320份蚕豆蛋白质含量的SSR关联分析

doi:10.3724/SP.J.1006.2024.44013

Abstract

Abstract:

Faba bean (Vicia faba L.) is an important crop known for its high protein content, which includes all eight essential amino acids in a balanced manner. Mining protein-related genes in faba bean not only contributes to the breeding of high-quality protein varieties but also holds great significance for meeting the future demand of plant protein. In this study, a total of 320 faba bean germplasms were evaluated for protein content across three environments (Xining, Huzhu, and Huangyuan in Qinghai province) over one year. Additionally, 132 selected SSR makers were used to conduct association analysis. The results showed that the protein content ranged from 19.51% to 54.34%. The coefficients of variation for the three environments were 10.835, 20.865, and 13.380, respectively, displaying a normal distribution with phenotypic diversity. Among the 320 materials, 778 polymorphic loci were detected by the 132 markers. The average allele number was 6, with a range of 3 to 12. The polymorphism information content (PIC) varied from 0.1527 (V1797) to 0.8225 (SSR-12192), with a mean value of 0.5583. Markers with a PIC value greater than the mean accounted for 49.24% of the total markers, indicating relatively high genetic diversity among the selected markers. Genetic structure analysis showed that the 320 materials could be divided into two subgroups. Subgroup I consisted of 176 materials, subgroup II contained 107 materials, while the remaining 37 materials did not exhibit distinct group affiliation, representing a mixed taxon. Overall, the genetic diversity of the marker genes was high, and the test population displayed relatively homogeneous structure. Through GLM and MLM analyses, a total of 22 SSR markers significantly correlated with protein content were identified. Notably, three SSR markers (SSR-10894, SSR-12695, V1929) displayed a strong association with protein content in this study. Among them, SSR-13584 exhibited consistent correlation across multiple environments, with an explanatory rate ranging from 4.07% to 5.19%. V1929 showed a high correlation with protein content in Huangyuan county, with explanatory rate of 10.00% and 9.20% using both correlation analysis models. The findings of this study provide a theoretical basis for parental selection, faba bean protein-related gene mining, and quality breeding.

Key words: faba bean, protein content, SSR marker, association analysis

CHEN Zhi-Kai, ZHOU Xian-Li, ZHANG Hong-Yan, TENG Chang-Cai, HOU Wan-Wei. SSR association analysis of the protein content of 320 faba bean germplasms[J].Acta Agronomica Sinica, 2024, 50(11): 2775-2786.

Figures/Tables 7

Table 1

Fig. 1

Table 2

Polymorphism statistics of 132 pairs of SSR markers"

标记 Marker	等位基因数 Allele number	引物多态性 PIC	标记 Marker	等位基因数 Allele number	引物多态性 PIC
SSR-1914	3	0.4606	V2704	5	0.3585
EST-475	3	0.2997	V2772	5	0.4886
SSR-11967	3	0.2496	V734	5	0.6544
V1846	3	0.3888	V3051	5	0.6373
V2949	3	0.2344	SSR-17892	5	0.3478
SSR-12526	3	0.4047	V2937	5	0.3659
V1916	3	0.4585	SSR-17611	5	0.4910
V2276	4	0.5420	SSR-12751	5	0.6884
V3027	4	0.4247	SSR-12911	5	0.3705
V2268	4	0.6244	SSR-12503	5	0.3990
V2253	4	0.4972	SSR-10832	5	0.3934
SSR-14892	4	0.5657	SSR-17506	5	0.5209
SSR-13508	4	0.5550	SSR-10927	5	0.5238
V3056	4	0.5666	SSR-17488	5	0.4527
V2979	4	0.4306	SSR-12180	5	0.5016
V2716	4	0.3119	C-3165	5	0.5332
V2733	4	0.5227	EST-657	5	0.4409
V2930	4	0.4777	V1761	5	0.4919
SSR-13844	4	0.4646	V2574	5	0.4212
SSR-11854	4	0.5819	V2073	5	0.6596
SSR-15868	4	0.3683	SSR-18329	5	0.5045
V2888	4	0.2067	FBES0135	5	0.6638
SSR-11862	4	0.4002	V1133	5	0.5655
SSR-10757	4	0.4669	V2729	5	0.6013
V1702	4	0.4414	SSR-14219	5	0.6894
FBFS0809	4	0.5123	SSR-13380	5	0.6906
V1778	4	0.4061	SSR-16885	5	0.5965
V1797	4	0.1527	V2228	5	0.4234
V1446	4	0.4099	SSR-14430	6	0.6116
SSR-10894	4	0.6049	V2109	6	0.4384
SSR-18215	4	0.3473	Y240	6	0.6954
V2103	4	0.5416	V2138	6	0.5981
V2275	5	0.4658	V2687	6	0.4160
V3035	5	0.6973	V2991	6	0.6952
V2720	6	0.5274	SSR-17507	7	0.7231
V2731	6	0.5199	SSR-11885	7	0.5577
SSR-13584	6	0.6008	SSR-12695	7	0.4231
SSR-11723	6	0.6720	V-2460	7	0.6912
SSR-13067	6	0.5097	SSR-12066	7	0.5415
SSR-16047	6	0.4274	V1929	7	0.5325
SSR-12332	6	0.5576	V2542	7	0.5381
SSR-11448	6	0.7125	C-3883	7	0.6247
SSR-11365	6	0.4309	SSR-12842	7	0.6006
SSR-11621	6	0.6427	V2280	7	0.7914
EST-868	6	0.7039	Y67	8	0.6180
V2493	6	0.5235	SSR-3581	8	0.5274
V1693	6	0.5982	V2986	8	0.6891
SSR-16592	6	0.6966	V3017	8	0.7695
V2958	6	0.5727	V3043	8	0.7295
V2653	6	0.5367	T179	8	0.6833
SSR-13593	6	0.5062	V2900	8	0.7378
EST-24	6	0.5812	SSR-11342	8	0.6871
V1836	6	0.6622	SSR-12667	8	0.5764
FBES0142	6	0.6286	V1820	8	0.6976
V2388	6	0.6539	T135	9	0.7005
V2188	7	0.5414	V2220	9	0.6832
V2285	7	0.5948	SSR-14109	9	0.6387
V3052	7	0.6625	V3012	9	0.6169
V2970	7	0.5093	SSR-12586	9	0.7243
SSR-14765	7	0.7551	V2646	9	0.7528
V2873	7	0.6993	V3031	10	0.8132
V2882	7	0.7333	V2698	10	0.6921
V2745	7	0.5539	V2015	10	0.7787
V2277	7	0.4044	V3058	11	0.7450
V3053	7	0.7675	SSR-12192	11	0.8225
SSR-14447	7	0.7575	T19	12	0.7291

Table 2

Fig. 2

Fig. 3

Fig. 4

Table 3

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地点 Location	最小值 Minimum value (%)	最大值 Maximum value (%)	平均数 Average (%)	标准偏差 Standard deviation	变异系数 Coefficient of variation (%)	方差 Variance	偏度 Skewness	峰度 Kurtosis
西宁Xining	23.602	41.218	32.917	3.292	10.00	10.835	0.123	-0.163
互助Huzhu	19.157	54.348	34.599	4.568	13.20	20.865	0.142	0.630
湟源Huangyuan	24.937	47.011	34.788	3.659	10.51	13.380	0.079	0.271

表型 Phenotype	标记 Marker	显著性 Significance	解释率 Interpretation rate	显著性 Significance	解释率 Interpretation rate
蛋白质含量(西宁) Protein content (Xining)	EST-475	0.0443	0.0204
	SSR-11967	0.0366	0.0207
	V2704	0.0389	0.0370
	SSR-13584	0.0463	0.0455
	SSR-14447	0.0321	0.0531
	V2882	0.0066	0.0537
	SSR-13593	0.0219	0.0608	0.0285	0.0528
	SSR-17892	0.0059	0.0455	0.0042	0.0430
	V2285	0.0465	0.0319
	V2882	0.0101	0.0453
蛋白质含量(互助) Protein content (Huzhu)	EST-868	0.0489	0.0345
	V3035	0.0200	0.0409	0.0296	0.0349
	V2388	0.0065	0.0562	0.0120	0.0480
	EST-657	0.0356	0.0411	0.0429	0.0369
	SSR-13584	0.0367	0.0519	0.0462	0.0464
	SSR-12192	0.0124	0.0781	0.0146	0.0712
	SSR-12751	0.0086	0.0410	0.0083	0.0385
蛋白质含量(湟源) Protein content (Huangyuan)	V2704	0.0189	0.0458	0.0221	0.0414
	SSR-12180	0.0358	0.0463
	V2745	0.0438	0.0493
	SSR-13584	0.0413	0.0504
	EST-657	0.0057	0.0555	0.0064	0.0509
	SSR-10894	0.0036	0.0534	0.0039	0.0493
	SSR-11448	0.0230	0.0570	0.0284	0.0513
	V2073	0.0200	0.0624	0.0333	0.0537
	V2493	0.0104	0.0632	0.0181	0.0542
	SSR-11885	0.0414	0.0694	0.0469	0.0633
	SSR-12695	0.0042	0.0717	0.0035	0.0684
	V1929	0.0001	0.1000	0.0001	0.0920
	SSR-12586	0.0023	0.1164	0.0011	0.1108
	V1846	0.0023	0.0416	0.0025	0.0385
	SSR-1914	0.0422	0.0151	0.0492	0.0131
	SSR-12751	0.0491	0.0275
	V734	0.0486	0.0279
	V2574	0.0350	0.0304	0.0344	0.0285
	V2772	0.0183	0.0404	0.0183	0.0377
	T19	0.0471	0.0611

SSR association analysis of the protein content of 320 faba bean germplasms

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