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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 66-70.doi: 10.3724/SP.J.1006.2009.00066

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Analysis of Hemoglobin Gene in Cyanobacterium and Transformation into Brassica napus L.

TAN Xiao-Li1,2,KONG Fan-Ming1,*,ZHANG Li-Li1,CHEN Song2,QI Chui-Kou2*   

  1. 1Institute of Life Sciences,Jiangsu University,Zhenjiang 212013,China;2 Institute of Imdustrial Crop,Jiangsu Academy of Agricultural Sciences,Nanjiang 200014,China
  • Received:2008-03-24 Revised:2008-07-16 Online:2009-01-12 Published:2008-11-17
  • Contact: QI Chui-Kou

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Abstract:

Hemoglobin exists in most organisms including animals, plants, protozoa, algae, bacteria and fungi. In the paper, hemoglobin gene SLR2097 was cloned from Synechocystis sp. PCC 6803, which is 375 bp in size and encodes123 amino acid polypeptide chains belonging to the truncated hemoglobin family. Hemoglobin canbind oxygen and co-ordinate the heme iron with protein ligation. Database search showed that a motif ‘F-[L]-x(4)-[G]-G-[T]-x(2)-[Y]-x-[G]-[R]-x-[M]-x(3)-H’ occurred in hemoglobins. Therefore, the truncated hemoglobins might evolve from the same ancestor of hemoglobin. The hemoglobin gene SLR2097 was ligated into binary vector pCAMBIA1300-CaMV35S, and then transformed into rapeseed cultivar Ningyou 12 with Agrobacterium mediated transformation method. Phenotype analysis indicated that the expression of hemoglobin gene SLR2097 led to the earlier maturity of transgenic rapeseed. These phenotype variations have a potential application in the rapeseed production in the future.

Key words: Agrobacterium tumefaciens, Brassica napus, Hemoglobin SLR2097, Transformation

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