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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1512-1518.doi: 10.3724/SP.J.1006.2010.01512


Quality Improvement of Long-te-fu Male Sterile Lines with Antisense Wx Gene and Associated Application in Rice Breeding

WANG Xin-Qi, YIN Li-Qing, LU You-Lin, and SHEN Ge-Zhi*   

  1. Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
  • Received:2010-01-29 Revised:2010-04-18 Online:2010-09-12 Published:2010-06-11
  • Contact: SHEN Ge-Zhi,E-mail:Gzshen2003@yahoo.com.cn,Tel:021-62208184

Abstract: In this study, we have generated the Long-te-fu new maintain lines B (wxLB1, wxLB2) with the low amylose content (AC) in seeds of these lines by trans-antisense Wx gene, and obtained the corresponding new male sterile lines of Xin-long-bao A (wxLA1, wxLA2) by transferring breeding. The agronomic traits, amylose content, gel consistency (GC) and the RVA profile eigenvalue of hybrid offspring by the new sterile lines and the original male sterile lines crossed with restorers respectively were tested, the results showed that the amylose content and grain weight were extremely significant lower than the respective control, the gel consistency (GC) and breakdown viscosity (BDV) were higher than the respective control obviously, and reached the extremely significant level. And the cold viscosity (CPV), setback (SBV), peak time (PeT), pasting temperature (PaT) and consistence (CSV) were significant or extremely significant lower than the respective control. The analysis of variation source on gel consistency and RVA eigenvalue of hybrid offspring indicated that starch GC and the RVA eigenvalue mainly affected by the female parent and both reached to the extremely significant level. The integrative results showed that the improvement quality of the male sterile lines of Long-te-fu was an effective way to meliorate the starch physico-chemical and cooking quality of hybrid in indica rice.

Key words: Wx gene, Low amylase content, Male sterile line, Quality improvement, Indica

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