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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1318-1323.doi: 10.3724/SP.J.1006.2010.01318

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs for Lint Percentage and Seed Index by Using Gossypium barbadense Chromosome Segment Introgression Lines

 ZHU Ya-Juan,WANG Peng**,GUO Wang-Zhen, ZHANG Tian-Zhen*   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-11-02 Revised:2010-04-20 Online:2010-08-12 Published:2010-05-20
  • Contact: ZHANG Tian-Zhen, E-mail: cotton@njau.edu.cn; Fax: 025-84395307

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Abstract: Seed index (SI) and lint percentage (LP) are very important components, and many researchers have done abundant investigation on them by using different populations. Chromosome segment introgression lines (CSIL), different from the recurrent parent in introgressed segments, are the ideal materials for QTL research and the core of the breeding by design. The F2 and F2:3 populations were developed from CSILs-IL-15-5 and IL-15-5-1, and used in mapping QTLs for lint percentage and seed index with SSR markers. The SI and LP characteristics of the 774 F2 individuals and F2:3 families were analyzed by composite interval mapping (CIM), and one QTL was detected for SI and two QTLs for LP. qSI-15-1 was located between NAU2814 and NAU3040 with confident genetic distance of 6.70 cM in the F2 population and between NAU3040 and JESPR152 with confident genetic distance of 5.70 cM in the F2:3 population. qLP-15-1 detected in the F2 and F2:3 populations was located between NAU3040 and JESPR152 with confident genetic distance of 5.40 cM and 3.20 cM respectively; qLP-15-2 was mapped between NAU5302 and NAU2901 with confident genetic distance of 0.08 cM. In conclusion, the QTLs for yield components in cotton can be mapped using CSILs and provide a base for breeding by design.

Key words: Chromosome segment introgression lines, Cotton, Lint percentage, Seed index, QTL

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