马铃薯晚疫病抗性基因R11的遗传定位

doi:10.3724/SP.J.1006.2009.00992

Abstract

Abstract:

Late blight caused by Phytophthora infestans is the most destructive disease for potato (Solanum tuberosum). A F₁ segregating population consisting of 83 genotypes, derived from R11 differential MaR11 and the susceptible potato cultivar Katahdin, was assessed for late blight resistance in detached leaves assay and genetically analyzed. The results showed that the distribution for R11 was clearly bimodal with the two peaks coinciding with the blight scores of the MaR11 (the resistant parent) and Katahdin (the susceptible parent), and the observed segregation with 39 resistant and 44 susceptible plants fitted a 1:1 ratio. It indicated that _R11 was inherited as a major dominant R gene and presented in the simplex condition in MaR11. There was a major late blight resistance locus (MLB) in potato on the long arm of chromosome 11, where R11 showed allelic versions of the R3 and R10 loci. Using the combination of comparative mapping and bulked segregant analysis, we developed six markers linked to R11 which was located on the end of the long arm of chromosome 11 with a distance of about 2.4 cM to marker C2_At5g59960. R11 was telomeric to the R3a and R10 gene. The genetic map constructed in this paper provides a basis for further construction of high-resolution genetic map of the R11 gene.

Key words: Potato, Late blight, Rll, Genetic mapping

XU Jian-Fei,HUANG San-Wen,JIN Li-Ping,DUAN Shao-Guang,QU Dong-Yu. Genetic Mapping of Rll Gene Conferring Resistance to Late Blight in Potato(Solanum tuberosum)[J].Acta Agron Sin, 2009, 35(6): 992-997.

References

[1] McDonald B A, Linde C. Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol, 2002, 40: 349-379

[2] Andrivon D, Lucas J M, Ellissèche D. Development of natural late blight epidemics in pure and mixed plots of potato cultivars with different levels of partial resistance. Plant Pathol, 2003, 52: 586-594
[3] Dangl J L, Jones J D. Plant pathogens and integrated defense responses to infection. Nature, 2001, 411: 826-833

[4] Mundt C C. Use of multiline cultivars and cultivar mixtures for disease management. Annu Rev Phytopathol, 2002, 40: 381-410

[5] Ballvora A, Ercolano M R, Weiss J, Meksem K, Bormann C A, Oberhagemann P, Salamini F, Gebhardt C. The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant J, 2002, 30: 361-371

[6] Huang S, van der Vossen E A, Kuang H, Vleeshouwers V G, Zhang N, Borm T J, van Eck H J, Baker B, Jacobsen E, Visser R G. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant J, 2005, 42: 251-261

[7]Song J, Bradeen J M, Naess S K, Raasch J A, Wielgus S M, Haberlach G T, Liu J, Kuang H, Austin-Phillips S, Buell C R, Helgeson J P, Jiang J. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci USA, 2003, 100: 9128-9133

[8] Huang S. Discovery and characterization of the major late blight resistance complex in potato: Genomic structure, functional diversity, and implications. Wageningen Netherlands: Wageningen Universiteit (Wageningen University), 2005

[9] Bradshaw J E, Bryan G J, Lees A K, McLean K, Solomon-Blackburn R M. Mapping the R10 and R11 genes for resistance to late blight (Phytophthora infestans) present in the potato (Solanum tuberosum) R-gene differentials of black. Theor Appl Genet, 2006, 112: 744-751

[10] Vleeshouwers V G, van Dooijeweert W, Keizer L C P, Sijpkes L, Govers F, Colon L T. A laboratory assay for Phytophthora infestans resistance in various Solanum species reflects the field situation. Eur J Plant Pathol, 1999, 105: 241-250

[11] van der Lee T, Testa A, van’t Klooster J, van den Berg-Velthuis G, Govers F. Chromosomal deletion in isolates of Phytophthora infestans correlates with virulence on R3, R10, and R11 potato lines. Mol Plant Microbe Interact, 2001, 14: 1444-1452
[12] Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman j, Kuiper M, Zabeau M. AFLP: A new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[13] Malcolmson J F, Black W. New R-genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica, 1966, 15: 199-203
[14] Flor H H. Current status of the gene-for-gene concept. Annu Rev Phytopathol, 1971, 9: 276-296
[15] Xu J-F(徐建飞), Wang J-J(王加加), Li Y(李颖), Jin L-P(金黎平), Jacobsen E, Huang S-W(黄三文), Qu D-Y(屈冬玉). Genetic mapping of the R10 gene for resisting late blight in potato by comparative genomics. Sci Technol Rev (科技导报), 2007, 25: 30-35(in Chinese with English abstract)
[16] Harrison J G, Lowe R. Stability of R-gene resistance to late blight in potato leaves. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. 365-367
[17] Turkensteen L J. Interaction of R-genes in breeding for resistance of potatoes against Phytophthora infestans. In: Rep of the Planning Conf: Fungal Diseases of the Potato. Lima: International Potato Center, 1989. pp 85-96
[18] Stewart H E, Gourlay F. Recognising race-specific resistance to Phytophthora infestans. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. pp 255-260
[19] Brouwer D J, Jones E S, St. Clair D A. QTL analysis of quantitative resistance to Phytophthora infestans (late blight) in tomato and comparisons with potato. Genome, 2004, 47: 475-492
[20] Li X, van Eck H J, Rouppe van der Voort J N A M, Huigen, D J, Stam P, Jacobsen E. Autotetraploids and genetic mapping using common AFLP markers: The R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet, 1998, 96: 1121-1128
[21] Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C. The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet,1992, 233: 278-283
[22] Huang S, Vleeshouwers V G A A, Werij J S, Hutten R C B, van Eck H J, Visser R G F, Jacobsen E. The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant Microbe Interact,2004, 17: 428-435
[23] Naess S K, Bradeen J M, Wielgus S M, Haberlach G T, McGrath J M, Helgeson J P. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet,2000, 101: 697-704
[24] Colon L T, Budding D J. Hoogendoorn J. Breeding for foliar resistance to Phytophthora infestans in potato: the influence of test conditions on the results of screening for field resistance. In: Dowley L J, Bannon E, Cooke L R, Keane T, O’Sullivan E, eds. Phytophthora infestans. Dublin: Boole Press Ltd, 1995. pp 282-288
[25] Meyers B C, Chin D B, Shen K A, Sivaramakrishnan S, Lavelle D O, Zhang Z, Michelmore R W. The major resistance gene cluster in lettuce is highly duplicated and spans several megabases. Plant Cell, 1998, 10: 1817-1832

Related Articles 15

[1]	Chen Guo-Huan, Zhang Rui, Li Yan-Di, Zhao Jia-Qi, Ren Yong-Tao, Zhang Tian-Ci, Guo Hua-Chun, Li Jun, Yang Fang. Effects of foliar application of exogenous selenium on anthocyanin biosynthesis in tubers of light purple-fleshed potatoes [J]. Acta Agronomica Sinica, 2026, 52(6): 1876-1890.
[2]	Tian Li-Tao, Ding Ning, Wang Shu-Lin, Qi En-Fang, Zhang Rong, Wang Rui-Rui, Ma Li-Wen, Li Jian-Wu, Yang Jiang-Wei. Genome-wide identification of the Argonaute gene family and its induction by late blight in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2026, 52(4): 1116-1126.
[3]	Jiang Jia-Hui, Jiang Bing-Zhi, Liu Guan-Ming, Wang Zhang-Ying, Tang Chao-Chen. Establishment and optimization of near-infrared spectroscopy models for quality traits of purple-fleshed sweet potato [J]. Acta Agronomica Sinica, 2026, 52(4): 1088-1102.
[4]	Wang Yi-Han, Li Fu-Chang, Liu Yi, Zhu Guo-Peng. Cloning of the IbOPR2 gene promoter and identification of regulatory factors in sweetpotato [J]. Acta Agronomica Sinica, 2026, 52(4): 1268-1276.
[5]	Wang Ling, Hu Hao, Song Jia-Feng, Cheng Jie-Lan, Chen Ying, Zheng Ting-Ting, Lyu Zhao-Yan, Zhu Xiao-Biao, Hou Hua-Lan. Cloning and functional validation of UDP-glycosyltransferase gene StUGT52 in potato [J]. Acta Agronomica Sinica, 2026, 52(3): 665-676.
[6]	Zhang Yu, Liu Fang, Cai Cheng-Cheng, Yang Xiao-Hua, Jia MO-Shi-Zha, Yang Yuan-Jun, Wang Xi-Yao. Preliminary investigation on the mechanism of potato tuber dormancy release induced by combined treatment of bromoethane and gibberellin [J]. Acta Agronomica Sinica, 2026, 52(3): 825-838.
[7]	Yu Yong-Chao, Liu Ming, Jin Rong, Zhao Peng, Zhang Qiang-Qiang, Wang Jing, Zhu Xiao-Ya, Tang Zhong-Hou. Physiological mechanism and transcriptome analysis of sweet potato overgrowth under high-nitrogen conditions [J]. Acta Agronomica Sinica, 2026, 52(3): 813-824.
[8]	Xu Qiang, Xie Kui-Zhong, Hu Xin-Yuan, Yue Yun, Dong Bo, Luo Ai-Hua. Effects of continuous cropping on the structure and function of soil nematode communities in potato [J]. Acta Agronomica Sinica, 2026, 52(2): 527-538.
[9]	Yang Xuan, Li Jian-Kang, He Wan-Jie, Li You-Jun, Cheng Xiang-Han, Hou Wen-Bang. Effects of selenium fertilization on fresh-cut browning in sweet potatoes and its mechanism analysis [J]. Acta Agronomica Sinica, 2026, 52(2): 578-589.
[10]	Yang Biao, Du Shuai-Kang, Zhang Ji-Wang, Shi Ying, Zhang Li-Li. Genome-wide identification of class III POD gene family in potato and its expression profile analysis [J]. Acta Agronomica Sinica, 2026, 52(2): 405-420.
[11]	Ji Xuan-Tong, Bian Chun-Song, Jin Li-Ping, Li Sen, Qin Jun-Hong, Li Guang-Cun. Responses of root-associated microorganisms of different drought-tolerant potato varieties to drought conditions [J]. Acta Agronomica Sinica, 2026, 52(1): 165-177.
[12]	Tian Jia-Chun, Ge Xia, Li Shou-Qiang, Li Mei, Tian Shi-Long, Zhang Ya-Qian, Cheng Jian-Xin, Li Yu-Mei. Mechanism of low O₂and high CO₂ storage environment delaying aging of potato tuber [J]. Acta Agronomica Sinica, 2026, 52(1): 262-278.
[13]	Wang Ya-Zhi, Yang Biao, Ji Xiang-Lin, Shi Ying, Zhang Li-Li. Identification of drought-resistant resources and preliminary screening of drought resistant genes in diploid potatoes [J]. Acta Agronomica Sinica, 2026, 52(1): 72-84.
[14]	ZHUO Feng-Qi, TANG Zhen-San, LEI Yu-Jun, CHENG Li-Xiang, ZHAO Tian-Tian, LYU Tai, YANG Chen, ZHANG Feng. Screening of low glycemic potato varieties (lines) based on cooking methods and regeneration temperature [J]. Acta Agronomica Sinica, 2025, 51(9): 2538-2546.
[15]	ZHU Jin-Cheng, YANG Qiu-Hua, CHENG Li-Xiang, LI Wen-Li, SHI Ming-Ming, LI Hui-Xia, ZHANG Feng. Screening of potato germplasm for resistance to Meloidogyne incognita and analysis of related physiological responses [J]. Acta Agronomica Sinica, 2025, 51(9): 2307-2317.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Genetic Mapping of Rll Gene Conferring Resistance to Late Blight in Potato(Solanum tuberosum)

PDF

Cited