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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1683-1690.doi: 10.3724/SP.J.1006.2010.01683

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of TaG3BP Gene Related to Fertility Conversion in Male Sterile Lines of Wheat

ZHOU Lin-Lin1,SONG Guo-Qi1,LI Hong-Yan1,HU Yin-Gang1,2,3,*,HE Bei-Ru1   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Yangling Branch of China National Wheat Improvement Centre, Yangling 712100, Shaanxi; 3 Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
  • Received:2010-02-02 Revised:2010-04-21 Online:2010-10-12 Published:2010-07-05
  • Contact: HE Yin-Gang,E-mail:huyingang@126.com,huyingang@nwsuaf.edu.cn

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Abstract: Thermo-photoperiod sensitive male sterile (TMS) wheat (Triticum astivum L.) is a valuable material for heterosis study and utilization by means of two-line system. YS-type TMS lines are applicable in hybrid wheat in major wheat growing areas in northern China. The objective of this study was to reveal the molecular basis of fertility conversion in YS-type TMS wheat. YS-type TMS line A3017 was grown in a phytotron with controlled temperatures in favor of male sterility and fertility, respectively. Two suppression subtractive hybridization (SSH) cDNA libraries were constructed using cDNA from the male sterile and fertile young spikes of the same individuals that were orderly treated with temperature cycle (day/night) of 16℃/12℃ and 25℃/16℃. Using a pair of primers designed on the basis of aligned homological sequence, RT-PCR amplification was carried out and an EST with 1230 bp in length (GenBank accession number: DY543200) was obtained from the fertile SSH-cDNA library, which encodes 409 amino acid residues. The cDNA fragment contained the typical NTF2 and RRM domains of G3BP, and designated TaG3BP (GenBank accession number: GU475149). The deduced amino acids had 79% of similarity with G3BP (Ras-GTPase activating protein SH3 domain-binding protein) gene from Oryza sativa (BAD07751). The expression pattern of TaG3BP was analyzed with Real-time PCR using anthers from the TMS line in various developmental periods under male sterile and fertile conditions. In fertile anthers, TaG3BP gene was expressed in a higher level at the critical stage of fertility conversion (between uninucleate and binucleate stages) as compared with those in sterile anthers. In the K-type male-sterile lines and their maintainers of 1B/1R, 1B and non 1B/1R types, TaG3BP gene showed higher expression levels in the young spike of maintainers than in that of the male-sterile lines. These results suggest that the expression of TaG3BP gene is related to fertility conversion in male-sterile wheat lines.

Key words: Common wheat, Thermo-sensitive male sterility, Fertility conversion, G3BP, Gene expression analysis

[1] Sasakuma T, Ohtuka I. Cytoplasmic effects of Aegilops species having D genome in wheat: I. Cytoplasmic differentiation among five species regarding pistillody induction. Seiken Ziho, 1979, 27/28: 59-65
[2] He B-R(何蓓如), Dong P-H(董普辉), Song X-Y(宋喜悦). Study on the thermo- sensitivity of thermo-sensitive male-sterile wheat line A3314. J Triticeae Crop (麦类作物学报), 2003, 23(1): 1-6 (in Chinese with English abstract)
[3] Wang X D, Zhu Y G. mRNA differential display between sterile and fertile anther of rice and analysis of cDNA differential fragments. Sci China (Ser C), 1998, 41(2): 217-224
[4] Cao S-H(曹双河), Guo X-L(郭小丽), Liu D-C(刘冬成), Zhang X-Q(张相岐), Zhang A-M(张爱民). Preliminary gene-mapping of photoperiod- temperature sensitive genic male sterility in wheat (Triticum aestivum L.). Acta Genet Sin (遗传学报), 2004, 31(3): 293-298 (in Chinese with English abstract)
[5] Xing Q H, Ru Z G, Zhou C J, Xue X, Liang C Y, Yang D E, Jin D M, Wang B. Genetic analysis, molecular tagging and mapping of the thermo-sensitive genic male-sterile gene (wtms1) in wheat. Theor Appl Genet, 2003, 107: 1500-1504
[6] Xing Q H, Ru Z G, Li J, Zhou C J, Jin D M, Sun Y, Wang B. Cloning a second form of adenine phosphoribosy1 transferase gene (TaAPT2) from wheat and analysis of its association with thermo-senstive genic male sterility (TGMS). Plant Sci, 2005, 169: 37-45
[7] Guo R X, Sun D F, Cheng X D, Rong D F, Li C D. Inheritance of thermo-photoperiod sensitive male sterility in wheat. Aust J Agric Res, 2006, 57: 187-192
[8] Guo R X, Sun D F, Tan Z B, Rong D F, Li C D. Two recessive genes controlling thermophotoperiod-sensitive male sterility in wheat. Theor Appl Genet, 2006, 112: 1271-1276
[9] Zhao C-P(赵昌平), Zhang L-P(张立平), Li Y-F(李云伏), Ma R-C(马荣才), Shan F-H(单福华), Zhang F-T(张风廷), Ye Z-J(叶志杰), Qin N(秦娜). Analysis of photoperiod-temperature senstive male sterility relative genes in wheat with DDRT-PCR. Chin J Biochem Mol Biol (中国生物化学与分子生物学报), 2007, 23(1): 56-62 (in Chinese with English abstract)
[10] Chen J-F(陈军方), Ren Z-L(任正隆), Kong X-Y(孔秀英), Wu J-J(吴佳洁), Zhou R-H(周荣华), Jia J-Z(贾继增). Isolation, characterization and expression analysis of male sterility gene homology sequence in wheat. Acta Genet Sin (遗传学报), 2005, 32(6): 566-570 (in Chinese with English abstract)
[11] Dong J-H(董玖红), Wen J-F(文建凡).The structure and function of small GTPase. Chem life (生命的化学), 2005, 25(5): 422-424 (in Chinese)
[12] Bischoff F, Molendijk A, Rajendrakumar C S V, Palme K. GTP-binding proteins in plants. Cell Mol Life Sci, 1999, 55: 233-256
[13] Parker F, Maurier F, Delumeau I, Duchesne M, Faucher D, Debussche L, Dugue A, Schweighoffer F, Tocque B. ARas GTPase activating protein SH3 domain binding protein. Mol Cell Biol, 1996, 16: 2561-2569
[14] Gallouzi I E, Parker F, Chebli K, Maurier F, Labourier E, Barlat I, Capony J P, Tocque B, Tazi J. A novel phosphorylation dependent RNase activity of GAP SH3 binding protein: a potential link between signal transduction and RNA stability. Mol Cell Biol, 1998, 18: 3956-3965
[15] Guitard E, Parker F, Millon R, Abecassis J, Tocque B. G3BP is overexpressed in human tumors and promotes S phase entry. Cancer Lett, 2001, 162: 213-221
[16] Costa M, Ochem A, Staub A, Falaschi A. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the Ras transduction pathway. Nucl Acids Res, 1999, 27: 817-821
[17] Duchesne M, Schweighoffer F, Parker F, Clerc F,Frobert Y, Thang M N, Tocque B.Identification of the SH3 domain of GAP as an essential sequence for Ras GAP mediated signaling. Science, 1993, 259: 525-528
[18] Katharine I, Renee S, David H, Derek K. Rasputin, more promiscuous than ever: a review of G3BP. Int J Dev Biol, 2004, 48: 1065-1077
[19] Song G-Q(宋国琦), Hu Y-G(胡银岗), Lin F-Y(林凡云), Dong P-H(董普辉), He B-R(何蓓如). cDNA-AFLP analysis of fertility-alteration genes in a thermo-sensitive male sterile line of YS wheat. Acta Bot Boreal-Occiden Sin (西北植物学报), 2006, 26(4): 661-666 (in Chinese with English abstract)
[20] Song G-Q(宋国琦), Hu Y-G(胡银岗), Lin F-Y(林凡云), Dong P-H(董普辉), He B-R(何蓓如).SSH analysis of fertility-related genes of YS type thermo-sensitive male sterile line A3017. Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(7): 1031-1038 (in Chinese with English abstract)
[21] Zhou L-L(周琳璘), Song G-Q(宋国琦), Li H-Y(李红燕), Hu Y-G(胡银岗), He B-R(何蓓如). A MADS-Box transcription factor related to fertility conversion in male sterile wheat lines. Acta Agron Sin (作物学报), 2008, 34(4): 598-604 (in Chinese with English abstract)
[22] Li H-Y(李红燕), Zhou L-L(周琳璘), He B-R(何蓓如), Hu Y-G(胡银岗).Analysis on the genes related to fertility conversion in wheat thermo-sensitive male-sterile line YM3314. Acta Bot Boreal-Occident Sin (西北植物学报), 2009, 29(11): 2181-2188 (in Chinese with English abstract)
[23] Zhang C-Y(张驰宇), Xu S-G(徐顺高), Huang X-X(黄新祥). A novel and convenient relative quantitative method of fluorescence real time RT-PCR assay based on slope of standard curve. Prog Biochem Biophys (生物化学与生物物理进展), 2005, 32(9): 883-888 (in Chinese with English abstract)
[24] Huang Q-L(黄庆榴), Tang X-H(唐锡华), Mao J-L(茅剑蕾). Photoperiodic and temperature responses of a japonica photoperiod-sensitive male-sterile line 7001s and their effect on fertility and anther protein. Acta Agron Sin (作物学报), 1994, 20(3): 156-160 (in Chinese with English abstract)
[25] Wang Z H, Zou Y J, Li X Y, Zhang Q Y, Chen L T, Wu H, Su D H, Chen Y L, Guo J X, Luo D, Long Y M, Zhong Y, Lin Y G. Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell, 2006, 18: 676-687
[26] Hou S-Y(侯思宇), Zhang R(张锐), Guo S-D(郭三堆). Cloning and expression profile analysis of GhPG2 gene associated with fertilityin cotton (Gossypium hirsutum L.). Acta Agron Sin (作物学报), 2009, 35(11): 2008-2014 (in Chinese with English abstract)
[27] Chang L, Ma H, Xue H W. Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice. Cell Res, 2009, 19: 768-782
[28] McCudden C R, Hains M D, Kimple R J, Siderovski D P, Willard F S. G-protein signaling: back to the future. Cell Mol Life Sci, 2005, 62: 551-577
[29] Briggs S D, Bryant S S, Jove R, Sanderson S D, Smithgall T E. The Ras GTPase-activating protein (GAP) is an SH3 domain-binding protein and substrate for the Src-related tyrosine kinase, Hck. J Biol Chem, 1995, 270: 14718-14724
[30] Kennedy D, Wood S A, Ramsdale T, Tam P P, Steriner K A, Mattick J S. Identification of a mouse orthologue of the human ras-GAP-SH3-domain binding protein and structural confirmation that these proteins contain an RNA recognition motif. Biomed Pept Proteins Nucl Acids, 1996, 2: 93-99
[31] Tocque B, Delumeau I, Parker F, Maurier F, Multon M C, Schweighoffer F. Ras-GTPase activating protein (GAP): a putative effector for Ras. Cell Signal, 1997, 9: 153-158 Barnes C J, Li F, Mandal M, Yang Z B, Sahin A A, Kumar R. Heregulin induces expression, ATPase activity, and nuclear localization of G3BP, a Ras signaling component, in human breast tumors. Cancer Res, 2002, 62: 1251-1255
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