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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 186-189.doi: 10.3724/SP.J.1006.2012.00186

• RESEARCH NOTES • Previous Articles    

Establishment of a HPLC-UV Method for Simultaneous Determination of DON, 15ACDON, and 3ACDON in Wheat

YANG Dan,GENG Zhi-Ming,MA Hong-Xiang,YAO Jin-Bao,ZHANG Xu,ZHANG Ping-Ping,ZHANG Peng   

  1. Provincial Key Laboratory of Agrobiology / Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2011-05-27 Revised:2011-09-18 Online:2012-01-12 Published:2011-09-30
  • Contact: 马鸿翔, E-mail: mahx@jaas.ac.cn

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Abstract: A HPLC-UV method was established to determine deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ACDON) and 15- acetyldeoxynivalenol (15-ACDON) in wheat. Wheat sample was extracted by water, and the extracting solution was precipitated with an equal volume of ethanol followed by solid phase extraction with Oasis HLB cartridge. Qualitative and quantitative analyses of DON, 15ACDON and 3ACDON were done by HPLC-UVD using gradient elution program with acetonitrile and 0.005% phosphoric acid solution. Results showed that at the linear range of 0.5–15.0 mg L-1, the recoveries were 89.8%, 93.4%, and 92.9%, respectively. The relative standard deviations were 2.2%, 2.0%, and 2.5%, respectively, and the detection limit levels were 12.2, 10.5, and 16.7 μg kg-1, respectively. The method was accurate and reproducible. Sample purification lessened interference peaks, and due to the use of organic solvents was greatly reduced, the cost was decreased. Therefore, the method was suitable for the large batch determination of type B trichothecenes in wheat.

Key words: Wheat, B trichothecenes, HPLC-UV

[1]Cavaliere C, D’Ascenzo G, Foglia P, Pastorini E, Samperi R, Laganà A. Determination of type B trichothecenes and macrocyclic lactone mycotoxins in field contaminated maize. Food Chem, 2005, 92: 559–568
[2]Wang Y-Z(王裕中), Miller J D. Toxin producing potential of Fusarium graminearum from China. Mycosystema (真菌学报), 1994, 13(3): 229–234 (in Chinese with English abstract)
[3]Zhang P(张鹏), Ma H-X(马鸿翔). Biosynthesis and regulation of trichothecenes in Fusarium species. Biotechnol Bull (生物技术通报), 2009, 1: 11–15 (in Chinese with English abstract)
[4]FAO/WHO, Summary report of the seventy-second meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), issued 16th March 2010, available at http://www.who.int/foodsafety/chem/summary72 rev.pdf
[5]Wolf C E, Bullerman L B. Heat and pH alter the concentration of deoxynivalenol in an aqueous environment. Food Prot, 1998, 61: 365–367
[6]Wang Y-Z(王裕中), Chen H-G(陈怀古), Yang X-Y(杨新宁), Lu M(陆鸣), Wu Z-F(吴志凤). Bioactivity of crude toxin produced by Fusarium graminearum and its application in identification of fusarium head blight resistance of wheat cultivars. China Agric Sci (中国农业科学), 1989, 2(4): 54–57 (in Chinese with English abstract)
[7]Wei R-Y(魏润蕴). Thin-layer chromatography determining of deoxynivalenol in wheat. J Hyg Res (卫生研究), 1986, 15(5): 40–43 (in Chinese)
[8]Trucksess M W, Page S W, Wood G E, Cho T H. Determination of deoxynivalenol in white flour, whole wheat flour, and bran by solid-phase extraction/liquid chromatography: interlaboratory study. J AOAC Int, 1998, 81: 880–886
[9]Scott P M, Kanhere S R. Comparison of column phases for separation of derivatized trichothecenes by capillary gas chromatography. J Chromatogr, 1986, 368: 374–380
[10]Tacke B K, Casper H H. Determination of deoxynivalenol in wheat, barley, and malt by column cleanup and gas chromatography with electron capture detection. J AOAC Int, 1996, 79: 472–475
[11]Jestoi M, Ritieni A, Rizzo A. Analysis of the Fusarium mycotoxins fusaproliferin and trichothecenes in grains using gas chromatography-mass spectrometry. J Agric Food Chem, 2004, 52: 1464–1469
[12]Klötzel M, Gutsche B, Lauber U, Humpf H U. Determination of 12 type A and B trichothecenes in cereals by liquid chromatography electrospray ionization tandem mass spectrumetry. J Agric Food Chem, 2005, 53: 8904–8910
[13]Park J J, Chu F S. Assessment of mimunochemical methods for the analysis of trichothecene mycotoxins in naturally occur-ringmoldy corn. J AOAC Int, 1996, 79: 465–471
[14]Casale W L, Pestka J J, Hart L P. Enzyme-linked mimunosor-bentassay employingmonoclonalantibody specific for deoxynivalenol (vomitoxin) and sever alanalogues. J Agric Food Chem, 1988, 36: 663–668
[15]Laamanen I, Veijalainen P. Factors affecting the results of T-2 mycotoxin ELISA assay. J Food Addit Contam, 1992, 9: 337–343
[16]Turner N W, Subrahmanyam S, Piletsky S A. Analytical methods for determination of mycotoxins: a review. Anal Chim Acta, 2009, 632: 168–180
[17]Klötzel M, Schmidt S, Lauber U, Thielert G, Humpf H U. Comparison of different clean-up procedures for the analysis of deoxynivalenol in cereal-based food and validation of a reliable HPLC method. Chromatographia, 2005, 62: 41–48
[18]Jiménez M, Mateo R. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and highperformance liquid chromatography. J Chromatogr A, 1997, 778: 363–372
[19]Luongo D, Severino L, Bergamo P, D’Arienzo R, Rossi M. Trichothecenes NIV and DON modulate the maturation of murine dendritic cells. Toxicon, 2010, 55: 73–80
[20]Yasuaki D, Sayaka K, Masaomi K, Yukie S, Tomoaki H, Keisuke S, Kunitoshi M, Susumu K, Yoshiko S K, Makoto S. Rapid deposition of glomerular IgA in BALB/c mice by nivalenol and its modifying effect on high IgA strain (HIGA) mice. Exp Toxicol Pathol, 2011, 63: 17–24
[21]Bony S, Olivier-Loiseau L, Carcelen M, Devaux A. Genotoxic potential associated with low levels of the Fusarium mycotoxins nivalenol and fusarenon X in a human intestinal cell line. Toxicol in Vitro, 2007, 21: 457–465
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