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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 998-1005.doi: 10.3724/SP.J.1006.2009.00998

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Mapping of Powdery Mildew Resistance Gene MlWE29 in Wheat Originated from Wild Emmer(Triticum turgidum var.dicoccoides)

ZHANG Lian-Song,HUA Wei,GUAN Hai-Ying,LI Gen-Qiao,ZHANG Hong-Tao,XIE Chao-Jie,YANG Zuo-Min,SUN Qi-Xin,LIU Zhi-Yong*   

  1. Department of Plant Genetics & Breeding,China Agricultural University/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Resarch & Utilization,Ministry of Education,Beijing 100094,China
  • Received:2008-12-22 Revised:2009-01-05 Online:2009-06-12 Published:2009-04-16
  • Contact: LIU Zhi-Yong，E-mail:zhiyongliu@cau.edu.cn

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Abstract:

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in many regions in the world. Breeding for the resistance is the most economical and effective method for controlling this disease. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and has been proven to be an important resource of disease resistance improvement. In this study, a common wheat line 3D258 with powdery mildew resistance was developed by crossing wild emmer accession WE29 with common wheat landrace Yanda1817 and backcrossing with common wheat line 87-1 (Yanda 1817/WE29//5*87-1, F6). Genetic analysis of the F2 population and their F3 families, developed from 3D258 and a susceptible common wheat cultivar Xuezao, indicated that the powdery mildew resistance in 3D258 was controlled by a single dominant gene, designated temporarily as MlWE29. Molecular markers and the bulked segregant analysis were used to characterize the powdery mildew resistance gene MlWE29. Six SSR markers (Xgwm335, Xgwm213, Xgwm639, Xwmc415, Xwmc289, and Xwmc75) and five EST-STS markers (BE494426, BE442763, CD452476, BE445282, and BE407068) were found to be linked to MlWE29. Using Chinese Spring nullisomic-tetrasomics, ditelosomics and deletion lines, MlWE29 was physically mapped on chromosome 5BL bin 0.59–0.79. The common wheat line 3D258 and its resistance gene linked molecular markers could be used in disease resistance genes pyramiding and marker-assisted selection in wheat breeding program.

Key words: Blumeriagraminis f.sp.tritici, Triticum turgidum var.dicoccoides, Powdery mildew resistance gene, Molecular markers


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