. The BAP Module: a multisignal integrator orchestrating growth. Trends Plant Sci, 2019,24:602-610.
doi: 10.1016/j.tplants.2019.04.002 pmid: 31076166
[27] Min L, Li Y Y, Hu Q, Zhu L F, Gao W H, Wu Y L, Ding Y H, Liu S M, Yang X Y, Zhang X L. Sugar and auxin signaling pathways respond to high-temperature stress during anther development as revealed by transcript profiling analysis in cotton. Plant Physiol, 2014,164:1293-1308.
pmid: 24481135
[28] Min L, Zhu L F, Tu L L, Deng F L, Yuan D J, Zhang X L. Cotton GhCKI disrupts normal male reproduction by delaying tapetum programmed cell death via inactivating starch synthase. Plant J, 2013,75:823-835.
doi: 10.1111/tpj.12245 pmid: 23662698
[29] Min L, Hu Q, Li Y Y, Xu J, Ma Y Z, Zhu L F, Yang X Y, Zhang X L. LEAFY COTYLEDON1-CASEIN KINASE I-TCP15-PHYT OCHROME INTERACTING FACTOR4 network regulates somatic embryogenesis by regulating auxin homeostasis. Plant Physiol, 2015,169:2805-2821.
[30] 胡海燕. 棉花茉莉酸信号负调控因子GhJAZ2的克隆及功能分析. 华中农业大学博士学位论文, 湖北武汉, 2016.
Hu H Y. The Cloning and Functional Characterization of a Cotton Negative Regulater GhJAZ2 in Jasmonic Acid Pathway. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2016 (in Chinese with English abstract).
[31] Jin S X, Zhang X L, Nie Y C, Guo X P, Liang S G, Zhu H G. Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biol Plant, 2006,50:519-524.
[32] Wu Y L, Min L, Wu Z C, Yang L, Zhu L F, Yang X Y, Yuan D J, Guo X P, Zhang X L. Defective pollen wall contributes to male sterility in the male sterile line 1355A of cotton. Sci Rep, 2015,5:9608.
doi: 10.1038/srep09608 pmid: 26043720
[33] Yao X, Tian L, Yang J, Zhao Y N, Zhu Y X, Dai X, Zhao Y, Yang Z N. Auxin production in diploid microsporocytes is necessary and sufficient for early stages of pollen development. PLoS Genet, 2018,14:e1007397.
doi: 10.1371/journal.pgen.1007397 pmid: 29813066
[34] Feng X L, Ni W M, Elge S, Mueller-Roeber B, Xu Z H, Xue H W. Auxin flow in anther filaments is critical for pollen grain development through regulating pollen mitosis. Plant Mol Biol, 2006,61:215-226.
doi: 10.1007/s11103-006-0005-z pmid: 16786302
[35] Sun J Q, Qi L L, Li Y N, Zhai Q Z, Li C Y. PIF4 and PIF5 transcription factors link blue light and auxin to regulate the phototropic response in Arabidopsis. Plant Cell, 2013,25:2102-2114.
doi: 10.1105/tpc.113.112417 pmid: 23757399
[36] Cheng Y F, Dai X H, Zhao Y D. Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. Genes Dev, 2006,20:1790-1799.
pmid: 16818609
[37] Tadashi S, Takeshi O, Shinya M, Mari T, Yuta T, Nahoko H, Yutaka M, Hideyuki T, Masao W, Atsushi H. Auxins reverse plant male sterility caused by high temperatures. Proc Natl Acad Sci USA, 2010,107:8569-8574.
doi: 10.1073/pnas.1000869107 pmid: 20421476
[38] Cecchetti V, Altamura M M, Brunetti P, Petrocelli V, Falasca G, Ljung K, Costantino P, Cardarelli M. Auxin controls Arabidopsis anther dehiscence by regulating endothecium lignification and jasmonic acid biosynthesis. Plant J, 2013,74:411-422.
pmid: 23410518
[39] Cecchetti V, Altamura M M, Falasca G, Costantino P, Cardarelli M. Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. Plant Cell, 2008,20:1760-1774.
doi: 10.1105/tpc.107.057570 pmid: 18628351
[40] Goldberg R B, Beals T P, Sanders P M. Anther development: basic principles and practical applications. Plant Cell, 1993,5:1217-1229.
doi: 10.1105/tpc.5.10.1217 pmid: 8281038
[41] Yang C Y, Xu Z Y, Song J, Conner K, Vizcay Barrena G, Wilson Z A. Arabidopsis MYB26/MALE STERILE35 regulates secondary thickening in the endothecium and is essential for anther dehiscence. Plant Cell, 2007,19:534-548.
doi: 10.1105/tpc.106.046391 pmid: 17329564
[42] Stepanova A N, Robertson-Hoyt J, Yun J, Benavente L M, Xie D Y, Dolezal K, Schlereth A, Jurgens G, Alonso J M. TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. Cell, 2008,133:177-191.
doi: 10.1016/j.cell.2008.01.047 pmid: 18394997
[43] Muller T M, Bottcher C, Morbitzer R, Gotz C C, Lehmann J, Lahaye T, Glawischnig E. TRANSCRIPTION ACTIVATOR- LIKE EFFECTOR NUCLEASE-mediated generation and metabolic analysis of camalexin-deficient cyp71a12 cyp71a13 double knockout lines. Plant Physiol, 2015,168:849-858.
doi: 10.1104/pp.15.00481 pmid: 25953104
[44] Nagpal P, Ellis C M, Weber H, Ploense S E, Barkawi L S, Guilfoyle T J, Hagen G, Alonso J M, Cohen J D, Farmer E E, Ecker J R, Reed J W. Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation. Development, 2005,132:4107-4118.
doi: 10.1242/dev.01955 pmid: 16107481
[45] Wu M F, Tian Q, Reed J W. Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development, 2006,133:4211-4218.
pmid: 17021043
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