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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (10): 1794-1800.doi: 10.3724/SP.J.1006.2011.01794

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analyzing Synchronization Process of Mitosis Cells in Wheat Root-tip with Flow Karyotype

GUO Dong-Wei1,ZHANG Ren-He1,LI Chun-Lian1,CHEN Yao-Feng1,MIN Dong-Hong1,*,CHEN Ming2,LI Lian-Cheng2,XU Zhao-Shi2,MA You-Zhi2   

  1. 1 Agronomy College, Northwest A & F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-01-13 Revised:2011-06-25 Online:2011-10-12 Published:2011-07-28
  • Contact: 闵东红, E-mail: mdh2493@nwsuaf.edu.cn E-mail:gdwei1973@126.com

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Abstract: Genomic sequencing of wheat can be simplified through constructing chromosome specific library. Synchronization of cells is propitious to high mitosis index (MI), which results in the accumulation of metaphase chromosomes and a successful flow sorting. In the present study, we used a double-blocked strategy in the synchronization treatment of wheat (Triticum aestivum L. cv. Chinese Spring) root-tip cells with 50 µmol L-1 hydroxyurea (HU) and 100 µmol L-1 trifluralin. The effects of treatments were evaluated by analyzing the dynamic proportions of cells at different stages of cell cycle. The optimal hours of treatments were 10 h for HU block, 7 h for recovery, and 4 h for trifluralin block. According to this synchronization scheme, the maximum MI was 70.1%. Additional ice water (0°C) depression was helpful for decreasing chromosome debris and rising resolution of the flow karyotype, but had no effect on the accumulation of metaphase chromosomes in root-tip cells.

Key words: Wheat, Mitosis, Synchronization, Flow karyotype, Flow sorting

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