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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 587-594.doi: 10.3724/SP.J.1006.2011.00587

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of Cold-Inducible Gene SlCMYB1 and Its Heterologous Expression in Rice

  

  • Received:2010-10-28 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 万建民, E-mail: wanjm@caas.net.cn

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Abstract: We isolated a cDNA clone, designated SlCMYB1 (for Solanum lycopersicum Cold MYB 1), from tomato by analyzing the previous microarray data of the low temperature transcriptome of tomato. SlCMYB1 encodes a R2R3-MYB family gene, which contains a conserved DNA-binding domain composed of two repeat motifs. The SlCMYB1-GFP fusion protein was localized to the nucleus in a transient expression assay. Expression of SlCMYB1 was not only strongly induced by cold and salt, but also by dehydration and abscisic acid (ABA). To understand function of SlCMYB1 in crop plants under stress conditions, we generated transgenic plants in rice over-expressing SlCMYB1 under control of the CaMV 35S promoter. Preliminary results showed that overexpression of SlCMYB1 in rice induced constitutive expression of the cold-responsive gene OsP5CS1 and OsWCOR413 and might be conferred enhanced tolerance to cold treatments for non-acclimated transgenic plants, compared with wild type plants. Our data suggest that SlCMYB1 might play an important role in plant responses to cold stress by regulating cold-responsive gene expression and might be a useful gene for improving cold tolerance in crop plants.

Key words: R2R3-type MYB transcriptional factor, SlCMYB1, Rice, Cold tolerance

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