Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1497-1502.doi: 10.3724/SP.J.1006.2011.01497

• RESEARCH NOTES • Previous Articles    

Cloning and Sequence Analysis of α/β-gliadin Genes from Common Wheat Variety Xiaoyan 54

ZHANG Xiao-Xia1,2,JIAO Zhen1,DONG Zhen-Ying2,LI Shi-Ming2,WANG Ran2,LING Hong-Qing2,QIN Guang-Yong1,*,WANG Dao-Wen2,*   

  1. 1 Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; 2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2011-01-25 Revised:2011-03-26 Online:2011-08-12 Published:2011-06-13
  • Contact: 秦广雍, E-mail: qinguangyong@zzu.edu.cn; 王道文, E-mail: dwwang@genetics.ac.cn

PDF

1444

Abstract: We developed a method for identifying the genomic BAC clones containing the α/β-gliadin gene sequences of common wheat (Triticum aestivum L.). Using this method, nine unique positive clones harboring α/β-gliadin genes were obtained from the BAC library of the elite wheat variety Xiaoyan 54. From these BAC clones, 17 distinct α/β-gliadin gene sequences were isolated, 12 of which were pseudogenes because of the presence of one or more premature stop codons in their open reading frame (ORF). The remaining five genes (tentatively designated Gli-Xy54-1, Gli-Xy54-2, Gli-Xy54-3, Gli-Xy54-7, and Gli-Xy54-13) possessed intact ORF. Their deduced protein sequences contained 291, 310, 311, 287, and 317 amino acid residues, respectively, and resembled highly the primary structure of previously reported α/β-gliadins. The presence of celiac disease inducing epitopes and the length of the polyglutamine repeats in the five deduced α/β-gliadins were analyzed. Based on these data, it was suggested that Gli-Xy54-1 might be located on chromosome 6A, Gli-Xy54-2, Gli-Xy54-3, and Gli-Xy54-13 on chromosome 6B, and Gli-Xy54-7 on chromosome 6D. This proposition was supported by phylogenetic analysis of the gliadin gene sequences isolated here and previously. As far as we know, this work represents the first report on the identification of BAC clones containing the α/β-gliadin genes from common wheat. The genes isolated here may facilitate further investigations on the composition, expression and function of α/β-gliadin genes in common wheat.

Key words: Common wheat, α/β-gliadin, BAC library screening, Gene cloning

[1]Han B(韩彬), Shepherd K W. The correlations between low glutenin subunits and gliadins and their effects on bread-making quality in the progeny of two wheats. Sci Agric Sin (中国农业科学), 1991, 24(4): 19–25 (in Chinese with English abstract)
[2]Bushuk W, Zillman R R. Wheat cultivar identification by gliadin electrophoregrams. Can J Plant Sci, 1978, 58: 505–515
[3]Qi P F, Wei Y M, Yue Y W, Yan Z H, Zheng Y L. Biochemical and molecular characterization of gliadins. Mol Biol, 2006, 40: 713–723
[4]Kasarda D D, Autran J C, Lew E J L, Nimmo C C, Shewry P R. N-terminal amino acid sequences of ω-gliadins and ω-secalins: implications for the evolution of prolamin genes. Biochim Biophys Acta, 1983, 747: 138–150
[5]Bietz J A, Huebner F R, Sanderson J E, Wall J S. Wheat gliadin homology revealed through N-terminal amino acid sequence analysis. Cereal Chem, 1977, 54: 1070–1083
[6]Payne P I. Genetics of wheat storage proteins and the effect of allelic variation on bread mapping quality. Annu Rev Plant Physiol, 1987, 38: 141–153
[7]Müller S, Wieser H. Disulphide bonds of alpha-type gliadins. J Cereal Sci, 1995, 22: 21–27
[8]Müller S, Wieser H. The location of disulphide bonds in monomeric gamma-gliadins. J Cereal Sci, 1997, 26: 169–176
[9]Redaelli R. Two-dimension mapping of gliadins using biotypes and null mutants of common wheat cultivar Savatovskaya 29. Hereditas, 1994, 121: 131–137
[10]Kasarda D D, Okita T W, Bernardin J E, Baecker P A, Nimmo C C, Lew E J L, Dietler M D, Greene F C. Nucleic acid (cDNA) and amino acid sequences of α-type gliadins from wheat (Triticum aestivum). Proc Natl Acad Sci USA, 1984, 81: 4712–4716
[11]Dewar D, Pereira S P, Ciclitira P J. The pathogenesis of celiac disease. Int J Biochem Cell Biol , 2004, 36: 17–24
[12]Londei M, Ciacci C, Ricciardelli I, Vacca L, Quaratino S, Maiuri L. Gliadin as a stimulator of innate responses in celiac disease. Mol Immunol, 2005, 42: 913–918
[13]Van Herpen T W J M, Goryunova S V, van der Schoot J, Mitreva M, Salentijn E, Vorst O, Schenk M F, van Veelen P A, Koning F, van Soest L J M, Vosman B, Bosch D, Hamer R J, Gilissen L J W J, Smulders M J M. Alpha-gliadin genes from the A, B, and D genomes of wheat contain different sets of celiac disease epitopes. BMC Genomics, 2006, 7: 1
[14]Lee Y K, Békés F, Gras P, Ciaffi M, Morell M K, Appels R. The-low-molecular-weight glutenin subunit proteins of primitive wheats: IV. Functional properties of products from individual genes. Theor Appl Genet, 1999, 98: 149–155
[15]Dong L L, Zhang X F, Liu D C, Fan H J, Sun J Z, Zhang Z J, Qin H J, Li B, Hao S T, Li Z S, Wang D W, Zhang A M, Ling H Q. New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS ONE, 2010, 5(10): e13548
[16]Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24: 1596–1599
[17]Liu Q(刘千), Long H(龙海), Wei Y-M(魏育明), Yan Z-H(颜泽洪), Zheng Y-L(郑有良). Sequence analysis of a-gliadin genes from wheat variety “Chuannong 16”. Sci Agric Sin (中国农业科学), 2008, 41(7): 2168–2173 (in Chinese with English abstract)
[18]Chen H-P(陈华萍), Long H(龙海), Liu Q(刘千), Wei Y-M(魏育明), Zheng Y-L(郑有良). Sequence analysis of α/β-gliadin genes from Sichuan wheat landrace AS1643. Sci Agric Sin (中国农业科学), 2006, 39(9): 1743–1750 (in Chinese with English abstract)
[19]Gupta R B, MacRitchie F. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats: II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci, 1994, 19: 19–29
[20]Spaenij-Dekking L, Kooy-Winkelaar Y, van Veelen P, Wouter Drijfhout J, Jonker H, van Soest L, Smulders M J M, Bosch D, Gilissen L J W J, Koning F. Natural variation in toxicity of wheat: potential for selection of nontoxic varieties for celiac disease patients. Gastroenterology, 2005, 129: 797–806
[21]Anderson O D, Litts J C, Greene F C. The α-gliadin gene family: I. Characterization of ten new wheat α-gliadin genomic clones, evidence for limited sequence conservation of flanking DNA, and southern analysis of the gene family. Theor Appl Genet, 1997, 95: 50–58
[22]Okita T W, Cheesbrough V, Reeves C D. Evolution and heterogeneity of the alpha-/beta-type and gamma-type gliadin DNA sequences. J Biol Chem, 1985, 260: 8203–8213
[23]Harberd N P, Bartels D, Thompson R D. Analysis of the gliadin multigene locus in bread wheat using nullisomic-tetrasomic lines. Mol Gen Genet, 1985, 198: 234–242
[24]Ma Z C, Wei Y M, Yan Z H, Zheng Y L. Characterization of alpha-gliadin genes from diploid wheats and the comparative analysis with those from polyploidy wheats. J Genet, 2007, 43: 1534–1541
[25]Zhu X-P(朱西平), Li X(李鑫), Li Y-X(李雅轩), Yan Y-M(晏月明). Cloning, chromosomal location, and evolutionary analysis of α-gliadin genes from Aegilops tauschii and common wheat (Triticum aestivum L.). Acta Agron Sin (作物学报). 2010, 36(4): 580–589 (in Chinese with English abstract)
[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[3] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[4] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[5] JIN Yi-Rong, LIU Jin-Dong, LIU Cai-Yun, JIA De-Xin, LIU Peng, WANG Ya-Mei. Genome-wide association study of nitrogen use efficiency related traits in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 394-404.
[6] YUE Jie-Ru, BAI Jian-Fang, ZHANG Feng-Ting, GUO Li-Ping, YUAN Shao-Hua, LI Yan-Mei, ZHANG Sheng-Quan, ZHAO Chang-Ping, ZHANG Li-Ping. Cloning and potential function analysis of ascorbic peroxidase gene of hybrid wheat in seed aging [J]. Acta Agronomica Sinica, 2021, 47(3): 405-415.
[7] YANG Qin-Li, YANG Duo-Feng, DING Lin-Yun, ZHANG Ting, ZHANG Jun, MEI Huan, HUANG Chu-Jun, GAO Yang, YE Li, GAO Meng-Tao, YAN Sun-Yi, ZHANG Tian-Zhen, HU Yan. Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes [J]. Acta Agronomica Sinica, 2021, 47(10): 1854-1862.
[8] HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao. Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate [J]. Acta Agronomica Sinica, 2021, 47(10): 1941-1952.
[9] ZHANG Ping-Ping,YAO Jin-Bao,WANG Hua-Dun,SONG Gui-Cheng,JIANG Peng,ZHANG Peng,MA Hong-Xiang. Soft wheat quality traits in Jiangsu province and their relationship with cookie making quality [J]. Acta Agronomica Sinica, 2020, 46(4): 491-502.
[10] Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU. Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea [J]. Acta Agronomica Sinica, 2020, 46(12): 1850-1861.
[11] Di JIN,Dong-Zhi WANG,Huan-Xue WANG,Run-Zhi LI,Shu-Lin CHEN,Wen-Long YANG,Ai-Min ZHANG,Dong-Cheng LIU,Ke-Hui ZHAN. Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat [J]. Acta Agronomica Sinica, 2019, 45(6): 807-817.
[12] Fang-Ping YANG,Jin-Dong LIU,Ying GUO,Ao-Lin JIA,Wei-E WEN,Kai-Xiang CHAO,Ling WU,Wei-Yun YUE,Ya-Chao DONG,Xian-Chun XIA. QTL mapping of adult-plant resistance to stripe rust in wheat variety holdfast [J]. Acta Agronomica Sinica, 2019, 45(12): 1832-1840.
[13] XUE Xiao-Meng,LI Jian-Guo,BAI Dong-Mei,YAN Li-Ying,WAN Li-Yun,KANG Yan-Ping,HUAI Dong-Xin,LEI Yong,LIAO Bo-Shou. Expression profiles of FAD2 genes and their responses to cold stress in peanut [J]. Acta Agronomica Sinica, 2019, 45(10): 1586-1594.
[14] Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG. Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1021-1031.
[15] Chen-Yu MA,Wei-Min ZHAN,Wen-Liang LI,Meng-Di ZHANG,Zhang-Ying XI. Cloning and Function Analysis of ZmNAOD Gene in Maize [J]. Acta Agronomica Sinica, 2018, 44(10): 1433-1441.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd