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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 173-178.doi: 10.3724/SP.J.1006.2009.00173

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Analvsis of Sichuan Sun-Cured Tobacco Germplasm by SRAP

LONG Teng1,LIU Lei2,HUANG Yu-Bi1.2.*   

  1. 1Maize Research Institute of Sichuan Agricultural University;2Agronomy College of sichuan Agricultural University,Ya'an 625014,China
  • Received:2008-03-28 Revised:2008-07-13 Online:2009-01-12 Published:2008-11-18
  • Contact: HUANG Yu-Bi

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Abstract:

SRAP was used to analyze genetic relationships of 25 sun-cured tobacco varieties, one Nicotiana rustica variety, and one flue-cured tobacco variety collected from Sichuan province. PCR products were detected by polyacrylamide gel electrophoresis (PAGE). A total of 3 368 DNA fragments were obtained, of which 998 were specific bands (polymorphic ratio was 29.6%), the average number of amplified DNA bans was 40 per primer．Genetic similarity ranged from 0.26 to 0.97 for all varieties, from 0.38 to 0.78 between Nicotiana rustica varieties, flue-cured tobacco varieties and sun-cured tobacco varieties, and from 0.75 to 0.97 among 25 Sun-cured tobacco varieties. Cluster analysis was carried out with unweighted pair group method with arithmetic mean (UPGMA)．The results showed that all 27 varieties could be clustered into three groups, which revealed the genetic diversity among three cultivar-type tobacco germplasms. Furthermore, 25 sun-cured tobacco varieties could be classified into three subgroups and two single varieties. Maoyan and Liuyan were two traditional famous groups of Sichuan sun-cured tobacco species. However, Maoyan and Liuyan varieties named by traditional method were not obviously grouped into two types. All results indicated that SRAP analysis may be used to reveal the genetic relationships among sun-cured tobacco varieties, providing a scientific basis for tobacco genetic research and breeding.

Key words: Sun-cured tobacco, Germplasm, Genetic relationship, SARP

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