Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1605-1609.doi: 10.3724/SP.J.1006.2010.01605

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Cloning and Activity Analysis of Zea mays ZmPR4 Promoter in Wheat Immature Embryonic Cali

WANG Ai-Yun1,2, ZHUANG Hong-Tao2,3,**,ZHANG Zeng-Yan2,*,ZHANG Xue-Wen3,DU Li-Pu2,YE Xing-Guo2   

  1. 1 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;     3 College of Life Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-05-04 Revised:2010-07-19 Online:2010-09-12 Published:2010-08-25
  • Contact: ZHANG Zeng-Yan, E-mail: zhangzy@mail.caas.net.cn

PDF

1960

Cited

2

Abstract: The ZmPR4 promoter was cloned from genomic DNA of maize inbred line B37 through specific PCR amplification. The promoter cloned had the same sequence as the fragment registered in GenBank under the accession number of AJ969166. Expression vectors driven by ZmPR4 promoter or Ubiquitin promoterwere constructed, which harbor GUS gene. The vectors were bombarded into wheat immature embryogenic calli. Histochemical assays of GUS activity, the ZmPR4 promoter was obviously expressed in wheat immature embryonic calli, but the expression activity was lower than that of Ubi Promoter. The ZmPR4 promoter could be induced by infection of Rhizoctonia cerealis. PCR assay confirmed the expression of GUS gene driven by ZmPR4 promoter. These results suggest that ZmPR4promoter has a use potential in molecular breeding of resistance in wheat.

Key words: Inducible promoter, Cloning, Calli, Transient expression

[1] Chen Y-X(陈延熙), Tang W-H(唐文华), Zhang D-H(张敦华), Jian X-Y(简小鹰). A preliminary study on etiology of sharp eyespot of wheat in China. J Plant Prot (植物保护学报), 1986, 13(1): 39-44 (in Chinese with English abstract)
[2] Shi J-R(史建荣), Wang Y-Z(王裕中), Shen S-W(沈素文), Chen H-G(陈怀谷).Pathogenicity of Rhizoctonia cerealis to wheat in Jiangsu province. Jiangsu J Agric Sci (江苏农业学报), 1997, 13(3): 188-190 (in Chinese with English abstract)
[3] Wang Y-Z(王裕中). Occurrence of wheat sharp eyespot and its controlling. Plant Prot Technol Extension (植保技术与推广), 2001, 21(8): 39-41 (in Chinese)
[4] Mitsuhara I, Matsufuru H, Ohshima M, Kaku H, Nakajima Y, Murai N, Natori S, Ohashi Y. Induced expression of sarcotoxin IA enhanced host resistance against both bacterial and fungal patho- gens in transgenic tobacco
[J].Mol Plant-Microbe Interact
[5] Osusky M, Zhou G, Osuska L, Hancock R E, Kay W W, Misra A. Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens
[J].Nat Biotech
[6] Sharma A, Sharma R, Imamura M, Yamakawa M, Machii H. Transgenic expression of cecropin B, an antibacterial peptide from Bombyx mori, confers enhanced resistance to bacterial leaf blight in rice
[J].FEBS Lett
[7] Li Q, Lawrence C B, Xing H Y, Babbitt R A, Bass W T, Maiti I B, Everett N P. Enhanced disease resistance conferred by expression of an antimicrobial magainin analog in transgenic tobacco. Planta, 2001, 212: 635-639
[8] Carmona, M J, Molina A, Fernández, J A, López-Fando J J, García-Olmedo F. Expression of the a-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens
[J].Plant J
[9] Mourgues F, Brisset M N, Chevreau E. Strategies to improve plant resistance to bacterial diseases through genetic engineering
[J].Trends Biotech
[10] Coca M, Bortolotti C, Rufat M, Peñas G, Eritja R, Tharreau D, Martinez del Pozo A, Messeguer J, San Segundo B. Transgenic rice plants expressing the antifungal AFP protein from Aspergillus giganteus show enhanced resistance to the rice blast fungus Magnaporthe grisea
[J].Plant Mol Biol
[11] Iwai T, Kaku H, Honkura R, Nakamura S, Ochiai H, Sasaki T, Ohashi Y. Enhanced resistance to seedtransmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall- bound thionin
[J].Mol Plant-Microbe Interact
[12] Kanzaki H, Nirasawa S, Saitoh H, Ito M, Nishihara M, Terauchi R, Nakamura I. Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus (Magnaporthe grisea) in transgenic rice
[J].Theor Appl Genet
[13] Jia S-R(贾士荣). Environment and food biosafety assessment of trangenic plants. Adv Bioeng (生物工程进展), 1997, 17(6): 37-42 (in Chinese with English abstract)
[14] Morris S H, Adley C C. Irish public perceptions and attitudes to modern biotechnology: an overview with a focus on GM foods
[J].Trends Biotechnol
[15] Simth N. Seeds of opportunity an assessment of the benefits, safety and oversight of plant genomics and agricultural biotechnology. Biotechnology Law Report. August 2000, 19(4): 449-536.
[16] Moreno A B, Peñas G, Rufat M, Bravo M J, Estopà M, Messeguer J, Segundo B S. Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice
[J].Mol Plant- Microbe Interact
[17] Jefferson R A. Assaying chimeric genes in plants: the GUS gene fusion system
[J].Plant Mol Biol Rep
[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[3] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[4] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[5] YUE Jie-Ru, BAI Jian-Fang, ZHANG Feng-Ting, GUO Li-Ping, YUAN Shao-Hua, LI Yan-Mei, ZHANG Sheng-Quan, ZHAO Chang-Ping, ZHANG Li-Ping. Cloning and potential function analysis of ascorbic peroxidase gene of hybrid wheat in seed aging [J]. Acta Agronomica Sinica, 2021, 47(3): 405-415.
[6] LI Jing-Lin, LI Jia-Lin, LI Xin-Peng, AN Bao-Guang, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jing, LONG Tuan. Creation and combining ability analysis of recessive genic sterile lines with a new ptc1 locus in rice [J]. Acta Agronomica Sinica, 2021, 47(11): 2173-2183.
[7] YANG Qin-Li, YANG Duo-Feng, DING Lin-Yun, ZHANG Ting, ZHANG Jun, MEI Huan, HUANG Chu-Jun, GAO Yang, YE Li, GAO Meng-Tao, YAN Sun-Yi, ZHANG Tian-Zhen, HU Yan. Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes [J]. Acta Agronomica Sinica, 2021, 47(10): 1854-1862.
[8] HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao. Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate [J]. Acta Agronomica Sinica, 2021, 47(10): 1941-1952.
[9] Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU. Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea [J]. Acta Agronomica Sinica, 2020, 46(12): 1850-1861.
[10] XUE Xiao-Meng,LI Jian-Guo,BAI Dong-Mei,YAN Li-Ying,WAN Li-Yun,KANG Yan-Ping,HUAI Dong-Xin,LEI Yong,LIAO Bo-Shou. Expression profiles of FAD2 genes and their responses to cold stress in peanut [J]. Acta Agronomica Sinica, 2019, 45(10): 1586-1594.
[11] Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG. Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1021-1031.
[12] Rui-Juan YANG,Jian-Rong BAI,Lei YAN,Liang SU,Xiu-Hong WANG,Rui LI,Cong-Zhuo ZHANG. Cloning and Expression Analysis of Strong Inducible Promoter P1502-ZmPHR1 Responding to Low Phosphorus Stress in Maize [J]. Acta Agronomica Sinica, 2018, 44(7): 1000-1009.
[13] Chen-Yu MA,Wei-Min ZHAN,Wen-Liang LI,Meng-Di ZHANG,Zhang-Ying XI. Cloning and Function Analysis of ZmNAOD Gene in Maize [J]. Acta Agronomica Sinica, 2018, 44(10): 1433-1441.
[14] Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI. Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm [J]. Acta Agronomica Sinica, 2018, 44(03): 376-384.
[15] Yun-Fei LIANG, Lin-Cheng ZHANG, Quan-Ming PU, Zhen-Ze LEI, Song-Mei SHI, Yu-Peng JIANG, Xue-Song REN, Qi-Guo GAO. Cloning of BoLH27 Gene from Cabbage and Phenotype Analysis of Transgenic Cabbage [J]. Acta Agronomica Sinica, 2018, 44(03): 397-404.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd