Abstract:

Empty pods and ill-filled pods leading to low yield in peanut production resulted from deficient calcium has long been an existing problem in the red and yellow soil regions in the south China. Based on the measurements and comparisons in field experiments with different levels of calcium, the seven differentially expressed proteins which related to the development /abortion embryos of peanut were got by 2-D electrophoresis, MALDI-TOF-MS and protein databases. The results searched from the protein databases were as follows: 4^# protein, which up-regulated in embryos from deficient calcium group, showed high homology with the 70 kD protein from the chloroplast envelope membrane of Spinacia oleracea L; 6^# protein disappeared in embryos from deficient calcium group showed high homology with the β-chain of the (ATPB) ATP synthase from Hevea brasiliensis L; and 7^# protein also disappeared in embryos from deficient calcium group was considered similar to the Actin 97&100 from Solanum tuberosum,etc. All the results indicated that the molecular mechanism of embryo abortion mediated by calcium may be relevant to some genes controlling energy transferring（ATP synthesis）, oxidoreduction system, endo-membrane system and cytoskeleton structure system which are transcripted or expressed unnomally.

Key words: Embryo abortion, Embryo development, Calcium, Protein, Peanut（Arachis hypogaea L.）