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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1497-1502.doi: 10.3724/SP.J.1006.2011.01497

• RESEARCH NOTES • Previous Articles    

Cloning and Sequence Analysis of α/β-gliadin Genes from Common Wheat Variety Xiaoyan 54

ZHANG Xiao-Xia1,2,JIAO Zhen1,DONG Zhen-Ying2,LI Shi-Ming2,WANG Ran2,LING Hong-Qing2,QIN Guang-Yong1,*,WANG Dao-Wen2,*   

  1. 1 Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; 2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2011-01-25 Revised:2011-03-26 Online:2011-08-12 Published:2011-06-13
  • Contact: 秦广雍, E-mail: qinguangyong@zzu.edu.cn; 王道文, E-mail: dwwang@genetics.ac.cn

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Abstract: We developed a method for identifying the genomic BAC clones containing the α/β-gliadin gene sequences of common wheat (Triticum aestivum L.). Using this method, nine unique positive clones harboring α/β-gliadin genes were obtained from the BAC library of the elite wheat variety Xiaoyan 54. From these BAC clones, 17 distinct α/β-gliadin gene sequences were isolated, 12 of which were pseudogenes because of the presence of one or more premature stop codons in their open reading frame (ORF). The remaining five genes (tentatively designated Gli-Xy54-1, Gli-Xy54-2, Gli-Xy54-3, Gli-Xy54-7, and Gli-Xy54-13) possessed intact ORF. Their deduced protein sequences contained 291, 310, 311, 287, and 317 amino acid residues, respectively, and resembled highly the primary structure of previously reported α/β-gliadins. The presence of celiac disease inducing epitopes and the length of the polyglutamine repeats in the five deduced α/β-gliadins were analyzed. Based on these data, it was suggested that Gli-Xy54-1 might be located on chromosome 6A, Gli-Xy54-2, Gli-Xy54-3, and Gli-Xy54-13 on chromosome 6B, and Gli-Xy54-7 on chromosome 6D. This proposition was supported by phylogenetic analysis of the gliadin gene sequences isolated here and previously. As far as we know, this work represents the first report on the identification of BAC clones containing the α/β-gliadin genes from common wheat. The genes isolated here may facilitate further investigations on the composition, expression and function of α/β-gliadin genes in common wheat.

Key words: Common wheat, α/β-gliadin, BAC library screening, Gene cloning

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